| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 999,590 | A→C | 5.1% | N63T (AAC→ACC) | ycbF → | putative periplasmic pilin chaperone |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 999,590 | 0 | A | C | 5.1% | 2206.9 / 10.0 | 700 | N63T (AAC→ACC) | ycbF | putative periplasmic pilin chaperone |
| Reads supporting (aligned to +/- strand): ref base A (485/169); new base C (3/43); total (488/212) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.21e-20 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 717 total aligned reads displayed.
AGAATCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCA > CP009273/999525‑999657 | gaATCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAAc < 1:24813696/66‑1 (MQ=255) aTCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGcc < 1:19525615/68‑1 (MQ=255) aTCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGc > 1:25047097/1‑67 (MQ=255) cGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCa < 1:20952311/68‑1 (MQ=255) atCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAAcgc < 1:18651624/68‑1 (MQ=255) atCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAAcgc < 1:8753352/68‑1 (MQ=255) tCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAAcgcg > 1:36954242/1‑68 (MQ=255) ttttACTGACTAATACCAGTCAGGAATCCTGGCTTATTAATAGTAAAATCAACCGCCCAACGCGTTgg > 1:14056326/1‑68 (MQ=255) ttttACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTgg > 1:23668952/1‑68 (MQ=255) ttttACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTgg > 1:21775960/1‑68 (MQ=255) tttACTGACTAATACCACTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTgg > 1:26344652/1‑67 (MQ=255) ttACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTggg > 1:10840673/1‑67 (MQ=255) aaCTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCACAAAGCGTTGTGcc > 1:12275794/2‑68 (MQ=255) aCTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCg > 1:10000184/1‑68 (MQ=255) aCTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCg > 1:36173320/1‑68 (MQ=255) aCTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCg > 1:15094211/1‑68 (MQ=255) aCTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCg > 1:28252129/1‑68 (MQ=255) aCTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGAGTTGGGCCg > 1:35554447/1‑68 (MQ=255) tGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCggg > 1:38214025/1‑68 (MQ=255) tGACTAATACCAGTCAGGAATCCTGGCTTATTAACACTAAAATCAACCGCCCAACGCGTAGGGCCggg > 1:35316564/1‑68 (MQ=255) tGACTAATACCAGGCAGGAATCCTGGCTTATAAACAGTAAAACCAACCGCCCAACGCGTTGGGCCggg < 1:306730/68‑1 (MQ=255) cTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCgggggg > 1:15350921/1‑68 (MQ=255) tACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAg < 1:13884748/68‑1 (MQ=255) tACCAGTCAGGAATCCTGGCTTATAAACAGTAAAATCAACCCCCCAACGCGTTGGGCCGGGGGGGAAg < 1:16025051/68‑1 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAACTCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:7070354/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCACCGCGTTGGGCCGGGGGGGAAGc > 1:28816240/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:25919242/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:29442562/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:11147838/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:25365760/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:30985679/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:31350065/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:38623180/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:32638945/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:32936006/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:37836107/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:34817661/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:35839226/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:10221443/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:2037084/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:13158119/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:23258004/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:15668161/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:16735808/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:23847637/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:20541830/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:8078471/1‑68 (MQ=255) ccAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCg > 1:9035172/1‑68 (MQ=255) cAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCg > 1:6368830/1‑67 (MQ=255) cAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGAGTTGGGCCGGGGGGGAAGCg > 1:19110461/1‑67 (MQ=255) tCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCg > 1:29035512/1‑67 (MQ=255) tCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCg > 1:1994374/1‑67 (MQ=255) ggAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAg > 1:6536362/1‑68 (MQ=255) gAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGt > 1:33900675/1‑68 (MQ=255) aaTCCTTGATAATTAACATAAAAATCACCCGCCCATCGCGTTGGGCCGGGGGGGAAGCGTCGACAGTg < 1:5525218/68‑1 (MQ=255) aaTCCTGTATTATTAACAGTAAAATCACCCCCCCCCCGCGGGGGGCCGGGGGGGAAGCGTCGACAGt < 1:11457794/67‑1 (MQ=255) aaTCCTGGCTTATTAACAGTAAAATCAACCGCCCACCGCGTTGGGCCGGGGGGGAAGCGTCGACAGTg < 1:22102429/68‑1 (MQ=255) aaTCCTGGCTTATTAACAGTAAAATCAACCCCCCACCGCGTGGGGCCGGGGGGGAAGCGTCGACAGTg < 1:27575852/68‑1 (MQ=255) aTCGTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTg < 1:11868099/67‑1 (MQ=255) aTCCTGGCTTATTAACAGTAAAATCACCCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTg < 1:4735495/67‑1 (MQ=255) aTCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTg > 1:29916105/1‑67 (MQ=255) aTCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTg > 1:3983338/1‑67 (MQ=255) aTCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTg > 1:6023757/1‑67 (MQ=255) aTCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTg < 1:18420605/67‑1 (MQ=255) aTCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTg > 1:8381123/1‑67 (MQ=255) aTCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTg > 1:17410538/1‑67 (MQ=255) aTCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTg < 1:27051375/67‑1 (MQ=255) aTCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTg < 1:33503487/67‑1 (MQ=255) aTCCTGGCTTATAAACAGAAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTg < 1:24293508/67‑1 (MQ=255) ccTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCa > 1:5786924/1‑68 (MQ=255) ccTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCa > 1:29700045/1‑68 (MQ=255) tGGCTTATTAACATTAAAAACAACCCCCCACCGCGGGGGGCCGGGGGGGAAGCGTCGACAGTGCCAGc < 1:14381436/68‑1 (MQ=255) ttcTTATTAACTGTAAAATCAACCGCCCACCGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCa < 1:2863067/66‑1 (MQ=255) gtcTTATAAACATTAAAATCACCCGCCCCCCGCGGTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCa < 1:17849229/66‑1 (MQ=255) ggCTTATTAACAGTAAAATCACCCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCa < 1:33350675/68‑1 (MQ=255) ggCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCa < 1:26817228/68‑1 (MQ=255) gCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCa > 1:9517784/1‑67 (MQ=255) gCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCa > 1:14725306/1‑67 (MQ=255) gCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCa > 1:25555630/1‑67 (MQ=255) ctaattaACAGTAAATTCACCGCCCCCCCGCGTGGGGCCGGGGGGGAAGAGGCGACAGTGCCAGCAcc < 1:31671100/65‑1 (MQ=255) cTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCAcc > 1:27786048/1‑68 (MQ=255) ttataaACAGTAAAACCACCCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCAcc < 1:31987071/67‑1 (MQ=255) attaACAGTAAAATCACCCGCCCACCGGGTGGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCAtt < 1:5020551/68‑1 (MQ=255) taACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCAtt > 1:37523261/1‑66 (MQ=255) taACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCAtt > 1:13418176/1‑66 (MQ=255) taACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCAtt > 1:21410379/1‑66 (MQ=255) aaCAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTTCCAGCACCAtt > 1:18046909/1‑65 (MQ=255) aCAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTg > 1:22753569/1‑68 (MQ=255) aCAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTg > 1:17071723/1‑68 (MQ=255) aCAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTg > 1:36524538/1‑68 (MQ=255) aaaaTCAACCGCCCACCGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTgg < 1:7485602/64‑1 (MQ=255) aaaTCACCCGCCCACCCCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCt < 1:7823166/68‑1 (MQ=255) aaaTCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCt < 1:32389933/68‑1 (MQ=255) aTCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTcc > 1:18817594/1‑68 (MQ=255) aTCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTcc > 1:6751737/1‑68 (MQ=255) cAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCg > 1:16266261/1‑67 (MQ=255) cAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCg > 1:34516514/1‑67 (MQ=255) aaCCGCCCACCGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGcc < 1:4407691/68‑1 (MQ=255) aCCGCCCACCGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCa < 1:12829913/68‑1 (MQ=255) aCCGCCCAACGCGTGGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCa < 1:30118524/68‑1 (MQ=255) | AGAATCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCA > CP009273/999525‑999657 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |