| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,462,676 | A→T | 7.0% | A179A (GCT→GCA) | chiA ← | periplasmic endochitinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,462,676 | 0 | A | T | 7.0% | 1587.8 / 27.6 | 483 | A179A (GCT→GCA) | chiA | periplasmic endochitinase |
| Reads supporting (aligned to +/- strand): ref base A (240/199); new base T (2/41); total (242/241) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.75e-11 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 488 total aligned reads displayed.
CCGGTGAAGGCTGTGGTGCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAGTTAGA > CP009273/3462609‑3462741 | gAAGGCTGTGGTGCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCgg < 1:5441825/67‑1 (MQ=255) gAAGGCTGTGGTGCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGc < 1:8677100/68‑1 (MQ=255) aGGCTGTGGTGCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCa < 1:3914420/67‑1 (MQ=255) aGGCTGTGGTGCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCa < 1:8299166/67‑1 (MQ=255) gCTGTGGTGCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGa > 1:27261603/1‑68 (MQ=255) gCTGTGGTGCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATATCCGTTGCCGTAGCGGCACGa > 1:4433071/1‑68 (MQ=255) cTGTGGTGCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGa > 1:20306998/1‑67 (MQ=255) cTGTGGAGCTGAAGTACAGTACTGTGGAATACTGGATACTGAGATAACCGTTGCCGTAGCGGCACGa > 1:10045155/1‑67 (MQ=255) tgtgGTGCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAAc < 1:773603/68‑1 (MQ=255) gtggtgCTCAAGAACAGGACTGTGGCTTACTGGTCTCTGAGATTTCCGTTGCCGTAGCGGCACGAACa < 1:18508511/68‑1 (MQ=255) gtggggCTGACGAACAGGACTGTGGATTACTGGTCTCTGAGATTTCCGTTGCCGTAGCGGCACGAACa < 1:22057084/68‑1 (MQ=255) ggtgCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATa > 1:22601655/1‑68 (MQ=255) tgCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAAc > 1:28741583/1‑68 (MQ=255) gCTGAAGTACAGGACTGTGGATTTCTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACg < 1:25179689/68‑1 (MQ=255) tGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGcc > 1:15272277/1‑68 (MQ=255) tGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGc < 1:29772438/67‑1 (MQ=255) aGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATg > 1:21820431/1‑68 (MQ=255) aGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATg > 1:7569383/1‑68 (MQ=255) aGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATg > 1:10492756/1‑68 (MQ=255) gaaCAGGACGGTGGATTACTGGATTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATgg < 1:19985270/66‑1 (MQ=37) tACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATggg > 1:18915626/1‑68 (MQ=255) tACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATggg > 1:4184143/1‑68 (MQ=255) tACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATggg > 1:16530485/1‑68 (MQ=255) aCAGGACTGTGGATTAGTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGt < 1:27914316/68‑1 (MQ=255) aCAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATgg < 1:4716121/66‑1 (MQ=255) aCAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGt > 1:8895045/1‑68 (MQ=255) aGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGtt > 1:9866524/1‑67 (MQ=255) aGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGtt > 1:22217906/1‑67 (MQ=255) aGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGtt > 1:23207090/1‑67 (MQ=255) aGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGtt > 1:15625031/1‑67 (MQ=255) ggACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGtt > 1:16799476/1‑66 (MQ=255) gACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGtt > 1:14130291/1‑65 (MQ=255) aCTGTGGATTACTGGTTTTTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:13125960/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGc < 1:1095622/66‑1 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:9599474/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:24545608/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:24510792/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:9299339/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:2278253/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:15101457/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:12691750/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:147833/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:21250420/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:13587980/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:17273733/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:16976226/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:1808847/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:16195938/1‑68 (MQ=255) cTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGc < 1:5831502/55‑1 (MQ=255) tgtgGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGc > 1:28535122/1‑68 (MQ=255) gtgGATTACTGGTTTCTGTGATTTGCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGca < 1:28083745/68‑1 (MQ=255) ggATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGcatc > 1:3244389/1‑68 (MQ=255) ggATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGcatc < 1:15925906/68‑1 (MQ=255) ggATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGcatc > 1:13345261/1‑68 (MQ=255) ggATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGcatc > 1:12425322/1‑68 (MQ=255) aTTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGcatcat > 1:27776463/1‑68 (MQ=255) aTTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGcatcat > 1:5246072/1‑68 (MQ=255) ttACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCAtt > 1:24100919/1‑68 (MQ=255) aCTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCAtt > 1:24275372/1‑66 (MQ=255) aCTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCAtt > 1:27608233/1‑66 (MQ=255) aCTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCAtt > 1:17712061/1‑66 (MQ=255) aCTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTCGCATCAtt > 1:12005340/1‑66 (MQ=255) tGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCtt > 1:25678606/1‑68 (MQ=255) tttCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTc > 1:17448143/1‑68 (MQ=255) gagaTTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATc > 1:2695046/1‑68 (MQ=255) tgaTTTCTGTTGCCGTTGCGGCTCGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCa < 1:11484762/67‑1 (MQ=17) tgaTTTCTGTTGCCGTAGCGGCTCGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCa < 1:29459152/67‑1 (MQ=18) gaTTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCa < 1:3789575/67‑1 (MQ=255) gaTTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCa < 1:11748716/67‑1 (MQ=255) gaTTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCa > 1:13816874/1‑67 (MQ=255) gaTTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCa < 1:19508701/67‑1 (MQ=255) tttCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCAcc < 1:3529924/67‑1 (MQ=255) ttCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTGCTGGCATCATTGCGTTTCGCATCACCCg > 1:29368141/1‑68 (MQ=14) ttCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCg > 1:7906243/1‑68 (MQ=255) ccgttgccgttgCGGCACGAAAATAAGGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCTGa < 1:6761767/68‑1 (MQ=255) ccGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCgg > 1:16341912/1‑67 (MQ=255) gttgccgttgccGCGCGAACATAACGCCATGGGTTTCTGGCATCATTGATTTTCGCATCACCCGGACa > 1:25963579/1‑68 (MQ=14) ttccGTAGCGGCACGAACATAACGCCATGGGTTGATGTCATCATTGCTTTTCGCATCACCCGGACAg < 1:6320539/65‑1 (MQ=38) tGCCGTTGCGGCTCGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAGt < 1:7031873/68‑1 (MQ=255) tGCCGTTGCGGCTCGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTCCGCATCACCCGGACAg < 1:24247348/67‑1 (MQ=25) tGCCGTTGCCGCGCGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAGt < 1:26448239/68‑1 (MQ=18) tGCCGTTGCCGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAg < 1:27438444/67‑1 (MQ=255) tGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAg < 1:3096688/67‑1 (MQ=255) tGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAg < 1:10172549/67‑1 (MQ=255) tGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAg < 1:13995469/67‑1 (MQ=255) tGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAg < 1:20854147/67‑1 (MQ=255) tGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAg < 1:23775918/67‑1 (MQ=255) tGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAg < 1:26686834/67‑1 (MQ=255) tGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAg < 1:3586548/67‑1 (MQ=255) tGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAGt < 1:742538/68‑1 (MQ=255) tGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAGt < 1:14311692/68‑1 (MQ=255) tGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAGt < 1:29192613/68‑1 (MQ=255) tGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAGt < 1:12209764/68‑1 (MQ=255) tGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTCTCGCATCACCCGGACAg < 1:20559587/67‑1 (MQ=255) tGCCGTAGCCGCTCGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAg < 1:5020465/67‑1 (MQ=255) gCCGTTGCCGCTCGAACATAACGACATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAGtt < 1:26263852/68‑1 (MQ=21) gCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCGCCCGGACAGtt < 1:18329457/68‑1 (MQ=255) gCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAGtt < 1:1867352/68‑1 (MQ=255) gCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAGtt < 1:11364682/68‑1 (MQ=255) cGTAGCCGCTCGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAGTTag < 1:25450243/68‑1 (MQ=255) | CCGGTGAAGGCTGTGGTGCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAGTTAGA > CP009273/3462609‑3462741 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |