| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 161,106 | T→G | 10.0% | intergenic (+85/‑111) | hrpB → / → mrcB | putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 161,106 | 0 | T | G | 10.0% | 1468.5 / 19.4 | 476 | intergenic (+85/‑111) | hrpB/mrcB | putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase |
| Reads supporting (aligned to +/- strand): ref base T (176/234); new base G (46/2); total (240/236) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.54e-13 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 479 total aligned reads displayed.
CCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGA > CP009273/161040‑161172 | ccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTc < 1:792881/68‑1 (MQ=255) cgtgtgGGAGTGGGCCGGGGGGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCa < 1:28293119/66‑1 (MQ=37) cctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCa < 1:33634880/68‑1 (MQ=255) cctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCa < 1:27478921/68‑1 (MQ=255) cctGTGGGAGAGGGCCGGGGGGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCa < 1:7764614/68‑1 (MQ=255) cctGGGGGAGCGGGCCGGGGGGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCa < 1:6337647/68‑1 (MQ=37) tgtgGGAGAGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAg < 1:28076604/68‑1 (MQ=255) tggggggGAGGGGGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAg < 1:8123813/65‑1 (MQ=25) tgGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTGCCCCGCCCCCTGACCCTCTCCCGCAAcgg > 1:33680406/1‑68 (MQ=18) tgGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTGACCCACACCCGAACCCACGCCCGCAAggg > 1:5774050/1‑68 (MQ=18) tgGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCCCCCTCCCCCTAAACCGCTCCCGCAAggg > 1:25863346/1‑68 (MQ=21) tgGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCGCCCCCGAACCCTCTCCCGCAAggg > 1:24854600/1‑68 (MQ=21) ggtagagGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAggg < 1:25191798/64‑1 (MQ=255) gggCGAGGGCCGGGGGGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAggg < 1:22175152/67‑1 (MQ=255) gggAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAggg < 1:26495480/67‑1 (MQ=255) gggAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAggg < 1:6792916/67‑1 (MQ=255) ggAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTGACCCGCCCCCAAAACCACTCCCGCAAggggg > 1:17316043/1‑66 (MQ=17) ggAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTGAACCACACACAAAACCACAACCTCAAgggtg > 1:23757799/1‑66 (MQ=9) ggAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCCCCCGCACCCTAACCCAAGCCCGCAAgggg > 1:4394485/1‑66 (MQ=18) ggAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCGCAAGGgag > 1:6179512/1‑68 (MQ=255) ggAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCCCACCCGAACCCACACCCGCAAGGgag > 1:13753305/1‑68 (MQ=21) ggAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACACCCTAACCCTCTCCCGCCAGGgag > 1:6011363/1‑68 (MQ=25) ggAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCCCCCGCCCCCGAACCCCCGCCCCCCAggggg > 1:13149150/1‑66 (MQ=9) gatagGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGgag < 1:12361746/64‑1 (MQ=255) gagcGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGgag < 1:28868182/67‑1 (MQ=255) gagcGGGCCGGGGGGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGgag < 1:6551491/67‑1 (MQ=38) gagaGGGCCGTTGAGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCACCCTCAAGGgag < 1:4678661/67‑1 (MQ=37) gagaGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGgag < 1:14836327/67‑1 (MQ=255) gagaGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGgag < 1:19231742/67‑1 (MQ=255) agagGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCAACCAAACCCTCTCCCGCCAGGGagag > 1:9640853/1‑68 (MQ=25) agagGGCCGGGGTGAGGGCATCAGCGCGCACGTTAACCCTAACCCAAAACCACTCCCGCAAGGgagcg > 1:9202360/1‑66 (MQ=18) agGGCCGGGGTGAGGGCATCAGCGCGCACGTTGACCCGCACCCAAACCCACGCCCGCAACGGGGAggg > 1:5324168/1‑68 (MQ=18) agGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCGCCCCCGAACCCACGCCCGCAAGGGGGAggg > 1:30358022/1‑68 (MQ=21) gggCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAggg < 1:747740/67‑1 (MQ=255) gggCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAggg < 1:3632575/67‑1 (MQ=255) gggCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAggg < 1:32245984/67‑1 (MQ=255) gggCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAggg < 1:12910466/67‑1 (MQ=255) gggCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAggg < 1:15886883/67‑1 (MQ=255) gggCCGGGGGGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAggg < 1:12042775/67‑1 (MQ=255) gggCCGGGGGGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAggg < 1:14337027/67‑1 (MQ=255) gCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGa > 1:16767540/1‑67 (MQ=255) ggTGAGGGCATCAGCGCGCACGTTGACCCCCCCCCAAACCCCCTCCCGCCAGGGGGGGGGGGCCGCTc > 1:15508467/1‑68 (MQ=12) ggTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGAt < 1:25595864/67‑1 (MQ=255) ggTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAAACCTCTCCCTCAAGGGAGAGGGGCCCGATc > 1:22590170/1‑68 (MQ=255) ggTGAGGGCATCAGCGCGCACGTTCACCCGCCCCCGAACCCACGCCCGCAAGGGCGGGGGGGCCgggg > 1:1810975/1‑65 (MQ=16) gggCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGc < 1:32313277/67‑1 (MQ=255) gCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGca < 1:33911461/66‑1 (MQ=255) aTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACaaa < 1:2961654/68‑1 (MQ=255) tCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAAt < 1:32972766/68‑1 (MQ=255) aCGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGCTCGAGCACAAATTTTgagaga < 1:14584826/68‑1 (MQ=255) aCGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTgagaga < 1:16008724/68‑1 (MQ=255) aCGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTgagaga < 1:24590788/68‑1 (MQ=255) cGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGat < 1:26702804/68‑1 (MQ=255) cGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGat < 1:28625939/68‑1 (MQ=255) tcaccctcacccTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGatat < 1:9550902/67‑1 (MQ=255) tcaccctcacccTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGatat < 1:16408395/67‑1 (MQ=255) tcaccctcacccTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGatat < 1:31125280/67‑1 (MQ=255) tcaccctcacccTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGatat < 1:13448620/67‑1 (MQ=255) tcaccctcacccTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGatat < 1:27903315/67‑1 (MQ=255) tcaccctcacccTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGatat < 1:21425157/67‑1 (MQ=255) tcaccctcacccTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGatat < 1:8949598/67‑1 (MQ=255) ccctcaccctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttc < 1:11145606/68‑1 (MQ=255) cctcaccctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttct < 1:4953946/68‑1 (MQ=255) cctcaccctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttct < 1:24128949/68‑1 (MQ=255) cctcaccctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttct < 1:2337036/68‑1 (MQ=255) ccctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGt < 1:20973790/68‑1 (MQ=255) cctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttct > 1:29753685/1‑62 (MQ=255) cctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttct > 1:34055747/1‑62 (MQ=255) cctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTc < 1:9855852/68‑1 (MQ=255) cctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTc < 1:17123201/68‑1 (MQ=255) cctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTc < 1:22817051/68‑1 (MQ=255) cctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTc < 1:25281051/68‑1 (MQ=255) aaCCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTg < 1:16212727/68‑1 (MQ=255) aCCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGt > 1:3262501/1‑68 (MQ=255) aCCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGt > 1:20477390/1‑68 (MQ=255) aCCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGt > 1:27099694/1‑68 (MQ=255) cccTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTa > 1:10052077/1‑68 (MQ=255) cccTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTa < 1:8591541/68‑1 (MQ=255) ccTCTCCCTCAAGGGAGAGGGGCCCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTa > 1:11465463/1‑67 (MQ=255) ccTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTa > 1:31350763/1‑67 (MQ=255) ccTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTa > 1:26254279/1‑67 (MQ=255) ccTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTa > 1:10420774/1‑67 (MQ=255) ccTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTa > 1:10947818/1‑67 (MQ=255) ccTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTa > 1:943201/1‑67 (MQ=255) ccTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTa > 1:11639603/1‑67 (MQ=255) ccTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTa > 1:34288943/1‑67 (MQ=255) ccTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTa > 1:14015257/1‑67 (MQ=255) ccTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTa > 1:31772016/1‑67 (MQ=255) ccTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTa > 1:2449019/1‑67 (MQ=255) ccTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTa > 1:17791675/1‑67 (MQ=255) ccTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTa > 1:18320301/1‑67 (MQ=255) ccTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTa > 1:1972643/1‑67 (MQ=255) ccTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTa > 1:20675122/1‑67 (MQ=255) ccTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTa > 1:21702787/1‑67 (MQ=255) ccTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTa > 1:29182628/1‑67 (MQ=255) tctcCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACa < 1:33185615/68‑1 (MQ=255) tcCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACaga < 1:23579760/68‑1 (MQ=255) cccTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaa > 1:22291726/1‑68 (MQ=255) ccTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaag > 1:18713670/1‑68 (MQ=255) tCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTc < 1:12553965/54‑1 (MQ=255) | CCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGA > CP009273/161040‑161172 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |