| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,324,911 | T→G | 8.0% | E68A (GAG→GCG) | obgE ← | GTPase involved in cell partioning and DNA repair |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,324,911 | 0 | T | G | 8.0% | 848.5 / 11.0 | 322 | E68A (GAG→GCG) | obgE | GTPase involved in cell partioning and DNA repair |
| Reads supporting (aligned to +/- strand): ref base T (167/124); new base G (28/2); total (196/126) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.78e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 342 total aligned reads displayed.
CTTTAATCGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCGGCTTGCGCCATTCT‑‑GACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAGCGTGTTCAGGTTCTCGTCGGCTTCCATCC > CP009273/3324846‑3324977 | cTTTAATCGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCGGCTTGCGCCATTCT‑‑GACCGCGctct < 1:8082386/68‑1 (MQ=255)cTTTAATCGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCGGCTTGCGCCATTCT‑‑GACCGCGctct < 1:16708194/68‑1 (MQ=255)cTTTAATCGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCGGCTTGCGCCATTCT‑‑GACCGCGctct < 1:16002914/68‑1 (MQ=255)cTTTAATCGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCGGCTTGCGCCATTCT‑‑GACCGCGctct < 1:25054611/68‑1 (MQ=255)cTTTAATCGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCGGCTTGCGCCATTCT‑‑GACCGCGctct < 1:12358270/68‑1 (MQ=255) ttAATCGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCGGCTTGCGCCATTCT‑‑GACCGCGCTCTGc < 1:5055975/68‑1 (MQ=255) aaTCGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCGGGTTGCGCCCTTAT‑‑GCCCCCGCGCTggcc > 1:3730316/1‑65 (MQ=255) cGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCGGGTTGCGCCATTCT‑‑GACCGCGCTCTGGACGGa > 1:1374130/1‑68 (MQ=255) cGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCGGCTTGCGCCATTCT‑‑GACCGCGCTGTGCACGGa > 1:6804853/1‑68 (MQ=255) cGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCGGCTTGCGCCATTCT‑‑GACCGCGCTCTGCACggt > 1:26700081/1‑67 (MQ=255) cGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCGGCTTGCGCCATTCT‑‑GACCGCGCTCTGCACGGa > 1:28588168/1‑68 (MQ=255) cGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCGGCTTGCGCCATTCT‑‑GACCGCGCTCTGCACGGa < 1:27968662/68‑1 (MQ=255) cGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCGGCTTGCGCCATTAT‑‑GCCCGCGGGCTGCCCggc > 1:15291886/1‑67 (MQ=255) cGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCGGCGTGCGCCCGTAT‑‑GTCCGCGCGCTGGCCGGa > 1:17361382/1‑68 (MQ=255) tCTTTACCGCGCTTACCGGTACAGTCGCGGCTTGCGGCATTCT‑‑GACCGCGCTCTGGACCGAAAGAttt > 1:11542643/1‑68 (MQ=255) cTTTACCGCGCTTACCGGTACAGTCGCGGCTTGCGCCATTCT‑‑GACCGCGCTCTGGACGGAAAGAtttt > 1:24297363/1‑68 (MQ=255) aCCGCGCTTACCGGTACAGTCGCGGGTTGCGCCATTCT‑‑GACCGCGCTCTGCACGGAAAGATTTTTGaa > 1:19227063/1‑68 (MQ=255) aCCGCGCTTACCGGTACAGTCGCGGCTTTGGCCCTTAT‑‑TACCCCGCGGTGCACGGGAAGATTTTTGaa > 1:13192040/1‑68 (MQ=255) aCCGCGCTTACCGGTACAGTCGCGGCTTGGGCCATTCT‑‑GGCCGCGCGCTGCACGGGAAGATTTTTaaa > 1:3422204/1‑68 (MQ=255) aCCGCGCTTACCGGTACAGTCGCGGCTTGCGCCATTCT‑‑GCCCGCGCTCTGCACGGAAAGATTTTTCaa > 1:19032088/1‑68 (MQ=255) aCCGCGCTTACCGGTACAGTCGCGGCTTGCGCCATTCT‑‑GACCGCGCTCTGCACGGAAAGATTTTTCaa > 1:20331655/1‑68 (MQ=255) aCCGCGCTTACCGGTACAGTCGCGGCTTGCGCCATTCT‑‑GACCGCGCTCTGCACGGAAAGATTTTTCaa > 1:1968998/1‑68 (MQ=255) aCCGCGCTTACCGGTACAGTCGCGGCTTGCGCCATTCT‑‑GACCGCGCTCTGCACGGAAAGATTTTTCaa > 1:2226888/1‑68 (MQ=255) aCCGCGCTTACCGGTACAGTCGCGGCTTGCGCCATTCT‑‑GACCGCGCGCAGGCCGGAAAGATTTTTCaa > 1:13492898/1‑68 (MQ=255) aCCGCGCTTACCGGTACAGTCGCGGCTTGCGCCATTAT‑‑GACCGCGCTCTGCACGGAAAGATTTTTCaa > 1:5990977/1‑68 (MQ=255) aCCGCGCTTACCGGTACAGTCGCGGCGTTCGCCAGTCTTAAACCGCGCT‑TACA‑GGAAAGATTTTTaaa > 1:19942157/1‑68 (MQ=255) aCCGCGCTTACCGGTACAGTCGCGGCGTGCGCCCTTCT‑‑GCCCGCGCGCTGCCCGGAAAGATTTTTcta > 1:29171250/1‑66 (MQ=255) cgcgCTTACCGGTACAGTCGCGGGTTGGGCCCGTAT‑‑GACCCCCCGCGGCCCCGTAAGAGTTTTGaaa > 1:6632568/1‑67 (MQ=255) cgcgCTTACCGGTACAGTCGCGGCTTGGGCCATTGT‑‑GACCGCGCTCTGGACGGAAAGATTTTTGaaa > 1:5318779/1‑67 (MQ=255) cgcgCTTACCGGTACAGTCGCGGCTTGCGCCATTCT‑‑GACCGCGCTCTGCACGGAAAGATTTTTCaaaa > 1:18088139/1‑68 (MQ=255) cgcgCTTACCGGTACAGTCGCGGCTTGCGCCATTCT‑‑GACCGCGCTCTGCACGGAAAGATTTTTCaaa > 1:12007936/1‑67 (MQ=255) cgcgCTTACCGGTACAGTCGCGGCTTGCGCCAATCT‑‑GACCGCGCTCGGCACGGAAAGATTTTTGaaa > 1:26283426/1‑67 (MQ=255) gcTTACCGGTACAGTCGCGGCTTGCGCCATTCT‑‑GACCGCGCTCTGCACGGAAAGATTTTTCAAAACg < 1:27869847/67‑1 (MQ=255) gcTTACCGGTACAGTCGCGGCTTGCGCCATTCT‑‑GACCGCGCTCTGCACGGAAAGATTTTTCAAAACg < 1:7593529/67‑1 (MQ=255) ttACCGGTACAGTCGCGGCTTGCGCCATTCT‑‑GACCGCGCTCTGCACGGAAAGATTTTTCAAAACGAt < 1:27286254/67‑1 (MQ=255) ttACCGGTACAGTCGCGGCTTGCGCCATTCT‑‑GACCGCGCTCTGCACGGAAAGATTTTTCAAAACGAt < 1:27666059/67‑1 (MQ=255) aCCGGTACTGTCGCGGCTTGCGCCATTCT‑‑GACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAAt < 1:29722407/68‑1 (MQ=255) aCCGGTACAGTCGCGGCTTGCGCCATTCT‑‑GACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAAt < 1:2934816/68‑1 (MQ=255) aCCGGTACAGTCGCGGCTTGCGCCATTCT‑‑GACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAAt < 1:17692565/68‑1 (MQ=255) ccGGTACAGTCGCGGCTTGCGCCATTCT‑‑GACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATc > 1:4978543/1‑68 (MQ=255) cGGTACAGTCGCGGCTTGCGCCATTCT‑‑GACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCg < 1:18433961/68‑1 (MQ=255) cGGTACAGTCGCGGCTTGCGCCATTCT‑‑GACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCg < 1:19478081/68‑1 (MQ=255) gTACAGTCGCGGCTTGCGCCATTCT‑‑GACCGCGCTCTGCACGGCAAGATTTTTGAAAACGATAATAGAt > 1:940604/1‑68 (MQ=255) tACAGTCGCGGCTTGCGCCATTCT‑‑GACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGAt < 1:21540504/67‑1 (MQ=255) aCAGTCGCGGCTTGCGCCATTCT‑‑GACCGCGCTCTGGACGGAAAGATTTTTCAAAACGATAATCGATaa > 1:14152029/1‑68 (MQ=255) aCAGTCGCGGCTTGCGCCATTCT‑‑GACCGCGCTCTGCACGGTAAGATTTTTAAAAACGATAATCGATaa > 1:18710235/1‑68 (MQ=255) aCAGTCGCGGCTTGCGCCATTCT‑‑GACCGCGCTCTGCACGGAAAGATTTTTGAAAACGATAATCGATaa > 1:26824562/1‑68 (MQ=255) aCAGTCGCGGCTTGCGCCATTCT‑‑GACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATaa > 1:11386297/1‑68 (MQ=255) aCAGTCGCGGCTTGCGCCATTCT‑‑GACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATaa > 1:18999706/1‑68 (MQ=255) aCAGTCGCGGCTTGCGCCATTCT‑‑GACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATaa > 1:18928665/1‑68 (MQ=255) aCAGTCGCGGCTTGCGCCATTCT‑‑GACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATaa > 1:11753588/1‑68 (MQ=255) aCAGTCGCGGCTTGCGCCATTCT‑‑GACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATaa > 1:6249468/1‑68 (MQ=255) aCAGTCGCGGCTTGCGCCATTCT‑‑GACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATaa > 1:5670702/1‑68 (MQ=255) aCAGTCGCGGCTTGCGCCATTCT‑‑GACCGCGCTCTGCACGGAAAGATTTTTAAAAACGATTATCGATaa > 1:20783543/1‑68 (MQ=255) aCAGTCGCGGCTTGCGCCATTCT‑‑GACCGCGCTCTGCACGGAAAGATTTTTAAAAACGATAATCGATaa > 1:28097991/1‑68 (MQ=255) aCAGTCGCGGCTTGCGCCATTCT‑‑GACCGCGCTCTGCACGGAAAGATTTTTAAAAACGATAATCGATaa > 1:4788619/1‑68 (MQ=255) aCAGTCGCGGCTTGCGCCATTAT‑‑GACCGCGCGCTGCACGGAAAGATTTTTAAAAAAGATAATCGATaa > 1:18558191/1‑68 (MQ=255) gTCGCGGCTTGCGCCATTCT‑‑GACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAg < 1:12609470/66‑1 (MQ=255) cgcgGCTTGCGCCATTCT‑‑GACGGCGCTCTGCAGGGAAAGATTTTTCAAAACCATTATCGATAAGCgt < 1:7422838/67‑1 (MQ=255) cgcgGCTTGCGCCATTCT‑‑GACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAg < 1:3917701/64‑1 (MQ=255) cgcgGCTTGCGCCATTCT‑‑GACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAg < 1:17170169/64‑1 (MQ=255) cgcgGCTTGCGCCATTCT‑‑GACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAGc < 1:8870876/65‑1 (MQ=255) cgcgGCTTGCGCCATTCT‑‑GACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAGCgt < 1:11464134/67‑1 (MQ=255) ggCTTGCGCCATTCT‑‑GACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAGCGTGtt < 1:23816123/67‑1 (MQ=255) ggCTTGCGCCATTCT‑‑GACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAGCGTGTTc > 1:11018186/1‑68 (MQ=255) cTTGCGCCATTCT‑‑GACCGCGCTCTGCCCGGAAAGATTTTTCAAAACGATAATCGATAAGCGTGTTCAg > 1:1562605/1‑68 (MQ=255) cTTGCGCCATTCT‑‑GACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAGCGTGTTCAg > 1:13045780/1‑68 (MQ=255) cTTGCGCCATTCT‑‑GACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAGCGTGTTCAg > 1:4167829/1‑68 (MQ=255) ttGCGCCATTCT‑‑GACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAGCGTGTTCAgg < 1:10745799/68‑1 (MQ=255) tGCGCCATTCT‑‑GACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAGCGTGTTCAGGt < 1:18277830/68‑1 (MQ=255) tGCGCCATTCT‑‑GACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAGCGTGTTCAGGt < 1:28452085/68‑1 (MQ=255) tGCGCCATTCT‑‑GACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAGCGTGTTCAGGt < 1:4475288/68‑1 (MQ=255) gcCATTCT‑‑GACGGCGCGCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAGCGTGTTCAGGTtct < 1:25859905/68‑1 (MQ=255) gcCATTCT‑‑GACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAGCGTGTTCAGGTtct < 1:13925068/68‑1 (MQ=255) ccATTCT‑‑GACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAGCGTGTTCAGGTtctc > 1:20400905/1‑68 (MQ=255) tCT‑‑GACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAGCGTGTTCAGGTTCtcgtcg > 1:25539851/1‑68 (MQ=255) tCT‑‑GACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAGCGTGTTCAGGTTCtcgtcg > 1:20877173/1‑68 (MQ=255) tCT‑‑GACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAGCGTGTTCAGGTTCtcgtcg > 1:6446149/1‑68 (MQ=255) t‑‑GACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAGCGTGTTCAGGTTCTCGTCGGc < 1:24809335/68‑1 (MQ=255) t‑‑GACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAGCGTGTTCAGGTTCTCGTCGGc < 1:2002847/68‑1 (MQ=255) gACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAGCGTGTTCAGGTTCTCGTCGGCt > 1:24436179/1‑68 (MQ=255) gACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAGCGTGTTCAGGTTCTCGTCGGCt > 1:12202008/1‑68 (MQ=255) aCCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAGCGTGTTCAGGTTCTCGTCGGCt > 1:24208883/1‑67 (MQ=255) ccGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAGCGTGTTCAGGTTCTCGTCGGCtt > 1:2601226/1‑67 (MQ=255) ccGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAGCGTGTTCAGGTTCTCGTCGGCtt > 1:792870/1‑67 (MQ=255) ccGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAGCGTGTTCAGGTTCTCGTCGGCtt > 1:24021969/1‑67 (MQ=255) ccGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAGCGTGTTCAGGTTCTCGTCGGCtt > 1:24580732/1‑67 (MQ=255) ccGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAGCGTGTTCAGGTTCTCGTCGGCtt > 1:909480/1‑67 (MQ=255) ccGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAGCGTGTTCAGGTTCTCGTCGGCtt > 1:12840410/1‑67 (MQ=255) ccGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAGCGTGTTCAGGTTCTCGTCGGCtt > 1:8687682/1‑67 (MQ=255) ccGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAGCGTGTTCAGGTTCTCGTCGGCtt > 1:3182965/1‑67 (MQ=255) ccGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAGCGTGTTCAGGTGCTCGTCGGCtt > 1:21408283/1‑67 (MQ=255) gcgcTCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAGCGTGTTCAGGTTCTCGTCGGCTtcca > 1:14293463/1‑68 (MQ=255) gcgcTCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAGCGTGTTCAGGTTCTCGTCGGCTtcca < 1:20591884/68‑1 (MQ=255) cgcTCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAGCGTGTTCAGGTTCTCGTCGGCTtcca > 1:29528352/1‑67 (MQ=255) gcTCTGCTCGGAAAGATTTTTCAAAACGATAATCGATAAGCGTGTTCAGTTTCTCGTCGGCTtcaat < 1:14596957/67‑1 (MQ=255) gcTCTGCACGGAAAGATTTTTCAAGACGATAATCGATAAGCGTGTTCAGGTTCTCGTCGGCTtccat < 1:10464830/67‑1 (MQ=255) gcTCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAGCGTGTTCAGGTTCTCGTCGGCTtccat < 1:2202931/67‑1 (MQ=255) gcTCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAGCGTGTTCAGGTTCTCGTCGGCTtccat < 1:10295086/67‑1 (MQ=255) gcTCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAGCGTGTTCAGGTTCTCGTCGGCTtccat < 1:9506609/67‑1 (MQ=255) | CTTTAATCGTCACGTCTTTACCGCGCTTACCGGTACAGTCGCGGCTTGCGCCATTCT‑‑GACCGCGCTCTGCACGGAAAGATTTTTCAAAACGATAATCGATAAGCGTGTTCAGGTTCTCGTCGGCTTCCATCC > CP009273/3324846‑3324977 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |