| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,263,460 | C→T | 8.7% | intergenic (+499/+115) | yhaC → / ← rnpB | pentapetide repeats‑related protein/RNase P, M1 RNA component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,263,460 | 0 | C | T | 8.7% | 584.6 / 27.9 | 280 | intergenic (+499/+115) | yhaC/rnpB | pentapetide repeats‑related protein/RNase P, M1 RNA component |
| Reads supporting (aligned to +/- strand): ref base C (152/95); new base T (9/15); total (161/119) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.88e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 288 total aligned reads displayed.
GGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTC > CP009273/3263395‑3263526 | ggACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCa > 1:22591832/1‑68 (MQ=35)ggACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCa > 1:20836286/1‑68 (MQ=35)ggACAGTCATTCATCTTTCTGCCCCTACAAAAGCAAAAACACGCCGAAGCGGGTTTTTACGTAATCCa > 1:26352315/1‑68 (MQ=11) gcggcttatcggtcagtttcacctggtttacgtaaaaacccgcttcggcgggtttttACGTAAACCAg < 1:114926‑M2/11‑1 (MQ=255) aCAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAgg > 1:10026520/1‑68 (MQ=35) aCAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAgg > 1:23467274/1‑68 (MQ=35) aCAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAgg > 1:18592654/1‑68 (MQ=35) aCAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAgg > 1:11119117/1‑68 (MQ=35) aCAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAg < 1:11312693/67‑1 (MQ=35) aCAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAg < 1:25531839/67‑1 (MQ=35) aCAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAg < 1:8933097/67‑1 (MQ=35) aCAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAg < 1:28679424/67‑1 (MQ=35) aCAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAg < 1:13353823/67‑1 (MQ=35) aCAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAg < 1:4671342/67‑1 (MQ=35) aCAGTCATTCATCTTTCGGCCCCTACAAAAGCAAAAACACGCCGACGCGGGTTGTTACGTAATCCAgg > 1:12909337/1‑68 (MQ=11) cattcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGt < 1:6789094/64‑1 (MQ=35) cattcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGaa < 1:17717222/67‑1 (MQ=35) cattcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGaa > 1:29415451/1‑67 (MQ=35) cattcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGa < 1:16125275/66‑1 (MQ=35) catCTTTCTGCCCCTCCAAAGGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGa < 1:17037134/68‑1 (MQ=25) catCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGa < 1:20570679/68‑1 (MQ=35) atCTTTCTGTCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGa > 1:14473906/1‑67 (MQ=25) atCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGa > 1:22206305/1‑67 (MQ=35) atCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGa > 1:22508337/1‑67 (MQ=35) atCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGa > 1:25844254/1‑67 (MQ=35) atCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGa > 1:8133471/1‑67 (MQ=35) atCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGa > 1:11483186/1‑67 (MQ=35) atCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGa > 1:17950729/1‑67 (MQ=35) atCTTTCTGACCCTACAAAAGCAAAAACCCGCCGCAGCCCCTTTTTACGTAAACCAGGTGAAAATGa > 1:6347358/1‑67 (MQ=11) tCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGAcc > 1:14233096/1‑68 (MQ=35) tttCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGa > 1:8756804/1‑68 (MQ=35) ttCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGAt > 1:22832171/1‑68 (MQ=35) gCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGCCCGATAAg > 1:19062770/1‑67 (MQ=25) gCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAg > 1:23981532/1‑67 (MQ=35) gCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAg > 1:27331662/1‑67 (MQ=35) gCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAg > 1:29166263/1‑67 (MQ=35) gCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAg > 1:17601031/1‑67 (MQ=35) gCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAg > 1:16304867/1‑67 (MQ=35) gCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAg > 1:10507544/1‑67 (MQ=35) gCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAg > 1:3209329/1‑67 (MQ=35) gCCCCGCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAg > 1:27553989/1‑67 (MQ=25) cgcccTCCAAAAGCACAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGc < 1:27816396/66‑1 (MQ=255) ccccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGcc > 1:4384944/1‑68 (MQ=35) acctggtttacgtAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGcc < 1:7417970‑M1/55‑1 (MQ=255) cccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGcc > 1:28835675/1‑67 (MQ=35) cccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCg > 1:24231819/1‑68 (MQ=35) cccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCg > 1:9063863/1‑68 (MQ=35) acTCAAAAATCACAAACCCGCCGCATCGGGTTTTTACGTAAACCAGGTCAAACTGACCGATAAGCCGc < 1:10067529/67‑1 (MQ=11) aaaaGCACAAACCCGCCGCATCGGTTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGctttct < 1:25738062/68‑1 (MQ=11) aaaaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGctttct < 1:25428505/68‑1 (MQ=255) aaaaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGctttct < 1:19342417/68‑1 (MQ=255) aaaaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGctttct < 1:20717627/68‑1 (MQ=255) aaaaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGctttct < 1:19586257/68‑1 (MQ=255) aaaaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGctttct < 1:7184074/68‑1 (MQ=255) aGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGcgttctttg > 1:11774553/1‑67 (MQ=255) aGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGGTTTCtttt > 1:5837575/1‑68 (MQ=255) aGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCtttt > 1:11945760/1‑68 (MQ=40) aGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCtgtt > 1:23603434/1‑68 (MQ=255) aGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCGTTCtttt > 1:26614591/1‑68 (MQ=255) cttAAACCCGCCGCATCGGGTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTgg > 1:14809313‑M1/4‑68 (MQ=255) ctgAAACCCGCCGTACCGGTTTTTTACGTAAACCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTgg > 1:3456062‑M1/4‑68 (MQ=255) catAAACCCGCCGTATCGGTTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTgg > 1:10373874‑M1/4‑68 (MQ=255) atAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTggg > 1:7298719‑M1/3‑68 (MQ=255) AAAAACCCGCCGTAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTgg > 1:2607181‑M1/1‑67 (MQ=255) AAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTgg > 1:1989670‑M1/1‑67 (MQ=255) tAAACCCGCCGTATCGGTTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGt > 1:853509‑M1/2‑68 (MQ=255) tAAACCCGCCGTACCGGGTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGt > 1:7904357‑M1/2‑68 (MQ=255) tAAACCCGCCGCAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGt > 1:124726‑M1/2‑68 (MQ=255) AAACCCCGCCGACCCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGt > 1:29250744‑M1/1‑68 (MQ=255) AAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGt > 1:9635431‑M1/1‑68 (MQ=255) AAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACGGACCGATAAGCCGCTTTCTTTTGGGt > 1:6125208‑M1/1‑68 (MQ=255) AACCCGCCGCATCGGTTTTTTACGTAAACCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGt > 1:5125228‑M1/1‑66 (MQ=255) ACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGtata < 1:2958551‑M1/68‑1 (MQ=255) CCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGGTTTCTTTTGGGtata < 1:18205089‑M1/67‑1 (MQ=255) CCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGtata < 1:29064917‑M1/67‑1 (MQ=255) CCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTGGggtaga < 1:7621694‑M1/67‑3 (MQ=255) GCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGt > 1:14032461‑M1/1‑68 (MQ=255) GCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGt > 1:5524276‑M1/1‑68 (MQ=255) aaGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTg > 1:1583999/1‑68 (MQ=40) atcgggttattACGTAAACCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTgg < 1:10888405‑M2/57‑1 (MQ=255) tttttACGTACACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGt < 1:917982‑M2/62‑1 (MQ=255) tttttACGTACACCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGt < 1:15649793‑M2/62‑1 (MQ=255) tttttACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTc > 1:4492243‑M2/6‑68 (MQ=255) tttttACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTc < 1:28306070‑M2/63‑1 (MQ=255) tttttACGTAAACCAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGt < 1:6564820‑M2/62‑1 (MQ=255) tttttACGTAAACCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTc < 1:23796425‑M2/63‑1 (MQ=255) ttttACGTAAACCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTc < 1:24510842‑M2/63‑1 (MQ=255) ttttACGTAAACCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTc < 1:17611018‑M2/63‑1 (MQ=255) tttACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTAGTGGAAAGTcaa > 1:8232210‑M2/4‑67 (MQ=255) tttACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCAt < 1:23123005‑M2/65‑1 (MQ=255) tACGTAAATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTc < 1:24400539‑M2/67‑1 (MQ=255) aCGTAAGCCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGAGTCGTGGACAGtcattca > 1:21534454/1‑68 (MQ=255) aCGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGtcattca > 1:15582245/1‑68 (MQ=255) aCGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtcattca > 1:4266875/1‑68 (MQ=40) gTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtcattcatg > 1:3984570/1‑67 (MQ=255) gTAAACCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATc < 1:1966114/68‑1 (MQ=255) gTAAACCAGCTGAAACTGACCGATAAGCCGCTTTCTTTTTGGTATAGCGTCGTGGACAGTCATTCATc < 1:17207301/68‑1 (MQ=14) aaaTCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCtt < 1:2196647/68‑1 (MQ=255) aaaTCAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCtt < 1:25996270/68‑1 (MQ=255) aCCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCtt > 1:20746379/1‑66 (MQ=39) | GGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTC > CP009273/3263395‑3263526 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |