BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	691,923	A→T	6.3%	noncoding (38/75 nt)	glnX ←	tRNA‑Gln

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	691,923	0	A	T	6.3%	597.9 / 17.8	180	noncoding (38/75 nt)	glnX	tRNA‑Gln
Reads supporting (aligned to +/- strand): ref base A (32/136); new base T (2/10); total (34/146)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 185 total aligned reads displayed.

AAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCT  >  CP009273/691856‑691988

aaaaGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGa                                                                   <  1:27225732/68‑1 (MQ=255)

aaaaGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGa                                                                   <  1:29389380/68‑1 (MQ=255)

  aaGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAAt                                                                 <  1:24132222/68‑1 (MQ=255)

  aaGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAAt                                                                 <  1:11349658/68‑1 (MQ=255)

  aaGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAAt                                                                 <  1:14798542/68‑1 (MQ=255)

  aaGCTCCCCGAACCGATCTTTTATAATTTGGCTGGGGTACGAGGATTCGAACCAGGGAATGCCGGTAt                                                                 <  1:14447450/68‑1 (MQ=17)

   aGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATc                                                                >  1:22596223/1‑68 (MQ=255)

   aGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATc                                                                >  1:4194573/1‑68 (MQ=255)

   aGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATc                                                                <  1:5180466/68‑1 (MQ=255)

    gCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCa                                                               >  1:7051562/1‑68 (MQ=255)

    gCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCa                                                               >  1:15141143/1‑68 (MQ=255)

    gCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCa                                                               >  1:17337058/1‑68 (MQ=255)

      tCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAg                                                              <  1:23152157/67‑1 (MQ=255)

      tCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAg                                                              <  1:20900484/67‑1 (MQ=255)

      tCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAg                                                              <  1:11470569/67‑1 (MQ=255)

      tCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAg                                                              <  1:10458014/67‑1 (MQ=255)

      tCGCCGAAGCGAGCGTTTTTAATGTGGCTGGGGTACGTGGATTCGAACCAGGGAATGCCGGTAt                                                                 <  1:13119083/64‑1 (MQ=17)

        tccGAACCGAGCTTTATTAATTTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAAt                                                           <  1:10601513/67‑1 (MQ=17)

           gAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCg                                                        >  1:5396074/1‑68 (MQ=255)

             aGCGATCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGt                                                      <  1:7275789/68‑1 (MQ=14)

             aGCGATCTTTTATAATATGGCTGGGGTACCAGGATTCGAACCTGGGAATGCCGGTATCAAAATCCGGt                                                      <  1:14045743/68‑1 (MQ=11)

             aGCGAGCTTTTTTAATTTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGt                                                      <  1:27936345/68‑1 (MQ=255)

             aGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGt                                                      <  1:17530992/68‑1 (MQ=255)

             aGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGt                                                      <  1:17491940/68‑1 (MQ=255)

             aGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGt                                                      <  1:9367856/68‑1 (MQ=255)

             aGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGt                                                      <  1:12909192/68‑1 (MQ=255)

                            tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATTCGGTTATAAGAATCAGGTGCCTTACGGGTTGGc                                       >  1:20324893/1‑68 (MQ=11)

                                         cGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTg                          <  1:2242385/68‑1 (MQ=33)

                                         cGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTg                          <  1:14728681/68‑1 (MQ=33)

                                         cGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTg                          <  1:18230976/68‑1 (MQ=33)

                                          gAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTgg                         >  1:28074078/1‑68 (MQ=35)

                                          gAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTgg                         <  1:1845453/68‑1 (MQ=35)

                                           aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATGCCCCAACtatt                        >  1:21503613/1‑65 (MQ=255)

                                           aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCaataa                          >  1:12627333/1‑63 (MQ=1)

                                           aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAAGTggg                        >  1:17723446/1‑68 (MQ=18)

                                           aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACtacg                        >  1:7087802/1‑65 (MQ=14)

                                           aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACtaat                        >  1:27338024/1‑65 (MQ=1)

                                           aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACtaat                        >  1:21502034/1‑65 (MQ=1)

                                           aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTgtg                        >  1:11937017/1‑66 (MQ=18)

                                            ggATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTggg                        <  1:12059554/67‑1 (MQ=37)

                                            ggATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGt                       >  1:22887850/1‑68 (MQ=39)

                                             gATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAGGGTg                      >  1:24807081/1‑68 (MQ=18)

                                              aTTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGt                       <  1:2878103/66‑1 (MQ=39)

                                              aTTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTg                      <  1:21685729/67‑1 (MQ=40)

                                              aTTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTg                      <  1:15224075/67‑1 (MQ=40)

                                               ttCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGc                     <  1:1713425/67‑1 (MQ=255)

                                               ttCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCa                    <  1:7788515/68‑1 (MQ=255)

                                               ttCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCa                    <  1:6057132/68‑1 (MQ=255)

                                               ttCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCa                    <  1:15094740/68‑1 (MQ=255)

                                                 cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                  <  1:6098998/68‑1 (MQ=255)

                                                 cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                  <  1:9630483/68‑1 (MQ=255)

                                                 cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                  <  1:10211495/68‑1 (MQ=255)

                                                 cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                  <  1:26312097/68‑1 (MQ=255)

                                                 cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                  <  1:9201616/68‑1 (MQ=255)

                                                 cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                  <  1:738420/68‑1 (MQ=255)

                                                 cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                  <  1:3181282/68‑1 (MQ=255)

                                                 cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                  <  1:5667269/68‑1 (MQ=255)

                                                 cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                  <  1:5205169/68‑1 (MQ=255)

                                                 cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                  <  1:4275575/68‑1 (MQ=255)

                                                 cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                  <  1:347221/68‑1 (MQ=255)

                                                 cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                  <  1:29622896/68‑1 (MQ=255)

                                                 cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                  <  1:10863768/68‑1 (MQ=255)

                                                 cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                  <  1:17973646/68‑1 (MQ=255)

                                                 cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                  <  1:18040289/68‑1 (MQ=255)

                                                 cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                  <  1:18864865/68‑1 (MQ=255)

                                                 cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                  <  1:19120972/68‑1 (MQ=255)

                                                 cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                  <  1:19457698/68‑1 (MQ=255)

                                                 cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                  <  1:24576849/68‑1 (MQ=255)

                                                 cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                  <  1:1994161/68‑1 (MQ=255)

                                                 cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                  <  1:17317453/68‑1 (MQ=255)

                                                 cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                  <  1:20512935/68‑1 (MQ=255)

                                                 cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                  <  1:21891598/68‑1 (MQ=255)

                                                 cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                  <  1:2230378/68‑1 (MQ=255)

                                                 cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                  <  1:22514800/68‑1 (MQ=255)

                                                 cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                  <  1:22713201/68‑1 (MQ=255)

                                                 cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                  <  1:13008607/68‑1 (MQ=255)

                                                 cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                  <  1:2334191/68‑1 (MQ=255)

                                                 cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                  <  1:15672575/68‑1 (MQ=255)

                                                 cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                  <  1:15216396/68‑1 (MQ=255)

                                                    aCCTCGGAATTCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTa                <  1:22204514/67‑1 (MQ=255)

                                                    aCCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTa                <  1:15916520/67‑1 (MQ=255)

                                                    aCCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTa                <  1:27956456/67‑1 (MQ=255)

                                                    aCCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTa                <  1:21102890/67‑1 (MQ=255)

                                                    aCCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTa                <  1:7961109/67‑1 (MQ=255)

                                                    aCCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTa                <  1:768020/67‑1 (MQ=255)

                                                       tCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAg            <  1:14665213/68‑1 (MQ=255)

                                                       tCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAg            <  1:20441972/68‑1 (MQ=255)

                                                       tCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAg            <  1:13317553/68‑1 (MQ=255)

                                                       tCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAg            <  1:25976232/68‑1 (MQ=255)

                                                       tCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAg            <  1:25041427/68‑1 (MQ=255)

                                                        cGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAgg           <  1:28519346/68‑1 (MQ=255)

                                                          gAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCAATTACAAgtga         >  1:25171205/1‑65 (MQ=255)

                                                           aaTGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTa         <  1:6574630/67‑1 (MQ=255)

                                                            aTGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTaa        >  1:17583975/1‑67 (MQ=255)

                                                                cGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGt    <  1:19888696/67‑1 (MQ=255)

                                                                cGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGt    <  1:16924953/67‑1 (MQ=255)

                                                                cGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGt    <  1:29419937/67‑1 (MQ=255)

                                                                cGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGt    <  1:26965040/67‑1 (MQ=255)

                                                                cGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGt    <  1:24328507/67‑1 (MQ=255)

                                                                   aaTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAg            <  1:8047705/56‑1 (MQ=255)

AAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCT  >  CP009273/691856‑691988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`