| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 2,711,673 | A→C | 8.8% | V72G (GTT→GGT) | yfiF ← | putative methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,711,673 | 0 | A | C | 8.8% | 624.9 / 6.1 | 243 | V72G (GTT→GGT) | yfiF | putative methyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (165/53); new base C (3/22); total (168/75) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.33e-10 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 248 total aligned reads displayed.
AAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGG > CP009273/2711607‑2711740 | aaaCTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAAc < 1:20177620/68‑1 (MQ=255) aCTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCg > 1:26222382/1‑68 (MQ=255) aCTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCg > 1:4177143/1‑68 (MQ=255) aCTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCg > 1:24232511/1‑68 (MQ=255) aCTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCg > 1:9484150/1‑68 (MQ=255) aCTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCg > 1:18867509/1‑68 (MQ=255) cTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAACCCGt > 1:3967621/1‑68 (MQ=255) cTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGt < 1:17022934/68‑1 (MQ=255) ttttACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGt > 1:17677669/1‑67 (MQ=255) ccACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGccg < 1:10400847/68‑2 (MQ=255) ccACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGcc > 1:17285134/1‑67 (MQ=255) ccACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGcc > 1:24765188/1‑67 (MQ=255) cACTGATGCCATCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCAc > 1:26986019/1‑68 (MQ=255) cACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCAc > 1:22356947/1‑68 (MQ=255) cACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCAc > 1:14076647/1‑68 (MQ=255) cACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCAc > 1:16328799/1‑68 (MQ=255) cACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCAc > 1:648517/1‑68 (MQ=255) cACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCAc > 1:14913630/1‑68 (MQ=255) cACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCAc > 1:2358657/1‑68 (MQ=255) cACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCAc > 1:10742912/1‑68 (MQ=255) cACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCAc > 1:3445067/1‑68 (MQ=255) cACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCCCGGGAAACCGTGCGCCAc > 1:57880/1‑68 (MQ=255) cACTGAAGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCAc > 1:9736781/1‑68 (MQ=255) aCTGATGCCACCGTGCTCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGccggg < 1:1526722/68‑4 (MQ=255) aCTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGccggg < 1:18161916/68‑4 (MQ=255) ccACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCACGGCGaa > 1:22850460/1‑66 (MQ=255) ccACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCACGGCGaa > 1:16808878/1‑66 (MQ=255) ccACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCACGGCGaa > 1:314988/1‑66 (MQ=255) ccGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCtt > 1:9936125/1‑67 (MQ=255) ccGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCtt > 1:2647178/1‑67 (MQ=255) ccGTGAGCGGCCTTTTCCGGCGGCTCATCACCCGGC‑GCGCGGTAAACCGGGCGCCACGGCGAATCTTc < 1:21516139/68‑1 (MQ=255) ccGGGATCGGCCTTTCCCGGCGGCTCATCACCCGGC‑GCGCGGGAACCCGTGCGCCACGGCGAATCTTc < 1:28474786/68‑1 (MQ=255) cGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAACCCGTGCGCCACGGCGAATCTTc > 1:15404629/1‑67 (MQ=255) cGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTc > 1:10050683/1‑67 (MQ=255) cGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTc > 1:364835/1‑67 (MQ=255) cGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTc > 1:21847532/1‑67 (MQ=255) cGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTc > 1:28283043/1‑67 (MQ=255) cGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTc > 1:6057519/1‑67 (MQ=255) cGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTc > 1:28684898/1‑67 (MQ=255) gTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTccc > 1:8489261/1‑68 (MQ=255) gTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTccc > 1:27785179/1‑68 (MQ=255) gATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGGGGGAAACCGTGCGCCACGGCGAATCTTCCCAc < 1:5276479/68‑1 (MQ=255) tCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACtt > 1:4967200/1‑68 (MQ=255) tCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACtt > 1:21762582/1‑68 (MQ=255) tCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACtt > 1:25084579/1‑68 (MQ=255) tCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACtt > 1:477566/1‑68 (MQ=255) tCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACtt > 1:10574117/1‑68 (MQ=255) cGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACtt > 1:1807098/1‑67 (MQ=255) cGGCCTTTTCCGGCGGCTAATCACCCGGC‑GCGCGGTAAACCGGGCGCCACGGCGAATCTTCCCACTTg < 1:6274820/68‑1 (MQ=255) ggCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGc > 1:28766433/1‑68 (MQ=255) gCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCg > 1:2729760/1‑68 (MQ=255) gCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCg > 1:1127304/1‑68 (MQ=255) gCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTACACACTTGCg > 1:19940833/1‑68 (MQ=255) ccTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCgg > 1:19034173/1‑68 (MQ=255) cTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCgg > 1:882425/1‑67 (MQ=255) cTTATCCGGGGCCACACCCCCGGGC‑GCGGGGCAACCGGGGCGCCACGGCGAATCTTCCCACTTGCGGt < 1:25570874/68‑1 (MQ=255) ttttCCGGGGTCTCATC‑CCCGGCGGCGGGGCAACCGGTGCGCCACGGCGAATCTTCCCACTTGCGGTc < 1:7669547/68‑1 (MQ=255) ccGGCGTCTCATCCCCCGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACgg < 1:27842739/68‑1 (MQ=255) ccGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACgg > 1:20128534/1‑68 (MQ=255) ccGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACgg > 1:18965031/1‑68 (MQ=255) ccGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACgg > 1:7792375/1‑68 (MQ=255) cggcggCTCAACCCCCGGC‑GCGGGGTAACCCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACggg < 1:23912729/68‑1 (MQ=255) cGGCGTCTCATCACCCGGC‑GCGCGGTAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACggg < 1:12750252/68‑1 (MQ=255) cGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACggg > 1:9038517/1‑68 (MQ=255) cGGCGCCTAACCCCCCGGC‑GGGGGGTAACGCGGGCCCCACGGCGAATCTTCCCACTTGCGGTCACggg < 1:12564255/68‑1 (MQ=255) ggCGTCTCATCCCCCGGC‑GCGCGGCAAACGGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACgggg < 1:11942158/68‑1 (MQ=255) ggCGTCTCAACACCGGGC‑GCGGGGTAAACGGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACgggg < 1:15142206/68‑1 (MQ=255) ggCCTCTCACCCCCCGGC‑CGGCGGGAAACCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACgggg < 1:1864012/68‑1 (MQ=255) gCGTCTCATCCCCCGGC‑GCGCGGTAAACCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACgggg < 1:9674061/67‑1 (MQ=255) gCGTCCCACCACCCGGC‑GCGCGGTAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACgggg < 1:12956574/67‑1 (MQ=255) gCGGCTCATCCCCCGGC‑GCGGGGTAACCCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACgggg < 1:15757187/67‑1 (MQ=255) cGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACggg > 1:12287094/1‑65 (MQ=255) cGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACggg > 1:16080485/1‑65 (MQ=255) gTCTCATCCCCCGGC‑GCGCGGGAAACCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCt < 1:24333116/67‑1 (MQ=255) ctcATCACCCGGC‑GCGGGGCAAACCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGAc < 1:20975057/68‑1 (MQ=39) ctcAACCCCCGGC‑GCGCGGCAACCGGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGa < 1:22647024/67‑1 (MQ=255) ctcAACACCGGGC‑GCGGGGCAACCGGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGAc < 1:15029430/68‑1 (MQ=255) tcatcaCCCGGC‑GCGGGGTAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGAct < 1:26040079/68‑1 (MQ=255) gaatccCCGGGC‑GGGGGGGAAACCTTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGAct < 1:11574139/66‑1 (MQ=255) atccCCGGGC‑GGGCGGTAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGActct < 1:23185244/68‑1 (MQ=255) atcaCCCGGC‑GGGGGGCAACCCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGActct < 1:10323118/68‑1 (MQ=255) atcaCCCGGC‑GCGGGGGAACCCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGActct < 1:6893807/68‑1 (MQ=255) aCCCGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGTGTCTGtt > 1:29169200/1‑68 (MQ=255) cccGGG‑GCGCGGGAACCCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGttt < 1:2962845/68‑1 (MQ=255) cccGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGttt > 1:21971278/1‑68 (MQ=255) cccGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGttt > 1:8255890/1‑68 (MQ=255) cccGGC‑GCGCGGGAAACCGTCCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGatt > 1:16496600/1‑68 (MQ=255) ccGGC‑GCGCGGGAAACCGTGCGCCCCGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTg > 1:7039875/1‑68 (MQ=255) ccGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTg > 1:1655751/1‑68 (MQ=255) ccGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTg > 1:21232549/1‑68 (MQ=255) ccGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTg > 1:13500476/1‑68 (MQ=255) ccGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTg > 1:13052624/1‑68 (MQ=255) ccGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTg > 1:26591516/1‑68 (MQ=255) ccGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCGGACTCTGTTTg > 1:20755635/1‑68 (MQ=255) ccGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTACCCACTTGCGGTCACGGGGCTGACTCTGTTTg > 1:13611906/1‑68 (MQ=255) cGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTg > 1:5045184/1‑67 (MQ=255) gcgcgGTAAACGGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCAt < 1:25495072/67‑1 (MQ=255) gcgcgGCAAACGGTCCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATc < 1:19769134/68‑1 (MQ=255) aaCCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCgcgggcgg > 1:14654977/1‑68 (MQ=255) aCCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGcgggcggg > 1:17763579/1‑68 (MQ=255) | AAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGC‑GCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGG > CP009273/2711607‑2711740 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |