| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 298,191 | T→G | 10.1% | S32R (AGC→CGC) | paoA ← | PaoABC aldehyde oxidoreductase, 2Fe‑2S subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 298,191 | 0 | T | G | 10.1% | 1348.1 / 22.0 | 465 | S32R (AGC→CGC) | paoA | PaoABC aldehyde oxidoreductase, 2Fe‑2S subunit |
| Reads supporting (aligned to +/- strand): ref base T (73/337); new base G (52/2); total (126/339) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.76e-31 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 495 total aligned reads displayed.
CTGCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCGGCTCGTGCTTCCCAACCCGATTATCT > CP009273/298125‑298258 | cTGCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCGGGTGCGGCGCTc > 1:27588311/1‑68 (MQ=255) tGCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCa < 1:36343001/68‑1 (MQ=255) tGCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCa < 1:37929585/68‑1 (MQ=255) tGCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGGTGCGGCGCTCa < 1:6703982/68‑1 (MQ=255) gCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCAc < 1:29973073/68‑1 (MQ=255) gCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCAc < 1:5903380/68‑1 (MQ=255) gCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCAc < 1:15518235/68‑1 (MQ=255) gCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCAc < 1:22190677/68‑1 (MQ=255) gCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCAc < 1:31940908/68‑1 (MQ=255) gCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCAc < 1:24295581/68‑1 (MQ=255) gCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCAc < 1:12783369/68‑1 (MQ=255) gCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCAc < 1:38311425/68‑1 (MQ=255) gCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCAc < 1:11884426/68‑1 (MQ=255) gCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCAc < 1:11374611/68‑1 (MQ=255) gCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCAc < 1:26320098/68‑1 (MQ=255) gCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGGCGCCGCTGTTGCGGCGCTCAc < 1:32867323/68‑1 (MQ=255) gCCGGAACGCTTGCAGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCAc < 1:27069096/68‑1 (MQ=255) cGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACtt < 1:30999785/68‑1 (MQ=255) cGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACtt < 1:5541312/68‑1 (MQ=255) cGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACt < 1:8236095/67‑1 (MQ=255) ggAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACttt < 1:15102381/68‑1 (MQ=255) ttGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTGGCCGCTGTTGCGGCGCTCACTTTAATCAGa < 1:37189253/68‑1 (MQ=255) ttGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGa < 1:22819442/68‑1 (MQ=255) ttGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGa < 1:18226512/68‑1 (MQ=255) ttGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGa < 1:34966891/68‑1 (MQ=255) ttGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGa < 1:7209474/68‑1 (MQ=255) gTAGAATGAGTATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTATTCAGATGGCGACggg < 1:4885565/67‑1 (MQ=255) gTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACggg < 1:13498029/67‑1 (MQ=255) gTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACggg < 1:20480763/67‑1 (MQ=255) gTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACgg < 1:6419131/66‑1 (MQ=255) aGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACATTAATCAGAACGCGACGGGtga > 1:26033791/1‑66 (MQ=255) aaTGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCaaa < 1:10788129/68‑1 (MQ=255) aaTGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCaaa < 1:31699327/68‑1 (MQ=255) aTGAGGATAAACCACGGCGGTCGCCGCTGTTTGGGCGCGCACATTAATCAGCTCGACACCGGTCaaaa > 1:16413638/1‑67 (MQ=255) aTGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCaaa < 1:7620038/67‑1 (MQ=255) aTGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCaaa < 1:37464809/67‑1 (MQ=255) aTGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCaaa < 1:25712043/67‑1 (MQ=255) aTGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCaaa < 1:12306864/67‑1 (MQ=255) aTGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCaaa < 1:38703551/67‑1 (MQ=255) aTGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCaaa < 1:7897864/67‑1 (MQ=255) aTGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCaaa < 1:14136437/67‑1 (MQ=255) aTGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCaaa < 1:133681/67‑1 (MQ=255) aTGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCaaa < 1:24498875/67‑1 (MQ=255) aTGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCaaa < 1:22565082/67‑1 (MQ=255) aTGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCaaa < 1:14732934/67‑1 (MQ=255) aTGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCaaa < 1:21210305/67‑1 (MQ=255) aTGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCaaa < 1:35537743/67‑1 (MQ=255) aTGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCaaa < 1:34494907/67‑1 (MQ=255) aTGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCaaa < 1:17962396/67‑1 (MQ=255) aTGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCaaa < 1:30245813/67‑1 (MQ=255) aTGAGGATAAACCACGGCGGTCGCCGCGGTTGCGGCGCTCACTTTAATAAGATAGCGCCGGGTCAAAc > 1:18593906/1‑68 (MQ=255) aTGAGGATAAACCACGGCGGTCGCCGCGGTTGCGGCGCGCACATTAATAAGATCGCGCCGGGTGaa > 1:30718620/1‑66 (MQ=255) tGAGGATAAACCACGGCGGGCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAAACt < 1:27762509/68‑1 (MQ=255) gAGGATAAACCACGGCGGTCGCCGCGGTTGCGGCGCGCACTTTAATAAGCTCGCGCCCGGGCaaaaat > 1:17382765/1‑65 (MQ=255) gATAAACCACGGCGGTCGCCGCGGTTGCGGGGCGCACATTAATCAGATGGCGCCGGGGGAAACATaaa > 1:17612029/1‑68 (MQ=255) gATAAACCACGGCGGTCGCCGCGGTTGCGGCGCTCACTTTAAt > 1:35935855/1‑43 (MQ=255) gATAAACCACGGCGGTCGCCGCGGTTGCGGCGCTCAAGTTAATCAGCTGGCGGCGGGTGAAACGTaaa > 1:21902372/1‑68 (MQ=255) gATAAACCACGGCGGTCGCCCCGGTTGCGGCGCTCACTTTAAAAAGAAAGCGGCGGGGCAAAATaaaa > 1:29016397/1‑68 (MQ=255) gATAAACCACGGCGGTCCCCGCGGTTGACGCGCTGACTTTAAAAATAAAGCCACGGGTGAAACTTaaa > 1:24803558/1‑68 (MQ=255) gATAAACCACGGCGGTCCCCGCGGGTGCGGCCCGCACATTAATGAGATCGCGCCGGGGCCAACTTtaa > 1:29220772/1‑68 (MQ=255) aaaCCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCg < 1:5119478/68‑1 (MQ=255) aaCCACGGCGGTCGCCGCTGTTGGGGCGCGCACGTTTATCAGCTAGCGACGGGTCAAACTTAAAACGt > 1:28627032/1‑68 (MQ=255) aaCCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGt < 1:16659453/68‑1 (MQ=255) aaCCACGGCGGTCGCCGCGGTTGCGGGGCGCACGTTTATCAGATAGCGACGGGTGAAACTTAAATAGt > 1:3734975/1‑68 (MQ=255) aCCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATAAGATAGCGCCGGGGCAAACTTAAATAGt > 1:5324592/1‑67 (MQ=255) ccACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATAGCGACGGGTCAAACTTAAATCGTGc > 1:38933967/1‑68 (MQ=255) aCGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCgg < 1:36941723/68‑1 (MQ=255) aCGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCgg < 1:33108228/68‑1 (MQ=255) aCGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCgg < 1:4051379/68‑1 (MQ=255) aCGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCgg < 1:28095800/68‑1 (MQ=255) cggcggTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCGGc < 1:23193934/68‑1 (MQ=255) cggcggTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCGGc < 1:15989461/68‑1 (MQ=255) ggTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCGGCTCGt < 1:32426911/68‑1 (MQ=255) cgccgcTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCGGCTCGTGCt < 1:33753357/68‑1 (MQ=255) gccgcTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCGGCTCGTGCtt < 1:33532268/68‑1 (MQ=255) cgcTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCGGCTCGTGCTTc < 1:10424114/67‑1 (MQ=255) cgcTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCGGCTCGTGCTTc < 1:3140193/67‑1 (MQ=255) cgcTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCGGCTCGTGCTTc < 1:26914298/67‑1 (MQ=255) cgcTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCGGCTCGTGCTTc < 1:2617516/67‑1 (MQ=255) cgcTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCGGCTCGTGCTTc < 1:23627098/67‑1 (MQ=255) gcTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCGGCTCGTGCTTccc > 1:12039186/1‑68 (MQ=255) tGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCGGCTCGTGCTTCCCAcccc > 1:19681771/1‑68 (MQ=255) tGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCGGCTCGTGCTTCCCAAccc > 1:9119251/1‑68 (MQ=255) tGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCGGCTCGTGCTTCCCAAccc > 1:10534795/1‑68 (MQ=255) tGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCGGCTCGTGCTTCCCAAccc > 1:19118349/1‑68 (MQ=255) tGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCGGCTCGTGCTTCCCAAcc < 1:25097427/67‑1 (MQ=255) tGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCGGCTCGTGCTTCCCAAcc < 1:23781968/67‑1 (MQ=255) ggcgCTCACTTTAATCAGATCGCGCCGGGTCAAACTTAAATCGTGCGGCTCGTGCTTCCCAACCCGAt > 1:11056956/1‑68 (MQ=255) ggcgCTCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCGGCTCGTGCTTCCCAACCCGa < 1:17269690/67‑1 (MQ=255) ggcgCTCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCGGCTCGTGCTTCCCAACCCGAt > 1:32698577/1‑68 (MQ=255) ggcgCTCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCGGCTCGTGCTTCCCAACCCGAt < 1:20045434/68‑1 (MQ=255) ggcgCTCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCGGCTCGTGCTTCCCAACCCGAt > 1:2525846/1‑68 (MQ=255) ggcgCTCACTTTAATCAGATCGCGACGGGGCAAACTTAAATCGTGCGGCTCGTGCTTCCCAACCCGAt < 1:8772515/68‑1 (MQ=255) cgcTCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCGGCTCGTGCTTCCCAACCCGATTa > 1:26647394/1‑68 (MQ=255) cgcTCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCGGCTCGTGCTTCCCAACCCGATTa > 1:10006772/1‑68 (MQ=255) cgcTCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCGGCTCGTGCTTCCCAACCCGATTa > 1:9606225/1‑68 (MQ=255) tCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCGGCTCGTGCTTCCCAACCCGATTATc < 1:18994506/67‑1 (MQ=255) tCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCGGCTCGTGCTTCCCAACCCGATTATc < 1:16943053/67‑1 (MQ=255) tCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCGGCTCGTGCTTCCCAACCCGATTATc < 1:34189285/67‑1 (MQ=255) tCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCGGCTCGTGCTTCCCAACCCGATTATCt > 1:20746153/1‑68 (MQ=255) | CTGCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCGGCTCGTGCTTCCCAACCCGATTATCT > CP009273/298125‑298258 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |