| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 122,613 | G→A | 5.4% | K144K (AAG→AAA) | aceF → | pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 122,613 | 0 | G | A | 5.4% | 912.9 / 19.0 | 300 | K144K (AAG→AAA) | aceF | pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2 |
| Reads supporting (aligned to +/- strand): ref base G (155/119); new base A (12/4); total (173/127) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.95e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 311 total aligned reads displayed.
AATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACA > CP009273/122547‑122680 | caTCCTGGTGAACGTGCGCGACAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAgg < 1:27038147/67‑1 (MQ=11)aaTCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAgg < 1:18619292/68‑1 (MQ=255)aaTCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAg < 1:25225077/67‑1 (MQ=255) tCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGCACAGTCGCTGATCACCGTAGAAGGCGACAAGGCt > 1:27083816/1‑68 (MQ=255) tCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCTCTGATCACCGTAGAAGGCGACAAAGCt < 1:23771782/68‑1 (MQ=255) tCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCt < 1:9075684/68‑1 (MQ=255) tCCTGGTGAAAGTGGGCGATAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCt < 1:15536363/68‑1 (MQ=255) ccTGGTGAGAGTTGGCGAAAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCtt < 1:2487049/68‑1 (MQ=255) tGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCt < 1:4684139/68‑1 (MQ=255) tGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGtt < 1:25661593/67‑1 (MQ=255) ggCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTcc > 1:17836545/1‑68 (MQ=255) gATAAAGTTGAAGCTGAACAGTCTCTGATCACCGTAGAAGGCGACAAAGCtt < 1:13502620/52‑1 (MQ=14) aaaGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTcc < 1:10225077/68‑1 (MQ=255) gTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCGCCGtt > 1:28002379/1‑68 (MQ=255) tGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTg < 1:7848063/68‑1 (MQ=255) tGAAGCAGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTg < 1:17052832/68‑1 (MQ=255) gCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTg > 1:20154990/1‑67 (MQ=255) gCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCGCCGTTTGcag > 1:11040484/1‑65 (MQ=9) aaCAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCAcc > 1:245056/1‑68 (MQ=255) aCAGTCGCTGATCACCGTAGACGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCAcc < 1:28919151/67‑1 (MQ=255) aCAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCAcc < 1:24249608/67‑1 (MQ=255) aCAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCAcc < 1:22543580/67‑1 (MQ=255) aCAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCAcc < 1:24578561/67‑1 (MQ=255) aCAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCAcc < 1:11332236/67‑1 (MQ=255) aCAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCAcc < 1:27287575/67‑1 (MQ=255) aCAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCAGCg > 1:17296680/1‑68 (MQ=255) aCAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCg > 1:21087210/1‑68 (MQ=255) aCAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCg > 1:8312761/1‑68 (MQ=255) aCAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCg > 1:26835964/1‑68 (MQ=255) aCAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCg > 1:28535682/1‑68 (MQ=255) aGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGGCGTg > 1:27617433/1‑68 (MQ=255) cGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGt < 1:10506996/64‑1 (MQ=255) cGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGt < 1:19450081/64‑1 (MQ=255) cGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGt < 1:16543084/64‑1 (MQ=255) cGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGaaa > 1:7648533/1‑68 (MQ=255) cGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGaaa > 1:10558156/1‑68 (MQ=255) cGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCAGGCGGCGTGaag > 1:21521120/1‑67 (MQ=11) gCTGATCACCGTAGAAGGCGACAACGCTTCTATGGAAGTTCCGGCTCCGGTTGCGGGGAACGTTAAag > 1:21746163/1‑68 (MQ=14) tGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTg < 1:12093683/51‑1 (MQ=255) tGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAagag < 1:10812781/68‑1 (MQ=255) tGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAagag < 1:8815788/68‑1 (MQ=255) tGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAagag < 1:16432711/68‑1 (MQ=255) tGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAaga > 1:26088730/1‑67 (MQ=255) aTCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAATgaga > 1:5525365/1‑67 (MQ=255) aTCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCCCCGTGAAAgaga > 1:6838777/1‑67 (MQ=255) aTCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAgaga > 1:26424133/1‑67 (MQ=255) aTCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAgaga > 1:13086753/1‑67 (MQ=255) aTCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAgaga > 1:8581816/1‑67 (MQ=255) aTCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAgaga > 1:5393322/1‑67 (MQ=255) aTCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCGCCGTTTGCTGGCAACGTGAAAgaga > 1:20445243/1‑67 (MQ=255) aTCACCGTAGAAGGCGACAACGCGTCTATGGAAGTTCCGTCTCCGAAGGCTGGCACCGTGAAAgaga > 1:3889714/1‑67 (MQ=11) aTCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGGCTCCGTTTGCTGGCAACGTGAAAgaga > 1:14058494/1‑67 (MQ=17) tCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATc > 1:22761376/1‑68 (MQ=255) tCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCAGGCAACGTGAAGGAGATc > 1:11420064/1‑68 (MQ=14) tCACCGTAGAAGGCGACAAAGCATCTATGGAAGTTCCGGCGCCGAATGCTGGCTACGTGAAAGAGAtg > 1:16859780/1‑67 (MQ=11) cACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGtt < 1:5765940/45‑1 (MQ=255) cACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTg < 1:2083820/47‑1 (MQ=255) cACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCAcc < 1:13258951/55‑1 (MQ=255) cACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCa > 1:3788371/1‑68 (MQ=255) aCCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCCCCGTGAAAGAGATCaa > 1:14841518/1‑68 (MQ=255) aCCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATGaa > 1:2434452/1‑68 (MQ=255) ccttagtagGCTACTAGTCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCaaa < 1:15184452/65‑1 (MQ=25) ccGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCaaa < 1:4517832/68‑1 (MQ=255) cGTAGAATGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGaa < 1:1627906/57‑1 (MQ=255) gTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCCCCGTGAATGATATCAAAGt > 1:8755589/1‑68 (MQ=18) gTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCCCCGTGAAAGAGATCAAAGt > 1:1001390/1‑68 (MQ=255) gTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGt > 1:10188002/1‑68 (MQ=255) gTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGt > 1:7374524/1‑68 (MQ=255) gTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGt > 1:15639776/1‑68 (MQ=255) gTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTATGCTGGCACCGTGAAAGAGATCAAAGt > 1:14573737/1‑68 (MQ=255) gTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCGCCGTTTGCTGGCGCCGTGAAGGAGCTCAAAGt > 1:518710/1‑68 (MQ=11) gTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGTTTGCTGGCAACGTGAAAGAGATCAAAGt > 1:23078572/1‑68 (MQ=14) aGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCCGCGTGAAAGATATCAAAGtca > 1:11678040/1‑66 (MQ=18) aGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAATGAGATCAAAGTGa > 1:4031460/1‑68 (MQ=255) aGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCCCCGTGAAAGAGATCAAAGTGAACg > 1:2845525/1‑68 (MQ=255) aGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAGGAGATGAAAGTCAACg > 1:2134951/1‑68 (MQ=17) aGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGCTGAAAGTCAACg > 1:1205273/1‑68 (MQ=17) aGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACg > 1:9447612/1‑68 (MQ=255) aGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACg > 1:696442/1‑68 (MQ=255) aGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACg > 1:28078680/1‑68 (MQ=255) aGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACg > 1:21834293/1‑68 (MQ=255) aGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACg > 1:2937836/1‑68 (MQ=255) aGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCAACGTGAAAGATATCAAAGTGAACg > 1:5841666/1‑68 (MQ=255) aGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGTTTGCTGGCACCGTTAAAGAGATCAAAGTGaaa > 1:25750286/1‑66 (MQ=14) gCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGACGTGAATGAGCTCAAAGTGAACGTg > 1:13754190/1‑68 (MQ=17) gCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTg > 1:15235700/1‑68 (MQ=255) gCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTg > 1:24037919/1‑68 (MQ=255) cGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGt < 1:3395748/66‑1 (MQ=255) cGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGt < 1:1823489/66‑1 (MQ=255) aCAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGt > 1:19779760/1‑68 (MQ=255) cAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGt < 1:322617/67‑1 (MQ=255) cAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGt < 1:15935522/67‑1 (MQ=255) cAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGt < 1:6103186/67‑1 (MQ=255) cAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGt < 1:14888465/67‑1 (MQ=255) cAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGt < 1:17499005/67‑1 (MQ=255) cAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGt < 1:7928884/67‑1 (MQ=255) cAACGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGt < 1:15563813/67‑1 (MQ=255) aaGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGa < 1:12628830/68‑1 (MQ=255) aGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGAc < 1:14998155/68‑1 (MQ=255) ggCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACa > 1:12337546/1‑68 (MQ=255) | AATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACA > CP009273/122547‑122680 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |