| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,462,400 | A→G | 5.5% | S271S (AGT→AGC) | chiA ← | periplasmic endochitinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,462,400 | 0 | A | G | 5.5% | 1136.5 / 21.8 | 364 | S271S (AGT→AGC) | chiA | periplasmic endochitinase |
| Reads supporting (aligned to +/- strand): ref base A (179/154); new base G (14/6); total (204/160) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.73e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 366 total aligned reads displayed.
AGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATA > CP009273/3462336‑3462465 | aGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTTCTg > 1:5755955/1‑68 (MQ=255)aGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTTCTg > 1:25408629/1‑68 (MQ=255)aGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTGCTg > 1:17902432/1‑68 (MQ=255)aGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTg > 1:23388287/1‑68 (MQ=255)aGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTg > 1:19702623/1‑68 (MQ=255)aGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTg > 1:21937751/1‑68 (MQ=255) ccAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTTCTGGc > 1:1491034/1‑68 (MQ=255) cAGGTTTACCATACTGGCTGATTTCTGTTGCCGTTGCGGCTCGAACATAACGCCATGGGTTGCTGGCa < 1:24117455/68‑1 (MQ=18) cAGGGTTACCATACTGGGTGATTTCTGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTgg < 1:1713503/66‑1 (MQ=14) cAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTgg < 1:19468244/66‑1 (MQ=255) gggTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTGCTGGCATc > 1:7425879/1‑68 (MQ=255) gggTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATc > 1:2978249/1‑68 (MQ=255) gggTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATc > 1:3655122/1‑68 (MQ=255) ggTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATATCGCCATGGGTTGCTGGCATct > 1:20189429/1‑67 (MQ=25) ggTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTGCTGGCATca > 1:29178979/1‑67 (MQ=255) gTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCTCGAACATAACGCCATGGGTTGCTGGCATc < 1:27946846/66‑1 (MQ=255) gTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATcat > 1:27352109/1‑66 (MQ=255) gTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATcat > 1:12347632/1‑66 (MQ=255) gTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGt > 1:11577412/1‑68 (MQ=255) gTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGt > 1:12885362/1‑68 (MQ=255) ttACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGt < 1:5488306/67‑1 (MQ=255) ttACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGt < 1:1604561/67‑1 (MQ=255) ttACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGt < 1:18313532/67‑1 (MQ=255) cATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTTCTGGCATCAtttagt > 1:24169678/1‑65 (MQ=21) cATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGtttagt > 1:28793245/1‑65 (MQ=255) cATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttct < 1:9103105/68‑1 (MQ=255) cATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttct > 1:23248512/1‑68 (MQ=255) cATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttct > 1:21392931/1‑68 (MQ=255) cATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttct > 1:16953598/1‑68 (MQ=255) cATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCAtttagt > 1:8636020/1‑65 (MQ=255) aTACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttctt > 1:17792067/1‑68 (MQ=255) aTACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttctt > 1:28170488/1‑68 (MQ=255) aTACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCATtttatt > 1:15558124/1‑68 (MQ=255) tACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttctt > 1:24675962/1‑67 (MQ=255) tACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttctt > 1:2295304/1‑67 (MQ=255) tACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttctt > 1:25558723/1‑67 (MQ=255) tACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttctt > 1:6984928/1‑67 (MQ=255) tACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttctt > 1:10323139/1‑67 (MQ=255) tACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttctt > 1:1848202/1‑67 (MQ=255) aCTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTCCTGGCATCATttgttttc > 1:29341359/1‑68 (MQ=18) aCTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttctttc > 1:11139534/1‑68 (MQ=255) aCTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttctttc > 1:16561886/1‑68 (MQ=255) aCTGGCTGATTTCTGTTGCAGTTGCAGCGCGAACATAACGCCATGGGTTACTGGCATCATttagtttc > 1:21315412/1‑68 (MQ=18) ggCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCa > 1:24505838/1‑68 (MQ=255) cTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATc > 1:4434732/1‑68 (MQ=255) cTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATc > 1:22568046/1‑68 (MQ=255) tGATTTCTGTTGCCGTTGCCGCGCGAACATCACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCg < 1:19044116/68‑1 (MQ=255) tGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCg < 1:23632749/68‑1 (MQ=255) tGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCg < 1:9377947/68‑1 (MQ=255) gATTTCTGTTGCCGTTGGCGCGCGAACATAACGCCTTGTGTTAGTGGCATGGTTTTCTTTCGCTTCgg < 1:19948265/68‑2 (MQ=25) tttCTGTTGCCGGTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCt < 1:3962933/68‑1 (MQ=255) ttCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTg > 1:6424260/1‑68 (MQ=255) ttCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTGCTTTCGCATCGccgg > 1:26738460/1‑66 (MQ=255) tCTGTTGCCGTTGCCGCGCGAACTTAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTgg < 1:12273782/68‑1 (MQ=255) tCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTgg < 1:27754458/68‑1 (MQ=255) tGTTGCCGTTGCCGCGCTAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGc < 1:20670404/68‑1 (MQ=255) gttgccgttgccGCGCGAACATAACGCCATGGGTTTCTGGCATCGTTGTATTTCGCATCGCCAGGGCa > 1:764482/1‑68 (MQ=18) gttgccgttgccGCGCGAACATAACGCCATGGGTTACTGGCATCATTTTATTTCGCATCCCCTGGGCa > 1:10007608/1‑68 (MQ=21) cgttgccgCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTGTTTCGCATCGCCAGGCCATTTTg > 1:17394359/1‑68 (MQ=25) cgttgccgCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAAttt > 1:13169024/1‑67 (MQ=255) cgttgccgCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAAttt > 1:10785531/1‑67 (MQ=255) gccgCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCcgc > 1:28516140/1‑68 (MQ=255) cgcgcgAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCcgcg < 1:28936202/67‑1 (MQ=255) cgcgcgAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCcgcg < 1:11963678/67‑1 (MQ=255) cgcgcgAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCcgcg < 1:26108010/67‑1 (MQ=255) cgcgcgAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCcgcg < 1:22345232/67‑1 (MQ=255) cgcgcgAACATAACGCCATGGGTTACTGGCATCGTTGAGTTTCGCATCGCCAGGGCAGTTAGCagcg > 1:961521/1‑67 (MQ=14) gcgcgAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGcc < 1:8077486/68‑1 (MQ=255) gAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTAcc < 1:3440278/68‑1 (MQ=255) aCATAACGCCATGGGTTACTTGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACcca < 1:29492038/68‑1 (MQ=255) aCATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCTTCGCCTGGGCAATTTGCCGCGCCTACcca < 1:9793632/68‑1 (MQ=255) aCATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACcca < 1:7359860/68‑1 (MQ=255) aCATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACcca < 1:6535041/68‑1 (MQ=255) aCATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACcca < 1:8405765/68‑1 (MQ=255) aCATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACcca < 1:5967845/68‑1 (MQ=255) cATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCTCCAGGGCAATTTGAAGAGGCTACcca > 1:147033/1‑67 (MQ=17) cGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGc > 1:11767672/1‑68 (MQ=255) cGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGc > 1:27494505/1‑68 (MQ=255) cGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGc < 1:19129482/68‑1 (MQ=255) gCCATGGGTTACTTGCATACTTTTATTACGAATCCCCAGGCCAATTTTCAGCGCCTACCCACCAGGCg > 1:26856304/1‑68 (MQ=255) ccATGGGTTACTGGCATCTTTTTATTTCGCATCTCCAGGGCAATTTGAAGAGGCTACCCACCAGGcat > 1:14038184/1‑66 (MQ=2) ccATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCAGGGCAGTTTGAAGAGCCTACCCACCAGGcat > 1:8775246/1‑66 (MQ=17) cATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGt < 1:15919163/67‑1 (MQ=255) cATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGt < 1:26443359/67‑1 (MQ=255) cATGGGTTACTGGCATCGTTTTCTATCGCACCGCCTGGGCAATTTGACGCGCCTACCCACCAGGCGtt < 1:20819069/68‑1 (MQ=38) cATGGGTTACTGGATTATTAATCTTTCGCATCGCCTGGGCAATTTTACGAGCCTACCCACCAGGCGtt < 1:14414928/68‑1 (MQ=255) tGGGTTACTGGCTTAGTTTTCTATCGCACCGCCTGGGCAATTTTACGAGCCTACCCACCAGGCGTTCt < 1:25824748/68‑1 (MQ=25) tGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCt < 1:26164055/68‑1 (MQ=255) tGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCt < 1:5354971/68‑1 (MQ=255) tGGGTTACTGGCATCGGTTTCTTTCGCAGCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCt < 1:4808459/68‑1 (MQ=255) tGGGTTACTGGACTAGTCTTCTATCGCAGCGCCTGGGCAATATTCCGCGCCTACCCACCAGGCGTTCt < 1:24966193/68‑1 (MQ=255) tGGGTTAATGGAATCGTGTTCTATCGCATCGCGTGGGCAATTTTTCGCGCCTACCCACCAGGCGTTCt < 1:13529821/68‑1 (MQ=25) gggTTACTGGCATCGTTTTCTTTCGCATCGCCGGGGCAATTTGAAGCCGCTACCCACCAGGCATtttt > 1:28240160/1‑68 (MQ=18) ggTTACTGGCATCGTTTTCTTTCGCATCGCCTggg > 1:21014811/1‑35 (MQ=255) ggTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTta > 1:18522684/1‑68 (MQ=255) ggTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTta > 1:12574418/1‑68 (MQ=255) gTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTta < 1:15238721/67‑1 (MQ=255) gTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTta < 1:6218236/67‑1 (MQ=255) gTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTta < 1:15091272/67‑1 (MQ=255) gTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTta < 1:18852931/67‑1 (MQ=255) | AGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATA > CP009273/3462336‑3462465 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |