| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,462,676 | A→T | 7.1% | A179A (GCT→GCA) | chiA ← | periplasmic endochitinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,462,676 | 0 | A | T | 7.1% | 2867.4 / 58.6 | 868 | A179A (GCT→GCA) | chiA | periplasmic endochitinase |
| Reads supporting (aligned to +/- strand): ref base A (443/347); new base T (9/67); total (453/415) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.96e-14 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 877 total aligned reads displayed.
CCGGTGAAGGCTGTGGTGCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAGTTAGAAG > CP009273/3462609‑3462743 | gAAGGCTGTGGCGCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGc < 1:29836077/68‑1 (MQ=255) aaGGCTGTGGTGCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGc > 1:16234872/1‑67 (MQ=255) aGGCTGTGGTGCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCAc > 1:36900633/1‑68 (MQ=255) gCTGTGGTGCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGa > 1:49215591/1‑68 (MQ=255) cTGTGGTGCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGa > 1:46222533/1‑67 (MQ=255) cTGTGGTGCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGa > 1:50182026/1‑67 (MQ=255) cTGTGGTGCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGa < 1:47943517/67‑1 (MQ=255) tgtgGTGCTGACGTACAGGACTGTGGATTACTGGTTTCTGAGATATCCGTTGCCGTAGCGGCACGaa < 1:39491119/67‑1 (MQ=255) tgtgGTGCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAAc < 1:34330379/68‑1 (MQ=255) tgtgGGGCTGACGTACAGGACCGTGGTTTACTGGTTTCTGTGATTTCCGTTGCCGTAGCGGCACGAAc < 1:17470792/68‑1 (MQ=255) tgcggtgCAGAAGAACAGGACTGTGGATTACAGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAAc < 1:19970128/65‑1 (MQ=255) tgCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAAc > 1:41752801/1‑68 (MQ=255) tgCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAAc > 1:37574063/1‑68 (MQ=255) gCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAAc > 1:12801645/1‑67 (MQ=255) gCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACg < 1:47109190/68‑1 (MQ=255) aGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATg > 1:46761334/1‑68 (MQ=255) aGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATg > 1:40905615/1‑68 (MQ=255) aGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATg > 1:40512081/1‑68 (MQ=255) aGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATg > 1:21194726/1‑68 (MQ=255) tACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATggg > 1:34630193/1‑68 (MQ=255) aCAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATggg < 1:8276588/67‑1 (MQ=255) aCAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATgg < 1:9696614/66‑1 (MQ=255) aCAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATgg < 1:314263/66‑1 (MQ=255) aCAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGt < 1:32279498/68‑1 (MQ=255) aCAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGt > 1:27528486/1‑68 (MQ=255) aGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGtt > 1:43326512/1‑67 (MQ=255) aGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGtt > 1:21979196/1‑67 (MQ=255) aGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGtt > 1:48587836/1‑67 (MQ=255) aGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGtt > 1:4145298/1‑67 (MQ=255) aGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGtt > 1:28066855/1‑67 (MQ=255) gACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGtt > 1:14578838/1‑65 (MQ=255) aCTGTGGATTACTGTTTTCTGAGATTTCCGTTGCCGTAGCGTAACAAACATAACACCATCGGTTGCTg > 1:7462344/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:31756393/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:44259776/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:42491680/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:3982827/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:4575097/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:1567027/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:17862537/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:6091152/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:5445441/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:50455260/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:22190167/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:24960737/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:2549482/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTCCTg > 1:13372774/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCAGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:11755235/1‑68 (MQ=255) aCTGTGGAAAACTGGTTTCTGAGATTTCAGTTGCCGTAGCGGCACGAACATAACCCCATGGGTTGCTg > 1:14220759/1‑68 (MQ=255) tgtgGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGc < 1:29080001/68‑1 (MQ=255) ggATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGcatc > 1:44819125/1‑68 (MQ=255) ggATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGcatc > 1:38992292/1‑68 (MQ=255) ggATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGcatc > 1:18212535/1‑68 (MQ=255) ggATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCCGGcatc > 1:12060469/1‑68 (MQ=255) aCTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCAtt > 1:4397373/1‑66 (MQ=255) aCTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCAtt > 1:69406/1‑66 (MQ=255) tGGTTTCAGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCtt > 1:6532615/1‑68 (MQ=255) ggTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCttt > 1:24537031/1‑68 (MQ=255) tCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGc < 1:38152935/68‑1 (MQ=255) gagaTTTCCGTTGCCGTGGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATc > 1:26917027/1‑68 (MQ=255) tgaTTTCTGTTGCCGTTGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCa < 1:36170061/67‑1 (MQ=18) gaTTTCTGTTGCCGTAGCGGCTCGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCa < 1:15225869/67‑1 (MQ=255) gaTTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCa < 1:42633200/67‑1 (MQ=255) gaTTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCa < 1:9319245/67‑1 (MQ=255) gaTTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCa < 1:33620832/67‑1 (MQ=255) gaTTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCa < 1:35240920/67‑1 (MQ=255) gaTTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCa < 1:32700719/67‑1 (MQ=255) gaTTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCa > 1:2327779/1‑67 (MQ=255) gaTTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCa < 1:2855386/67‑1 (MQ=255) gaTTTCCGGTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCa < 1:51004844/67‑1 (MQ=255) gaTTTCAGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCa < 1:4530306/67‑1 (MQ=255) tttCTGTTGCCGTTGCGGCTCGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCAcc < 1:10825751/67‑1 (MQ=18) tttCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCAcc < 1:14881097/67‑1 (MQ=255) tttCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCAcc < 1:18752650/67‑1 (MQ=255) tttCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCAcc < 1:43007250/67‑1 (MQ=255) tttCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCAcc < 1:26568705/67‑1 (MQ=255) ttCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCg < 1:2596538/68‑1 (MQ=255) tCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCgg < 1:19025701/68‑1 (MQ=255) ctgTTGCCGTTGCGGCTCGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGa < 1:33994768/66‑1 (MQ=21) gTTGCCGTAGCGGCACGAACATAACTCCATGGGTTGCTGGCATCATTGCTTTTCGCATCA‑CCGGACAg < 1:35642527/68‑1 (MQ=255) tGCCGTTGCGGCTCGATCATATCGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGTACAg < 1:47410854/67‑1 (MQ=255) tGCCGTTGCGGCTCGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAg < 1:12398766/67‑1 (MQ=255) tGCCGTTGCGGCTCGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAg < 1:45987879/67‑1 (MQ=255) tGCCGTTGCGGCTCGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAGt < 1:30498997/68‑1 (MQ=255) tGCCGTTGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAGt < 1:1015306/68‑1 (MQ=255) tGCCGTAGCTGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAg < 1:23825531/67‑1 (MQ=255) tGCCGTAGCGGCTCGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAg < 1:33180617/67‑1 (MQ=255) tGCCGTAGCGGCTCGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAGt < 1:49696277/68‑1 (MQ=255) tGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAg < 1:11350153/67‑1 (MQ=255) tGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAg < 1:7954880/67‑1 (MQ=255) tGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAg < 1:19455782/67‑1 (MQ=255) tGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAg < 1:43796435/67‑1 (MQ=255) tGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAg < 1:18479530/67‑1 (MQ=255) tGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAGt < 1:30922204/68‑1 (MQ=255) tGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAGt < 1:22434520/68‑1 (MQ=255) tGCCGTAGCGGCACGAACATAACGCCATGGGGTGCTGGCATCATTGCTTTTCGCATCACCCGGACAg < 1:21624936/67‑1 (MQ=255) gCCGTTGCGGCTCGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAGtt < 1:37281003/68‑1 (MQ=255) gCCGTAGCGGCTCGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCTGACAGtt < 1:22878430/68‑1 (MQ=255) gCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAGtt < 1:20518218/68‑1 (MQ=255) gTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAGTTaga < 1:8636632/68‑1 (MQ=255) aGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAGTTagaag > 1:16933375/1‑68 (MQ=255) | CCGGTGAAGGCTGTGGTGCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAGTTAGAAG > CP009273/3462609‑3462743 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |