Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,511,621 C→G 5.6% intergenic (‑26/+14) alaX ← / ← alaW tRNA‑Ala/tRNA‑Ala

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,511,6210CG 5.6% 1294.4 / 6.7 433intergenic (‑26/+14)alaX/alaWtRNA‑Ala/tRNA‑Ala
Reads supporting (aligned to +/- strand):  ref base C (263/140);  new base G (20/9);  total (284/149)
Fisher's exact test for biased strand distribution p-value = 8.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 436 total aligned reads displayed.

TGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGC  >  CP009273/2511555‑2511687
                                                                  |                                                                  
  catgccatgcAAGCGCTCTCCCAGCTGAGCTATAGCCCCCCGAGGCGTTTACGTTCCAAGTTTGCggg                                                                 >  1:7548240/1‑68 (MQ=18)
  catgccatgcAAGCGCTCTCCCAGCTGAGCTATAGCCCCCCGAGGCGTTTACGTACGAAGTTTGCTgg                                                                 >  1:26656574/1‑68 (MQ=21)
     gccatgcAAGCGCTCTCCCAGCTGAGCTATAGCCCCGGGATGGGTTTAGGTACCAAGTTTGCTGGGt                                                               >  1:29170179/1‑67 (MQ=18)
     gccatgcAAGCGCTCTCCCAGCTGAGCTATAGCCCCGCGATGGGTTTACGTTCGAAGTTTGGTGGGt                                                               >  1:28761471/1‑67 (MQ=18)
     gccatgcAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGt                                                               >  1:3463932/1‑67 (MQ=255)
     gccatgcAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGt                                                               >  1:24854455/1‑67 (MQ=255)
      ccatgcAAGCGCTCTCCCAGCTGAGCTATAGCCCCGCGATGGGTTTAAGTACCAAGTTTGGTGGGGGc                                                             >  1:12953862/1‑68 (MQ=18)
           cAAGCGCTCTCCCAGCTGAGCTATAGCCCCTCGAGGCAATTAGGTACCAATTTTCCTGGG‑GGAAAAtt                                                       >  1:17858738/1‑68 (MQ=9)
           cAAGCGCTCTCCCAGCTGAGCTATAGCCCCAGGATGCGTTTACGTACCAAGTTTGCTGGGTGCaaatt                                                        >  1:13455810/1‑66 (MQ=255)
             aGCGCTCTCCCAGCTGAGCTATAGCCCCGCCATGCGTTTACTGACCAAGTTACCTGCGTGCAAAAttt                                                      >  1:20188508/1‑68 (MQ=18)
             aGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAAttt                                                      >  1:20514881/1‑68 (MQ=255)
               cgcTCTCCCAGCTGAGCTATAGCCCCGGGAGGCGTTTACGTACCAAGTTTGCTGGGTGATAAATTtgg                                                    >  1:17759191/1‑68 (MQ=25)
               cgcTCTCCCAGCTGAGCTATAGCCCCCCGAAGCGTTTACGTACCAAGTTTCCTGGGTCAAAAGtttcg                                                    >  1:4401493/1‑66 (MQ=21)
               cgcTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTtgg                                                    >  1:12433908/1‑68 (MQ=255)
               cgcTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCGGGGTGCAAAATTtgg                                                    >  1:16352267/1‑68 (MQ=255)
               cgcTCTCCCAGCTGAGCTATAGCCCCAAGATGCGTTTACGTACCAAGTTTGCTGCGTGCAAAATTtgg                                                    >  1:7929809/1‑68 (MQ=38)
                gcTCTCCCAGCTGAGCTATAGCCCCCGCATGCGTTTACGTACCAAGTTTCCTGGGGGCAAAAatttat                                                   >  1:20669528/1‑65 (MQ=21)
                gcTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTTCCAAGTTTGCTGGGTGCTAAATTtggt                                                   >  1:21006468/1‑68 (MQ=255)
                gcTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTtggt                                                   >  1:524216/1‑68 (MQ=255)
                gcTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTtg                                                     <  1:1557881/66‑1 (MQ=255)
                gcTCTCCCAGCTGAGCTATAGCCCCACGAGGCGTTTACGTACCAAGTTTCGTGGGTGCAAAATTtggt                                                   >  1:10390099/1‑68 (MQ=37)
                   ctcCCAGCTGAGCTATAGCCCCACGATTCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGa                                                <  1:17947816/68‑1 (MQ=255)
                   ctcCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGa                                                <  1:26928927/68‑1 (MQ=255)
                    tcCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGa                                                >  1:1661181/1‑67 (MQ=255)
                     cccAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGc                                              <  1:13266264/68‑1 (MQ=255)
                        atatGAGCTAAAGCCACACTAATCGTTTACGTACCAAGCATGCTGGGTTCTAAATTTGGTGGAGCTaa                                           <  1:26123075/65‑1 (MQ=255)
                        agatGAGCTATAGCCCCACGATTCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTaa                                           <  1:18737485/65‑1 (MQ=255)
                        aGCTGAGCTATAGCCCCGCGAGGAGTTTACGTTCCAAGTTACCTGGGTGAAAAATTTGGTTGAGCtca                                           >  1:5934289/1‑66 (MQ=255)
                        aGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTaa                                           >  1:5135362/1‑68 (MQ=255)
                        aGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTCCTGGGTGCAAAATTTGGTGGAGCTaa                                           >  1:24385739/1‑68 (MQ=255)
                        aGCTGAGCTATAGCCCCACGAGGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTTGAGCTaa                                           >  1:11691681/1‑68 (MQ=255)
                         gCTGAGCTATAGCCCCCCGATGGGTTTACGTACCAAGTTTGCTGGGTGCTAAATTTAGTGGAGCTaa                                           >  1:1488710/1‑67 (MQ=255)
                          cTGAGCTATAGCCCCCCGATGCGTTTACGTACCAAGTTTGCGGGGTGCAAAATTTGGTGGAGCTAAGc                                         >  1:25068147/1‑68 (MQ=39)
                          cTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGc                                         >  1:14966199/1‑68 (MQ=255)
                          cTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGc                                         >  1:11213867/1‑68 (MQ=255)
                           tGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTTGAGCTAAGCg                                        >  1:8507724/1‑68 (MQ=255)
                           tGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTaa                                           <  1:16825618/65‑1 (MQ=255)
                            gAGCTATAGCCCCCCGATGCGTTTAAGTACCAAGTTTGCTGGGTGCAAAATTTGGTTGAGCTAAGCgg                                       >  1:3920198/1‑68 (MQ=255)
                              gcaataGCCCCACGATGCGTTTACGCACCAAGGATGCTGGGTGCAAAATTTGGTGGAGCTAAGCggg                                      <  1:19916057/64‑1 (MQ=37)
                              gCTATAGCCCCACGATTCGTTTACGTACCAAGTTTGCTGGGTTCAAAATTTGGTGGAGCTAAGCggg                                      <  1:23519692/67‑1 (MQ=38)
                                tataGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATc                                   >  1:3589282/1‑68 (MQ=255)
                                tataGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATc                                   >  1:19137405/1‑68 (MQ=255)
                                 ataGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCg                                  >  1:4895587/1‑68 (MQ=255)
                                   aGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGa                                 >  1:15993983/1‑67 (MQ=255)
                                   aGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGa                                 >  1:26367043/1‑67 (MQ=255)
                                   aGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGa                                 >  1:8256407/1‑67 (MQ=255)
                                   aGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGa                                 >  1:14427804/1‑67 (MQ=255)
                                   aGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGa                                 >  1:15214061/1‑67 (MQ=255)
                                   aGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGa                                 >  1:18611402/1‑67 (MQ=255)
                                    gCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGaa                                >  1:29507862/1‑67 (MQ=255)
                                    gCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGaa                                >  1:27050037/1‑67 (MQ=255)
                                     ccccACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAAcc                              >  1:14616568/1‑68 (MQ=255)
                                     ccccACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAAcc                              >  1:11938523/1‑68 (MQ=255)
                                     ccccACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAAcc                              >  1:18823131/1‑68 (MQ=255)
                                     ccccACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAAcc                              >  1:273669/1‑68 (MQ=255)
                                     ccccACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAAcc                              >  1:12170918/1‑68 (MQ=255)
                                     ccccACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAAcc                              >  1:28931369/1‑68 (MQ=255)
                                     ccccACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAAcc                              >  1:6187747/1‑68 (MQ=255)
                                     ccccACGATGACATTACGTACCATGCATGGTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAAcc                              <  1:25407448/68‑1 (MQ=18)
                                      cccACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAAcc                              >  1:1711525/1‑67 (MQ=255)
                                      cccACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAAcc                              >  1:7191808/1‑67 (MQ=255)
                                       ccACGATGCGTTTATGTTCCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGc                            <  1:21735631/68‑1 (MQ=255)
                                       ccACGATGCGTTAACGTACCAAGCTTGCTGGGTGCTAAATTTGGTGGAGCTAAGCGGGATCGAACCGc                            <  1:25750934/68‑1 (MQ=25)
                                        cACGATGCGTTTACGTACAAAGTTTGCTGCGTGCAAAATTTGGTGGATCTAAGCGGGATCGaaac                              >  1:21246916/1‑63 (MQ=37)
                                          cgctGCGTTTACGCACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGa                         <  1:17644838/65‑1 (MQ=255)
                                          cGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGa                         <  1:2214036/68‑1 (MQ=255)
                                          cGATGCGATTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGa                         <  1:17403723/68‑1 (MQ=255)
                                           gATTCGTTTACGTACCAAGTTTCCTGGGTTGAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGAc                        <  1:9489960/68‑1 (MQ=18)
                                           gATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGa                         >  1:25916812/1‑67 (MQ=255)
                                           gATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGa                         >  1:29693935/1‑67 (MQ=255)
                                           gATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGa                         >  1:28167672/1‑67 (MQ=255)
                                           gATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGa                         >  1:24054407/1‑67 (MQ=255)
                                             tGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGAcc                       >  1:8363136/1‑67 (MQ=255)
                                                gTTTACGTCCCAAGTATGCTGGGAGCTAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACctct                    <  1:14118096/67‑1 (MQ=18)
                                                gTTTACGTACCAAGTTTGCTGGGTTCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACctct                    <  1:29056840/67‑1 (MQ=255)
                                                gTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACctct                    <  1:3377672/67‑1 (MQ=255)
                                                gTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACctct                    <  1:8728006/67‑1 (MQ=255)
                                                   tACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGca                >  1:6502235/1‑68 (MQ=255)
                                                     cGTGCCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGcat               >  1:10513571/1‑67 (MQ=255)
                                                     cGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGcat               >  1:6866812/1‑67 (MQ=255)
                                                     cGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGcat               >  1:9555929/1‑67 (MQ=255)
                                                     cGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGcat               >  1:4053720/1‑67 (MQ=255)
                                                     cGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGcat               >  1:23820046/1‑67 (MQ=255)
                                                     cGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGcat               >  1:15873936/1‑67 (MQ=255)
                                                     cGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGcat               >  1:22998914/1‑67 (MQ=255)
                                                     cGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGcat               >  1:22200431/1‑67 (MQ=255)
                                                       tACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGcatgcc            >  1:4789494/1‑68 (MQ=255)
                                                        aCCAAGTTTGGTGGGTTGAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGcatgcca           <  1:19376055/68‑1 (MQ=14)
                                                        aCCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGcatgcca           >  1:10110024/1‑68 (MQ=255)
                                                        aCCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGcatgcca           >  1:16190859/1‑68 (MQ=255)
                                                        aCCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGcatgcca           >  1:18150774/1‑68 (MQ=255)
                                                        aCCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGcatgcc            <  1:18411881/67‑1 (MQ=255)
                                                        aCCAAGTATGCTGGGTGCTAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGcatgcc            <  1:12631324/67‑1 (MQ=255)
                                                          cAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGcatgccatg         <  1:18298794/68‑1 (MQ=255)
                                                            aGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCa       >  1:2270852/1‑68 (MQ=255)
                                                            aGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCa       >  1:13745297/1‑68 (MQ=255)
                                                              tttGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCAtgcaatgcaa      >  1:5528990/1‑67 (MQ=255)
                                                                tGCTGGGTGGAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAgcg   <  1:10810457/68‑1 (MQ=255)
                                                                tGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAgcg   >  1:7335610/1‑68 (MQ=255)
                                                                tGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAgc    >  1:27863755/1‑67 (MQ=255)
                                                                  |                                                                  
TGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGC  >  CP009273/2511555‑2511687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑