BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,106,987	G→A	11.4%	A55V (GCC→GTC) ‡	glpK ←	glycerol kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,106,987	0	G	A	11.4%	2157.8 / 210.3	737	A55V (GCC→GTC) ‡	glpK	glycerol kinase
Reads supporting (aligned to +/- strand): ref base G (290/359); new base A (43/41); total (335/402)
Fisher's exact test for biased strand distribution p-value = 2.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 769 total aligned reads displayed.

AGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCT  >  CP009273/4106920‑4107053

 gCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTgg                                                                    >  1:10745671/1‑67 (MQ=255)

 gCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTgg                                                                    >  1:31352026/1‑67 (MQ=255)

      aaTTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGa                                                              <  1:8318579/68‑1 (MQ=255)

      aaTTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGa                                                              <  1:11603347/68‑1 (MQ=255)

      aaTTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGa                                                              <  1:4803000/68‑1 (MQ=255)

      aaTTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGa                                                              <  1:1391524/68‑1 (MQ=255)

      aaTTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGACCCAGa                                                              <  1:18069321/68‑1 (MQ=255)

        tttGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGTCCAGAtt                                                            >  1:28679174/1‑68 (MQ=255)

        tttGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGTCCAGAtt                                                            <  1:22159557/68‑1 (MQ=255)

        tttGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGAtt                                                            >  1:38087953/1‑68 (MQ=255)

        tttGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGACCCAGAtt                                                            <  1:33143015/68‑1 (MQ=255)

         ttGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTA‑CAGCGTGGAGCTTTGGGTGGCCCAGAttt                                                           >  1:33484211/1‑67 (MQ=255)

           gATCGGAACTGATTTCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTcc                                                         <  1:43813232/68‑1 (MQ=255)

           gATCGGAACTGATCTCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTcc                                                         <  1:15901825/68‑1 (MQ=255)

           gATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTcc                                                         <  1:9076234/68‑1 (MQ=255)

           gATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTcc                                                         <  1:41350193/68‑1 (MQ=255)

           gATCGGAACTGATATCGGCTTTCACCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTcc                                                         >  1:27542949/1‑68 (MQ=255)

            aTCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCa                                                        >  1:47163162/1‑68 (MQ=255)

             tCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGACCCAGATTTCCAt                                                       >  1:31690675/1‑68 (MQ=255)

                 aaCTGATATCGGCTTTCGTCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTgg                                                    <  1:32892871/67‑1 (MQ=255)

                 aaCTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTgg                                                    <  1:7415486/67‑1 (MQ=255)

                  aCTGATATCGGCTTTCGTCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTggg                                                   >  1:45380700/1‑67 (MQ=255)

                  aCTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGTCCAGATTTCCATTGGGt                                                  <  1:48800239/68‑1 (MQ=255)

                  aCTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTggg                                                   >  1:40458176/1‑67 (MQ=255)

                  aCTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTggg                                                   >  1:18815789/1‑67 (MQ=255)

                  aCTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTggg                                                   >  1:42934479/1‑67 (MQ=255)

                  aCTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTggg                                                   >  1:12433840/1‑67 (MQ=255)

                  aCTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGt                                                  <  1:41769049/68‑1 (MQ=255)

                  aCTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGt                                                  <  1:29548949/68‑1 (MQ=255)

                  aCTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGt                                                  <  1:3989831/68‑1 (MQ=255)

                  aCTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGt                                                  <  1:30202532/68‑1 (MQ=255)

                  aCTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGACCCAGATTTCCATTGGGt                                                  <  1:19241542/68‑1 (MQ=255)

                     gATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgt                                               <  1:47484025/68‑1 (MQ=255)

                       tctCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtg                                              <  1:26776511/65‑1 (MQ=255)

                       tatCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGTCCAGATTTCCATTGGGTCgtgt                                             >  1:49871382/1‑68 (MQ=255)

                       tatCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtgt                                             <  1:11393530/68‑1 (MQ=255)

                       tatCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtgt                                             <  1:38506096/68‑1 (MQ=255)

                       tatCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtgt                                             <  1:42477086/68‑1 (MQ=255)

                       tatCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtgt                                             <  1:369644/68‑1 (MQ=255)

                       tatCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtgt                                             >  1:35335459/1‑68 (MQ=255)

                       tatCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtgt                                             >  1:28101538/1‑68 (MQ=255)

                       tatCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtgt                                             >  1:20099448/1‑68 (MQ=255)

                       tatCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtgt                                             >  1:20428945/1‑68 (MQ=255)

                       tatCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtgt                                             >  1:6063700/1‑68 (MQ=255)

                       tatCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtgt                                             <  1:16200309/68‑1 (MQ=255)

                       tatCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtgt                                             >  1:22932692/1‑68 (MQ=255)

                       tatCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtgt                                             >  1:49347467/1‑68 (MQ=255)

                       tatCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtgt                                             >  1:24589917/1‑68 (MQ=255)

                       tatCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtgt                                             <  1:25797804/68‑1 (MQ=255)

                       tatCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtgt                                             <  1:28317400/68‑1 (MQ=255)

                       tatCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtgt                                             <  1:46142233/68‑1 (MQ=255)

                       tatCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGACCCAGATTTCCATTGGGTCgtg                                              <  1:29217674/67‑1 (MQ=255)

                       tatCGGCTTTCGCCAGCACTCCTACCCGCGTGGAGCGTTGTGTGGCCCAGATTTCCATTGGGTCgtgt                                             <  1:34864804/68‑1 (MQ=255)

                            gCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTa                                         >  1:39428729/1‑67 (MQ=255)

                            gCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTa                                         >  1:37663934/1‑67 (MQ=255)

                            gCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTa                                         >  1:34114701/1‑67 (MQ=255)

                            gCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTa                                         >  1:32513449/1‑67 (MQ=255)

                                 cgtcAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAAc                                   <  1:43940107/65‑1 (MQ=255)

                                 cGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAAc                                   <  1:35640845/68‑1 (MQ=255)

                                 cGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAAc                                   <  1:7754106/68‑1 (MQ=255)

                                 cGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAAc                                   <  1:15046339/68‑1 (MQ=255)

                                    cAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCt                                 <  1:26293460/67‑1 (MQ=255)

                                      gCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGt                              >  1:18445052/1‑68 (MQ=255)

                                        aCTTCTACCAGCGTGGGGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGt                              <  1:33792974/66‑1 (MQ=255)

                                        aCTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTgg                               <  1:1654247/65‑1 (MQ=255)

                                        aCTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGttt                            >  1:23593078/1‑68 (MQ=255)

                                        aCTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGttt                            >  1:6778024/1‑68 (MQ=255)

                                        aCTTCTACCAGCGTGGAGCTTTGGGTGACCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTgg                               <  1:3076555/65‑1 (MQ=255)

                                           tCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTgg                         <  1:10104869/68‑1 (MQ=255)

                                           tCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTgg                         <  1:50577452/68‑1 (MQ=255)

                                           tCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTgg                         <  1:8732631/68‑1 (MQ=255)

                                           tCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTgg                         <  1:14256016/68‑1 (MQ=255)

                                              aCCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGATCTACCCAACCTGGTTTTGGGTa                      >  1:45092960/1‑68 (MQ=255)

                                               ccAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAg                     >  1:5387896/1‑68 (MQ=255)

                                               ccAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAg                     >  1:30462958/1‑68 (MQ=255)

                                               ccAGCGTGGAGCTTTGCGTGGCCCAGAAATCGATTGGGTCGTGTTATACCCAACCAGGTTTTGGGTAg                     >  1:15618605/1‑68 (MQ=255)

                                                cAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGa                    >  1:39037046/1‑68 (MQ=255)

                                                cAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGa                    >  1:23370912/1‑68 (MQ=255)

                                                cAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGa                    >  1:19506326/1‑68 (MQ=255)

                                                 aGCGTGGGGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTCGTTTTGGGTAGAt                   >  1:48982898/1‑68 (MQ=255)

                                                   cGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGAttt                 >  1:41040100/1‑68 (MQ=255)

                                                   cGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGAttt                 >  1:21858751/1‑68 (MQ=255)

                                                   cGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGAttt                 >  1:50975500/1‑68 (MQ=255)

                                                   cGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAAACGGGTTTAGGGTAGAttt                 >  1:31038828/1‑68 (MQ=255)

                                                   cGTGGAGCTTTGGGTGACCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGAttt                 >  1:2701602/1‑68 (MQ=255)

                                                      ggAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGc               <  1:17021585/67‑1 (MQ=255)

                                                      ggAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGc               <  1:44617866/67‑1 (MQ=255)

                                                      ggAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGc               <  1:40691762/67‑1 (MQ=255)

                                                      ggAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGc               <  1:6567671/67‑1 (MQ=255)

                                                      ggAGCTTTGGGGGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGc               <  1:14765951/67‑1 (MQ=255)

                                                         gCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCaa           >  1:13188398/1‑68 (MQ=255)

                                                         gCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCaa           >  1:24072220/1‑68 (MQ=255)

                                                           tttGGGTGGCCCAGATTTCCATTGGGTCCTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAAt         >  1:21465551/1‑68 (MQ=255)

                                                               ggTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTcgc     >  1:11956585/1‑68 (MQ=255)

                                                                gTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTcgc     >  1:6347389/1‑67 (MQ=255)

                                                                gTGACCCAGATTTCCATTGGGTCGTGTTCTACCCAACCGGGTTTTGGGTAGATTTGCTCAAATTcgc     >  1:5608595/1‑67 (MQ=255)

                                                                 tGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTcgcg    <  1:20892611/67‑1 (MQ=255)

                                                                 tGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTcgcg    <  1:36295797/67‑1 (MQ=255)

                                                                  ggCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCt  <  1:25165289/68‑1 (MQ=255)

                                                                  ggCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGGTTTGGGTAGATTTGCTCAAATTCGCGCt  <  1:12809280/68‑1 (MQ=255)

AGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCT  >  CP009273/4106920‑4107053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`