| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 122,580 | T→C | 8.2% | A133A (GCT→GCC) | aceF → | pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 122,580 | 0 | T | C | 8.2% | 551.9 / 14.1 | 184 | A133A (GCT→GCC) | aceF | pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2 |
| Reads supporting (aligned to +/- strand): ref base T (100/62); new base C (3/12); total (104/80) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.24e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 184 total aligned reads displayed.
GGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGC > CP009273/122514‑122646 | ggATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCATGGTGAAAGTTGGCGAAAAAGTTGAAGCTg > 1:647707/1‑68 (MQ=255) gATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCATGGTGAAAGTTGGCGAAAAAGTTGACGCTGa > 1:23528176/1‑68 (MQ=18) atatCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGa > 1:16590280/1‑67 (MQ=255) atatCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGa > 1:5439315/1‑67 (MQ=255) atatCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGa > 1:29485381/1‑67 (MQ=255) tatCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGaa < 1:8374154/67‑1 (MQ=255) atCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACa > 1:8895461/1‑68 (MQ=255) ggCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTc < 1:19462717/68‑1 (MQ=255) ggCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTc > 1:22344078/1‑68 (MQ=255) ggCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTc > 1:4735070/1‑68 (MQ=255) ggCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTCAAAGTTGGCGATAAAGTTGAAGCTGAACAGTc > 1:10920391/1‑68 (MQ=255) gCAGCGACGAAGTTGAAGTGACCGAAATCGTGGTGAAAGTTGGCGATAAAGTTGAAGATGAACAGTCg > 1:2636311/1‑68 (MQ=25) gCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTc > 1:28541468/1‑67 (MQ=255) gCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTc > 1:15535637/1‑67 (MQ=255) gCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTc > 1:3168927/1‑67 (MQ=255) gCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTc > 1:358793/1‑67 (MQ=255) gCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTc > 1:11675801/1‑67 (MQ=255) aGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTc > 1:4333429/1‑65 (MQ=255) aGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTc > 1:19810171/1‑65 (MQ=255) aGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTc > 1:7131939/1‑65 (MQ=255) tgacgaAGTTGAACTGACCGATATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGa < 1:8988610/67‑1 (MQ=18) tgaAGTTGAAATCACCGATATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATc < 1:22688843/66‑1 (MQ=14) cgaAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATc < 1:15920442/67‑1 (MQ=255) cgaAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATc < 1:17890634/67‑1 (MQ=255) cgaAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATc > 1:10023139/1‑67 (MQ=255) aGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCAcc < 1:22913363/67‑1 (MQ=255) aGTTGAACTCACCGAGATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCAcc < 1:26340653/67‑1 (MQ=14) ttGAAGTGACGGAATTCCTGGTGAAAGTGGGCGATAAAGTTGAAGCTGAACAGTCACTGATCACCGt < 1:17429448/67‑1 (MQ=14) ttGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTa > 1:14357830/1‑68 (MQ=255) tGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGt < 1:9615058/66‑1 (MQ=255) tGAACTGACCGAAATCCTGGTGAAAGTTGGCGACAAAGTTGAAGCTGAACAGTCGCTGATCACCGt < 1:5497832/66‑1 (MQ=255) aaGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGaa > 1:13828417/1‑68 (MQ=255) aGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAg > 1:27308261/1‑68 (MQ=255) aGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAg > 1:10093691/1‑68 (MQ=255) aGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAg > 1:10267568/1‑68 (MQ=255) aGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAg > 1:28175932/1‑68 (MQ=255) aGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCCCTGATCACGGTAGAAg > 1:8757528/1‑68 (MQ=255) aGTGACCGAAATCCTGGTGAAAGTTGGCGAGAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAg > 1:7893225/1‑68 (MQ=255) tGACCGAAATCCTGGTGAAAGTTGGCGACAAAGTTGAAGCAGAACAGTCGCTGATCACCGTAGAAGGc > 1:4904958/1‑68 (MQ=14) gACTGAAATGCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCTCTGATCACCGTAGAAGGCg < 1:25461964/68‑1 (MQ=17) gACCGACATCCTGGTGAAAGTTGGCGACAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGc < 1:16685998/67‑1 (MQ=255) gACCGAAATCCTGGTGAAAGTTGTCTATAACGTTGTTTCTGAACAGTCTCTGATCACCGTAGAAGGc < 1:17151738/67‑1 (MQ=18) ccGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGAc > 1:13461456/1‑68 (MQ=255) taTCCTGGTGAAAGTGGGCGATAAAGTTGAAGCGGAACAGTCGCTGATCACCGTAGAAGGCGACAAgg < 1:15025373/67‑1 (MQ=14) aTCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAg > 1:18928108/1‑66 (MQ=255) aTCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGc < 1:16292556/68‑1 (MQ=255) tCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGaa > 1:14005912/1‑56 (MQ=255) tCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCt > 1:13734029/1‑68 (MQ=255) ccTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCt > 1:23092049/1‑67 (MQ=255) ccTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCt > 1:8209942/1‑67 (MQ=255) ccTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCAAAGTAGAATCCGACAAGGCt > 1:12474995/1‑67 (MQ=18) cTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTc < 1:28005012/68‑1 (MQ=255) tGGTCAAAGTTGGCGATAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCt < 1:12894038/68‑1 (MQ=255) ggTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTa < 1:8147738/68‑1 (MQ=255) ggTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTa > 1:15390070/1‑68 (MQ=255) tGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATg > 1:9277837/1‑68 (MQ=255) gAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATgg > 1:7415854/1‑68 (MQ=255) aGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAg > 1:20676608/1‑68 (MQ=255) gTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGt > 1:16365118/1‑68 (MQ=255) gTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGt > 1:10528176/1‑68 (MQ=255) gTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGt > 1:3211702/1‑68 (MQ=255) gTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGt > 1:16900764/1‑68 (MQ=255) tGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGt < 1:4400165/66‑1 (MQ=255) tGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGt < 1:12392783/66‑1 (MQ=255) gggCGATAAAGTTGAAGCGGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGtt < 1:7138478/66‑1 (MQ=255) gggCGACAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGtt < 1:23911895/66‑1 (MQ=255) cGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCg > 1:27948966/1‑67 (MQ=255) caaaGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTc < 1:2490457/67‑1 (MQ=255) caaaGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTc < 1:2613851/67‑1 (MQ=255) aaaGTTGAACCTTAACAGCCGCTCATCCCCGTACACCGCGACAAGGCTTCTATGGAAGTTCCGGCTcc < 1:14260515/68‑1 (MQ=17) aaGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCg < 1:8617852/68‑1 (MQ=255) aaGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCg < 1:13678345/68‑1 (MQ=255) aaGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCg < 1:17353692/68‑1 (MQ=255) aGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGt > 1:25923286/1‑68 (MQ=255) gTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCGTCTATGGAAGTTCCGGCTCCGtt > 1:29083439/1‑68 (MQ=255) ttGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCt < 1:15955869/47‑1 (MQ=255) tGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGtt < 1:4595577/66‑1 (MQ=255) tGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGtt < 1:27381088/66‑1 (MQ=255) tGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTg < 1:28830710/68‑1 (MQ=255) tGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTg < 1:25402349/68‑1 (MQ=255) tGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTg < 1:18562278/68‑1 (MQ=255) tGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTg < 1:21218272/68‑1 (MQ=255) tGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTg < 1:14676697/68‑1 (MQ=255) tGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTg < 1:22143618/68‑1 (MQ=255) tGAAGCTGAACAGTCGCTGATCACCGTAGAAGCCGACAAGGCTTCTAAGGAAGTTCAGGCTCCGtta > 1:21888998/1‑66 (MQ=17) tGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTg < 1:20892214/68‑1 (MQ=255) tGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTg < 1:27691255/68‑1 (MQ=255) tGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCTCCGtat > 1:23230608/1‑65 (MQ=11) tGAAGCAGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTg < 1:13916091/68‑1 (MQ=255) aGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATCGAAGTTCCGGCTCCGTTTGCTg > 1:22018098/1‑68 (MQ=255) gCTGAACAGTCGCTGATCACCGTAGAAGGCGACAATGCTTCTATGGATGTTCCGGCTCCGTTTGCTgg > 1:13974134/1‑68 (MQ=255) gCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGcag > 1:1062717/1‑65 (MQ=255) gCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTg > 1:23853793/1‑67 (MQ=255) gCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTg > 1:14478608/1‑67 (MQ=255) gCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTg > 1:1148172/1‑67 (MQ=255) gCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTg > 1:25262532/1‑67 (MQ=255) gCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTg > 1:26878047/1‑67 (MQ=255) gCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCGCCGTTTGCTg > 1:6059761/1‑67 (MQ=255) gCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCGCCGTTTGCTg > 1:18501231/1‑67 (MQ=255) cTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGc > 1:15218509/1‑68 (MQ=255) | GGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGC > CP009273/122514‑122646 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |