| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 122,524 | A→G | 6.1% | S115G (AGC→GGC) | aceF → | pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 122,524 | 0 | A | G | 6.1% | 555.8 / 9.3 | 197 | S115G (AGC→GGC) | aceF | pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2 |
| Reads supporting (aligned to +/- strand): ref base A (156/23); new base G (7/5); total (166/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.80e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 218 total aligned reads displayed.
AGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGC > CP009273/122457‑122590 | gaagCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCa > 1:43867696/1‑67 (MQ=255) gaagCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCa > 1:31339489/1‑67 (MQ=255) gaagCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCa > 1:39107424/1‑67 (MQ=255) gaagCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCa > 1:17069613/1‑67 (MQ=255) gcagcTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTTACGTTGCGGATATCGGCAGcg > 1:41379604/1‑67 (MQ=255) gcagcTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGgcggcg > 1:15831800/1‑67 (MQ=255) gcagcTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGcg > 1:32628729/1‑67 (MQ=255) gcagcTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGcg > 1:2468093/1‑67 (MQ=255) gcagcTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGcg > 1:12185678/1‑67 (MQ=255) gcagcTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGcg > 1:24148755/1‑67 (MQ=255) gcagcTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGcg > 1:23074621/1‑67 (MQ=255) gcagcTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGcg > 1:17242264/1‑67 (MQ=255) gcagcTCCGGCAGCAGCACCAGCGCCTGCGGCTGCAAATGACGTTAAACTACCGCATATCGGCACcg > 1:34891219/1‑67 (MQ=25) cTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCGGCGACGaa > 1:1444554/1‑68 (MQ=255) tCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATTTCGGCAGCGACGAAg > 1:6279269/1‑68 (MQ=255) tCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCCGCGACGAAg > 1:7653411/1‑68 (MQ=255) tCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGaa < 1:3101917/67‑1 (MQ=255) tCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAg > 1:37005157/1‑68 (MQ=255) tCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAg > 1:26085680/1‑68 (MQ=255) tCCGGCAGCAGCACCAGCGGCTGAGGCGGCAATAGACGTTAACGTTCCGGATATCGGCTGAGACGAAg > 1:27588307/1‑68 (MQ=25) ccGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGt < 1:30355766/68‑1 (MQ=255) cagcagcaCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCGGCGACGAAGTTGaa > 1:23969576/1‑68 (MQ=255) cagcagcaCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCCGGGACGAAGTTGaa > 1:2267675/1‑68 (MQ=255) cagcagcaCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGaa > 1:5312305/1‑68 (MQ=255) cagcagcaCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGaa > 1:1519702/1‑68 (MQ=255) cagcagcaCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGaa > 1:36175580/1‑68 (MQ=255) cagcagcaCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGaa > 1:45514685/1‑68 (MQ=255) cagcagcaCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGAAGATGTTGaa > 1:32791835/1‑68 (MQ=255) cagcagcaCCAGCGGCTGCGGCGGCAAAAGACGGTAACGTTGCGGATATCGGCAGCGACGATGTTGaa > 1:7567672/1‑68 (MQ=38) agcagcaCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAg > 1:20802618/1‑68 (MQ=255) agcagcaCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAg > 1:36420168/1‑68 (MQ=255) gcagcaCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCGGGGACGATGTTGATGt > 1:11592474/1‑68 (MQ=21) gcagcaCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGt > 1:4010457/1‑68 (MQ=255) gcagcaCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGt > 1:38815234/1‑68 (MQ=255) gcagcaCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGt > 1:24286842/1‑68 (MQ=255) cctgcaCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGt > 1:22847553/4‑68 (MQ=255) agcaCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTg > 1:29701609/1‑67 (MQ=255) caCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGAcc > 1:8100665/1‑68 (MQ=255) aCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGAc < 1:10205144/66‑1 (MQ=255) aCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGAc < 1:16168165/66‑1 (MQ=255) ccAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGa > 1:20581092/1‑68 (MQ=255) cGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACGGAAAtg > 1:45434718/1‑67 (MQ=255) cGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAAt > 1:13837292/1‑67 (MQ=255) cGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAAt > 1:39611640/1‑67 (MQ=255) cGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAAt > 1:16710101/1‑67 (MQ=255) cGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCAGCGGCGACGAAGTTGAAGTGACAGaatt > 1:5452327/1‑65 (MQ=18) ggCTGCGGCGGCAAAAGACGTTAGCGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAAt > 1:27876543/1‑66 (MQ=255) ggCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAAt > 1:37525832/1‑66 (MQ=255) tGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTgg > 1:4224103/1‑68 (MQ=255) cGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATa > 1:44229845/1‑68 (MQ=255) cGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATa > 1:37752991/1‑68 (MQ=255) cGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATa > 1:8620605/1‑68 (MQ=255) cGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACAGAACTCCTGGTGAAAGTTGGCGATa > 1:676839/1‑68 (MQ=255) aCGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAATTCCTGGTGAAAGTTGGCGATAAAGtt > 1:46511851/1‑68 (MQ=255) aCGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGtt > 1:7190514/1‑68 (MQ=255) aCGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGtt > 1:10452028/1‑68 (MQ=255) aCGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGtt > 1:9331996/1‑68 (MQ=255) aCGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGtt > 1:46408357/1‑68 (MQ=255) aCGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGtt > 1:2478388/1‑68 (MQ=255) aCGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACAGAAATCCTGGTGAAAGTTGGCGATAAAGtt > 1:41971512/1‑68 (MQ=255) aCGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACAGAAATCATGGTGAAAGTGGGCGAGAAAGtt > 1:38372296/1‑68 (MQ=14) cGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTg > 1:34683887/1‑68 (MQ=255) cGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTg > 1:37414808/1‑68 (MQ=255) cGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTg > 1:45109872/1‑68 (MQ=255) gTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGa > 1:2005884/1‑68 (MQ=255) gTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGa > 1:43508954/1‑68 (MQ=255) gTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACAGAAATCATGGTGAAAGTTGGCGATAAAGTTGa > 1:28966651/1‑68 (MQ=255) ttCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAACTCCTGGTGAAAGTTGGCGATAAAGTTGaa > 1:3387789/1‑68 (MQ=255) tCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGaa < 1:11692254/67‑1 (MQ=255) tCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGaa < 1:48442101/67‑1 (MQ=255) tCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGaa < 1:24402215/67‑1 (MQ=255) tCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGaa < 1:34383295/67‑1 (MQ=255) tCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGaa > 1:4067545/1‑67 (MQ=255) accGGACATCGGGGCCGACGAAGTTGAACTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGaa < 1:20190809/66‑1 (MQ=14) cGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGaa > 1:41916613/1‑65 (MQ=255) atatCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGa > 1:18014055/1‑67 (MQ=255) atatCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGa > 1:17260434/1‑67 (MQ=255) atatCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGa > 1:38054682/1‑67 (MQ=255) atatCGGCAGCGACGAAGTTGAAGTGACCGAAATCATTATGAAAGTTGGCGACAAAGTTGAAGCCGa > 1:48039179/1‑67 (MQ=18) atatCGGCAGCGACGAAGTTGAAGTGACCGAAATCATGGTGAAAGTTGGCGGAAAAGTTGACGCTGa > 1:49839704/1‑67 (MQ=18) atatCGGCAGCGACGAAGTTGAAGTGACAGAAATCCTGGTGAAAGTTGGCGAGAAAGTTGACGCTGa > 1:34638635/1‑67 (MQ=18) tatCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAAc > 1:6720599/1‑68 (MQ=255) tatCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAAc > 1:25722704/1‑68 (MQ=255) tCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAg > 1:20518378/1‑68 (MQ=255) tCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAg > 1:34050945/1‑68 (MQ=255) tCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAg > 1:20575242/1‑68 (MQ=255) cGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGt < 1:27500779/68‑1 (MQ=255) ggCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTc < 1:45136882/68‑1 (MQ=255) ggCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTc < 1:43147824/68‑1 (MQ=255) ggCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTc < 1:3071147/68‑1 (MQ=255) ggCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTc < 1:35991062/68‑1 (MQ=255) gCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTc > 1:50524339/1‑67 (MQ=255) gCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTc > 1:21401867/1‑67 (MQ=255) gCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTc > 1:47722109/1‑67 (MQ=255) gCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTc > 1:27110815/1‑67 (MQ=255) gCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTc > 1:16840124/1‑67 (MQ=255) gCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGAAAAAGTTGAAGCTGAACAGTc > 1:43630394/1‑67 (MQ=255) aGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGc < 1:32199425/67‑1 (MQ=255) aGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGc < 1:40583405/67‑1 (MQ=255) aGCGACGAAGTAGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTCAACAGTc > 1:18500745/1‑65 (MQ=255) | AGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGC > CP009273/122457‑122590 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |