| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 999,590 | A→C | 5.3% | N63T (AAC→ACC) | ycbF → | putative periplasmic pilin chaperone |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 999,590 | 0 | A | C | 5.3% | 1468.3 / 5.3 | 484 | N63T (AAC→ACC) | ycbF | putative periplasmic pilin chaperone |
| Reads supporting (aligned to +/- strand): ref base A (314/137); new base C (2/31); total (316/168) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.09e-13 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 501 total aligned reads displayed.
GAATCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCA > CP009273/999526‑999657 | tACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGcc > 1:14489497/1‑68 (MQ=255) aCTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCg > 1:9658641/1‑68 (MQ=255) aCTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCg > 1:19589495/1‑68 (MQ=255) aCTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCg > 1:16758366/1‑68 (MQ=255) aCTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCg > 1:15677873/1‑68 (MQ=255) tGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCACCGCGTTGGGCCggg < 1:18653098/68‑1 (MQ=255) tGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCggg < 1:321856/68‑1 (MQ=255) tGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCggg > 1:15867741/1‑68 (MQ=255) tGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCggg > 1:13238315/1‑68 (MQ=255) aCTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCggggg > 1:1063885/1‑68 (MQ=255) cTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCggtggg < 1:25523015/68‑1 (MQ=255) cTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCcgggcg > 1:24829073/1‑66 (MQ=255) aaTACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGa > 1:14067054/1‑68 (MQ=255) tACCAGTCCGGAATCCTGTCTTAATAACACTAAAATCACCCGCCCAACGCGTGGGGCCGGGGGGGAAg < 1:18212453/68‑1 (MQ=255) tACCAGTCAGGATTCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAg < 1:15373216/68‑1 (MQ=255) tACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAg < 1:6225557/68‑1 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:24393384/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:23250085/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:27874926/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:2373353/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:29543149/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:20605980/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:30641562/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:3142589/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:10875511/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:23435246/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:33571129/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:33915380/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:3651591/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:8043655/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:7639308/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:5894101/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGATGGGAAGc > 1:14916251/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCACGGGGGGAAGc > 1:20965572/1‑68 (MQ=255) ccAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCg > 1:28996965/1‑68 (MQ=255) ccAGTCAGGAATCCTGGCTTAATAACAGTAAAATCAACCCCCCAACGCGTTGGGCCGGGGGGGAAGCg < 1:17485855/68‑1 (MQ=255) tCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCg > 1:28231713/1‑67 (MQ=255) ggtaTCCTGGCTTTTTAACTGTAATATCAACCTCCCTCCGGGTTGGGCCGGGGGGGAAGCGTCGACAg < 1:5023778/65‑1 (MQ=255) aaTCCTGGCTTATTAACATTAAAATCAACCGCCCAACGCGTGGGGCCGGGGGGGAAGCGTCGACAGTg < 1:9017580/68‑1 (MQ=255) aaTCCTGGCTTATTAACATTAAAATCAACCCCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTg < 1:15494914/68‑1 (MQ=255) aaTCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGt > 1:3811366/1‑67 (MQ=255) aTCCTGGCTTATTAACAGTAAAATCAACCGCCCACCGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGc < 1:4761270/68‑1 (MQ=255) aTCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTg > 1:30415660/1‑67 (MQ=255) aTCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTg > 1:34168512/1‑67 (MQ=255) aTCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTg < 1:9460367/67‑1 (MQ=255) aTCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTg > 1:26811172/1‑67 (MQ=255) aTCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTg > 1:240967/1‑67 (MQ=255) aTCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTg > 1:1643541/1‑67 (MQ=255) aTCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTg > 1:11387950/1‑67 (MQ=255) aTCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGGTGGGCCGGGGGGGAAGCGTCGACAGTg > 1:33474323/1‑67 (MQ=255) aTCCTGGCTTATTAACAGTAAAATCAACCCCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTg < 1:2269335/67‑1 (MQ=255) aTCCTGGCTTATTAACACTAAAATCACCCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTg < 1:3575476/67‑1 (MQ=255) ccTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCa > 1:17671212/1‑68 (MQ=255) ccTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCa > 1:33015966/1‑68 (MQ=255) ccTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCa > 1:32575837/1‑68 (MQ=255) ccTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCa > 1:22990298/1‑68 (MQ=255) ccTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCa > 1:20150023/1‑68 (MQ=255) gtcTTATTAACACAAAAATCAACCCCCCAACGCGGTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCa < 1:2863635/66‑1 (MQ=255) gtattattaACACTAAAAACAACCGCCCACCGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCa < 1:1242299/65‑1 (MQ=255) ggCTTATTAACAGTAAACCCAACCGCCCACCGGGGTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCa < 1:25992628/68‑1 (MQ=255) ggCTTATTAACAGTAAAATCACCCGCCCACCGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCa < 1:2198366/68‑1 (MQ=255) ggCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCa < 1:25359120/68‑1 (MQ=255) ggCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCa < 1:19088922/68‑1 (MQ=255) ggCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCa < 1:25214389/68‑1 (MQ=255) ggCTTATTAACAGAAAAATCACCCCCCCACCGCGTGGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCa < 1:13404615/68‑1 (MQ=255) ggCTTATAAACACTAAAATCAACCGCCCACCGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCa < 1:3957482/68‑1 (MQ=255) cTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCAcc > 1:3189705/1‑68 (MQ=255) cTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCAcc > 1:19983882/1‑68 (MQ=255) cTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCAcc > 1:21347870/1‑68 (MQ=255) cTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGACAGCAcc > 1:14605119/1‑68 (MQ=255) ttattaACATTAAAATCAACCGCCCACCGCGGTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCAcc < 1:21740881/67‑1 (MQ=255) ttattaACATTAAAATCAACCGCCCAACGCGTGGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCAcc < 1:1293469/67‑1 (MQ=255) ttattaACAGTAAATTCAACCGCCCAACGCGGTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCAcc < 1:20302491/67‑1 (MQ=255) ttattaACAGTAAAATCAACCGCCCACCGCGTGGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCAcc < 1:20767876/67‑1 (MQ=255) ttattaACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCAcc < 1:13824058/67‑1 (MQ=255) tattaACCCTAAAATCAACCCCCCCCCGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCAt < 1:16163755/68‑1 (MQ=255) tataaACATTAAAACCAACCGCCCACCGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCAt < 1:7142974/68‑1 (MQ=255) taACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCAtt > 1:4851844/1‑66 (MQ=255) taACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCAtt > 1:11964742/1‑66 (MQ=255) taACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCAtt > 1:6799390/1‑66 (MQ=255) taACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCAtt > 1:6032709/1‑66 (MQ=255) taACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCAtt > 1:5521845/1‑66 (MQ=255) taACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCAtt > 1:10076449/1‑66 (MQ=255) aaCAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCAtt > 1:10967343/1‑65 (MQ=255) aCAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTg > 1:27159686/1‑68 (MQ=255) aCAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTg > 1:8405244/1‑68 (MQ=255) aGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTgg > 1:3163498/1‑67 (MQ=255) aGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTgg > 1:31166780/1‑67 (MQ=255) aGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTgg > 1:29831051/1‑67 (MQ=255) aGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTgg > 1:14250729/1‑67 (MQ=255) gTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGcc > 1:30901674/1‑68 (MQ=255) aTCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTcc > 1:19226938/1‑68 (MQ=255) aTCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTcc > 1:3015875/1‑68 (MQ=255) aTCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTcc > 1:27441609/1‑68 (MQ=255) cAACCGCCCACCGCGGTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGc < 1:8605280/68‑1 (MQ=255) cAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGc < 1:23155600/68‑1 (MQ=255) aaCCCCCCCCCGCGGGGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGcc < 1:26416921/68‑1 (MQ=255) aCCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGcc > 1:22441588/1‑67 (MQ=255) aCCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGcc > 1:4419465/1‑67 (MQ=255) aCCCCCCAACGCGTGGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCa < 1:25803297/68‑1 (MQ=255) | GAATCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCA > CP009273/999526‑999657 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |