| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,462,400 | A→G | 7.1% | S271S (AGT→AGC) | chiA ← | periplasmic endochitinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,462,400 | 0 | A | G | 7.1% | 1040.7 / 27.3 | 351 | S271S (AGT→AGC) | chiA | periplasmic endochitinase |
| Reads supporting (aligned to +/- strand): ref base A (188/123); new base G (18/6); total (221/130) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.94e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 353 total aligned reads displayed.
AGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAA > CP009273/3462336‑3462467 | aGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTTCTg > 1:23869272/1‑68 (MQ=255)aGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTTCTg > 1:14041624/1‑68 (MQ=255)aGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACt > 1:12080598/1‑67 (MQ=255)aGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTg > 1:12676011/1‑68 (MQ=255)aGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTg > 1:24792792/1‑68 (MQ=255)aGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTg > 1:21233627/1‑68 (MQ=255)aGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTg > 1:27590556/1‑68 (MQ=255)aGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTg > 1:19651673/1‑68 (MQ=255)aGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTg > 1:19075421/1‑68 (MQ=255)aGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTg > 1:412836/1‑68 (MQ=255)aGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTg > 1:16812034/1‑68 (MQ=255)aGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTg > 1:8286250/1‑68 (MQ=255) ccAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGc > 1:29021450/1‑68 (MQ=255) gggTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTGCTGGCATc > 1:27767646/1‑68 (MQ=255) gggTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATc > 1:6001106/1‑68 (MQ=255) gggTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATc > 1:25375385/1‑68 (MQ=255) gggTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATc > 1:15850255/1‑68 (MQ=255) gggTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATc > 1:18442684/1‑68 (MQ=255) ggTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCg > 1:7176165/1‑68 (MQ=255) gTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGt > 1:25866574/1‑68 (MQ=255) gTTACCATACTGGCGGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATc < 1:3008406/66‑1 (MQ=255) ttACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGt < 1:17519280/67‑1 (MQ=255) ttACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGt < 1:1082226/67‑1 (MQ=255) ttACCATACTGGCTGATTTCTGGTGCCGTTGCCGCGCGAACTTAACGCCATGGGTTACTGGCATCGt < 1:26839406/67‑1 (MQ=37) aCCATACTGGCTGATTTCTGTTGCCGTTGCCGCACGAACATAACGCCATGGGTTGCTGGCATCAttgc < 1:10265104/68‑3 (MQ=17) cATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTTCTGGCATCttttggt > 1:21516252/1‑65 (MQ=21) cATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTGCTGGCATCAttttgt > 1:12302469/1‑66 (MQ=18) cATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGtttgtt > 1:15278083/1‑65 (MQ=255) cATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttct < 1:20166889/68‑1 (MQ=255) cATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttct > 1:3475255/1‑68 (MQ=255) cATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttct > 1:17912584/1‑68 (MQ=255) cATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttct > 1:23605056/1‑68 (MQ=255) cATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttct > 1:15392496/1‑68 (MQ=255) cATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttct > 1:13372315/1‑68 (MQ=255) cATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCAttttgt > 1:20798877/1‑66 (MQ=255) aTACTGGCTGATTTCTGTTGCCGTTGCCGCTCGAACATAACGCCATGGGTTGCTGGCATc < 1:6408648/60‑1 (MQ=18) aTACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttctt > 1:17278598/1‑68 (MQ=255) tACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttctt > 1:3223334/1‑67 (MQ=255) tACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttctt > 1:26465230/1‑67 (MQ=255) tACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttctt > 1:26945926/1‑67 (MQ=255) tACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttctt > 1:3981661/1‑67 (MQ=255) tACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttctt > 1:5848964/1‑67 (MQ=255) tACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttctt > 1:8023268/1‑67 (MQ=255) tACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCATtttttt > 1:15525799/1‑67 (MQ=255) aCTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTGCTGGCATCATtttgtttc > 1:25983911/1‑68 (MQ=18) aCTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttgtttc > 1:14975383/1‑68 (MQ=255) aCTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttctttc > 1:4732487/1‑68 (MQ=255) aCTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttctttc > 1:16681006/1‑68 (MQ=255) aCTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttctttc > 1:27471695/1‑68 (MQ=255) cTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCg < 1:28195183/68‑1 (MQ=255) tGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTTCTGGCATCATTGAGTTTCGc > 1:11125077/1‑68 (MQ=17) ggCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCa < 1:1154530/68‑1 (MQ=255) cTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCAt > 1:10001172/1‑67 (MQ=255) cTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATc > 1:18250734/1‑68 (MQ=255) cTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCCTTGAATTTCGCATc > 1:17706712/1‑68 (MQ=17) tGATTTGTGTTGCCGTTGCCGCGCGAACCTAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCCTCg < 1:27029385/68‑1 (MQ=25) tGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTAGTTGCATCATTTGCTTTCGcttt > 1:14300019/1‑64 (MQ=18) tGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCg < 1:16028661/68‑1 (MQ=255) tttCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCg > 1:9728063/1‑65 (MQ=255) tCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTgg < 1:10620062/68‑1 (MQ=255) gttgccgttgccGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCa > 1:14763769/1‑68 (MQ=255) tgccgttgccgCGCGAACATAACGCCATGGGTTTCTGGCATCTTTTGGTTTCGCATCGCCAGGGCagt > 1:2418495/1‑66 (MQ=18) tgccgttgccgCGCGAACATAACGCCATGGGTTTCTGGCATCGTTTTGTTTCGCATCGCCAGGGCAAt > 1:8497098/1‑68 (MQ=25) tgccgttgccgCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAAt < 1:1897863/68‑1 (MQ=255) cgttgccgCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAAttt > 1:16559612/1‑67 (MQ=255) gcgcgATCATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGcc < 1:28774293/68‑1 (MQ=255) gcgcgAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGcc < 1:27312170/68‑1 (MQ=255) gcgcgAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGcc < 1:22547070/68‑1 (MQ=255) cgcgAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCAGGGCATTTTGCCGCGCCt > 1:11214211/1‑68 (MQ=255) cgcgAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCAGGGCAATTTGCCGCGCCt > 1:22860071/1‑68 (MQ=255) gAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTAcc < 1:3315207/68‑1 (MQ=255) aCATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACcca < 1:6086462/68‑1 (MQ=255) aCATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACcca < 1:28497251/68‑1 (MQ=255) aCATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACcca < 1:13141610/68‑1 (MQ=255) aCATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACcca < 1:119586/68‑1 (MQ=255) cGCCATGGGTTACTGGCATCTTCATCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGc < 1:657133/68‑1 (MQ=38) cGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGc > 1:7383434/1‑68 (MQ=255) gCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCg > 1:13934303/1‑68 (MQ=255) gCCATGGGTTACTGGCATCGTTTTATTTCGCATCCCCAGGGCATTTAGACGAGGCTACCCACCAGGca > 1:20546138/1‑67 (MQ=9) gCCATGGGTTACTGGCATCATTTTGTTTAGCATCGCCTGGGCAGTTTGAAGAGCCTACCCACCAGGct > 1:26317984/1‑67 (MQ=14) ccATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGt > 1:24709507/1‑68 (MQ=255) ccATGGGTTACTGGCATCGTTTTCTTTCGCATCCCCAGGTCAATTAGAAGAGCCTACCCACCAGGcat > 1:11678527/1‑66 (MQ=14) ccATGGGTTACTGGCATCGTTTACTTTCGCATCGCCAGGGCACTTAGCAGCGGCTACCCACCAGGcat > 1:3784146/1‑66 (MQ=17) aTGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTc < 1:9165570/68‑1 (MQ=255) aTGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTc < 1:23227464/68‑1 (MQ=255) ttggTTACTGGCTTTGTTATCTTTGGAACCGCCTGGGCAATTTTACGCGCCTACCCACCAGGCGTTCt < 1:23823610/66‑1 (MQ=255) tGGGTTACTGGCATGGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCt < 1:18679276/68‑1 (MQ=255) tGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCt < 1:16198676/68‑1 (MQ=255) tGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCt < 1:13810909/68‑1 (MQ=255) tGGGTTACTGGCATCGTTTTCTATCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCt < 1:8774515/68‑1 (MQ=255) tGGGTTACTGGCATCGTTTTCTATCGCACCCCCTGGGCAATTTGCCGAGCCTACCCACCAGGCGTTCt < 1:529389/68‑1 (MQ=38) tGGGTTACTGGACTGTTCTGCTATCGCATCCCGTGGGCAATGTGCCGAGCCAACCCACCACGCGTTCt < 1:26642790/68‑1 (MQ=9) ggTTACTGGCATCGTTTAATTTCGCATCGCCGGGGCAGTTTGAAGAGCCTACCCACCAGGCATTTTta > 1:21055292/1‑68 (MQ=2) gTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTGTtat > 1:25146650/1‑68 (MQ=255) gTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTta < 1:17106182/67‑1 (MQ=255) gTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTta < 1:9300339/67‑1 (MQ=255) gTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTta < 1:5112565/67‑1 (MQ=255) gTTACTGGCATCGTTTTCTTTCGCATCGCCGGGGCAGTTTGAAGCGGCTACCCACCAGGCGTTTTtat > 1:6212771/1‑68 (MQ=18) tACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATaa < 1:24504377/68‑1 (MQ=255) aCTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATaaa > 1:22234409/1‑68 (MQ=255) | AGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAA > CP009273/3462336‑3462467 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |