| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,188,216 | C→T | 5.6% | noncoding (9/143 nt) | sibD ← | sRNA antisense regulator of toxic IbsD protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,188,216 | 0 | C | T | 5.6% | 1217.9 / 38.1 | 385 | noncoding (9/143 nt) | sibD | sRNA antisense regulator of toxic IbsD protein |
| Reads supporting (aligned to +/- strand): ref base C (184/167); new base T (4/17); total (200/185) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.10e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 438 total aligned reads displayed.
ATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATC > CP009273/3188149‑3188281 | aTTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATcctccctc > 1:17735544/1‑68 (MQ=255)aTTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATaatccctc > 1:1488970/1‑68 (MQ=255) tgtgTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCa > 1:26548865/1‑67 (MQ=255) tgtgTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCa > 1:1589884/1‑67 (MQ=255) tgtgTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCa > 1:15216176/1‑67 (MQ=255) gtgtTGTTACTCGTAAGTTTCGCTTCTTATTAACATCCAATCAGAGGGGGGAGAAATCCTCCCTTAcc < 1:25993098/68‑1 (MQ=18) gtgtTGTTACTCGTAAGTTTCGCTTCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTTAcc < 1:11587115/68‑1 (MQ=18) gtgtTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGTGGAGAAATCCTCCCTCAcc < 1:20170788/68‑1 (MQ=255) gtgtTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCAcc < 1:10445285/68‑1 (MQ=255) gtgtTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCAcc < 1:18866670/68‑1 (MQ=255) gtgtTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCAcc < 1:19043340/68‑1 (MQ=255) gtgtTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCAcc < 1:4057883/68‑1 (MQ=255) gtgtTGTTACTCGAAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCAcc < 1:8711292/68‑1 (MQ=255) tgttgtTACTCTTAAGTTTCGCATCTTATTAACAGCCAATCACATGGTGGAGACAGCCTCCCacac > 1:21096817/1‑66 (MQ=255) tgttgtTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTAAc > 1:13833452/1‑66 (MQ=255) ttgttACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTTAcc > 1:7760809/1‑65 (MQ=255) ttgttACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCtt > 1:27391201/1‑68 (MQ=255) ttgttACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTAACCCtt > 1:14006936/1‑68 (MQ=255) tgttACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCtt < 1:19875645/67‑1 (MQ=255) tgttACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTg > 1:11724763/1‑68 (MQ=255) tgttACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTg < 1:22844231/68‑1 (MQ=255) tACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGTGGAGAAATCCTCCCTCACCCTTGtt < 1:1414183/67‑1 (MQ=255) tACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGtt < 1:17371442/67‑1 (MQ=255) tACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTc < 1:5375510/68‑1 (MQ=255) tACTCGTAAGTTTCGCAGCTTATTAAAAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGtt < 1:23444804/67‑1 (MQ=255) aCTCTTAAGTTTCGCCCCTTACTAACACCCAATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTcc < 1:26251578/68‑1 (MQ=11) aCTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTcc < 1:6978532/68‑1 (MQ=255) aCTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTcc < 1:23309633/68‑1 (MQ=255) aCTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTcc < 1:4652983/68‑1 (MQ=255) cTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCct > 1:16899017/1‑68 (MQ=255) cTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCct < 1:19558748/68‑1 (MQ=255) cTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCct < 1:28373254/68‑1 (MQ=255) gTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCcttt > 1:18545819/1‑67 (MQ=255) aaGTTTCGCAGCTTATTAACATCCAATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCctttac < 1:3308039/67‑1 (MQ=18) aaGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCctttact > 1:11909993/1‑68 (MQ=255) aaGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCctttac < 1:8950149/67‑1 (MQ=255) aaGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCctttac < 1:10853975/67‑1 (MQ=255) aaGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCctttac < 1:24149494/67‑1 (MQ=255) aaGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCctttac < 1:6023702/67‑1 (MQ=255) aaGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCctttac < 1:25343425/67‑1 (MQ=255) aGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCctttactt > 1:1716980/1‑68 (MQ=255) gTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCctttacttt > 1:21939363/1‑68 (MQ=255) ttCGCAGCTTATTAACACCCAATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAg < 1:4464284/68‑1 (MQ=17) gCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTGTAGGtt > 1:24281727/1‑68 (MQ=255) aGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTGTAGGTTGa > 1:15000791/1‑68 (MQ=255) ttattaACAGCCAATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTcc < 1:24733183/50‑1 (MQ=255) ttattaACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGa < 1:29233049/65‑1 (MQ=255) attaACAGCCAATCAGAGGGGGGAGAAATCCTCCCTTAcc < 1:26909185/40‑1 (MQ=255) aaCAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGaaaaaaaa > 1:25532523/1‑67 (MQ=255) aaCAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGaaaaaaaa > 1:2961528/1‑67 (MQ=255) aaCAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTATAGGTTGaaaaaaca > 1:10186888/1‑65 (MQ=255) aaCAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTATAGGTTGaaaaaaca > 1:16606439/1‑65 (MQ=255) aaCAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCATTGTTCATTTACTATAGTTTGaaaaaaaa > 1:15385356/1‑67 (MQ=17) aaCAGCCAATCAGAGGGGGGAGAAATCCTCACTCACCCTTGTTCCTTTACTTTAGGTTGaaaaataa > 1:10959018/1‑67 (MQ=255) aCAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATc > 1:15640694/1‑68 (MQ=255) aGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCa > 1:21526604/1‑67 (MQ=255) aGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCa > 1:18312503/1‑67 (MQ=255) aGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTGTAGGTTGAAAAAAAAACa > 1:26093027/1‑67 (MQ=255) gCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTATAGGTTGAAAAAACAACAGc > 1:27680864/1‑68 (MQ=11) gCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTATAGGTTGAAAAAAAAACAGc > 1:20894775/1‑68 (MQ=255) cAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCg < 1:12228451/67‑1 (MQ=255) cAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGt < 1:22036353/68‑1 (MQ=255) aTCAGAGGGGGGAGAAATCCTCCCGCACCCTTGTTCct < 1:6328139/38‑1 (MQ=255) agagGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAACATCAGCGTCaata > 1:24349939/1‑66 (MQ=255) agGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCaagaag > 1:12826258/1‑68 (MQ=255) ggggggAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAgg > 1:28694230/1‑68 (MQ=255) ggggggAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAAGCCGCGACAAGAAgg > 1:22627860/1‑68 (MQ=17) gggggAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGt > 1:13163563/1‑68 (MQ=255) gggggAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGt > 1:27885956/1‑68 (MQ=255) ggggAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAgg < 1:24029691/66‑1 (MQ=255) ggggAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGt < 1:12439267/67‑1 (MQ=255) gagaAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCa > 1:848953/1‑68 (MQ=255) gagaAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCa > 1:10005002/1‑68 (MQ=255) gagaAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCa > 1:16095102/1‑68 (MQ=255) agaAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCa < 1:28903819/67‑1 (MQ=255) agaAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCa < 1:9302202/67‑1 (MQ=255) agaAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCa < 1:24881684/67‑1 (MQ=255) agaAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCa < 1:7081087/67‑1 (MQ=255) agaAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCa < 1:6915508/67‑1 (MQ=255) agaAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCa < 1:6594264/67‑1 (MQ=255) agaAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCa < 1:9355183/67‑1 (MQ=255) aaTCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTaa < 1:19174005/68‑1 (MQ=255) aTCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAac < 1:17893181/68‑1 (MQ=255) aTCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAac < 1:5273695/68‑1 (MQ=255) tcctccCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAaca < 1:16383818/68‑1 (MQ=255) tcctccCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAaca < 1:15103856/68‑1 (MQ=255) tcctccCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAaca < 1:7936205/68‑1 (MQ=255) tcctccCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAac > 1:25607782/1‑67 (MQ=255) tcctccCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAac > 1:5809209/1‑67 (MQ=255) cctccctcACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAacac < 1:28106988/68‑1 (MQ=255) cctccctcACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAacac < 1:2055892/68‑1 (MQ=255) cctccctcACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAacac < 1:13918948/68‑1 (MQ=255) cctccctcACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAacac < 1:3872308/68‑1 (MQ=255) cctccctcACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAacac < 1:284804/68‑1 (MQ=255) ctccctcACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAAcaca < 1:13543792/68‑1 (MQ=255) ctcACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACatat > 1:14496657/1‑67 (MQ=255) ctcACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACatat > 1:3125260/1‑67 (MQ=255) ctcACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATc < 1:5108329/68‑1 (MQ=255) tcACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATc > 1:1141080/1‑67 (MQ=255) tcACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATc > 1:28068435/1‑67 (MQ=255) | ATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTAACACATATC > CP009273/3188149‑3188281 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |