| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,256,867 | C→G | 5.1% | E51D (GAG→GAC) | tdcD ← | propionate kinase/acetate kinase C, anaerobic |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,256,867 | 0 | C | G | 5.1% | 1439.9 / 6.3 | 438 | E51D (GAG→GAC) | tdcD | propionate kinase/acetate kinase C, anaerobic |
| Reads supporting (aligned to +/- strand): ref base C (110/302); new base G (24/2); total (134/304) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.73e-11 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 513 total aligned reads displayed.
GTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCA‑TTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATA > CP009273/3256800‑3256934 | gTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGc < 1:19562519/68‑1 (MQ=255)gTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGc < 1:9090151/68‑1 (MQ=255) tttCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGctct < 1:33317220/68‑1 (MQ=255) tCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTcc < 1:6229560/68‑1 (MQ=255) tCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTcc < 1:15177644/68‑1 (MQ=255) tCCAGATCAACAGCAAATGCCTTCCATGCACCTTCGTAGCTGTGGGGAGCCAGCGGTGCTGGCTCTcc < 1:31635037/68‑1 (MQ=255) cAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTcccc < 1:4863199/68‑1 (MQ=255) taaaaTGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCa < 1:14893749/64‑1 (MQ=255) tCAAATGCTATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCAt < 1:7335518/67‑1 (MQ=255) tCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGTCTCTCCCCCAtt < 1:7862200/68‑1 (MQ=255) tCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGGGCTCCCCCAtt > 1:27985895/1‑68 (MQ=255) tCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCa < 1:32931044/66‑1 (MQ=255) tCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCa < 1:15482168/66‑1 (MQ=255) tCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCAt < 1:25042218/67‑1 (MQ=255) tCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCAt < 1:30433332/67‑1 (MQ=255) tCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCAt < 1:21605838/67‑1 (MQ=255) cAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCAttt < 1:22338047/68‑1 (MQ=255) aaTGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTAc < 1:29303360/68‑1 (MQ=255) tGCATTTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACgg < 1:23209916/68‑1 (MQ=255) tGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGCGCCCGCGGTGCGGGGTGTCCCCCATTTAAgg > 1:10093071/1‑68 (MQ=255) tGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCGGGCGCTCCCCCATTTACg > 1:20970368/1‑67 (MQ=255) tGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGGGCTGGCTCTCCCCCAGTTAAgg > 1:18332245/1‑68 (MQ=255) aaTTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATa < 1:17856892/68‑1 (MQ=255) aaTTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATa < 1:10531510/68‑1 (MQ=255) cTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATg < 1:31302482/68‑1 (MQ=255) cTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTAAGGATAAGAAt > 1:4436883/1‑67 (MQ=255) aTGCACCTTCGTAGCTGTGGTGAGCCCGCGGTGGTGGCTCTACCCCATTTACGGATAAGAAAGCA‑ttt > 1:8188339/1‑68 (MQ=255) aCCTTCGTAGCTGTGGTGGGCCCGCGGGGGGGGGGCACCCCCCATTAAGGATTAGAAAGCA‑tttttc > 1:2671826/1‑65 (MQ=255) aCCTTCGTAGCTGTGGTGGGCCCCCGGGGCTGGGTGTCCCCCAATTAACGATTATAATTCA‑tttttag > 1:20465502/1‑65 (MQ=255) aCCTTCGTAGCTGTGGTGCGCCCGCGGGGCGGGCGCTCCCCCCTTTAAGGGTAATAATGGA‑TTTTGCg > 1:8784563/1‑68 (MQ=255) aCCTTCGTAGCTGTGGTGAGCCCGCGGGGGTGGGGCTCCCCCAATTTAGGATAATAATGGA‑atttaag > 1:1043625/1‑65 (MQ=255) ccTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCAATTACGGATAAGAATGCA‑TTTTCCGa > 1:6412707/1‑68 (MQ=255) ccTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCT‑CCCCATTTACGGATAAGAATGCA‑TATTCCGAg < 1:24570320/68‑1 (MQ=255) ccTTCGTAGCTGTGGTGAGCCACCGGTGCTGGCGGTGCCCCATTTTAGGTTAAGAATGCA‑TTTTTCGa > 1:22137143/1‑68 (MQ=255) cTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCA‑TTTTCCGAg < 1:2996598/68‑1 (MQ=255) cTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCA‑TTTTCCGAg < 1:3584384/68‑1 (MQ=255) cTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCA‑TTTTCCGAg < 1:5874411/68‑1 (MQ=255) cTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCA‑TTTTCCGAg < 1:6642404/68‑1 (MQ=255) cTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCA‑TTTTCCGAg < 1:26533710/68‑1 (MQ=255) cTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCA‑TTTTCCGAg < 1:26283487/68‑1 (MQ=255) cTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCA‑TTTTCCGAg < 1:8933385/68‑1 (MQ=255) cTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCA‑TTTTCCGAg < 1:25887682/68‑1 (MQ=255) cTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCA‑TTTTCCGAg < 1:2706467/68‑1 (MQ=255) cTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCA‑TTTTCCGAg < 1:11292893/68‑1 (MQ=255) cTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCA‑TTTTCCGAg < 1:11655598/68‑1 (MQ=255) cTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCA‑TTTTCCGAg < 1:22793652/68‑1 (MQ=255) cTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCA‑TTTTCCGAg < 1:16136179/68‑1 (MQ=255) cTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCA‑TTTTCCGAg < 1:13684984/68‑1 (MQ=255) cTTCGTAGCTGTGGGGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCA‑TTTTCCGAg < 1:31040137/68‑1 (MQ=255) cTTCGAAGCTGAGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCA‑TTTTCCGAg < 1:33181803/68‑1 (MQ=255) cTTAGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCA‑TTTTCCGAg < 1:34138062/68‑1 (MQ=255) ttCGTAGCTGTGGTGCGCCCGCGGGGGTGGGTCTCCCCCCTTTTCGGATAAGAATGCG‑TTTTGCGAGt > 1:614261/1‑68 (MQ=255) gTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCA‑TTTTCCGAGTTaa < 1:32041824/68‑1 (MQ=255) gCTGTGGTGCGCCAGCGGTGCTGGGGCTCCCCCAATTTAGGATAAGAATGCA‑TTTTACGAGTTtata > 1:19805125/1‑67 (MQ=255) ggtgAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCA‑TTTTCCGAGTTAATACCGTCg < 1:28526274/68‑1 (MQ=255) ggtgAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCA‑TTTTCCGAGTTAATACCGTCg < 1:27883989/68‑1 (MQ=255) ggtgAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCA‑TTTTCCGAGTTAATACCGTCg < 1:27714232/68‑1 (MQ=255) ggggAGCCAGCGGTGCTGGCTCGCCCCCATTTACGGATAAGAATGCA‑TTTTCCGAGTTAATACCGTCg < 1:5648917/65‑1 (MQ=255) gAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCA‑TTTTCCGAGTTAATACCGTCGGc < 1:22634980/67‑1 (MQ=255) gCCAGCGGTGGTGGCTCTCCCCCATTTACGGATAAGAATGCA‑TTTTCCGAGGTAAAACCGTCGGGAAt > 1:31542263/1‑68 (MQ=255) gCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCA‑TTTTCCGAGTTAATACCGTCGGCAAt > 1:23725103/1‑68 (MQ=255) gCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCA‑TTTTACGCGTTAATACCGTCGGCCAt > 1:34469606/1‑68 (MQ=255) gCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCA‑TTTTACGAGTTAATACCCTCGGCAAt > 1:14133915/1‑68 (MQ=255) cAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCA‑TTTTCCGAGTTAATACCGTCGGCAAt < 1:18694652/66‑1 (MQ=255) cAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCA‑TTTTCCGAGTTAATACCGTCGGCAAt < 1:6979638/66‑1 (MQ=255) cAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCA‑TTTTCCGAGTTAATACCGTCGGCAATAc < 1:29612930/68‑1 (MQ=255) cAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCA‑TTTTCCGAGTTAATACCGTCGGCAATAc < 1:12875956/68‑1 (MQ=255) cAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCA‑TTTTCCGAGTTAATACCGTCGGCAATAc < 1:8409633/68‑1 (MQ=255) cAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCA‑TTTCCCGAGTTAATACCGTCGGCAAt < 1:14596531/66‑1 (MQ=255) ggTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTTCCGAGTTAATACCGTCGGCAATAc < 1:28845637/65‑1 (MQ=255) ggTGCTGGCTCTCCCCCATTTACGGATAAGAATGCA‑TTTTCCGAGTTAATACCGTCGGCAATACCTGa < 1:30827188/68‑1 (MQ=255) ggTGCTGGCTCTCCCCCATTTACGGATAAGAATGCA‑TTTTCCGAGTTAATACCGTCGGCAATACCTGa < 1:17629631/68‑1 (MQ=255) gTGCTGGCTCTCCCCCATTTACGGATAAGAATGCA‑TTTTCCGAGTTAATACCGTCGGCAATACCTGAc > 1:9568209/1‑68 (MQ=255) gCTGGCTCTCCCCCATTTACGGATAAGAATGCA‑TTTTCCGAGTTAATACCGTCGGCAATACCTGACa < 1:33791481/67‑1 (MQ=255) gCTGGCTCTCCCCCATTTACGGATAAGAATGCA‑TTTTCCGAGTTAATACCGTCGGCAATACCTGACa < 1:17300210/67‑1 (MQ=255) gCTGGCTCTCCCCCATTTACGGATAAGAATGCA‑TTTTCCGAGTTAATACCGTCGGCAATACCTGACa < 1:3276257/67‑1 (MQ=255) gCTGGCTCTCCCCCATTTACGGATAAGAATGCA‑TTTTCCGAGTTAATACCGTCGGCAATACCTGACa < 1:20109556/67‑1 (MQ=255) gCTGGCTCTCCCCCATTTACGGATAAGAATGCA‑TTTTCCGAGTTAATACCGTCGGCAATACCTGACa < 1:13210489/67‑1 (MQ=255) gCTGGCTCTCCCCCATTTACGGATAAGAATGCA‑TTTTCCGAGTTAATACCGTCGGCAATACCTGACa < 1:31775071/67‑1 (MQ=255) gCTGGCTCTCCCCCATTTACGGATAAGAATGCA‑TTTTCCGAGTTAATACCGTCGGCAATACCTGACa < 1:17373836/67‑1 (MQ=255) gCTGGCTCTCCCCCATTTACGGATAAGAATGCA‑TTTTCCGAGTTAATACCGTCGGCAATACCTGACAt > 1:24007525/1‑68 (MQ=255) gCTGGCTCTCCCCCATTTACGGATAAGAATGCA‑TTTTCCGAGGTAATACCGTCGGCAATACCTGACa < 1:3340778/67‑1 (MQ=255) tGGCTCTCCCCCATTTACGGATAAGAATGCA‑TTTTCCGAGTTAATACCGTCGGCAATACCTGACATTa > 1:16925745/1‑68 (MQ=255) tGGCTCTCCCCCATTTACGGATAAGAATGCA‑TTTTCCGAGTTAATACCGTCGGCAATACCTGACATTa < 1:5494313/68‑1 (MQ=255) tGGCTCTCCCCCATTTACGGATAAGAATGCA‑TTTTCCGAGTTAATACCGTCGGCAATACCTGACATTa < 1:4110737/68‑1 (MQ=255) tGGCTCTCCCCCATTTACGGATAAGAATGCA‑TTTTCCGAGTTAATACCGTCGGCAATACCTGACATTa > 1:18899467/1‑68 (MQ=255) ctctCCCCCATTTACGGATATGAATGCA‑TTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAAt < 1:16522339/67‑1 (MQ=255) ctctCCCCCATTTACGGATAAGAATGCA‑TTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAAt < 1:29767476/67‑1 (MQ=255) ctctCCCCCATTTACGGATAAGAATGCA‑TTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAAt > 1:28299826/1‑67 (MQ=255) ctctCCCCCATTTACGGATAAGAATGCA‑TTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAAt > 1:28173252/1‑67 (MQ=255) ctctCCCCCATTTACGGATAAGAATGCA‑TTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAAt > 1:4680029/1‑67 (MQ=255) ctctCCCCCATTTACGGATAAGAATGCA‑TTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAAt < 1:16017143/67‑1 (MQ=255) ctctCCCCCATTTACGGATAAGAATGCA‑TTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAAt < 1:2071528/67‑1 (MQ=255) ctctCCCCCATTTACGGATAAGAATGCA‑TTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAAt > 1:1242103/1‑67 (MQ=255) ctctCCCCCATTTACGGATAAGAATGCA‑TTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAAt > 1:11987132/1‑67 (MQ=255) ctctCCCCCATTTACGGATAAGAATGCA‑TTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAAt > 1:21258255/1‑67 (MQ=255) ctctCCCCCATTTACGGATAAGAATGCA‑TTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAAt > 1:23544161/1‑67 (MQ=255) ctctCCCCCATTTACGGATAAGAATGCA‑TTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAAt < 1:25520151/67‑1 (MQ=255) ctctCCCCCATTTACGGATAAGAATGCA‑TTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAAt < 1:10865265/67‑1 (MQ=255) ctctCCCCCATTTACGGATAAGAATGCA‑TTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAAt > 1:5165031/1‑67 (MQ=255) | GTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCA‑TTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATA > CP009273/3256800‑3256934 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |