BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	692,043	T→A	5.2%	noncoding (30/75 nt)	glnV ←	tRNA‑Gln

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	692,043	0	T	A	5.2%	1606.7 / 20.1	524	noncoding (30/75 nt)	glnV	tRNA‑Gln
Reads supporting (aligned to +/- strand): ref base T (316/173); new base A (18/16); total (335/189)
Fisher's exact test for biased strand distribution p-value = 1.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 546 total aligned reads displayed.

AAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACA  >  CP009273/691976‑692110

aaGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAAt                                                                     <  1:17616686/68‑1 (MQ=255)

  ggTAAGCGTCTTGGATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:49958355/1‑68 (MQ=255)

  ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:26972891/1‑68 (MQ=255)

  ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:40778464/1‑68 (MQ=255)

  ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:4246214/1‑68 (MQ=255)

  ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:39506706/1‑68 (MQ=255)

  ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:38797224/1‑68 (MQ=255)

  ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:45135680/1‑68 (MQ=255)

  ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:3707152/1‑68 (MQ=255)

  ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:46544986/1‑68 (MQ=255)

  ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:35953411/1‑68 (MQ=255)

  ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:33308307/1‑68 (MQ=255)

  ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:1476332/1‑68 (MQ=255)

  ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:50110971/1‑68 (MQ=255)

  ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:6251678/1‑68 (MQ=255)

  ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:35808953/1‑68 (MQ=255)

  ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:35339381/1‑68 (MQ=255)

  ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:9112293/1‑68 (MQ=255)

  ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACATCGGAATGCCGGAATCAGAATcc                                                                   >  1:2830119/1‑68 (MQ=255)

  ggTAAGCGTCTTGAATAAATTGGCTCTTGGACGAGGAGTCGAACCTCGGATTGACTGAATGAGAATcc                                                                   >  1:20518044/1‑68 (MQ=16)

   gTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCg                                                                  >  1:31584328/1‑68 (MQ=255)

   gTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAAACCg                                                                  >  1:26652813/1‑68 (MQ=255)

    tAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCgg                                                                 >  1:44385597/1‑68 (MQ=255)

    tAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCgg                                                                 >  1:30190719/1‑68 (MQ=255)

    tAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCACGGAATGCCGGAATCAGAATACgg                                                                 >  1:214746/1‑68 (MQ=255)

    tAAGCGTCTTGAATAAATTGGCTGGGGTACCAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCgg                                                                 >  1:7168480/1‑68 (MQ=255)

     aaGGGTCTTTAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGt                                                                <  1:22914993/68‑1 (MQ=255)

      aGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGt                                                                >  1:15319549/1‑67 (MQ=255)

        cGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCATAATCCGGTg                                                               >  1:34361684/1‑66 (MQ=255)

        cGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTg                                                               >  1:23697028/1‑66 (MQ=255)

        cGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTg                                                               >  1:32421516/1‑66 (MQ=255)

        cGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTg                                                               >  1:12142900/1‑66 (MQ=255)

        cGTCTTGAATAAATTGGCTGGGGTACGAGGATTCCAACCTCGGAAAGCCGGAATCAGAATCCGCTg                                                               >  1:45732429/1‑66 (MQ=17)

         ttCTTTAATAAATTGGCTGGGGTACGAGGATTCGAACCTGGGAATGCCGGTATCAAAAACCGGTGcc                                                             <  1:48702812/66‑1 (MQ=14)

         gTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCt                                                            >  1:18328217/1‑68 (MQ=255)

         gTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCt                                                            >  1:24898878/1‑68 (MQ=255)

         gTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAAACCGGTGCCt                                                            >  1:40125026/1‑68 (MQ=255)

          tCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCtt                                                           >  1:1007817/1‑68 (MQ=255)

             ttaaTAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTAcc                                                        <  1:6741008/66‑1 (MQ=255)

             ttaaTAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTAcc                                                        <  1:10529613/66‑1 (MQ=255)

               aaTAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGc                                                      <  1:24434714/68‑1 (MQ=255)

                aTAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCt                                                     >  1:966839/1‑68 (MQ=40)

                aTAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCt                                                     <  1:29029699/68‑1 (MQ=40)

                aTAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCt                                                     >  1:17210573/1‑68 (MQ=40)

                  aaaTTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTg                                                   >  1:22175641/1‑68 (MQ=37)

                   atttGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTgg                                                  <  1:32847812/66‑1 (MQ=14)

                    aTTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                                 <  1:50706362/68‑1 (MQ=33)

                    aTTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                                 <  1:11812043/68‑1 (MQ=33)

                     ttGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCg                                                >  1:16444614/1‑68 (MQ=31)

                     ttGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCg                                                >  1:19456910/1‑68 (MQ=31)

                     ttGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCg                                                >  1:17748261/1‑68 (MQ=31)

                     ttGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCATGTCg                                                >  1:33619640/1‑68 (MQ=255)

                                aCGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCcaaca                                     <  1:7852466/68‑1 (MQ=31)

                                   aGGATTCGAAGCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAt                                  >  1:50611322/1‑68 (MQ=255)

                                   aGGATTCGAACCTCGGAATGCGGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACaaaa                                  >  1:5818165/1‑67 (MQ=255)

                                   aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCcaacattt                                  >  1:33920344/1‑65 (MQ=14)

                                   aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCcaacattt                                  >  1:47904107/1‑65 (MQ=14)

                                   aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCcaacagtg                                  >  1:49143796/1‑65 (MQ=14)

                                   aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAt                                  >  1:2530809/1‑68 (MQ=37)

                                   aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAt                                  >  1:36462984/1‑68 (MQ=37)

                                   aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAt                                  >  1:42260917/1‑68 (MQ=37)

                                     gATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACATAtt                                 >  1:4469444/1‑67 (MQ=18)

                                       ttCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGt                              <  1:43638755/68‑1 (MQ=255)

                                       ttCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGt                              <  1:29792438/68‑1 (MQ=255)

                                       ttCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGt                              <  1:38165117/68‑1 (MQ=255)

                                       ttCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGt                              <  1:35100734/68‑1 (MQ=255)

                                            aCCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTAATTGGtatttc                         >  1:32043687/1‑66 (MQ=11)

                                            aCCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGa                         >  1:47133439/1‑68 (MQ=255)

                                                cGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAAttt                     <  1:13585662/68‑1 (MQ=255)

                                                   aaTGCCGGAATCCCAATCCGGAGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGc                   <  1:18811705/67‑1 (MQ=18)

                                                   aaTGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGc                   <  1:22561455/67‑1 (MQ=255)

                                                   aaTGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGc                   <  1:19997410/67‑1 (MQ=255)

                                                    aTGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAATTTGGTTTTGAATTTGcc                  >  1:5142386/1‑67 (MQ=255)

                                                    aTGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGATTTGAATTTGCCg                 >  1:2118644/1‑68 (MQ=255)

                                                     tGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGa                <  1:4321741/68‑1 (MQ=255)

                                                      gCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGaa               <  1:14463705/68‑1 (MQ=255)

                                                      gCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGaa               <  1:43128332/68‑1 (MQ=255)

                                                      gCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGaa               <  1:19621601/68‑1 (MQ=255)

                                                      gCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGaa               <  1:12924270/68‑1 (MQ=255)

                                                      gCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGa                >  1:2328170/1‑67 (MQ=255)

                                                      gCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAATGGATGTGAATTTGccta                >  1:28620505/1‑65 (MQ=17)

                                                       ccGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTGTTGAATTTTACGAAc              >  1:30961362/1‑68 (MQ=18)

                                                        cGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAAc              <  1:29282332/67‑1 (MQ=255)

                                                        cGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACa             <  1:12661808/68‑1 (MQ=255)

                                                         ggAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACat            <  1:41468458/68‑1 (MQ=255)

                                                         ggAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACat            <  1:13341520/68‑1 (MQ=255)

                                                         ggAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACat            <  1:3480985/68‑1 (MQ=255)

                                                           aaTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACata           <  1:11425361/67‑1 (MQ=255)

                                                           aaTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACata           <  1:4107477/67‑1 (MQ=255)

                                                           aaTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACata           <  1:26386666/67‑1 (MQ=255)

                                                            aTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACatat          <  1:48300694/67‑1 (MQ=255)

                                                            aTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACatat          <  1:8412465/67‑1 (MQ=255)

                                                            aTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACatat          <  1:3405383/67‑1 (MQ=255)

                                                            aTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACatat          <  1:44111034/67‑1 (MQ=255)

                                                            aTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACatat          <  1:46256392/67‑1 (MQ=255)

                                                             tCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTc        <  1:25665200/68‑1 (MQ=255)

                                                              cAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGATCATATTCg       >  1:37805695/1‑68 (MQ=255)

                                                                  aTCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGa      <  1:39898489/65‑1 (MQ=255)

                                                                  aTCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATa    <  1:15890489/67‑1 (MQ=255)

                                                                   tCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACa  <  1:27379761/68‑1 (MQ=255)

AAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACA  >  CP009273/691976‑692110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`