| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 4,264,241 | A→G | 13.2% | K63K (AAA→AAG) | ssb → | single‑stranded DNA‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 4,264,241 | 0 | A | G | 13.2% | 1674.6 / 188.2 | 569 | K63K (AAA→AAG) | ssb | single‑stranded DNA‑binding protein |
| Reads supporting (aligned to +/- strand): ref base A (221/273); new base G (35/40); total (256/313) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.04e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.65e-01 | |||||||||||
Only 100 of 610 total aligned reads displayed.
GGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACC > CP009273/4264174‑4264310 | cGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAAc < 1:1529218/67‑1 (MQ=255) gTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTg < 1:14618970/68‑1 (MQ=255) gTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTg < 1:13585330/68‑1 (MQ=255) gATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTgg < 1:19610636/67‑1 (MQ=255) gATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTgg < 1:27106477/67‑1 (MQ=255) tAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCa < 1:225408/67‑1 (MQ=255) aaaGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGa > 1:29902349/1‑68 (MQ=255) aGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAg > 1:7095957/1‑68 (MQ=255) cGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTg > 1:32926038/1‑68 (MQ=255) ccGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGgcg > 1:34277304/1‑68 (MQ=255) cGGCGAGATGAAAGATCAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGgcga < 1:26319181/68‑1 (MQ=255) cGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGgcga < 1:19287770/68‑1 (MQ=255) cGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGgcgagc < 1:4353972/67‑1 (MQ=255) cGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGgcgagc < 1:18762407/67‑1 (MQ=255) cGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGgcgagc < 1:17862222/67‑1 (MQ=255) cGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGgcgagc < 1:28829450/67‑1 (MQ=255) cGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGgcgagc < 1:31408240/67‑1 (MQ=255) cGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGgcgagc < 1:32684826/67‑1 (MQ=255) cGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGgcgagc < 1:9075475/67‑1 (MQ=255) cGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGgcgagc < 1:14391217/67‑1 (MQ=255) cGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGgcgagc < 1:12737091/67‑1 (MQ=255) gagaTGATAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGgcgagcga < 1:27957975/68‑1 (MQ=255) agaTGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGaa > 1:26585179/1‑68 (MQ=255) tGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAata > 1:15852634/1‑67 (MQ=255) acaaCAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGACGTGGCGAGCGAATATCTGc < 1:15096320/66‑1 (MQ=255) aGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGc < 1:29523737/68‑1 (MQ=255) aGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGc < 1:17407249/68‑1 (MQ=255) aGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGc > 1:10557349/1‑68 (MQ=255) aGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGc < 1:4477498/68‑1 (MQ=255) gagcagacagaATGGCATCGCGTGGTGCTGTTCGGCAAGCTCGCGGAAGTGGCAGGTGAATGTCTGc < 1:14668155/64‑1 (MQ=255) gcagacagaATGGCATCGCGTGGTGCTGTTCGGCAAGCTCGCGGAAGTGGCAGGTGAATGTCTGCgca > 1:8688057/2‑66 (MQ=255) aCAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTa > 1:9336854/1‑68 (MQ=255) aGACAGAATGGCATCGCGTGGTGCTGTTCGGCAAGCTCGCGGAAGTGGCAGGTGAATGTCTGCgca < 1:10261779/66‑3 (MQ=255) aCTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAgg > 1:7706872/1‑68 (MQ=255) aCTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAgg > 1:6428702/1‑68 (MQ=255) aCTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAgg > 1:15495873/1‑68 (MQ=255) cTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGt < 1:5574136/68‑1 (MQ=255) cTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGt < 1:14080602/68‑1 (MQ=255) cTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGt < 1:33241630/68‑1 (MQ=255) cTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGt < 1:24695355/68‑1 (MQ=255) cTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGt > 1:2444476/1‑68 (MQ=255) cTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGt > 1:5423292/1‑68 (MQ=255) cTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGt > 1:10721822/1‑68 (MQ=255) cTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGt < 1:9734360/68‑1 (MQ=255) cTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGt < 1:2122739/68‑1 (MQ=255) cTGAATGGCACCGCGTTGTGCTGTTCGACAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGt < 1:19824220/68‑1 (MQ=255) cTGAATGGCACCGCGTTGTGCGGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGt < 1:1837687/68‑1 (MQ=255) gAATGGCATCGCGTGGTGCTGTTCGGCAAGCTCGCGGAAGTGGCAGGTGAATGTCTGCGCAAgggcgc > 1:25584550/1‑65 (MQ=255) aTGGCATCGCGTGGTGCTGTTCGGCAAGCTCGCGGAAGTGGCAGGTGAATGTCTGCGCAAgggcgc < 1:20119311/66‑4 (MQ=255) aTGGCATCGCGTGGTGCTGTTCGGCAAGCTCGCGGAAGTGGCAGGTGAATGTCTGCGCAAggg < 1:23494375/63‑1 (MQ=255) aTGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTtctc > 1:3075259/1‑68 (MQ=255) tGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCa > 1:4094543/1‑68 (MQ=255) tGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCa > 1:207905/1‑68 (MQ=255) tGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCa > 1:4959180/1‑68 (MQ=255) tGGAACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCa > 1:2325721/1‑68 (MQ=255) gCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAgg > 1:18048490/1‑68 (MQ=255) gCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAgg > 1:28267537/1‑68 (MQ=255) cACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAgg < 1:3357193/67‑1 (MQ=255) cACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAgg < 1:3912523/67‑1 (MQ=255) cACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAgg < 1:4805545/67‑1 (MQ=255) cACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAgg < 1:6614316/67‑1 (MQ=255) cACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAgg < 1:11705655/67‑1 (MQ=255) cACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAgg < 1:9841216/67‑1 (MQ=255) cACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAgg < 1:22943328/67‑1 (MQ=255) cACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAgg < 1:23841957/67‑1 (MQ=255) cACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAgg < 1:8985667/67‑1 (MQ=255) cACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAgg < 1:26852005/67‑1 (MQ=255) cACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAgg < 1:28486056/67‑1 (MQ=255) cACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAgg < 1:29198134/67‑1 (MQ=255) atcgcgTGGTGCTGTTCGGCAAGCTCGCGGAAGTGGCAGGTGAATGTCTGCGCAAGGGCGCGCAGGt < 1:21880582/65‑1 (MQ=255) aCCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGtt > 1:7365535/1‑68 (MQ=255) aCCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGtt > 1:16167194/1‑68 (MQ=255) aCCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGtt > 1:31081387/1‑68 (MQ=255) aCCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGtt > 1:20474405/1‑68 (MQ=255) aCCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGtt > 1:12249558/1‑68 (MQ=255) gcgTGGTGCTGTTCGGCAAGCTCGCGGAAGTGGCAGGTGAATGTCTGCGCAAGGGCGCGCAGGTCtac < 1:8222865/68‑2 (MQ=255) gTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTatat < 1:1718318/68‑1 (MQ=255) tgtgCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCg > 1:17046820/1‑68 (MQ=255) ggtgCTGTTCGGCAAGCTCGCGGAAGTGGCAGGTGAATGTCTGCGCAAGGGCGCGCAGGTCTACATc < 1:3183295/66‑1 (MQ=255) gtgCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGa > 1:5178706/1‑68 (MQ=255) gCTGTTCGGCAAGCTCGCGGAAGTGGCAGGTGAATGTCTGCGCAAGGGCGCGCAGGTCTACATCGAAg > 1:11383385/1‑68 (MQ=255) gCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAg > 1:6743437/1‑68 (MQ=255) gCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAg > 1:25904014/1‑68 (MQ=255) gCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAg > 1:7974772/1‑68 (MQ=255) tGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAgg < 1:10002020/67‑1 (MQ=255) tGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAgg < 1:22147457/67‑1 (MQ=255) tGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAgg < 1:24977862/67‑1 (MQ=255) tGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAgg < 1:16657678/67‑1 (MQ=255) tCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGTTCa > 1:25283269/1‑67 (MQ=255) tCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCa > 1:34521858/1‑67 (MQ=255) tCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCa > 1:21591240/1‑67 (MQ=255) tCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCa > 1:9517581/1‑67 (MQ=255) cGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGc < 1:27604391/68‑1 (MQ=255) cGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGc < 1:11948463/68‑1 (MQ=255) ggCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCt < 1:340227/68‑1 (MQ=255) ggCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCt < 1:21052589/68‑1 (MQ=255) gCAAGCTAGCGGAAGTGGCAGGTGAATGTCTGCGCAAGGGCGCGCAGGTCTACATCGAAGGTCAGCtt > 1:6041719/1‑67 (MQ=255) aaaCTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGc < 1:13163625/67‑1 (MQ=255) aaaCTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCg > 1:30500420/1‑68 (MQ=255) aaaCTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCg > 1:32009673/1‑68 (MQ=255) | GGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACC > CP009273/4264174‑4264310 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |