| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,462,437 | G→T | 6.5% | A259E (GCA→GAA) ‡ | chiA ← | periplasmic endochitinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,462,437 | 0 | G | T | 6.5% | 3114.5 / 31.5 | 980 | A259E (GCA→GAA) ‡ | chiA | periplasmic endochitinase |
| Reads supporting (aligned to +/- strand): ref base G (256/629); new base T (2/93); total (258/722) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.13e-11 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 985 total aligned reads displayed.
CGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACC > CP009273/3462370‑3462504 | gcgcgAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGcc < 1:3603181/68‑1 (MQ=255) gcgcgAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGcc < 1:38359140/68‑1 (MQ=255) gcgcgAACATAACCCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGcc < 1:46857095/68‑1 (MQ=255) gcgAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCt < 1:29222669/67‑1 (MQ=255) gAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTAcc < 1:22927858/68‑1 (MQ=255) gAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTAc > 1:48534868/1‑67 (MQ=255) aaaCATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTAcc < 1:7099063/67‑1 (MQ=255) tcATAACGCCATGGGTTACTGGCAGCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTAccc < 1:10428228/66‑1 (MQ=255) aCATCACTCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTAccc < 1:14142364/67‑1 (MQ=255) aCATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACcca < 1:40763216/68‑1 (MQ=255) aCATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACcca < 1:5367076/68‑1 (MQ=255) aCATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACcca < 1:5855776/68‑1 (MQ=255) aTAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGAAGCGCCTACccacc > 1:49259258/1‑68 (MQ=255) aTAACGCCATGGGTTACTGGCATCGTTTACTTTCGCATCGCCAGGCCAATTTGCCGAGCCTAcccccc > 1:30548293/1‑68 (MQ=25) tAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACccacca < 1:10677090/68‑1 (MQ=255) aCGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGAAGCGGCTACCCACCAg > 1:39521951/1‑67 (MQ=25) cGCCATGGGTTACTGGCATCGTTTTATTTCGCATAGCCTGGGCAATTTGGAGAACCAACCCAACAgga > 1:25424010/1‑67 (MQ=19) gCCATGGGTTTCTGGCATCGTTTTCTTTCGCATCGCCAGGACAGTTTGAAGCGGCTACCCACCAGGca > 1:8701720/1‑67 (MQ=14) gCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCg > 1:49883696/1‑68 (MQ=255) ccATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGt > 1:25011391/1‑68 (MQ=255) cATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGtt < 1:7890339/68‑1 (MQ=255) cATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGt < 1:47821590/67‑1 (MQ=255) tGGGTTACTGGCATCTTTATCTCTCGCACCGCCTGGGCAATTTGCCGAGCCTACCCACCAGGCGTTCt < 1:42042452/68‑1 (MQ=255) tGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCt < 1:28438333/68‑1 (MQ=255) tGGGTTACTGGCATCGTTATCTATCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCt < 1:18347540/68‑1 (MQ=255) tGGGTTACTGGCATCGTCATCTATCGCAGCCCCTGGGCAATCTGACGCGCCAACCCACCAGGCGTTCt < 1:33592933/68‑1 (MQ=21) gggTTACTGGCTTAGTGTTCTATCGCAGCGCGTGGGCAATTTTCCGCGCCTACCCACCAGGCGTTCtt < 1:38843202/68‑1 (MQ=25) ggTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGAAGAGCCTACCCACCAGGCATTTTta > 1:48902933/1‑68 (MQ=18) ggTTACTGGCATCGTTTTCTTTCGCATCGCCGGGGCAATTAGAAGAGGCTACCCACCAGGCTTTTTta > 1:48165372/1‑68 (MQ=14) ggTTACCGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGttatta > 1:23937740/1‑68 (MQ=255) tACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATaa < 1:43139079/68‑1 (MQ=255) aCTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCTTTATtatata > 1:10006482/1‑66 (MQ=38) tGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTATTATaaaa > 1:8241272/1‑66 (MQ=255) ggCATCGTCTTCTATAGCTTCGCCTGGGCAATTTTCAGAGCCAACCCACCAGGCGTTCTTATAAAt < 1:16769808/66‑1 (MQ=12) tCGTTTTCTTTCGCATCGCCTGGGCAATTTTCCGCGCCTACCCACCAGGCGTTCTTATAAATgccgcc < 1:35715004/68‑1 (MQ=255) tCGTTTTCTTTCGCATCGCCTGGGCAATTTTCCGCGCCTACCCACCAGGCGTTCTTATAAATgccgcc < 1:23326747/68‑1 (MQ=255) tCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATgccgcc < 1:1806493/68‑1 (MQ=255) tCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATgccgcc < 1:20216482/68‑1 (MQ=255) cGTTTTCTTTCGCATCGCCTGTGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGccgccg > 1:14643378/1‑68 (MQ=255) tttttCTTTCGCATCGCCTGGGGAATTTTACGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:4038074/67‑1 (MQ=38) tttttCTATCGTACCGCCTGGGCAATTTGACGAGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:40015013/67‑1 (MQ=37) gtcttctttcGCAACGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:45044283/65‑1 (MQ=255) gTTTTCTTTCGGATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:26286795/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:9531451/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:3515583/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:3454604/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:7549203/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:51209532/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:42402915/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:42839142/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:47244896/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:4354798/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:1533473/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:22585951/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:24297630/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:16999613/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:28076333/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:16402679/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:2991699/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:1348244/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:12836852/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:30899750/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:31873960/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:11580668/68‑1 (MQ=255) gTTATCTTTCGCATCGCCTGGGCAATTTTACGCGCCAACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:17580224/68‑1 (MQ=37) ttctttcGCATCGCGTGGGCAATTTGGCGAGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTtat < 1:19263393/68‑1 (MQ=38) ttctttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTtat < 1:37388600/68‑1 (MQ=255) ttctttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTtat < 1:31381728/68‑1 (MQ=255) ttctttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTtat < 1:43960686/68‑1 (MQ=255) tctttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTtata > 1:33013456/1‑68 (MQ=255) ctttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAg > 1:19550438/1‑68 (MQ=255) tttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGa > 1:32758633/1‑68 (MQ=255) ttcGCATCGGCTGGGCAATTTGCCGCGCCTTCCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:36759635/68‑1 (MQ=255) ttcGCATCGCCTGGGCAATTTGGCGCGCCAACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:27747961/68‑1 (MQ=255) ttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTGTTATAAATCCCGCCTTTATAGAt > 1:44445396/1‑68 (MQ=255) ttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAGATGCCGCCGTTATAGAt < 1:6508585/68‑1 (MQ=255) ttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt > 1:26907884/1‑68 (MQ=255) ttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:12415152/68‑1 (MQ=255) ttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:21574594/68‑1 (MQ=255) ttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:29596762/68‑1 (MQ=255) atcGCATCGCCTGGGCAATCTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:22186034/67‑1 (MQ=255) gCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAAc < 1:11119744/68‑1 (MQ=255) gCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAAc < 1:27104150/68‑1 (MQ=255) cATCGCGTGGGCAATTTTCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAAc < 1:50341294/67‑1 (MQ=255) aTCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGt < 1:37843626/68‑1 (MQ=255) aTCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGt < 1:15879/68‑1 (MQ=255) aTCGCCTGGGCAATTTGACGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGt < 1:32423380/68‑1 (MQ=255) ccTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCa > 1:21055324/1‑68 (MQ=255) cTGGGCAATTTTCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:46341279/68‑1 (MQ=255) cTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:20648492/68‑1 (MQ=255) cTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:18649446/68‑1 (MQ=255) cTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:36373408/68‑1 (MQ=255) gCAATTTTCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCTTTATAGATAACGTACCATGTa < 1:41575765/67‑1 (MQ=255) gCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTag > 1:12160667/1‑68 (MQ=255) gCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTag > 1:2595895/1‑68 (MQ=255) gCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTa < 1:28650512/67‑1 (MQ=255) gCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTa < 1:27394911/67‑1 (MQ=255) aaTTTGCCGCGCCTACCCACCAGGCGTTGTTATATAGCCCCCCGTTAAAGAGGACCTACCAGGTacta > 1:41185370/1‑65 (MQ=9) ttGCCGCGCCTACCCACCAGGCGTTGTTATAAATGACGCCGTAATAGATAACGTCCCATGAagaaga > 1:45477384/1‑67 (MQ=255) tGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAAc < 1:50929972/68‑1 (MQ=255) | CGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACC > CP009273/3462370‑3462504 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |