| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,462,439 | C→A | 8.7% | A258A (GCG→GCT) ‡ | chiA ← | periplasmic endochitinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,462,439 | 0 | C | A | 8.7% | 2171.6 / 56.1 | 724 | A258A (GCG→GCT) ‡ | chiA | periplasmic endochitinase |
| Reads supporting (aligned to +/- strand): ref base C (146/503); new base A (44/31); total (190/534) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.29e-10 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 767 total aligned reads displayed.
GCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTT > CP009273/3462374‑3462506 | cgcgcgAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCcgcg < 1:37217879/67‑1 (MQ=255) gcgcgAACATCACGCCCTGGGGTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGcc < 1:36530582/68‑1 (MQ=255) gcgcgAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGcc < 1:35215050/68‑1 (MQ=255) gcgcgAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGcc < 1:12620893/68‑1 (MQ=255) aCATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACcca < 1:25840486/68‑1 (MQ=255) aCATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACcca < 1:18608350/68‑1 (MQ=255) cATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACcca > 1:29173030/1‑67 (MQ=255) aTAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACccacc > 1:14047147/1‑68 (MQ=255) aCGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGAAGAGGATACCCACCAg > 1:5874705/1‑67 (MQ=18) cGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGTAGATGATACCCACCAGGc > 1:16472263/1‑68 (MQ=18) cGCCATGGGTTACTGGCATCGTTTTCTTTCGCAACGCCTGGGAAATTTGCCGCGCCTACCCACCAGGc > 1:21686549/1‑68 (MQ=255) gCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCATTTTGAAGCGCCTACCCACCAGGca > 1:8392091/1‑67 (MQ=25) gCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCg > 1:10845072/1‑68 (MQ=255) gCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCg > 1:11589575/1‑68 (MQ=255) gCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCAGGGCAATTAGCAGAGGCTACCCACCAGGca > 1:16234289/1‑67 (MQ=17) ccATGGGTTACTGGCATCGTTTTGTTTCGCATCGCCCGGTCATTTAGAAGAGCCTACCCACCAGGcat > 1:38743089/1‑66 (MQ=2) ccATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGt > 1:14673639/1‑68 (MQ=255) ccATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGt > 1:11075275/1‑68 (MQ=255) ccATGGGTTACTGGCATCGTTTTCTTTCGCATATCCTGGGCAATTTGAAGAGGCTACCCACCAGGcat > 1:18444040/1‑66 (MQ=17) cATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGtt < 1:2273586/68‑1 (MQ=255) cATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGtt < 1:13539071/68‑1 (MQ=255) cATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGt < 1:34370603/67‑1 (MQ=255) cATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGt < 1:25683782/67‑1 (MQ=255) cATGGGTTACTGGCATCGTTTTCTATCGCATCGCGTGGGCAATTTGACGCGCCTACCCACCAGGCGtt < 1:30597060/68‑1 (MQ=38) aTGGGTTACTGGCCTCGTATTCTATCGCATCGCGTGGGCAATTTTCCGCGCCTACCCACCAGGCGTTc < 1:9314707/68‑1 (MQ=255) aTGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTc < 1:36278392/68‑1 (MQ=255) tGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTTCCGCGCCTACCCACCAGGCGTTCt < 1:24353067/68‑1 (MQ=255) tGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCt < 1:5307691/68‑1 (MQ=255) tGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCt < 1:25143376/68‑1 (MQ=255) gggTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCtt > 1:27891782/1‑68 (MQ=255) gTTACTGGCATCGTTTGCTATCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTta < 1:21965000/67‑1 (MQ=255) atGGCCTCTTTTGCTATCGCATCGCGTGGGCAATTTTCAGCGCCTACCCACCAGGCGTTCTTATAAAt < 1:31089030/67‑1 (MQ=21) tGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAAt > 1:20115906/1‑67 (MQ=255) tGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAAt > 1:12168663/1‑67 (MQ=255) tGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGCCGTTATTATAAAt > 1:16831679/1‑67 (MQ=255) tGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGAAGCGCCTACCCACCAGGCATTATTATATATg > 1:19860347/1‑68 (MQ=21) tGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTAGCCGCGCATACACACCAGCCTTTCTTATAAAt > 1:31755053/1‑67 (MQ=255) ggCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATg > 1:7836242/1‑67 (MQ=255) ggCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATg > 1:11286761/1‑67 (MQ=255) ggCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATg > 1:28578243/1‑67 (MQ=255) tCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATgccgcc < 1:35720037/68‑1 (MQ=255) tCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATgccgcc < 1:2094337/68‑1 (MQ=255) tCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATgccgcc > 1:17839293/1‑68 (MQ=255) tCGTTTTCTATCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATgccgcc < 1:34843438/68‑1 (MQ=255) ttttgCTTTCGCATCGCCTGGGCAATTTTCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:28809289/67‑1 (MQ=39) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:3342622/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:8094326/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:37630680/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:26463731/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:7124508/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:394781/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:8674/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:4662741/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:21421182/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:9646193/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:13207227/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:18088175/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:19036365/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:22245084/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:24078595/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:2476500/68‑1 (MQ=255) cggttctttcGCATCGCCTGGGCAATTTGGCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:20439212/65‑1 (MQ=255) ttttCTATGGCACCGCGTGGGCAATATTCCGAGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGtt < 1:4975885/68‑1 (MQ=21) ttctttcGCATCCCCTGGGCAATTTGACGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTtat < 1:7331419/68‑1 (MQ=255) ctttgGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAg < 1:28118509/68‑1 (MQ=255) ctttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAg < 1:6568916/68‑1 (MQ=255) tttcGCATCGCCTGGGCAATTTGCCGCGCCT‑CCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:23177060/68‑1 (MQ=255) ttggCATCGCCTGGGCAATTTGACGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:27478793/65‑1 (MQ=255) ttcGGATCGCGTGGGCAATTTGCAGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:19575247/68‑1 (MQ=38) ttcGCATCGCCTGGGCAATTTTCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:314785/68‑1 (MQ=255) ttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:2831596/68‑1 (MQ=255) ttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:13813835/68‑1 (MQ=255) ttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:15920271/68‑1 (MQ=255) ttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:26684030/68‑1 (MQ=255) ttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:8896830/68‑1 (MQ=255) ttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:29598230/68‑1 (MQ=255) cGCATCGCCTGGGCAATATGACGAGCCAACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATaa < 1:32533619/68‑1 (MQ=37) aTCGCCTGGTCAATTTGGCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGt < 1:10046675/68‑1 (MQ=255) aTCGCCTGGGCAATTTTTCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGt < 1:15455865/68‑1 (MQ=255) aTCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGt < 1:20693277/68‑1 (MQ=255) cTGGGCAATTTTTCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:21155737/68‑1 (MQ=255) cTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:32880106/68‑1 (MQ=255) cTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:33047124/68‑1 (MQ=255) cTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:38383557/68‑1 (MQ=255) cTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:33967545/68‑1 (MQ=255) atGGTCATTTTTCCGAGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:29887402/67‑1 (MQ=38) gCAATTTTCCGAGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTa < 1:14438397/67‑1 (MQ=255) gCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTag > 1:4380312/1‑68 (MQ=255) gCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTag > 1:26159610/1‑68 (MQ=255) gCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTa < 1:23491859/67‑1 (MQ=255) cAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTag < 1:12850436/67‑1 (MQ=255) cAATTTGCCGCGCCTACCAACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTag < 1:30360934/67‑1 (MQ=255) ttGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTagaaga > 1:15324129/1‑67 (MQ=255) gCCGCGCCTACCCCCCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAgaagaa > 1:6852836/1‑66 (MQ=255) gCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAgaagaa > 1:30190257/1‑66 (MQ=255) cgcgCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCtt < 1:27036818/68‑1 (MQ=255) cgcgCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCtt < 1:631703/68‑1 (MQ=255) cgcgCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCtt < 1:37926764/68‑1 (MQ=255) cgcgCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCtt < 1:17304107/68‑1 (MQ=255) cgcgCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCtt < 1:10590909/68‑1 (MQ=255) | GCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTT > CP009273/3462374‑3462506 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |