| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 662,486 | G→T | 7.6% | A396A (GCC→GCA) | mrdA ← | transpeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 662,486 | 0 | G | T | 7.6% | 1568.8 / 18.9 | 478 | A396A (GCC→GCA) | mrdA | transpeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2) |
| Reads supporting (aligned to +/- strand): ref base G (100/336); new base T (39/3); total (139/339) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.70e-20 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 516 total aligned reads displayed.
TACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGC > CP009273/662419‑662553 | aCCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAgg < 1:17328928/68‑1 (MQ=255) aCCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAg > 1:29195784/1‑67 (MQ=255) ccGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAgg > 1:29914347/1‑67 (MQ=255) ccGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAgg > 1:30919368/1‑67 (MQ=255) ccGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAgg > 1:5906426/1‑67 (MQ=255) cGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGcc < 1:10548995/68‑1 (MQ=255) cGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGcc < 1:14085834/68‑1 (MQ=255) tgtAATGTCCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCTATCCCCATATCGTAGGCCAc < 1:7784636/67‑1 (MQ=255) tgtAATGGCCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCAc < 1:27181647/67‑1 (MQ=255) tgtAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTATGccg > 1:45881561/1‑65 (MQ=255) tgtAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCa > 1:3480404/1‑66 (MQ=255) tgtAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCa > 1:44582703/1‑66 (MQ=255) tgtAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCAc < 1:32453927/67‑1 (MQ=255) tgtAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCAc < 1:49643320/67‑1 (MQ=255) tgtAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCAc < 1:23877673/67‑1 (MQ=255) gtAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCAcc < 1:43289515/67‑1 (MQ=255) gtAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCAcc < 1:35533761/67‑1 (MQ=255) gtAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCAcc < 1:38909893/67‑1 (MQ=255) gtAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCAcc < 1:13659651/67‑1 (MQ=255) gtAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCAcc < 1:39856617/67‑1 (MQ=255) gtAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCAcc < 1:48412752/67‑1 (MQ=255) gtAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCAcc < 1:8290575/67‑1 (MQ=255) gtAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCAcc < 1:15333077/67‑1 (MQ=255) aaTGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGGTCGATCCCCATATCGTAGGCCAcccg < 1:26887340/67‑3 (MQ=255) aaTGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATCTCGTATGCCGCCTGa > 1:38745681/1‑68 (MQ=255) aaTGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCGCCTGa > 1:22328468/1‑68 (MQ=255) aaTGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCtta > 1:47789212/1‑66 (MQ=255) aaTGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTg < 1:43051925/67‑1 (MQ=255) aaTGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTg < 1:27404507/67‑1 (MQ=255) aaTGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTg < 1:16735218/67‑1 (MQ=255) aaTGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTg < 1:13360192/67‑1 (MQ=255) aaTGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGa < 1:39464012/68‑1 (MQ=255) aaTGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGa < 1:965332/68‑1 (MQ=255) aaTGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGa < 1:31627739/68‑1 (MQ=255) aaTGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGa < 1:25543222/68‑1 (MQ=255) aaTGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGa > 1:49082969/1‑68 (MQ=255) aTGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGAt < 1:11886753/68‑1 (MQ=255) tGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGAtt > 1:37186281/1‑67 (MQ=255) gACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTATGCCGCCTGATag > 1:4880444/1‑68 (MQ=255) gACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCCCCTGATag > 1:11049827/1‑68 (MQ=255) gACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATag < 1:50778363/68‑1 (MQ=255) gACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATag < 1:17234970/68‑1 (MQ=255) gACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATag < 1:31344496/68‑1 (MQ=255) aCCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCGACTGATtga > 1:42386537/1‑68 (MQ=255) ccATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTATGCCGCCTTATagaa > 1:43676217/1‑68 (MQ=255) ccATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATaga < 1:32987707/67‑1 (MQ=255) ccATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATaga < 1:20846823/67‑1 (MQ=255) aTAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATagaaga > 1:33843882/1‑68 (MQ=255) aCCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAgg < 1:12463782/68‑1 (MQ=255) aCCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAgg < 1:20214079/68‑1 (MQ=255) aCCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAgg < 1:24289048/68‑1 (MQ=255) ccGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCCGCTGATAGAAGAAGGt > 1:28952114/1‑68 (MQ=255) cGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTa > 1:15057256/1‑68 (MQ=255) cGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTa < 1:3411556/68‑1 (MQ=255) cGAATTTACCCATCCATTCGGAGAGGCGATCGATCACCATATCGTAGGCCACCTTATAGTAGAAGTTa > 1:37410091/1‑68 (MQ=255) aTTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATcc < 1:38045169/68‑1 (MQ=255) aTTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATcc < 1:28279000/68‑1 (MQ=255) tACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCCGCTGATAGATGAAGTTATCAGcg > 1:24534247/1‑67 (MQ=255) tACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCa < 1:50206471/68‑1 (MQ=255) gatccatTCGTAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGAtt < 1:19728757/67‑1 (MQ=255) catccatTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTGTCCGCAGAtt < 1:49443918/68‑1 (MQ=255) catccatTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGAtt < 1:1915562/68‑1 (MQ=255) catccatTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGAtt < 1:32143748/68‑1 (MQ=255) tccatTCGGAGAGGCGATCGATCCCCATATCGTATGCCGGCGGAGACAAGAAAGAAACAGCAGAt > 1:19882204/1‑65 (MQ=255) tccatTCGGAGAGGCGATCGATCCCCATATCGTAGGCCCGCTGATAGAAGAAGGTATACGCAGAttct > 1:30300964/1‑68 (MQ=255) tccatTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGAttct < 1:44856022/68‑1 (MQ=255) ccatTCGGAGAGGCGATCGATCCCCATATCGTATGCCACCTGATAGAAGAAGGTATACTCAGAttct > 1:35383634/1‑67 (MQ=255) ccatTCGGAGAGGCGATCGATCCCCATATCGTAGGCCAGCTGATTGAAGAAGTTATCAGCAGAttct > 1:2969085/1‑67 (MQ=255) catTCGGAGAGGCGATCGATCCCCATATCGTATGCCGGCTGATTGATGAATTTATAAGCAGAttctt > 1:50351993/1‑67 (MQ=255) cGGAGAGGCGATCGATCCCCATATCGTATGCCACCTGATAGATGAAGGTATCCGCAGATTCTTCCAGc < 1:21865101/68‑1 (MQ=255) cGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGc < 1:37757164/68‑1 (MQ=255) ggAGAGGCGATCGATCCCCATATCGTAGGCCGACTTCTAGATGAAGGTATAAGCAGGTTGTTCCGGCg > 1:25175428/1‑68 (MQ=255) ggAGAGGCGATCGATCCCCATATCGTAGGCCCCCTGATTGAAGAAAGAATACGCAGGTTCTTGCAGCg > 1:42224371/1‑68 (MQ=255) gagaGGCGATCGATCCCCATATCGTAGGCCGACTGATACAAGAAAATAACAGCAGATTCTTCCAGCgc > 1:17667486/1‑67 (MQ=255) agagGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGa < 1:11336053/67‑1 (MQ=255) gatgCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGAt < 1:36332012/64‑1 (MQ=255) gagGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGGAGATTCTTCCAGCGAt < 1:23258318/67‑1 (MQ=255) gagGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGAt < 1:21265593/67‑1 (MQ=255) gagGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGAt < 1:47561028/67‑1 (MQ=255) gagGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGAt < 1:51159189/67‑1 (MQ=255) gagGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGAt < 1:4418518/67‑1 (MQ=255) gagGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGAt < 1:9035420/67‑1 (MQ=255) ggCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATc < 1:4511516/66‑1 (MQ=255) ggCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATc < 1:5569301/66‑1 (MQ=255) ggCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATc < 1:4041135/66‑1 (MQ=255) ggCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCt < 1:26374794/67‑1 (MQ=255) gatcgatcCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTtgt < 1:622624/67‑1 (MQ=255) gatcgatcCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTtgt < 1:42005634/67‑1 (MQ=255) gatcgatcCCCAAATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTtgtg > 1:46556179/1‑68 (MQ=255) cgatcCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACa < 1:41444852/68‑1 (MQ=255) gatcCCCATATCGTATGCCCGCTGATTGAAGAATTTATAAGCAGGTTCTTGCGGCGGTCTTGTGGCag > 1:16237353/1‑67 (MQ=255) gatcCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACAt > 1:15764536/1‑68 (MQ=255) gatcCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACAt < 1:22752516/68‑1 (MQ=255) tcCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTc < 1:4708834/68‑1 (MQ=255) cccATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAg < 1:40914505/68‑1 (MQ=255) tatCGTATGCCCACTTATAGAAGAAATTATAAGCAGATTCTTCCGGCGATCTTGTGGCATTCAGCCg > 1:38328258/1‑67 (MQ=255) tatCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGATGc < 1:35136032/68‑1 (MQ=255) ttCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGcc < 1:6902102/67‑1 (MQ=255) atCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGcc < 1:18199206/68‑1 (MQ=255) gTAGGCCACCTGATAGAAGAAGGTATCAGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGt > 1:17976530/1‑68 (MQ=255) | TACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGC > CP009273/662419‑662553 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |