BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	692,043	T→A	7.3%	noncoding (30/75 nt)	glnV ←	tRNA‑Gln

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	692,043	0	T	A	7.3%	1028.0 / 19.7	351	noncoding (30/75 nt)	glnV	tRNA‑Gln
Reads supporting (aligned to +/- strand): ref base T (223/97); new base A (21/10); total (244/107)
Fisher's exact test for biased strand distribution p-value = 8.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 365 total aligned reads displayed.

AAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACA  >  CP009273/691976‑692110

aaGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAAt                                                                     <  1:27810506/68‑1 (MQ=255)

 aGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAAt                                                                     >  1:6923765/1‑67 (MQ=255)

  ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGaataataataa                                                                   >  1:18006366/1‑66 (MQ=21)

  ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:20203150/1‑68 (MQ=255)

  ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:4881119/1‑68 (MQ=255)

  ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:4816592/1‑68 (MQ=255)

  ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:34400411/1‑68 (MQ=255)

  ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:34196110/1‑68 (MQ=255)

  ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:33765377/1‑68 (MQ=255)

  ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:3157543/1‑68 (MQ=255)

  ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:10358333/1‑68 (MQ=255)

  ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:20633628/1‑68 (MQ=255)

  ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:30042499/1‑68 (MQ=255)

  ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:28780178/1‑68 (MQ=255)

  ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:22149561/1‑68 (MQ=255)

  ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:10234369/1‑68 (MQ=255)

  ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:24932449/1‑68 (MQ=255)

  ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:6036675/1‑68 (MQ=255)

  ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:5697286/1‑68 (MQ=255)

  ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:6619895/1‑68 (MQ=255)

  ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:14226596/1‑68 (MQ=255)

  ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:9598581/1‑68 (MQ=255)

  ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:14184281/1‑68 (MQ=255)

  ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:9046177/1‑68 (MQ=255)

  ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:11787228/1‑68 (MQ=255)

  ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:7518217/1‑68 (MQ=255)

  ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAAAcc                                                                   >  1:32562784/1‑68 (MQ=255)

  ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAAACTCTGAATGCCTGAATCAGAAAcc                                                                   >  1:24253635/1‑68 (MQ=21)

  ggTAAGCGTCTTGAATAAATTGGCTGGGCAACCACGATTCGAACCTCGGAATGCCGGTATCAGAATcc                                                                   >  1:1963616/1‑68 (MQ=21)

    tAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAAAAACCgg                                                                 >  1:13857540/1‑68 (MQ=255)

     aaGCTTCTTTATTAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGt                                                                <  1:18878641/68‑1 (MQ=14)

     aaGCGGCTTTAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGt                                                                <  1:27647991/68‑1 (MQ=255)

      aGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGt                                                                >  1:22049930/1‑67 (MQ=255)

      aGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGt                                                                >  1:11318086/1‑67 (MQ=255)

        cGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGTATCAAAAACCtgtg                                                               >  1:20887628/1‑66 (MQ=17)

        cGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTg                                                               >  1:6389306/1‑66 (MQ=255)

        cGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTg                                                               >  1:23983484/1‑66 (MQ=255)

        cGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTg                                                               >  1:17239841/1‑66 (MQ=255)

        cGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTg                                                               >  1:25790740/1‑66 (MQ=255)

        cGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTg                                                               >  1:30955981/1‑66 (MQ=255)

         gTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCt                                                            >  1:12126571/1‑68 (MQ=255)

         gTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCt                                                            >  1:7339903/1‑68 (MQ=255)

         gTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCt                                                            >  1:6829787/1‑68 (MQ=255)

         gTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGACGGAATCACAATCCGGTGCCt                                                            >  1:23147236/1‑68 (MQ=255)

         gTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCt                                                            >  1:33321872/1‑68 (MQ=11)

         cgcTTGAATAAAGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGcc                                                             <  1:18622602/65‑1 (MQ=255)

          tCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCtt                                                           >  1:2550315/1‑68 (MQ=255)

                aTAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCt                                                     >  1:16868192/1‑68 (MQ=40)

                aTAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCt                                                     >  1:9429765/1‑68 (MQ=40)

                aTAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCt                                                     >  1:16545546/1‑68 (MQ=40)

                aTAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCt                                                     >  1:25959366/1‑68 (MQ=40)

                aTAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCt                                                     >  1:30430843/1‑68 (MQ=40)

                aTAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCt                                                     >  1:5288463/1‑68 (MQ=40)

                aTAAAGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCt                                                     <  1:1596600/68‑1 (MQ=14)

                    tttGGCTGGGGTACGAGGATTCGAACCTGGGAATGCCGGTATCATAAACCGGTGCCTTACCGCTTGGc                                                 <  1:26344107/67‑1 (MQ=11)

                    tttGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGTATCACAATCCGGTGCCTTACCGCTTGGc                                                 <  1:18973081/67‑1 (MQ=11)

                    tttGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGc                                                 <  1:23906717/67‑1 (MQ=11)

                    tttGGCTGGGGTACGAGGATTCGAACCACGGAATGCCGGAATCAAAAACCGGTGCCTTACCGCTTGGc                                                 <  1:33491153/67‑1 (MQ=11)

                    aTTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                                 <  1:3941135/68‑1 (MQ=33)

                     ttGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCg                                                >  1:34028938/1‑68 (MQ=31)

                     ttGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCg                                                >  1:29490299/1‑68 (MQ=31)

                     ttGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCg                                                >  1:1074138/1‑68 (MQ=31)

                                aCGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCcaaca                                     <  1:32921420/68‑1 (MQ=31)

                                  gAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCcaacagt                                   >  1:541687/1‑66 (MQ=14)

                                   aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCcaacacgg                                  >  1:8693279/1‑65 (MQ=14)

                                   aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAGAt                                  >  1:24489410/1‑68 (MQ=14)

                                   aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAt                                  >  1:13639239/1‑68 (MQ=37)

                                   aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAt                                  >  1:22915731/1‑68 (MQ=37)

                                   aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAt                                  >  1:27202728/1‑68 (MQ=37)

                                   aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAt                                  >  1:16133751/1‑68 (MQ=37)

                                   aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAt                                  >  1:31290196/1‑68 (MQ=37)

                                   aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAt                                  >  1:3670965/1‑68 (MQ=37)

                                    ggATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAtt                                 >  1:16936190/1‑68 (MQ=39)

                                     gATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTg                                >  1:23711372/1‑68 (MQ=40)

                                     gATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTg                                >  1:819384/1‑68 (MQ=40)

                                       ttCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGt                              <  1:17638724/68‑1 (MQ=255)

                                       ttCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGt                              <  1:13214132/68‑1 (MQ=255)

                                             ccTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAGTTGGTATTgca                        >  1:23450759/1‑66 (MQ=14)

                                             ccTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGaa                        >  1:15346798/1‑68 (MQ=255)

                                              cTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAATGGTTTTGAAt                       >  1:13075832/1‑68 (MQ=255)

                                                   aaTGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGc                   <  1:30177453/67‑1 (MQ=255)

                                                    aTGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCg                 >  1:18352698/1‑68 (MQ=255)

                                                     tGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGa                <  1:14988146/68‑1 (MQ=255)

                                                        cGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAAc              <  1:7886992/67‑1 (MQ=255)

                                                         ggAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACat            <  1:29283048/68‑1 (MQ=255)

                                                            aTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACatat          <  1:31928881/67‑1 (MQ=255)

                                                            aTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACatat          <  1:974293/67‑1 (MQ=255)

                                                            aTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACatat          <  1:25164305/67‑1 (MQ=255)

                                                            aTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACatat          <  1:2694953/67‑1 (MQ=255)

                                                            aTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACatat          <  1:2854354/67‑1 (MQ=255)

                                                            aTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACatat          <  1:4307426/67‑1 (MQ=255)

                                                            aTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATAtt         <  1:12462297/68‑1 (MQ=255)

                                                             tCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTc        >  1:21448463/1‑68 (MQ=255)

                                                             tCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTc        >  1:10560590/1‑68 (MQ=255)

                                                                  aTCCGGTGCCTTACCGCTTGGCGATACCCCAACAAGTTGGTTTTGAATTGGACGAGAATATTAGATAc   >  1:22495122/1‑68 (MQ=18)

                                                                  aTCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGa      <  1:2103336/65‑1 (MQ=255)

                                                                   tCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACa  <  1:7058438/68‑1 (MQ=255)

                                                                   tCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACa  >  1:19962574/1‑68 (MQ=255)

                                                                   tCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACa  >  1:19366330/1‑68 (MQ=255)

                                                                   tCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAGTGGTTTTGAATTTGCCGATCATATTCGATACa  >  1:14478767/1‑68 (MQ=255)

AAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACA  >  CP009273/691976‑692110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`