| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 122,613 | G→A | 5.5% | K144K (AAG→AAA) | aceF → | pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 122,613 | 0 | G | A | 5.5% | 1180.9 / 29.0 | 383 | K144K (AAG→AAA) | aceF | pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2 |
| Reads supporting (aligned to +/- strand): ref base G (208/146); new base A (17/4); total (229/154) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.43e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 399 total aligned reads displayed.
AATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACA > CP009273/122547‑122680 | aTCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAg > 1:12359472/1‑66 (MQ=255) aTCCTGGTGAAAGTTGGCGATAAAATTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAg > 1:20558431/1‑66 (MQ=255) tCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCt < 1:31811444/68‑1 (MQ=255) tCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCt > 1:7173598/1‑68 (MQ=255) tCCTGGTGAAAGTTCGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCt < 1:4048944/68‑1 (MQ=255) tCCTGGTGAAAGTGGGCGATAAAGTTGAAGCAGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCt < 1:17647567/68‑1 (MQ=255) ccTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCt > 1:18796940/1‑67 (MQ=255) tGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCt < 1:9513212/68‑1 (MQ=255) tGGTCAAAGTTGGCGAAAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCt < 1:24893404/68‑1 (MQ=255) tGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATg > 1:959855/1‑68 (MQ=255) gAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATg < 1:24767826/67‑1 (MQ=255) aaaGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGa > 1:2552210/1‑68 (MQ=255) gTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGt > 1:28148179/1‑68 (MQ=255) gacaaaGTTGAAGCGGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGc < 1:8361207/65‑1 (MQ=255) aaaGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTcc < 1:4689808/68‑1 (MQ=255) aaGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCGTCTATGGAAGTTCCGGCTCCg > 1:29317/1‑68 (MQ=255) aGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGt > 1:22841545/1‑68 (MQ=255) ttGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGttt > 1:11790919/1‑68 (MQ=255) tGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGttt > 1:26482647/1‑67 (MQ=255) tGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGttt > 1:15690865/1‑67 (MQ=255) tGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTg < 1:28499895/68‑1 (MQ=255) tGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTg < 1:1483234/68‑1 (MQ=255) tGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTg < 1:19625469/68‑1 (MQ=255) tGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTg < 1:15429005/68‑1 (MQ=255) tGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTg < 1:11098539/68‑1 (MQ=255) aGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGaa > 1:23795385/1‑49 (MQ=255) gCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTg > 1:17973151/1‑67 (MQ=255) gCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTg > 1:15826190/1‑67 (MQ=255) cTGAACAGTCGCTGATCACCTTACAAGGAGACAACGCTGCTATGGAAGTTCCGTCTGGGTTTGCTGGc > 1:1600323/1‑68 (MQ=14) cTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGc > 1:13860624/1‑68 (MQ=255) tGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTgg < 1:27913222/66‑1 (MQ=255) tGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTgg < 1:27160149/66‑1 (MQ=255) aaggTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCAcc < 1:30624138/64‑1 (MQ=255) aCAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGCAGTTCCGGCTCCGTTTGCTGGCAcc < 1:14574504/67‑1 (MQ=255) aCAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCg > 1:1037879/1‑68 (MQ=255) aCAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCAcc < 1:32602511/67‑1 (MQ=255) aCAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCAcc < 1:28751187/67‑1 (MQ=255) aCAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCg > 1:1420433/1‑68 (MQ=255) aGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTg > 1:4502576/1‑68 (MQ=255) cGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGt < 1:23160126/64‑1 (MQ=255) cGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGt < 1:17392965/64‑1 (MQ=255) cGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGt < 1:25236622/64‑1 (MQ=255) cGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTg < 1:5986690/65‑1 (MQ=255) cGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGaaa > 1:18454698/1‑68 (MQ=255) cGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGaa < 1:13082090/67‑1 (MQ=255) cGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCAGGCACCGTGaac > 1:26115717/1‑67 (MQ=255) tGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAagag < 1:2950934/68‑1 (MQ=255) tGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAagag < 1:7558399/68‑1 (MQ=255) gATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAgaga < 1:33528151/68‑1 (MQ=255) aTCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAACgaga > 1:5258371/1‑67 (MQ=255) aTCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAgaga > 1:21522823/1‑67 (MQ=255) aTCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAgaga > 1:15097298/1‑67 (MQ=255) aTCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAgaga > 1:2404457/1‑67 (MQ=255) aTCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGAt < 1:31597798/68‑1 (MQ=255) aTCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGAt < 1:2203597/68‑1 (MQ=255) aTCACCGTAGAAGGCGACAAAGCGTCTATGGAAGTTCCGGCTCCGTTTGCTGGAAACGTGAAAgaga > 1:24475769/1‑67 (MQ=17) aTCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAgaga > 1:30883834/1‑67 (MQ=255) tCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAagagctg > 1:7935134/1‑65 (MQ=255) tCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCAGCGTGAATGATATc > 1:5863261/1‑68 (MQ=17) tCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATc > 1:26656907/1‑68 (MQ=255) tCACCGTAGAAGGCGACAAGGCGTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATc > 1:29886835/1‑68 (MQ=255) tCACCGTAGAAGGCGACAAGGCCTCTATGGAAGTTCCGTCTCCGGTTGCTGGGAGCGTGAAAGAGATc > 1:19909817/1‑68 (MQ=14) catccGTAGAAGGCGACAATGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGAt < 1:17112893/64‑1 (MQ=255) aCCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCaa > 1:34519612/1‑68 (MQ=255) ccGTAGAAGGCGACAAGGCTTCTATGGAATTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCaaa < 1:12634562/68‑1 (MQ=255) ccGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCaaa < 1:34115664/68‑1 (MQ=255) ccGTAGAAGGCGACAACGCATCTATGGAAGTTCCGGCTCCGTTTGCTGGAACCGTGAAAGAGATCaaa > 1:3386734/1‑68 (MQ=21) gTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCCCCGTGAAAGAGATCAAAGt > 1:32714834/1‑68 (MQ=255) aGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAACGAGCTCAAAGtca > 1:5026755/1‑66 (MQ=17) aGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGa > 1:21726430/1‑68 (MQ=255) aGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGACGTGAATGAGCTGAAAGtca > 1:30141310/1‑66 (MQ=11) aGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGa > 1:5474868/1‑68 (MQ=255) aGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGa > 1:3130914/1‑68 (MQ=255) aGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGa > 1:3299795/1‑68 (MQ=255) aGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCGCCGTTTGCTGGCACCGTGAAAGATCTCAAAGTGa > 1:30464851/1‑68 (MQ=17) aGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGATTGCTGGGAGCGTGAAAGAGATCAAAGTGa > 1:20474521/1‑68 (MQ=14) aaGGCGACAAAGCCTCTATGGAAGTTCCGGCTCCGGTTGCTGGCAACGTGAAAGAGATCAAAGTGAAc > 1:12077077/1‑68 (MQ=18) aGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACg > 1:29121395/1‑68 (MQ=255) aGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGACGTGAAGGAGATGAAAGTGAACg > 1:18130894/1‑68 (MQ=14) aGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCCCCGTGAAAGAGATCAAAGTGAACg > 1:25686205/1‑68 (MQ=255) aGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCCCCGTGAAAGAGATCAAAGTGAACg > 1:2460850/1‑68 (MQ=255) aGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCAGCGTGAAAGAGATCAAAGTGAACg > 1:3801534/1‑68 (MQ=255) aGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGATATCAAAGTGAACg > 1:32333833/1‑68 (MQ=255) aGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGCTCAAAGTGAACg > 1:9031881/1‑68 (MQ=255) aGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATGAAAGTGAACg > 1:20846608/1‑68 (MQ=255) aGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACg > 1:19341199/1‑68 (MQ=255) aGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACg > 1:14465710/1‑68 (MQ=255) aGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACg > 1:27527729/1‑68 (MQ=255) aGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACg > 1:16182067/1‑68 (MQ=255) aGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACg > 1:16829383/1‑68 (MQ=255) aGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACg > 1:16482294/1‑68 (MQ=255) aGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTCAACg > 1:3575854/1‑68 (MQ=255) aGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCAGGCGACGTGAATGAGATCAAAGTGAACg > 1:26004364/1‑68 (MQ=17) cGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGt < 1:3935752/66‑1 (MQ=255) cAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTTAAAGAGATCAAAGTGAACGTGGGt < 1:6970172/67‑1 (MQ=255) cAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGt < 1:6775276/67‑1 (MQ=255) cAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGt < 1:4916333/67‑1 (MQ=255) cAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGt < 1:21173018/67‑1 (MQ=255) aGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGAc > 1:6529358/1‑68 (MQ=255) aGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGAc < 1:30287331/68‑1 (MQ=255) | AATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACA > CP009273/122547‑122680 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |