| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,462,438 | C→A | 9.3% | A259S (GCA→TCA) | chiA ← | periplasmic endochitinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,462,438 | 0 | C | A | 9.3% | 1517.7 / 24.7 | 528 | A259S (GCA→TCA) | chiA | periplasmic endochitinase |
| Reads supporting (aligned to +/- strand): ref base C (100/359); new base A (12/35); total (113/415) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.81e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 531 total aligned reads displayed.
TTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAAC > CP009273/3462372‑3462503 | cgcgcgAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCcgcg < 1:10412117/67‑1 (MQ=255) cgcgcgAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCcgcg < 1:6500713/67‑1 (MQ=255) cgcgcgAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCcgcg < 1:26108010/67‑1 (MQ=255) gcgcgAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGcc < 1:15926444/68‑1 (MQ=255) aCATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACcca < 1:8405765/68‑1 (MQ=255) aCATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACcca < 1:7359860/68‑1 (MQ=255) cGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGc > 1:9894150/1‑68 (MQ=255) cGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGc < 1:19129482/68‑1 (MQ=255) gCCATGGGTTACTTGCATACTTTTATTACGAATCCCCAGGCCAATTTTCAGCGCCTACCCACCAGGCg > 1:26856304/1‑68 (MQ=255) gCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCg > 1:1117030/1‑68 (MQ=255) ccATGGGTTACTGGCATCTTTTTATTTCGCATCTCCAGGGCAATTTGAAGAGGCTACCCACCAGGcat > 1:14038184/1‑66 (MQ=2) ccATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGt > 1:12928377/1‑68 (MQ=255) ccATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGt > 1:8120999/1‑68 (MQ=255) cATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGtt < 1:26507357/68‑1 (MQ=255) cATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGtt < 1:23577752/68‑1 (MQ=255) cATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGt < 1:26687197/67‑1 (MQ=255) cATGGGTTACTGGATTCGTTTTCTATCGCAGCGCGTGGGCAATTTGCCGCGCCTACCCACCAGGCGtt < 1:3484257/68‑1 (MQ=255) tGGGTTACTGGCATCGGTTTCTTTCGCAGCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCt < 1:4808459/68‑1 (MQ=255) tGGGTTACTGGACTAGTCTTCTATCGCAGCGCCTGGGCAATATTCCGCGCCTACCCACCAGGCGTTCt < 1:24966193/68‑1 (MQ=255) gggTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCtt > 1:9001010/1‑68 (MQ=255) gggTTACTGGCATCGTTTTCTTTCGCATCGCCGGGGCAATTTGTCGCGCCTACCCACCAGGCCttttg > 1:16037775/1‑65 (MQ=37) gTTACTGGCATCGTTTTCTTTCGCATCGCATGGGCAATTTGCCGCGCCTACCCACCAGGCCTtattat > 1:12188235/1‑68 (MQ=255) aCTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTATtatata > 1:15002785/1‑66 (MQ=255) tGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCCCCAGGCGTTATTATAAAt > 1:24841540/1‑67 (MQ=255) tGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAAt > 1:25092795/1‑67 (MQ=255) tGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAAt > 1:22800055/1‑67 (MQ=255) tGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTATTATAAAt > 1:5388881/1‑67 (MQ=255) tGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCATTTTtata > 1:22654763/1‑64 (MQ=255) ggCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTGTTATAAATg > 1:1943113/1‑67 (MQ=255) ggCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATg > 1:6731838/1‑67 (MQ=255) tCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATgccgcc < 1:4400305/68‑1 (MQ=255) tttttCTTTCGGATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:10055629/67‑1 (MQ=255) tttttCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:15185219/67‑1 (MQ=255) tttttCTTTCGCATCCCATGGGCAATTTTGCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:29388113/67‑1 (MQ=37) tttttCTATCGCATCCCCTGGGCAATATTCAGAGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:509510/67‑1 (MQ=21) gtgttctttcGCAGCGCCTGGGCAATTTTCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:2895548/65‑1 (MQ=38) gtcttctttgGTATCGGGTGGGCAATATTTAGAGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:885357/65‑1 (MQ=9) gtattctttcGCATCGCCTGGGCAATGTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:9291024/65‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:24142270/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:3056143/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:27793672/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:14642312/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:15891953/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:1244893/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:1760179/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:10632225/68‑1 (MQ=255) gTTTTCTTTCGCAGCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:16212106/68‑1 (MQ=255) ttttCTATGGCACCGCCTGGGCAATTTTCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:3766223/67‑1 (MQ=38) ttcttttGCATCGCCTGGGCAATTTGCCGCGGCTACCCACCAGGCGTTCTTATAAATGCCGCCGTtat < 1:8010316/68‑1 (MQ=255) ttctttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTtat < 1:15678539/68‑1 (MQ=255) ttctttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTtat < 1:11655681/68‑1 (MQ=255) ttctttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTtat < 1:27571693/68‑1 (MQ=255) ttctttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTtat < 1:7069358/68‑1 (MQ=255) ttctttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTtat < 1:2455856/68‑1 (MQ=255) tctttcGCACCGCGTGGGCAATTTTCAGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTtat < 1:12667349/67‑1 (MQ=37) ctttgGGATCCCCTGGGCAATTTTACGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAg < 1:20568954/68‑1 (MQ=37) ttggCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:18576024/65‑1 (MQ=255) ttggCATCGCCTGGGCAATATTTAGAGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:19662668/65‑1 (MQ=21) ttcGCATCGCCTGGGCAATTTTCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:21821800/68‑1 (MQ=255) ttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt > 1:9449972/1‑68 (MQ=255) ttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt > 1:6046576/1‑68 (MQ=255) ttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:8635470/68‑1 (MQ=255) ttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:12301627/68‑1 (MQ=255) ttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:20057864/68‑1 (MQ=255) ttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:22272034/68‑1 (MQ=255) ttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTATTATAAATGGCGCCGTTATAGAt > 1:14227449/1‑68 (MQ=255) ttcGCATCCCCTGGGCAATTTTAAGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:28360134/68‑1 (MQ=37) ttcGCAACCCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:24374808/68‑1 (MQ=255) ctgaTCGCCTCGGCAATTTGCCGCGCATACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATaa < 1:16796404/65‑1 (MQ=39) gCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAAc < 1:25931266/68‑1 (MQ=255) gCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAAc < 1:5559237/68‑1 (MQ=255) gCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAAc < 1:21141681/68‑1 (MQ=255) gCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAAc < 1:18340710/68‑1 (MQ=255) aTCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGt < 1:13890099/68‑1 (MQ=255) aTCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGt < 1:20784590/68‑1 (MQ=255) aTCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGt < 1:24673404/68‑1 (MQ=255) aTCGCCTGGGCAATTTGCCGCGCCTACCCAACAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGt < 1:2303552/68‑1 (MQ=255) aTCCCCTGGGCAATTTTAAGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGt < 1:17914202/68‑1 (MQ=37) cGCCTGGGCAATATGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTAc < 1:2378517/68‑1 (MQ=255) gtGGGCAATTTTCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:28045245/67‑1 (MQ=255) gtGGGCAATTTTACGAGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:10989858/67‑1 (MQ=37) cTGTGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:7516316/68‑1 (MQ=255) cTGGGCATTTTGCCGAGCGTACACACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:7894498/68‑1 (MQ=255) cTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:27203779/68‑1 (MQ=255) cTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:18947100/68‑1 (MQ=255) cTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:22424282/68‑1 (MQ=255) cTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:4134981/68‑1 (MQ=255) cTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:23311120/68‑1 (MQ=255) cTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:2959989/68‑1 (MQ=255) cTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:29215331/68‑1 (MQ=255) gggCAATTTTAAGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGt < 1:17198201/68‑1 (MQ=38) gggCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGt < 1:13557580/68‑1 (MQ=255) ggaaTTTTACGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTa < 1:15468266/65‑1 (MQ=39) gcgaTTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTa < 1:9623950/64‑1 (MQ=255) gCAATTTTGAGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTa < 1:11409999/67‑1 (MQ=38) gCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTa < 1:28894420/67‑1 (MQ=255) gCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTa < 1:16407547/67‑1 (MQ=255) gCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTa < 1:11836412/67‑1 (MQ=255) aaTTTGCCGCGCCTA‑CCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTagaag > 1:25394368/1‑68 (MQ=255) tGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAgaagaa > 1:13290/1‑67 (MQ=255) | TTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAAC > CP009273/3462372‑3462503 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |