| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 2,711,673 | A→C | 10.7% | V72G (GTT→GGT) | yfiF ← | putative methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,711,673 | 0 | A | C | 10.7% | 642.3 / 17.9 | 252 | V72G (GTT→GGT) | yfiF | putative methyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (155/67); new base C (4/26); total (159/93) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.13e-09 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 258 total aligned reads displayed.
AAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGG > CP009273/2711607‑2711740 | aaaCTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAAc < 1:10136326/68‑1 (MQ=255)aaaCTTTTACCACTGATGCCACCGTGAGCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAAc < 1:3284830/68‑1 (MQ=255) aCTTTTACCACTGATGCCCCCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAACCCg > 1:9017748/1‑68 (MQ=255) aCTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCg > 1:15216478/1‑68 (MQ=255) aCTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCg > 1:28616006/1‑68 (MQ=255) aCTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCg > 1:28279792/1‑68 (MQ=255) aCTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCg > 1:17751498/1‑68 (MQ=255) aCTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCg > 1:18445236/1‑68 (MQ=255) aCTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCg > 1:1914667/1‑68 (MQ=255) aCTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGCGAAACCg > 1:15566159/1‑68 (MQ=255) cTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGt < 1:19367706/68‑1 (MQ=255) cTTTTACCACTGATGCCACCGTGAGCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCTCGGGAAACCGt < 1:15539933/68‑1 (MQ=255) ttttACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCAGC‑GCGCGGGAACCCGt > 1:3133673/1‑67 (MQ=255) ttACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTgcg < 1:12979122/68‑1 (MQ=255) aCCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGcc > 1:955029/1‑68 (MQ=255) ccACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTgcgca > 1:7113113/1‑66 (MQ=255) ccACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGcc > 1:25547384/1‑67 (MQ=255) ccACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGcc > 1:22166703/1‑67 (MQ=255) ccACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGcc > 1:165374/1‑67 (MQ=255) ccACAGATCCCACCGTGATCGGCCATTTCCCCCGTCTCATCACCCGGC‑GCGCGGGAAACCCTGCGcc > 1:20487270/1‑67 (MQ=255) cACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCAc > 1:23101351/1‑68 (MQ=255) cACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCAc > 1:23595804/1‑68 (MQ=255) cACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCAc > 1:25162652/1‑68 (MQ=255) cACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCAc > 1:27805443/1‑68 (MQ=255) cACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCAc > 1:15045716/1‑68 (MQ=255) cACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGA‑GCGCGGGAAACCGTGCGCCAc > 1:5294595/1‑68 (MQ=255) aCTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGccggg < 1:19579837/68‑4 (MQ=255) aCTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCACg > 1:28389857/1‑68 (MQ=255) aCTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCACg > 1:26420129/1‑68 (MQ=255) tGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCACGGCGaa > 1:6991810/1‑68 (MQ=255) tGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCACGGCGaa > 1:27600437/1‑68 (MQ=255) tGCCACCGTGATCGCCCTTTGCCGGCTTATCATAACCCGGC‑GCGGGTAAAACCGGGCGCCACGGCGaa < 1:64009/68‑1 (MQ=255) cccccGTGAGCGGCCTTTCCGGGCGTCCCACCACCCGGC‑GCGCGGGAAACCGGGCGCCACGGCGAATc < 1:130139/65‑1 (MQ=255) ccACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCACGGCGaa > 1:21219859/1‑66 (MQ=255) ccACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCACGGCGaa > 1:2594992/1‑66 (MQ=255) ccACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCACGGCGaa > 1:28081572/1‑66 (MQ=255) ccccGTGATCGGCCTTTTCCGGCGGCCCAACCCCCGGC‑GCGCGGGAACCCGGGCGCCACGGCGAATCt < 1:14208590/66‑1 (MQ=255) ccccGGGAGCGGCCTTTCCCGGCGCCCCATCACCCGGC‑GCGGGGGAAACCGTGCGCCACGGCGAATCt < 1:21876203/66‑1 (MQ=255) cACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCt > 1:16361559/1‑68 (MQ=255) aCCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCt > 1:18085502/1‑67 (MQ=255) ccGTGTTGGGCATTTTCGGGGGTCTCATCACCCGGC‑GCGCGGGAAACCGGGCGCCACGGCGAATCTTc < 1:5743495/68‑1 (MQ=255) ccGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCtt > 1:19923451/1‑67 (MQ=255) ccGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCtt > 1:24371193/1‑67 (MQ=255) ccGTGAGCGGCCTTTGCCGGCGTCTCAGCCCCCGGC‑GCGGGGTAACCCGTGCGCCACGGCGAATCTTc < 1:13202599/68‑1 (MQ=255) cGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTc > 1:2030834/1‑67 (MQ=255) cGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTc > 1:29359230/1‑67 (MQ=255) gTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTccc > 1:25709274/1‑68 (MQ=255) gATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAACCCGTGCGCCACGGCGAATCTTccc > 1:9753611/1‑66 (MQ=255) gATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCACGGCGCATCTTccc > 1:7788050/1‑66 (MQ=255) gATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTccc > 1:17238687/1‑66 (MQ=255) aTCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACt > 1:10350040/1‑68 (MQ=255) tCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACtt > 1:15777954/1‑68 (MQ=255) tCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACtt > 1:3654871/1‑68 (MQ=255) gCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCg > 1:26959933/1‑68 (MQ=255) gCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCg > 1:16677948/1‑68 (MQ=255) ccTTTTCCGGCGTCTCATCACCCGGC‑GCCCGGGAAACCGTGCGCCACGGCGGATCTTCCCACTAGccg > 1:11876464/1‑66 (MQ=255) cTTTGCCGGCGGCTAAAC‑CCCGGCGGCGCGGGAAACGGTGCGCCACGGCGAATCTTCCCACTTGCGGt < 1:2546947/68‑1 (MQ=255) ttttCCGGCGTCTCACCCCCCGGC‑GCGCGGCAAACCGGGCGCCACGGCGAATCTTCCCACTTGCGGTc < 1:16764991/68‑1 (MQ=38) ttttCCGGCGTCTAAGCACCCGGC‑GCGGGGGAAACCGGGCGCCACGGCGAATCTTCCCACTTGCGGTc < 1:26774154/68‑1 (MQ=255) tttCCGGCGTCTCACCCCCGGGC‑GCGGGTTAAACGGTGCGCCACGGCGAATCTTCCCACTTGCGGTCa < 1:8048494/68‑1 (MQ=255) ccGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACgg > 1:3993484/1‑68 (MQ=255) ccGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACgg > 1:3468729/1‑68 (MQ=255) ccGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACgg > 1:11399281/1‑68 (MQ=255) ccGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACgg > 1:18179165/1‑68 (MQ=255) ccGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACgg > 1:29274670/1‑68 (MQ=255) cGGGGTCACACCCCCCGGC‑GCGGGTTAACCCGGGCCCCACGGCGAATCTTCCCACTTGCGGTCACggg < 1:7502944/68‑1 (MQ=255) cGGCGTCTCATCCCCCGGC‑GCGCGGTAACCCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACggg < 1:4635949/68‑1 (MQ=255) cGGCGTCTAAACCCCCGGC‑GCGCGGCAAACCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACggg < 1:8177682/68‑1 (MQ=37) cGGCGCCTCACCCCCCGGC‑GCGGGGCAACCCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACggg < 1:23747751/68‑1 (MQ=255) ggCTTCTCATCACCCGGC‑GGGGGGTAAACCGTGCCCCACGGCGAATCTTCCCACTTGCGGTCACgggg < 1:16950717/68‑1 (MQ=255) ggCGTCTCATCACCCGGC‑GCGGGGTAAACCGTGCGCCACGGCGAATCTTCCCACTCGCGGTCACgggg < 1:28815660/68‑1 (MQ=255) ggCGTCTCATCACCCGGC‑GCGCGGTAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACgggg < 1:14547460/68‑1 (MQ=255) ggCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACggg > 1:14366566/1‑67 (MQ=255) ggCGTCTCAACCCCCGGC‑GCGCGGTAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACgggg < 1:20952115/68‑1 (MQ=255) gggTCTCACCCCCCGGC‑GCGGGGTAACGCGGGCCCCACGGCGAATCTTCCCACTTGCGGTCACgggg < 1:23277630/65‑1 (MQ=255) ctcATCACCCGGG‑GCGGGGCAAACCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGa < 1:1972667/67‑1 (MQ=38) cccccGGC‑GGGCGGAAACCCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGt < 1:10930566/66‑1 (MQ=255) cccccGGC‑GCGCGGTAACCGGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGt < 1:13418365/66‑1 (MQ=255) cccccGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGt < 1:11727807/66‑1 (MQ=255) cccccGGC‑GCGCGGCAACCGGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGt < 1:21641999/66‑1 (MQ=255) caCCCGCC‑GCGCGGTAACCCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGt < 1:12677871/68‑1 (MQ=255) aCCCGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGtt > 1:2714006/1‑68 (MQ=255) aCCCGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGtt > 1:2131485/1‑68 (MQ=255) aCCCGCC‑GCGCGGTAAACCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGtt < 1:26736136/68‑1 (MQ=255) cccGGC‑GGGGGGCAACCCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGttt < 1:20822866/68‑1 (MQ=255) cccGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGttt > 1:8660815/1‑68 (MQ=255) cccGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGttt > 1:984423/1‑68 (MQ=255) cccGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGttt < 1:13178581/68‑1 (MQ=255) cccGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGttt > 1:24659669/1‑68 (MQ=255) cccGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGttt > 1:240356/1‑68 (MQ=255) cccGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGttt > 1:23479065/1‑68 (MQ=255) cccGGC‑GCGCGGGAAACCGTGCGCAAAGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGttt > 1:22440647/1‑68 (MQ=255) ccGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTg > 1:6689933/1‑68 (MQ=255) ccGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTg > 1:12594151/1‑68 (MQ=255) ccGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTg > 1:16227611/1‑68 (MQ=255) gcgggGGAACCCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCAt < 1:4778032/67‑1 (MQ=255) aaCCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCgcgggcgg > 1:15730346/1‑68 (MQ=255) aaCCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCgcgggcgg > 1:18677156/1‑68 (MQ=255) aaCCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCgcgggcgg < 1:10648198/68‑1 (MQ=255) aCCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGcgggcggg > 1:28679923/1‑68 (MQ=255) | AAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGG > CP009273/2711607‑2711740 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |