| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 999,598 | A→C | 6.0% | T66P (ACG→CCG) | ycbF → | putative periplasmic pilin chaperone |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 999,598 | 0 | A | C | 6.0% | 1313.6 / 6.7 | 481 | T66P (ACG→CCG) | ycbF | putative periplasmic pilin chaperone |
| Reads supporting (aligned to +/- strand): ref base A (315/128); new base C (2/33); total (317/164) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.14e-15 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 484 total aligned reads displayed.
TATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTC > CP009273/999533‑999664 | tatCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAAc > 1:18101095/1‑67 (MQ=255) atCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAAcgc < 1:23942521/68‑1 (MQ=255) atCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAAcgc < 1:4529283/68‑1 (MQ=255) atCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAAcgc < 1:10019106/68‑1 (MQ=255) ttttACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTgg > 1:15251807/1‑68 (MQ=255) ttACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTggg > 1:14339129/1‑67 (MQ=255) ttACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGc < 1:12546392/68‑1 (MQ=255) aCTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCg > 1:5005411/1‑68 (MQ=255) aCTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCg > 1:18763478/1‑68 (MQ=255) aCTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCg > 1:14177688/1‑68 (MQ=255) aCTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCg > 1:13872500/1‑68 (MQ=255) aCTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCg > 1:13568719/1‑68 (MQ=255) aCTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCg > 1:28408960/1‑68 (MQ=255) aCTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCg > 1:28012556/1‑68 (MQ=255) aCTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCg > 1:1150782/1‑68 (MQ=255) aCTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCg > 1:27664045/1‑68 (MQ=255) aCTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCg > 1:26523898/1‑68 (MQ=255) aCTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCg > 1:17677703/1‑68 (MQ=255) aCTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCg > 1:24696712/1‑68 (MQ=255) aCTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGAAAAATCAACCGCCCAACGCGTTGGGCCg > 1:14006506/1‑68 (MQ=255) tGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCggg > 1:14908223/1‑68 (MQ=255) cTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCgggggg > 1:21924935/1‑68 (MQ=255) tACCAGTCAGTAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAg < 1:6623039/68‑1 (MQ=255) tACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCACCCCCCCAACGCGTTGGGCCGGGGGGGAAg < 1:8682649/68‑1 (MQ=255) tACCAGTCAGGAATCCTGGCTTATAAACAGTAAAATCAACCCCCCAACGCGTTGGGCCGGGGGGGAAg < 1:23315206/68‑1 (MQ=255) tACCAGTCAGGAATCCGGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAg < 1:21580429/68‑1 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:2935440/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:21513114/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:21141437/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:24406034/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:25126760/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:25348275/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:22532592/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:3391653/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:4586057/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:6117428/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:6869948/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:70891/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:9569134/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:9831230/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:16857357/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:11677755/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:12173660/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:17337945/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:19512814/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:12961507/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:13343157/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:15407547/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGACCAACGCGTTGGGCCGGGGGGGAAGc > 1:20082611/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACACTAAAATCAACCGCCCAACGCGTTGGCCCGGGGGGGAAGc > 1:7869194/1‑68 (MQ=255) cccgTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCg < 1:14713475/65‑1 (MQ=255) ccAGTCAGGAATCCTTGCTTATTAACACTAAAATCAACCGCCCACCGCGTTGGGCCGGGGGGGAAGCg < 1:17080419/68‑1 (MQ=255) tCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCg > 1:10811060/1‑67 (MQ=255) cAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGAc > 1:3852879/1‑68 (MQ=255) cAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGAc > 1:18887640/1‑68 (MQ=255) aaTCCTTGCTTATTAACAGTAAAATCAACCCCCCACCGCGTTGGGCCGGGGGGGAAGCGTCGACAGTg < 1:11281519/68‑1 (MQ=255) aaTCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGt < 1:19809212/67‑1 (MQ=255) aaTCCTGGCTTATTAACAGTAAAAACAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGt < 1:26022012/67‑1 (MQ=255) aTCCTGGCTTATTAACAGTAAACTCAACCGCCCACCGCGTTGGGCCGGGGGGGAAGCGTCGACAGTg < 1:16073146/67‑1 (MQ=255) aTCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTg > 1:20461247/1‑67 (MQ=255) aTCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTg > 1:21806735/1‑67 (MQ=255) aTCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTg > 1:27166731/1‑67 (MQ=255) aTCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTg > 1:26741836/1‑67 (MQ=255) aTCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTg > 1:16354559/1‑67 (MQ=255) aTCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTGGGGCCGGGGGGGAAGCGTCGACAGTg < 1:10314029/67‑1 (MQ=255) aTCCTGGCTTATTAACAGTAAAAACAACCCCCCAACGCGTGGGGCCGGGGGGGAAGCGTCGACAGTg < 1:15797055/67‑1 (MQ=255) aTCCTGGCTTATTAACAGAAAAATCACCCGCCCAACGCGTGGGGCCGGGGGGGAAGCGTCGACAGTGc < 1:261957/68‑1 (MQ=255) aTCCTGGATTATTAACAGTAAAATCAACCGCCCAACGCGTGGGGCCGGGGGGGAAGCGTCGACAGTg < 1:10611650/67‑1 (MQ=255) ccTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCa > 1:9325703/1‑68 (MQ=255) cTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGGCGGGGGGGAAGCGTCGACAGTGCCAg < 1:11016775/68‑1 (MQ=255) ggCTTATTAACCGTAAATTCCACCGCCCACCGCGTGGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCa < 1:8410477/68‑1 (MQ=255) ggCTTATTAACAGTAAAATCAACCGCCCACCGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCa < 1:20916856/68‑1 (MQ=255) ggCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCa < 1:9177985/68‑1 (MQ=255) ggCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCa < 1:6764622/68‑1 (MQ=255) ggCTTATTAACAGAAAAATCAACCCCCCACCGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCa < 1:2753188/68‑1 (MQ=255) gCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCa > 1:4127664/1‑67 (MQ=255) cTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCAcc > 1:1892152/1‑68 (MQ=255) ttatttTCAGTTAAATCAACCGGCAATCGGGGTGGGGCGGGGGGGAAGCGTCGACAGTGCGAGCAcc < 1:9036971/67‑1 (MQ=255) ttattaACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCa > 1:26347900/1‑68 (MQ=255) ttattaACAGAAAAATCACCCCCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCAcc < 1:8536169/67‑1 (MQ=255) tattaACAGTAAAAACCCCCGCCCCAGGCGGGGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCAt < 1:17945058/68‑1 (MQ=255) tattaACAGTAAAAACAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCAt < 1:3108874/68‑1 (MQ=255) taataACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCAt < 1:2314399/65‑1 (MQ=255) attaACAGTAAAATCACCCGCCCACCGCGTGGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCAtt < 1:16505247/68‑1 (MQ=255) ataaaCAGTAAAATCAACCCCCCACCGGGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCAtt < 1:548018/65‑1 (MQ=255) taACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCa > 1:28649839/1‑61 (MQ=255) taACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCAtt > 1:28896028/1‑66 (MQ=255) taACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCAtt > 1:7477471/1‑66 (MQ=255) aGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTgg > 1:22127716/1‑67 (MQ=255) gTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGcc > 1:14491286/1‑68 (MQ=255) aaaTCACCCCCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCt < 1:27809995/68‑1 (MQ=255) aaaTCAACCGCCCCACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCt < 1:23666485/68‑1 (MQ=255) aTCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTcc > 1:5862850/1‑68 (MQ=255) aTCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTcc > 1:20778643/1‑68 (MQ=255) cAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGCCAGTGCCAGCACCATTACTGGCCGCTCCg > 1:24805647/1‑67 (MQ=255) aCCTCCCATCGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCa < 1:22850744/68‑1 (MQ=255) aCCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGcc > 1:15002640/1‑67 (MQ=255) aCCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGcc > 1:223026/1‑67 (MQ=255) aCCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGcc > 1:20317387/1‑67 (MQ=255) ccACCGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTAt < 1:24281980/68‑1 (MQ=255) | TATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTC > CP009273/999533‑999664 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |