| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 999,598 | A→C | 6.0% | T66P (ACG→CCG) | ycbF → | putative periplasmic pilin chaperone |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 999,598 | 0 | A | C | 6.0% | 1380.1 / 9.3 | 506 | T66P (ACG→CCG) | ycbF | putative periplasmic pilin chaperone |
| Reads supporting (aligned to +/- strand): ref base A (336/132); new base C (3/35); total (339/167) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.29e-15 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 508 total aligned reads displayed.
TATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTAT > CP009273/999533‑999662 | atCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTACCAGTAAAATCAACCGCCCAACGC < 1:15889642‑M1/68‑1 (MQ=255) atCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGC < 1:25522612‑M1/68‑1 (MQ=255) atCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGC < 1:6421481‑M1/68‑1 (MQ=255) tCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCG < 1:11778925‑M1/68‑1 (MQ=255) cTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGT < 1:25293179‑M1/68‑1 (MQ=255) cTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGT > 1:14996503‑M1/1‑68 (MQ=255) ttttACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAACTCAACCGCCCACCGCGTTGG < 1:3286303‑M1/68‑1 (MQ=255) ttttACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGG < 1:3744929‑M1/68‑1 (MQ=255) ttttACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGG < 1:1523568‑M1/68‑1 (MQ=255) tttACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGG < 1:7850467‑M1/68‑1 (MQ=255) aCTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCg > 1:28506373/1‑68 (MQ=255) aCTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCg > 1:14903511/1‑68 (MQ=255) aCTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCg > 1:17696090/1‑68 (MQ=255) aCTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCg > 1:27509303/1‑68 (MQ=255) aCTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCg > 1:2221166/1‑68 (MQ=255) ctcaCTAATCCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTGGGGCCgg < 1:5191368/65‑1 (MQ=255) tGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCACCCCCCCACCGCGTTGGGCCggg < 1:28907725/68‑1 (MQ=255) tGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCggg > 1:29725817/1‑68 (MQ=255) cTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCgggggg > 1:1379358/1‑68 (MQ=255) cTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCgggggg > 1:6802828/1‑68 (MQ=255) cTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCgggggg > 1:1921128/1‑68 (MQ=255) aaTACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGa > 1:20595715/1‑68 (MQ=255) tACCAGTCAGGAATCCTGGCTTATAAACAGAAAAATCAACCGCCCACCGCGGTGGGCCGGGGGGGAAg < 1:25648194/68‑1 (MQ=255) aCCAGTCAGGAATCCTGGCTTGTTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:20020746/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTGACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:3588483/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:26072790/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:937322/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:22531519/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:2340176/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:2431789/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:24914990/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:8749984/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:8258296/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:25842141/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:26312919/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:5877215/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:27925713/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:5681701/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:28749726/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:4963563/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:29278730/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:10321223/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:4741164/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:4609796/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:21682684/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:10044616/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:10797167/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:13561786/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:15475762/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:16124154/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:16607491/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:18875505/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:18971109/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGACCAACGCGTTGGGCCGGGGCGGAAGc > 1:5486471/1‑68 (MQ=255) aCCAGTCAGCAATCCTGGCTTATTAACAGTAAAAACAACCGCCCAACGCGTTGGGCCGGGGGGGAAga > 1:28375137/1‑67 (MQ=255) ccAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCg > 1:3050408/1‑68 (MQ=255) cAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCg > 1:19324749/1‑67 (MQ=255) cAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCg > 1:23753785/1‑67 (MQ=255) tCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCg > 1:20216146/1‑67 (MQ=255) tCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCg > 1:19645010/1‑67 (MQ=255) tCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCg > 1:9137630/1‑67 (MQ=255) tCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCg > 1:10549045/1‑67 (MQ=255) tCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCg > 1:11023303/1‑67 (MQ=255) cAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCggttgattttactgtta < 1:12249585‑M1/67‑18 (MQ=255) cAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGa < 1:24789522/67‑1 (MQ=255) cAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTGGGGCggttgattttactgt < 1:22915460‑M1/65‑16 (MQ=255) aaTCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGGTGGGCCGGGGGGGAAGCGTCGACAGTg < 1:21968876/68‑1 (MQ=255) aaTCCTGGCTTATAAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGt < 1:14229410/67‑1 (MQ=255) aTCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTg > 1:11471830/1‑67 (MQ=255) aTCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTg < 1:14051699/67‑1 (MQ=255) aTCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTg > 1:442615/1‑67 (MQ=255) aTCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTg > 1:2733725/1‑67 (MQ=255) aTCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTg > 1:17405210/1‑67 (MQ=255) aTCCTGGATTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTg < 1:21057329/67‑1 (MQ=255) ggCTTATTAACAGTAAAATCACCCGCCCCACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCa < 1:18215517/68‑1 (MQ=255) ggCTTATTAACAGTAAAATCAACCGCCCACCGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCa < 1:11291869/68‑1 (MQ=255) ggCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCa < 1:8036891/68‑1 (MQ=255) gCTTATAAACATTAAAATCAACCCCCCACCGCGTGGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCa < 1:27702042/67‑1 (MQ=255) ccTTATTAACAGTAAAAACAACCCCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCa < 1:896284/66‑1 (MQ=255) cTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCAcc > 1:14508336/1‑68 (MQ=255) cTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCAcc > 1:17301546/1‑68 (MQ=255) cTTATTAACAGTAAAATCAAACGCCAAACGCGTTGGGCCGGGGGGGAAGCGTCGAAAGTTCCAGAAca > 1:24005201/1‑67 (MQ=255) taACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCAtt > 1:4096608/1‑66 (MQ=255) aCAGTAAAATCAACCGCCCACCGGGTTGGGCggttgattttacggttaataagccaggattcctg > 1:21335249‑M1/1‑31 (MQ=255) ataaaCCGCCCAACGCGTTGGGCCGGGGGGGAAGCCTCGACAGTGCCAGCACCATTACTGGCCGCAcc > 1:7183082/4‑68 (MQ=255) aTCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTcc > 1:16420100/1‑68 (MQ=255) aTCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTcc < 1:13057050/68‑1 (MQ=255) cacccGCCCACCGCGTGGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGc < 1:24464482/65‑1 (MQ=255) cAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCg > 1:12404372/1‑67 (MQ=255) cAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCg > 1:7450153/1‑67 (MQ=255) aCCGCCCAACGCGTTGGGCggttgattttactgttaataagccaggattcctgactggtattaggcag > 1:26731988‑M1/1‑19 (MQ=255) aCCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGcc > 1:18517151/1‑67 (MQ=255) aCCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGcc > 1:20796712/1‑67 (MQ=255) cGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACt > 1:7343516/1‑68 (MQ=255) gCCCACCGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACtt < 1:23259326/68‑1 (MQ=255) gCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACtt > 1:12673780/1‑68 (MQ=255) cccAACGCGTTGGGCggttgattttactgttaataagccaggattcctgactggtattagtcagtaaa > 1:18029134‑M1/1‑15 (MQ=255) cccAACGCGTTGGGCggttgattttactgttaataagccaggattcctgactggtattagtcagtaaa > 1:16836773‑M1/1‑15 (MQ=255) ccAACGCGTTGGGCggttgattttactgttaataagccaggattcctgactggtattagtcagtaaaa > 1:6100813‑M1/1‑14 (MQ=255) ccAACGCGTTGGGCggttgattttactgttaataagccaggattcctgactggtattagtcagtaaaa > 1:26980563‑M1/1‑14 (MQ=255) | TATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTAT > CP009273/999533‑999662 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |