BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,106,987	G→A	7.6%	A55V (GCC→GTC) ‡	glpK ←	glycerol kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,106,987	0	G	A	7.6%	1271.3 / 65.4	394	A55V (GCC→GTC) ‡	glpK	glycerol kinase
Reads supporting (aligned to +/- strand): ref base G (169/195); new base A (17/13); total (186/208)
Fisher's exact test for biased strand distribution p-value = 3.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 408 total aligned reads displayed.

GCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCT  >  CP009273/4106921‑4107053

gCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTgg                                                                    >  1:546476/1‑67 (MQ=255)

gCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTgg                                                                    >  1:12361092/1‑67 (MQ=255)

  tGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTggt                                                                   <  1:14526903/66‑2 (MQ=255)

  tGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTggt                                                                   <  1:12033109/66‑2 (MQ=255)

  tGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGc                                                                   <  1:27354316/66‑1 (MQ=255)

  tGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGc                                                                   <  1:1347126/66‑1 (MQ=255)

  tGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGAcc                                                                  <  1:24396495/67‑1 (MQ=255)

     aaTTTGATCGGAACTGATCTCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGa                                                              <  1:26844569/68‑1 (MQ=255)

     aaTTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGTCCAGa                                                              <  1:4922200/68‑1 (MQ=255)

     aaTTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGa                                                              <  1:6697618/68‑1 (MQ=255)

     aaTTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGa                                                              <  1:5864150/68‑1 (MQ=255)

     aaTTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGa                                                              <  1:19826859/68‑1 (MQ=255)

       tttGATCGGAACTGATCTCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGAtt                                                            >  1:25861354/1‑68 (MQ=255)

       tttGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGAtt                                                            >  1:23255385/1‑68 (MQ=255)

       tttGATCGCAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGAtt                                                            <  1:1399806/68‑1 (MQ=255)

          gATGGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTcc                                                         <  1:11753777/68‑1 (MQ=255)

          gATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGATCTTTGGGTGGCCCAGATTTcc                                                         <  1:16351027/68‑1 (MQ=255)

          gATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTcc                                                         <  1:22133381/68‑1 (MQ=255)

          gATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTcc                                                         <  1:19676615/68‑1 (MQ=255)

          gATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTcc                                                         <  1:1740737/68‑1 (MQ=255)

          gATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTcc                                                         <  1:17746436/68‑1 (MQ=255)

          gATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGACCCAGATTTcc                                                         <  1:4053659/68‑1 (MQ=255)

               gAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTgg                                                    >  1:12701811/1‑68 (MQ=255)

                aaCTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGTTTTGGGTGGCCCAGATTTCCATTgg                                                    <  1:17917307/67‑1 (MQ=255)

                aaCTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTgg                                                    <  1:8218590/67‑1 (MQ=255)

                aaCTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTgg                                                    <  1:21670512/67‑1 (MQ=255)

                aaCTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTgg                                                    <  1:21516459/67‑1 (MQ=255)

                 aCTGATCTCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTggg                                                   >  1:15757008/1‑67 (MQ=255)

                 aCTGATATCGGCTTTCGTCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTggg                                                   >  1:4411446/1‑67 (MQ=255)

                 aCTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTATATTTGGGTGGCCCATATTTCCATTGGGt                                                  <  1:20102593/68‑1 (MQ=255)

                 aCTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGt                                                  <  1:14898495/68‑1 (MQ=255)

                 aCTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGACCCAGATTTCCATTggg                                                   >  1:7954133/1‑67 (MQ=255)

                    gATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgt                                               <  1:28864212/68‑1 (MQ=255)

                    gATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGACCCAGATTTCCATTGGGTCgt                                               <  1:25600725/68‑1 (MQ=255)

                      tctCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtgt                                             >  1:20668710/3‑68 (MQ=255)

                      tctCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtgt                                             <  1:9557307/66‑1 (MQ=255)

                      tatCGGCTTTCGTCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtgt                                             >  1:17252708/1‑68 (MQ=255)

                      tatCGGCTTTCGCCAGCACTTCTACCAGCGTGGATCTTTGGGTGGCCCAGATTTCCATTGGGTCgtgt                                             >  1:5940847/1‑68 (MQ=255)

                      tatCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGTTTTGGGTGGCCCAGATTTCCATTGGGTCgtgt                                             <  1:25139540/68‑1 (MQ=255)

                      tatCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGTCCAGATTTCCATTGGGTCgtgt                                             >  1:21320040/1‑68 (MQ=255)

                      tatCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGTCCAGATTTCCATTGGGTCgtgt                                             >  1:7248652/1‑68 (MQ=255)

                      tatCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGTCCAGATTTCCATTGGGTCgtgt                                             <  1:16058942/68‑1 (MQ=255)

                      tatCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtgt                                             >  1:2944075/1‑68 (MQ=255)

                      tatCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtgt                                             <  1:28222637/68‑1 (MQ=255)

                      tatCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtgt                                             <  1:27523714/68‑1 (MQ=255)

                      tatCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtgt                                             <  1:21925467/68‑1 (MQ=255)

                      tatCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtgt                                             >  1:10134156/1‑68 (MQ=255)

                      tatCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtgt                                             <  1:4563671/68‑1 (MQ=255)

                      tatCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtgt                                             <  1:23086101/68‑1 (MQ=255)

                      tatCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtgt                                             >  1:19275178/1‑68 (MQ=255)

                      tatCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtgt                                             >  1:16576277/1‑68 (MQ=255)

                      tatCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtgt                                             >  1:8965900/1‑68 (MQ=255)

                      tatCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtg                                              <  1:2489780/67‑1 (MQ=255)

                      tatCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGCTCgtgt                                             >  1:13800947/1‑68 (MQ=255)

                      tatCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGACCCAGATTTCCATTGGGTCgtgt                                             <  1:17033787/68‑1 (MQ=255)

                         cGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCt                                          >  1:3425082/1‑68 (MQ=255)

                         cGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCt                                          <  1:2471571/68‑1 (MQ=255)

                          gggtttCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGGCGTGTTCTa                                         <  1:13243376/65‑1 (MQ=255)

                          ggCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTa                                         <  1:1151170/68‑1 (MQ=255)

                           gCTTTCGTCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTa                                         >  1:11396693/1‑67 (MQ=255)

                           gCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGTCCAGATTTCCATTGGGTCGTGTTCTa                                         >  1:12978956/1‑67 (MQ=255)

                           gCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTa                                         >  1:20495255/1‑67 (MQ=255)

                           gCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTa                                         >  1:9834470/1‑67 (MQ=255)

                           gCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTa                                         >  1:26474808/1‑67 (MQ=255)

                           gCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTa                                         >  1:14310610/1‑67 (MQ=255)

                           gCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTa                                         >  1:18406588/1‑67 (MQ=255)

                            cTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGTCCAGATTTCCATTGGGTCGTGTTCTAc                                        >  1:25232826/1‑67 (MQ=255)

                                cgtcAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCGCAGATTTCCATTGGGTCGTGTTCTACCCAAc                                   <  1:27067177/65‑1 (MQ=255)

                                cGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAAc                                   <  1:18128083/68‑1 (MQ=255)

                                cGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAAc                                   <  1:21129630/68‑1 (MQ=255)

                                  ccAGCACTTCTACCAGCGTGGATCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCaa                                    >  1:18595634/1‑65 (MQ=255)

                                  ccAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGAATTCCATTGGGTCGTGTTCTACCCAAcag                                 >  1:6973222/1‑66 (MQ=255)

                                   cAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCt                                 <  1:8410670/67‑1 (MQ=255)

                                       aCTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTgga                              <  1:13885176/66‑2 (MQ=255)

                                       aCTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGttt                            >  1:1070694/1‑68 (MQ=255)

                                       aCTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGttt                            >  1:11646019/1‑68 (MQ=255)

                                       aCTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGt                              <  1:4679478/66‑1 (MQ=255)

                                       aCTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGt                              <  1:15099727/66‑1 (MQ=255)

                                          tCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTgg                         <  1:14707987/68‑1 (MQ=255)

                                          tCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTgg                         <  1:867403/68‑1 (MQ=255)

                                          tCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTgg                         <  1:19968173/68‑1 (MQ=255)

                                             aCCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTa                      >  1:23158695/1‑68 (MQ=255)

                                             aCCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTa                      >  1:22892892/1‑68 (MQ=255)

                                              ccAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAg                     >  1:9176922/1‑68 (MQ=255)

                                              ccAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAg                     >  1:5319115/1‑68 (MQ=255)

                                              ccAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAg                     >  1:18967661/1‑68 (MQ=255)

                                              ccAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAg                     >  1:16211410/1‑68 (MQ=255)

                                              ccAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAg                     >  1:182886/1‑68 (MQ=255)

                                               cAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAgg                    <  1:15572230/68‑2 (MQ=255)

                                               cAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGa                    >  1:1101270/1‑68 (MQ=255)

                                               cAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGa                    <  1:23465572/68‑1 (MQ=255)

                                                  cGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGAttt                 >  1:18037203/1‑68 (MQ=255)

                                                  cGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGAttt                 >  1:500237/1‑68 (MQ=255)

                                                  cGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGAttt                 >  1:4856212/1‑68 (MQ=255)

                                                   gTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGt                              >  1:16828056/1‑54 (MQ=255)

                                                     ggAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGc               <  1:21830219/67‑1 (MQ=255)

                                                               gTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTcgc     >  1:6262026/1‑67 (MQ=255)

                                                               gTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTcgc     >  1:22643860/1‑67 (MQ=255)

                                                               gTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTcgc     >  1:23814004/1‑67 (MQ=255)

                                                               gTGACCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTcgc     >  1:373689/1‑67 (MQ=255)

GCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCT  >  CP009273/4106921‑4107053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`