| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,462,438 | C→A | 9.8% | A259S (GCA→TCA) ‡ | chiA ← | periplasmic endochitinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,462,438 | 0 | C | A | 9.8% | 2762.9 / 47.9 | 983 | A259S (GCA→TCA) ‡ | chiA | periplasmic endochitinase |
| Reads supporting (aligned to +/- strand): ref base C (221/631); new base A (39/60); total (261/722) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.99e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 984 total aligned reads displayed.
GCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCT > CP009273/3462374‑3462505 | gcgAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCt < 1:48938882/67‑1 (MQ=255) gAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTAcc < 1:22927858/68‑1 (MQ=255) tcATAACGCCATGGGTTACTGGCAGCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTAccc < 1:10428228/66‑1 (MQ=255) aCATCACTCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTAccc < 1:14142364/67‑1 (MQ=255) aTAACGCCATGGGTTACTGGCATCGTTTACTTTCGCATCGCCAGGCCAATTTGCCGAGCCTAcccccc > 1:30548293/1‑68 (MQ=25) tAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACccacca < 1:10677090/68‑1 (MQ=255) aCGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAg > 1:46639509/1‑67 (MQ=255) gCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCAGCGCCTACCCACCAGGCg > 1:25411174/1‑68 (MQ=255) ccATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGt > 1:5034379/1‑68 (MQ=255) ccATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGt > 1:16392032/1‑68 (MQ=255) ccATGGGTTACTGGCATCGTTTTATTTCGCATCGCCAGGCCAATTTGAAGCGGCTACCCACCAGGcat > 1:8249430/1‑66 (MQ=17) cATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGtt < 1:40797089/68‑1 (MQ=255) cATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGtt < 1:31906331/68‑1 (MQ=255) aTGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTc < 1:35163275/68‑1 (MQ=255) tGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCt < 1:19241276/68‑1 (MQ=255) tGGGTTACTGGCATCGTTATCTATCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCt < 1:18347540/68‑1 (MQ=255) tGGGTTACTGGCATCGTCATCTATCGCAGCCCCTGGGCAATCTGACGCGCCAACCCACCAGGCGTTCt < 1:33592933/68‑1 (MQ=21) tGGGTTACTGGCATCGTATTCTATCGCATCGCCTGGGCAATTTTCCGCGCCTACCCACCAGGCGTTCt < 1:7962534/68‑1 (MQ=255) gggTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCATTTTGCCGCGCCTACCCACCAGGCtttttt > 1:18607345/1‑68 (MQ=38) ggTTACTGGCATCGTTTTCTTTCGCATCGCCAGGGCACTTTGCCGCGCCTACCCACCAGGCGttatta > 1:29268966/1‑68 (MQ=38) ggTTACCGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGttatta > 1:23937740/1‑68 (MQ=255) gTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGAAGCGCCTACCCACCAGGCATTTTtat > 1:2865745/1‑68 (MQ=21) gTTACTGGCATCGTTTTCTTTCGCATCGCCTGGCCAATTTGCAGCGCCTACCCACCAGGCATTTTtat > 1:42454306/1‑68 (MQ=25) ttACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTtata > 1:42876126/1‑68 (MQ=255) ttACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGAAGCGGCTACCCACCAGGCGTTTTtata > 1:48198432/1‑68 (MQ=21) aCTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCTTTATtatata > 1:10006482/1‑66 (MQ=38) tGGCATCGTTTTCTTTCGCATCGCCTGGGCAGTTTGCCGCGCCTACCCACCAGGCGTTATTATaaaa > 1:47860772/1‑66 (MQ=38) tGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTATTATaaaac > 1:3638786/1‑66 (MQ=255) ggCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATg > 1:7168998/1‑67 (MQ=255) ggCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTATTATaaagc > 1:3604352/1‑65 (MQ=255) ggCATCGTTTTATTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTATTATAAATg > 1:31429157/1‑67 (MQ=255) ggCATCGTCTTCTATAGCTTCGCCTGGGCAATTTTCAGAGCCAACCCACCAGGCGTTCTTATAAAt < 1:16769808/66‑1 (MQ=12) tCGTTTTCTTTCGCATCGCCTGGGCAATTTTCCGCGCCTACCCACCAGGCGTTCTTATAAATgccgcc < 1:23326747/68‑1 (MQ=255) tCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATgccgcc < 1:1806493/68‑1 (MQ=255) tCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATgccgcc < 1:20216482/68‑1 (MQ=255) cGTTTTCTTTCGCATCGCCTGTGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGccgccg > 1:14643378/1‑68 (MQ=255) ttgttctttgGCATCGCGTGGGCAATATTCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:35726304/65‑1 (MQ=37) gTTTTCTTTCGGATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:26286795/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAATTGCCGCCGt < 1:27165307/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:9531451/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:34592415/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:39669502/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:45046612/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:47492945/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:48593580/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:6659790/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:7608963/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:2991699/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:16999613/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:22585951/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:24297630/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:24913810/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:1533473/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:1348244/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:12836852/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:11580668/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:28076333/68‑1 (MQ=255) gTTTTCTTTCGCAGCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:45633387/68‑1 (MQ=255) gTTTGCTATCGCATCCCCTGGGCAATCTTACGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:50982306/68‑1 (MQ=25) gTTATCTTTCGCATCGCCTGGGCAATTTTACGCGCCAACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:17580224/68‑1 (MQ=37) ttttATTTCCCAGCGCGTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:871365/67‑1 (MQ=38) tgttctttcGCATCGCCTGGGCAATTTGACGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:44613628/65‑1 (MQ=255) gtgctttcGCATCCCCTGGGCAATTTTCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:30917557/63‑1 (MQ=38) ttctttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTtat < 1:50053351/68‑1 (MQ=255) ttctttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTtat < 1:22167681/68‑1 (MQ=255) tctttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTtata > 1:33013456/1‑68 (MQ=255) ctttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAg > 1:19550438/1‑68 (MQ=255) tttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGa > 1:32758633/1‑68 (MQ=255) ttcGCATCGCCTGGGCAATTTGGCGCGCCAACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:27747961/68‑1 (MQ=255) ttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:43612318/68‑1 (MQ=255) ttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:21574594/68‑1 (MQ=255) ttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:40431147/68‑1 (MQ=255) ttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:38455685/68‑1 (MQ=255) ttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:12415152/68‑1 (MQ=255) ttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt > 1:26907884/1‑68 (MQ=255) ttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:29596762/68‑1 (MQ=255) atcGGATCGCCTGGGCAATTTGTCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:49350643/67‑1 (MQ=39) gCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAAc < 1:11119744/68‑1 (MQ=255) gCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAAc < 1:28468673/68‑1 (MQ=255) aTCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGt < 1:5954832/68‑1 (MQ=255) aTCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGt < 1:15879/68‑1 (MQ=255) aTCGCCTGGGCAATTTGACGAGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGt < 1:40110387/68‑1 (MQ=255) gCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTAcc < 1:5533599/68‑1 (MQ=255) gCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTAcc < 1:41231653/68‑1 (MQ=255) ccgtGGGCAATTTTCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTAcc < 1:44141331/65‑1 (MQ=255) ccTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCa > 1:21055324/1‑68 (MQ=255) cTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:32470636/68‑1 (MQ=255) cTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:3741311/68‑1 (MQ=255) cTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:37995687/68‑1 (MQ=255) cTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:43202201/68‑1 (MQ=255) cTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:20648492/68‑1 (MQ=255) cTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:46861510/68‑1 (MQ=255) cTGGGCAATTTGCCGCGCCTACCAACAAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:51243598/68‑1 (MQ=255) gggCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGt < 1:41608230/68‑1 (MQ=255) gCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTag > 1:2595895/1‑68 (MQ=255) gCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTag > 1:12160667/1‑68 (MQ=255) gCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTa < 1:27394911/67‑1 (MQ=255) gCAATCTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTa < 1:36777839/67‑1 (MQ=255) aaTTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTaga < 1:42062824/67‑1 (MQ=255) tttGACGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTagaaga < 1:38944586/68‑1 (MQ=255) | GCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCT > CP009273/3462374‑3462505 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |