| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 122,580 | T→C | 5.0% | A133A (GCT→GCC) | aceF → | pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 122,580 | 0 | T | C | 5.0% | 737.7 / 10.7 | 236 | A133A (GCT→GCC) | aceF | pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2 |
| Reads supporting (aligned to +/- strand): ref base T (123/95); new base C (5/7); total (129/107) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.78e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 238 total aligned reads displayed.
GGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGC > CP009273/122514‑122646 | atatCGGCAGCGACGAAGTTGAAGTGACAGAATTCATGGTGAAAGTTGGCGACAAAGTTGCAGCTGa > 1:18069091/1‑67 (MQ=14) tatCGGCAGCGACGAAGTTGAAGTGACCGAATTCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAAc > 1:1099683/1‑68 (MQ=255) tatCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGaa < 1:2342063/67‑1 (MQ=255) tatCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAAc > 1:29388272/1‑68 (MQ=255) atCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACa > 1:1178034/1‑68 (MQ=255) tCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAg > 1:24930946/1‑68 (MQ=255) tCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAg > 1:2040309/1‑68 (MQ=255) ggCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTc < 1:11204608/68‑1 (MQ=255) ggCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTc < 1:12016345/68‑1 (MQ=255) ggCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTc < 1:7435734/68‑1 (MQ=255) ggCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTc > 1:31928179/1‑68 (MQ=255) gCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTc > 1:34450730/1‑67 (MQ=255) gCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTc > 1:34138502/1‑67 (MQ=255) gCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTc > 1:24168118/1‑67 (MQ=255) gCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGAGAAAGTTGAAGCTGAACAGTc > 1:9660567/1‑67 (MQ=255) gCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTg > 1:20099704/1‑68 (MQ=255) gCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTg > 1:33407164/1‑68 (MQ=255) tgacgaAGTTGAACTGACCGATATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGa < 1:2546135/67‑1 (MQ=18) gacgaAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGa > 1:15853146/1‑67 (MQ=255) tgaAGTTGAAATCACCGAGATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATc < 1:31536592/66‑1 (MQ=11) gaAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCAc < 1:33472279/68‑1 (MQ=255) aGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCAcc < 1:26989877/67‑1 (MQ=255) aGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCg < 1:1830223/68‑1 (MQ=255) aGTTGAACTGACCGACATCCTGGTGAAAGTGGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCAcc < 1:33059940/67‑1 (MQ=14) aGTTGAACTCACCGAAATCCTGGTGAAAGTTGGCGAAAAAGTTGAAGCTGAACAGGCGCTGATCAcc < 1:31306671/67‑1 (MQ=14) tGAATTGACCGATATCCTGGTGAAAGTTGGCGATATAGTTGAAGCTGAACAGTCGCTGATCACCGt < 1:7267880/66‑1 (MQ=18) tGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGt < 1:1405148/66‑1 (MQ=255) tGAAATGACCGATATCCTGGTGAAAGTTGGCGAAAAAGTTGAAGCAGAACAGTCGCTGATCACCGt < 1:3208047/66‑1 (MQ=14) aaGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGaa > 1:9055933/1‑68 (MQ=255) aGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGAt > 1:7556766/1‑57 (MQ=255) aGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAg > 1:33838786/1‑68 (MQ=255) aGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAg > 1:33686281/1‑68 (MQ=255) aGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAg > 1:2900008/1‑68 (MQ=255) aGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAg > 1:8005717/1‑68 (MQ=255) aGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAg > 1:8384377/1‑68 (MQ=255) aGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAg > 1:13878425/1‑68 (MQ=255) aGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAg > 1:10680591/1‑68 (MQ=255) aGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAg > 1:20529069/1‑68 (MQ=255) aGTGACCGAAATCATGGTGAAAGTTGTCTATAAAATAGAAGCAGAACAGTCGAAGATCAACGTAGAAg > 1:3803807/1‑68 (MQ=255) tGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCTTACaatta > 1:29337555/1‑65 (MQ=18) tGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGc > 1:15188428/1‑68 (MQ=255) caCCGAGATCCTGGTGAAAGTTGGCGAAAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCg < 1:4786789/67‑1 (MQ=14) ccGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGAc > 1:12465794/1‑68 (MQ=255) caTCCTGGTCACAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAgg < 1:14761627/67‑1 (MQ=14) aaTCCTGGTGAAAGTTGGCGATAAAGTTGACGCTGAACAGTCACTGATCACCg < 1:20981676/53‑1 (MQ=17) aaTCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAgg < 1:31993009/68‑1 (MQ=255) aaTCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAgg < 1:5081319/68‑1 (MQ=255) aaTCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAgg < 1:21534335/68‑1 (MQ=255) aaTCCTGGTCAAAGTTGGCGACAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAgg < 1:24643050/68‑1 (MQ=255) aTCCTGGTGAACGTGGGCGACAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGc < 1:18622588/68‑1 (MQ=11) aTCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGCCAAg > 1:20778682/1‑66 (MQ=255) aTCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAg > 1:15499284/1‑66 (MQ=255) tCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCt > 1:7173598/1‑68 (MQ=255) tCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCt > 1:14246571/1‑68 (MQ=255) tCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCt < 1:31811444/68‑1 (MQ=255) tCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCt < 1:24375762/68‑1 (MQ=255) tCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCCCTGATCACCGTAGAAg < 1:33156877/57‑1 (MQ=255) tCCTGGTGAAAGTGGGCGATAAAGTTGAAGCAGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCt < 1:17647567/68‑1 (MQ=255) ccTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCtt < 1:21904659/68‑1 (MQ=255) ccTGGTGAAAGTGGGCGACAAAGTTGAAGCGGAACTGTCGCTGATCCCCGTAGAAGGCGACAAGGCtt < 1:14885910/68‑1 (MQ=11) acTGGTGTAAGTGGGCGCTCCAGTTGAAGCTGAACAGGCGATCCTCACCGTAGAAGGCGACAAGGCtt < 1:3692924/67‑1 (MQ=12) cTGGTGAAAGTTGGCGATAAAGTTGCAGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTc < 1:6910746/68‑1 (MQ=11) tGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCt < 1:4518863/68‑1 (MQ=255) ggTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTa > 1:19100069/1‑68 (MQ=255) tGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATg > 1:24762254/1‑68 (MQ=255) tGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATg > 1:959855/1‑68 (MQ=255) gAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATgg > 1:30618328/1‑68 (MQ=255) gAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATg < 1:2165039/67‑1 (MQ=255) gAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATg < 1:17956986/67‑1 (MQ=255) gAAAGTGGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATgg < 1:29230941/68‑1 (MQ=255) aaaGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGa > 1:2552210/1‑68 (MQ=255) aaaGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGa > 1:24878727/1‑68 (MQ=255) gTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGaa > 1:25292458/1‑45 (MQ=255) gTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGt > 1:28148179/1‑68 (MQ=255) tGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGtt < 1:27560332/67‑1 (MQ=255) gacaaaGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGc < 1:30459541/65‑1 (MQ=255) gATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCgg < 1:13157745/67‑1 (MQ=255) aaaGTTGAAGCGGAACAGGCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCGcc < 1:3987312/68‑1 (MQ=14) aaaGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTcc < 1:4689808/68‑1 (MQ=255) aaGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCGTCTATGGAAGTTCCGGCTCCg > 1:1574308/1‑68 (MQ=255) aGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGt > 1:22841545/1‑68 (MQ=255) tGAAGCTGAGCAGGCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTg < 1:23912861/68‑1 (MQ=255) tGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGttt > 1:15690865/1‑67 (MQ=255) tGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGttt > 1:26482647/1‑67 (MQ=255) tGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTg < 1:19666491/68‑1 (MQ=255) tGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTg < 1:26538491/68‑1 (MQ=255) tGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTg < 1:16576689/68‑1 (MQ=255) tGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTg < 1:16876421/68‑1 (MQ=255) tGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTg < 1:32707787/68‑1 (MQ=255) tGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTg < 1:653230/68‑1 (MQ=255) tGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTg < 1:5803731/68‑1 (MQ=255) tGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTg < 1:15429005/68‑1 (MQ=255) tGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCGTCTATGGAAGTTCCGGCTCCGgtt > 1:8676003/1‑67 (MQ=11) gAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGc < 1:5337668/68‑1 (MQ=255) aGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCCTCTATGGAAGTTCCGGCTCCGTTTGCTg > 1:12322273/1‑68 (MQ=255) gCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGcag > 1:16006108/1‑65 (MQ=255) gCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTg > 1:19966119/1‑67 (MQ=255) gCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTg > 1:27782089/1‑67 (MQ=255) gCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTg > 1:32907668/1‑67 (MQ=255) cTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCATCTATGGAAGTTCCGGCTCCGTTTGCTGGc > 1:28649263/1‑68 (MQ=255) | GGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGC > CP009273/122514‑122646 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |