BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	692,313	G→T	5.3%	intergenic (‑27/+8)	glnW ← / ← glnU	tRNA‑Gln/tRNA‑Gln

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	692,313	0	G	T	5.3%	1146.0 / 6.8	351	intergenic (‑27/+8)	glnW/glnU	tRNA‑Gln/tRNA‑Gln
Reads supporting (aligned to +/- strand): ref base G (244/83); new base T (15/9); total (259/92)
Fisher's exact test for biased strand distribution p-value = 2.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 366 total aligned reads displayed.

CGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACC  >  CP009273/692246‑692379

 ggTATCACAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCga                                                                   <  1:35479813/68‑1 (MQ=255)

 ggTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGGTTACTCga                                                                   >  1:4390503/1‑68 (MQ=255)

    aTCACAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCgaaga                                                                <  1:13268948/68‑1 (MQ=255)

    aTCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCgaaga                                                                <  1:16576038/68‑1 (MQ=255)

         aaaCCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATg                                                           <  1:33543000/68‑1 (MQ=255)

         aaaCCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATg                                                           <  1:31909374/68‑1 (MQ=255)

           tccGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGc                                                         <  1:35766794/67‑1 (MQ=255)

           aCCGGTGCCTTACCGCTTGGCGATACCCCAATAGCCGGGCGGTGACCCGCGTAATCTGAGAAGATg                                                           >  1:2875855/1‑66 (MQ=18)

           aCCGGTGCCTTACCGCTTGGCGATACCCCAATACCCGGGCGGTGAACCGCGTAGTCGAAGCAGTTg                                                           >  1:3874998/1‑66 (MQ=21)

           aCCGGTGCCTTACCGCTTGGCGATACCCCAATACCCGGGCGGTGAAACGCTTACTCGTAGAAGATGGc                                                         >  1:26704/1‑68 (MQ=25)

           aCCGGTGCCTTACCGCTTGGCGATACCCCAATAAACGGGCGGTGTCCCGCGTACGCTGAGAAGATg                                                           >  1:37981284/1‑66 (MQ=17)

           aCCGGTGCCTTACCGCTTGGCGATACCCCAAAACGCGGGCGGTGACCCGCTTACTGGAAGAAGATg                                                           >  1:7812596/1‑66 (MQ=21)

           aCCGGTGCCTTACCGCTTGGCGATACCCCAAAACCCGGGCGCTGAACCGGTTACTCGAAGATGATGGc                                                         >  1:21690248/1‑68 (MQ=21)

            ccGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCt                                                        <  1:30468518/68‑1 (MQ=255)

            ccGGTGCCTTACCGCTTGGCGATACCCCAATAACAGGGCGGTGAAACGCTTACTCGCAGAAGATg                                                           >  1:36730791/1‑65 (MQ=25)

              ggTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTgg                                                      <  1:4262481/68‑1 (MQ=255)

              ggTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTgg                                                      <  1:21413640/68‑1 (MQ=255)

              ggTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTgg                                                      <  1:22215179/68‑1 (MQ=255)

                tGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTggg                                                     <  1:14145224/67‑1 (MQ=255)

                 gCCTTACCGCTTGGCGATACCCCAATAACCGGGGGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGt                                                   <  1:1024242/68‑1 (MQ=255)

                 gCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTgggg                                                    <  1:29466713/67‑1 (MQ=255)

                 gCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTgggg                                                    <  1:31768356/67‑1 (MQ=255)

                 gCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTgggg                                                    <  1:22364412/67‑1 (MQ=255)

                  ccTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGt                                                   <  1:10485710/67‑1 (MQ=255)

                   cTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTAc                                                 <  1:18015460/68‑1 (MQ=255)

                   cTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTAc                                                 <  1:24086282/68‑1 (MQ=255)

                   cTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTAc                                                 <  1:6159049/68‑1 (MQ=255)

                   cTTACCGCTTGGAGATACCCCAATAACCGGGCGGTGAACCGCGTACTCGAAGAAGATGGCTGGGGTAc                                                 <  1:37906522/68‑1 (MQ=39)

                   attACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTaa                                                 >  1:12363288/2‑67 (MQ=255)

                    ttACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTAcc                                                <  1:34197480/68‑1 (MQ=255)

                      aCCGCTTGGCGATACCCCAAAAAGCGGGCGGTTAACCGCTAAGTCGAAGAAGATGGCTGGGGTACCTg                                              >  1:7899358/1‑68 (MQ=37)

                        cGCTTGGCGATACCCCAATACCCGGGCGGTGACCCGCTTACTCGAAGAAGATGGCTGGGGTACCTg                                              >  1:13793748/1‑66 (MQ=255)

                        cGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTAGTCGAAGAAGATGGCTGGGGTACCTGGa                                            >  1:32925336/1‑68 (MQ=255)

                        cGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGa                                            >  1:22819298/1‑68 (MQ=255)

                        cGCTTGGCGATACCCCAAAAGCCGGGCGCTGAACCGGTTAATCTAAGATCATGGCTGGGGTACCTCGa                                            >  1:27045313/1‑68 (MQ=255)

                         gCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGAt                                           >  1:11912965/1‑68 (MQ=255)

                         gCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAACTGGCTGGGGTACCTGGAt                                           >  1:23096558/1‑68 (MQ=255)

                           ttGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTc                                         >  1:5677795/1‑68 (MQ=255)

                             ggCGATACCCCAATAACCGTGCTTTGAACCGAGTACTCGAAGTCGATGGCTGGGGTACCTGGATTCGa                                       <  1:12950972/68‑1 (MQ=21)

                             ggCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGa                                       <  1:19706281/68‑1 (MQ=255)

                             ggCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATAGCTGGGGTACCTGGATTCGa                                       <  1:36879780/68‑1 (MQ=255)

                              gCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTTCCTGGATTCGa                                       >  1:26442172/1‑67 (MQ=255)

                              gCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGa                                       >  1:37563007/1‑67 (MQ=255)

                               ctaTACCAAAATAACCGGGCTCTGAACCGCTTTCTCTAATAAGATGGCTGGGGTACCTGGATTCGaa                                      <  1:33857014/65‑1 (MQ=18)

                               cgttACCTCAATCACCGGTCGGTGAACACCTTTCTGGAATAACATGGGTGGGGTACCTGGATTCGaa                                      <  1:17043403/64‑1 (MQ=12)

                               cGATACCCCAATAACCGGGCGGTGAACCGCTTACTCTAAGAAGATGGCTGGGGTACCTGGATTCGaa                                      <  1:28368028/67‑1 (MQ=255)

                               cGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGaa                                      <  1:14790792/67‑1 (MQ=255)

                               cGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGaa                                      <  1:11056287/67‑1 (MQ=255)

                               cGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGaa                                      <  1:15108663/67‑1 (MQ=255)

                                gATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAAcc                                    >  1:38436529/1‑68 (MQ=255)

                                gATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAAcc                                    >  1:35165577/1‑68 (MQ=255)

                                gATACCCCAATAACCGCGCGGTGAACCGCGTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAAcc                                    <  1:11441445/68‑1 (MQ=255)

                                gATACCACAATAACCTCGCGGTGAACAGCTTTCTCTTATAATATGGCTGGGGTACCTGGATTCGAAcc                                    <  1:22675638/68‑1 (MQ=16)

                                 aTACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCa                                   >  1:24904720/1‑68 (MQ=255)

                                   aCCCCAATCACCCCGCGGGGAACCGCTTACTATAATAATATGGCTGGGGTACCTGGATTCGAACCAgg                                 <  1:24812999/68‑1 (MQ=18)

                                    ccccAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAggg                                >  1:21844491/1‑68 (MQ=255)

                                    ccccAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAggg                                >  1:36317169/1‑68 (MQ=255)

                                    ccccAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAggg                                >  1:27359814/1‑68 (MQ=255)

                                     cccAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGa                               >  1:38991765/1‑68 (MQ=255)

                                     cccAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGa                               >  1:30920203/1‑68 (MQ=255)

                                     cccAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGa                               >  1:28199648/1‑68 (MQ=255)

                                     cccAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGa                               >  1:17890530/1‑68 (MQ=255)

                                     cccAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGa                               >  1:26947687/1‑68 (MQ=255)

                                     cccAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGa                               >  1:24612412/1‑68 (MQ=255)

                                     cccAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGa                               >  1:9084386/1‑68 (MQ=255)

                                     cccAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGa                               >  1:23715890/1‑68 (MQ=255)

                                       cAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATgg                                                          <  1:25030934/39‑1 (MQ=255)

                                         aTAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATg                            >  1:30117288/1‑67 (MQ=255)

                                          tAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGcc                          >  1:36621200/1‑68 (MQ=255)

                                           aaCCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCg                         >  1:13418052/1‑68 (MQ=255)

                                           aaCCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCg                         >  1:7176695/1‑68 (MQ=255)

                                           aaCCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCg                         >  1:3319047/1‑68 (MQ=255)

                                           aaCCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCg                         >  1:1597323/1‑68 (MQ=255)

                                           aaCCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCg                         >  1:162216/1‑68 (MQ=255)

                                           aaCCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCg                         >  1:2555996/1‑68 (MQ=255)

                                            aCCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCgg                        >  1:867957/1‑68 (MQ=255)

                                            aCCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCgg                        >  1:16912880/1‑68 (MQ=255)

                                            aCCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCgg                        >  1:27803537/1‑68 (MQ=255)

                                            aCCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCgg                        >  1:30646489/1‑68 (MQ=255)

                                              cGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTa                      >  1:11577321/1‑68 (MQ=255)

                                                 gCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCa                   >  1:9630829/1‑68 (MQ=255)

                                                   ggTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCaaa                 >  1:34896784/1‑68 (MQ=255)

                                                   ggTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCaaa                 >  1:495691/1‑68 (MQ=255)

                                                   ggTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCaaa                 >  1:4612150/1‑68 (MQ=255)

                                                       aaCCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTTGAACCAGGGAATGCCGGTATCAAAAAcc             >  1:25889346/1‑68 (MQ=255)

                                                       aaCCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAAcc             >  1:32557592/1‑68 (MQ=255)

                                                        aCCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCg            >  1:18244094/1‑68 (MQ=255)

                                                        aCCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCg            >  1:31117200/1‑68 (MQ=255)

                                                           gCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGt          >  1:12094081/1‑67 (MQ=255)

                                                           gCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGt          >  1:19489549/1‑67 (MQ=255)

                                                           gCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGt          >  1:6501537/1‑67 (MQ=255)

                                                           gCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGt          >  1:7610073/1‑67 (MQ=255)

                                                           gCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGt          >  1:32702505/1‑67 (MQ=255)

                                                           gCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGt          >  1:21321936/1‑67 (MQ=255)

                                                           gCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGt          >  1:14653202/1‑67 (MQ=255)

                                                            cTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGta         >  1:518632/1‑66 (MQ=255)

                                                             gtACTCGACGACGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGcc       <  1:2020631/67‑1 (MQ=11)

                                                                cTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCtt     >  1:24528233/1‑67 (MQ=255)

                                                                 tCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTa    >  1:15525575/1‑67 (MQ=255)

                                                                  cGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTAcc  >  1:18695007/1‑68 (MQ=255)

CGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACC  >  CP009273/692246‑692379

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`