BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	507,925	G→A	10.7%	A277T (GCC→ACC)	glsA →	glutaminase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	507,925	0	G	A	10.7%	602.3 / 50.4	205	A277T (GCC→ACC)	glsA	glutaminase 1
Reads supporting (aligned to +/- strand): ref base G (95/88); new base A (11/11); total (106/99)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 210 total aligned reads displayed.

GGTTTACCGGGCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTCCCTGGAGTGATGGGAATTGCCGCGTTCTCACCACCGCTGGACGAAGATGGCAACAGTGTTCGCGGTCAAAAAATGGTGGCATCGGT  >  CP009273/507859‑507992

ggTTTACCGGGCAACAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTCCCTGGAGTGATGGGAATTGc                                                                    <  1:620423/68‑1 (MQ=255)

 gTTTACCGGGCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTCCCTGGAGTGATGGGAATTGcc                                                                   >  1:6509922/1‑68 (MQ=255)

  tttACCGGGCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTCCCTGGAGTGATGGGAATTGcc                                                                   >  1:21599370/1‑67 (MQ=255)

  tttACCGGGCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTCCCTGGAGTGATGGGAATTGcc                                                                   >  1:19963203/1‑67 (MQ=255)

  tttACCGGGCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTCCCTGGAGTGATGGGAATTGcc                                                                   >  1:27802070/1‑67 (MQ=255)

  tttACCGGGCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTCCCTGGAGTGATGGGAATTGcc                                                                   >  1:12588284/1‑67 (MQ=255)

  tttACCGGGCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTCCCTGGAGTGATGGGAATTGcc                                                                   <  1:3602400/67‑1 (MQ=255)

    tACCGGGCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTCCCTGGAGTGATGGGAATTGCcgcg                                                                >  1:10449894/1‑68 (MQ=255)

     aCCGGGCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTCCCTGGAGTGATGGGAATTGCCGCGt                                                               <  1:19674322/68‑1 (MQ=255)

     aCCGGGCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTCCCTGGAGTGATGGGAATTGCCGCGt                                                               <  1:31918390/68‑1 (MQ=255)

     aCCGGGCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTCCCTGGAGTGATGGGAATTGCCGCGt                                                               <  1:25856595/68‑1 (MQ=255)

     aCCGGGCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTCCCTGGAGTGATGGGAATTGCCGCGt                                                               <  1:15525378/68‑1 (MQ=255)

     aCCGGGCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTCCCTGGAGTGATGGGAATTGCCGCGt                                                               <  1:24040624/68‑1 (MQ=255)

     aCCGGGCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTCCCTGGAGTGATGGGAATTGCCGCGt                                                               <  1:8503321/68‑1 (MQ=255)

     aCCGGGCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTCCCTGGAGTGATGGGAATTGCCGCGt                                                               <  1:11641102/68‑1 (MQ=255)

     aCCGGGCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTCCCTGGAGTGATGGGAATTACCGCGt                                                               <  1:13499994/68‑1 (MQ=255)

       cGGGCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTCCCTGGAGTGATGGGAATTGCCGCGt                                                               >  1:7064252/1‑66 (MQ=255)

       cGGGCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTCCCTGGAGTGATGGGAATTGCCGCGt                                                               >  1:11943707/1‑66 (MQ=255)

       cGGGCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTCCCTGGAGTGATGGGAATTGCCGCGt                                                               >  1:28200944/1‑66 (MQ=255)

       cGGGCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTCCCTGGAGTGATGGGAATTGCCGCGt                                                               >  1:17331732/1‑66 (MQ=255)

       cGGGCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTCCCTGGAGTGATGGGAATTGCCGCGTtc                                                             <  1:15284841/68‑1 (MQ=255)

       cGGGCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTCCCTGGAGTGATGGGAATTGCCGCGTtc                                                             <  1:32221437/68‑1 (MQ=255)

       cGGGCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTCCCTGGAGTGATGGGAATTGCCGCGTtc                                                             <  1:30394509/68‑1 (MQ=255)

        gggCAAACGCGGTGTAGGTGGCGGTATTCTGGCGGGCGTCCCTGGAGTGATGGGAATTGCCGCGTtct                                                            <  1:11809813/68‑1 (MQ=255)

        gggCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTCCCTGGAGTGATGGGAATTGCCGCGTtct                                                            <  1:14444282/68‑1 (MQ=255)

        gggCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTCCCTGGAGTGATGGGAATTGCCGCGTtct                                                            <  1:313873/68‑1 (MQ=255)

           cAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTCCCTGGAGTGATGGGAATTGCCGCGTTCTca                                                          <  1:26422768/67‑1 (MQ=255)

           cAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTCCCTGGAGTGATGGGAATTGCCGCGTTCTca                                                          <  1:1684208/67‑1 (MQ=255)

             aaaGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTCCCTGGAGTGATGGGAATTACCGCGTTCTcacc                                                        >  1:4405182/1‑67 (MQ=255)

               aGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTCCCTGGAGTGATGGGAATTACCGCGTTCTCaccacc                                                     <  1:16583035/68‑1 (MQ=255)

                gCGGTGTAGGTGGCGGTATTCTGGCGGTCGTCCCTGGAGTGATGGGAATTGCCGCGTTCTCaccacc                                                     >  1:10695217/1‑67 (MQ=255)

                gCGGTGTAGGTGGCGGTATTCTGGCGGTCGTCCCTGGAGTGATGGGAATTGCCGCGTTCTCaccacc                                                     >  1:412326/1‑67 (MQ=255)

                  ggTGTAGGTGGCGGTATTCTGGCGGTCGTCCCTGGAGTGATGGGAATTGCCGCGTTCTCACCACCGc                                                   <  1:17821746/67‑1 (MQ=255)

                    tgtAGGTGGCGGTATTCTGGCGGTCGTCCCTGGAGTGATGGGAATTGCCGCGTTCTCACCACCGCTg                                                 <  1:1934363/67‑1 (MQ=255)

                    tgtAGGTGGCGGTATTCTGGCGGTCGTCCCTGGAGTGATGGGAATTGCCGCGTTCTCACCACCGCTg                                                 <  1:9282486/67‑1 (MQ=255)

                     gtAGGTGGCGGTATTCTGGCGGTCGTCCCTGGAGTGATGGGAATTGCCGCGTTCTCACCACCGCTgg                                                <  1:3387898/67‑1 (MQ=255)

                          tGGCGGTATTCTGGCGGTCGTCCCTGGAGTGATGGGAATTGCCGCGTTCTCACCACCGCTGGACGa                                            <  1:220602/66‑1 (MQ=255)

                          tGGCGGTATTCTGGCGGTCGTCCCTGGAGTGATGGGAATTGCCGCGTTCTCACCACCGCTGGACGAAg                                          >  1:31328789/1‑68 (MQ=255)

                          tGGCGGTATTCTGGCGGTCGTCCCTGGAGTGATGGGAATTGCCGCGTTCTCACCACCGCTGGACGAAg                                          >  1:24874595/1‑68 (MQ=255)

                          tGGCGGTATTCTGGCGGTCGTCCCTGGAGTGATGGGAATTGCCGCGTTCTCACCACCGCTGGACGAAg                                          >  1:23006917/1‑68 (MQ=255)

                          tGGCGGTATTCTGGCGGTCGTCCCTGGAGTGATGGGAATTGCCGCGTTCTCACCACCGCTGGACGAAg                                          >  1:22570904/1‑68 (MQ=255)

                          tGGCGGTATTCTGGCGGTCGTCCCTGGAGTGATGGGAATTGCCGCGTTCTCACCACCGCTGGACGAAg                                          >  1:18806773/1‑68 (MQ=255)

                          tGGCGGTATTCTGGCGGTCGTCCCTGGAGTGATGGGAATTGCCGCGTTCTCACCACCGCTGGACGAAg                                          >  1:19298400/1‑68 (MQ=255)

                          tGGCGGTATTCTGGCGGTCGTCCCTGGAGTGATGGGAATTGCCGCGTTCTCACCACCGCTGGACGAAg                                          >  1:13238462/1‑68 (MQ=255)

                           ggCGGTATTCTGGCGGTCGTCCCTGGAGTGATGGGAATTGCCGCGTTCTCACCACCGCTGGACGAAGa                                         <  1:14107252/68‑1 (MQ=255)

                            gCGGTATTCTGGCGGTCGTCCCTGGAGTGATGGGAATTGCCGCGTTCTCACCACCGCTGGACGAAGAt                                        >  1:6883544/1‑68 (MQ=255)

                            gCGGTATTCTGGCGGTCGTCCCTGGAGTGATGGGAATTACCGCGTTCTCCCCTCCGCTGGACGAAGAt                                        >  1:28825503/1‑68 (MQ=255)

                             cGGTATACTGGCGGTCGTCCCTGGAGTGATGGGAATTGCCGCGTACTCACCACCGCTGGACGAAGATg                                       <  1:2675890/68‑1 (MQ=255)

                              ggTATTCTGGCGGTCGTCCCTGGAGTGATGGGAATTGCCGCGTTCTCACCACCGCTGGACGAAGATg                                       >  1:33412790/1‑67 (MQ=255)

                              ggTATTCTGGCGGTCGTCCCTGGAGTGATGGGAATTGCCGCGTTCTCACCACCGCTGGACGAAGATg                                       <  1:1670399/67‑1 (MQ=255)

                              ggTATTCTGGCGGTCGTCCCTGGAGTGATGGGAATTGCCGCGTTCTCACCACCGCTGGACGAAGATg                                       <  1:18327819/67‑1 (MQ=255)

                              ggTATTCTGGCGGTCGTCCCTGGAGTGATGGGAATTGCCGCGTTCTCACCACCGCTGGACGAAGATg                                       >  1:11441177/1‑67 (MQ=255)

                              ggTATTCTGGCGGTCGTCCCTGGAGTGATGGGAATTGCCGCGTTCTCACCACCGCTGGACGAAGATg                                       <  1:33722641/67‑1 (MQ=255)

                              ggTATTCTGGCGGGCGTCCCTGGAGTGATGGGAATTGCCGCGGTCTCACCACCGCTGGACGAAGATg                                       <  1:2718899/67‑1 (MQ=255)

                               gTATTCTGGCGGTCGTCCCTGGAGTGATGGGAATTGCCGCGTTCTCACCACCGCTGGACGAAGATGGc                                     >  1:34437758/1‑68 (MQ=255)

                                tATTCTGGCGGTCGTCCCTGGAGTGATGGGAATTGCCGCGTTCTCACCACCGCTGGa                                               <  1:25341417/57‑1 (MQ=255)

                                tATTCTGGCGGTCGTCCCTGGAGTGATGGGAATTGCCGCGTTCTCACCACCGCTGGACGAAGATGGCa                                    >  1:13576107/1‑68 (MQ=255)

                                   tCTGGCGGTCGTCCCTGGAGTGATGGGAATTGCCGCGTTCTCACCACCGCTGGACGAAGATGGCAACa                                 <  1:22865748/68‑1 (MQ=255)

                                   tCTGGCGGTCGTCCCTGGAGTGATGGGAATTACCGCGTTCTCACCACCGCTGGACGAAGATGGCAACa                                 >  1:3374834/1‑68 (MQ=255)

                                     tGGCGGTCGTCCCTGGAGTGATGGGAATTGCCGCGTTCTCACCACCGCTGGACGAAGATGGCAACAgt                               <  1:4965472/68‑1 (MQ=255)

                                      ggaggTCGTCCCTGGAGTGATTGGAATTGCCGCGTTCTCACCACCGCTGGACGAATATGGCAACAgtg                              <  1:29188058/65‑1 (MQ=255)

                                      ggCGGTCGTCCCTGGAGTGATGGGAATTGCCGCGTTCTCACCACCGCTGGACGAAGATGGCAACAgtg                              >  1:20940145/1‑68 (MQ=255)

                                      ggCGGTCGTCCCTGGAGTGATGGGAATTGCCGCGTTCTCACCACCGCTGGACGAAGATGGCAACAgtg                              >  1:8125099/1‑68 (MQ=255)

                                      ggCGGTCGTCCCTGGAGTGATGGGAATTACCGCGTTCTCACCACCGCTGGACGAAGATGGCAACAgtg                              >  1:1305368/1‑68 (MQ=255)

                                        cGGTCGTCCCTGGAGTGATGGGAATTGCCGCGTTCTCACCACCGCTGGACGAAGATGGCAACAgtgt                             >  1:13710257/1‑67 (MQ=255)

                                        cGGTCGTCCCTGGAGTGATGGGAATTGCCGCGTTCTCACCACCGCTGGACGAAGATGGCAACAgtgt                             >  1:34301816/1‑67 (MQ=255)

                                        cGGTCGTCCCTGGAGTGATGGGAATTGCCGCGTTCTCACCACCGCTGGACGAAGATGGCAACAgtgt                             >  1:3512760/1‑67 (MQ=255)

                                        cGGTCGTCCCTGGAGTGATGGGAATTGCCGCGTTCTCACCACCGCTGGACGAAGATGGCAACAgtgt                             <  1:20640874/67‑1 (MQ=255)

                                               cccTGGAGTGATGGGAATTGCCGCGTTCTCACCACCGCTGGACGAAGATGGCAACAGTGTTCGCGGt                      <  1:7600001/67‑1 (MQ=255)

                                               cccTGGAGTGATGGGAATTGCCGCGTTCTCACCACCGCTGGACGAAGATGGCAACAGTGTTCGCGGt                      <  1:2144839/67‑1 (MQ=255)

                                               cccTGGAGTGATGGGAATTGCCGCGTTCTCACCACCGCTGGACGAAGATGGCAACAGTGTTCGCGGt                      <  1:22400514/67‑1 (MQ=255)

                                               cccTGGAGTGATGGGAATTGCCGCGTTCTCACCACCGCTGGACGAAGATGGCAACAGTGTTCGCGGt                      <  1:5133544/67‑1 (MQ=255)

                                               cccTGGAGTGATGGGAATTGCCGCGGTCTCACCACCGCTGGACGAAGATGGCAACAGTGTTCGCGGTc                     <  1:2811113/68‑1 (MQ=255)

                                                 cTGGAGTGATGGGAATTGCCGCGTTCTCACCACCGCTGGACGAAGATGGCAACAGTGTTCGCGGTCaa                   <  1:33115401/68‑1 (MQ=255)

                                                 cTGGAGTGATGGGAATTGCCGCGTTCTCACCACCGCTGGACGAAGATGGCAACAGTGTTCGCGGTCaa                   >  1:25562156/1‑68 (MQ=255)

                                                     aGTGATGGGAATTGCCGCGTTCTCACCACCGCTGGACGAAGATGGCAACAGTGTTCGCGGTCaataaa               >  1:9443427/1‑68 (MQ=255)

                                                     aGTGATGGGAATTGCCGCGTTCTCACCACCGCTGGACGAAGATGGCAACAGTGTTCGCGGTCaaaaaa               >  1:6442331/1‑68 (MQ=255)

                                                     aGTGATGGGAATTGCCGCGTTCTCACCACCGCTGGACGAAGATGGCAACAGTGTTCGCGGTCaaaaaa               >  1:22669036/1‑68 (MQ=255)

                                                     aGTGATGGGAATTGCCGCGTTCTCACCACCGCTGGACGAAGATGGCAACAGTGTTCGCGGTCaaaaaa               >  1:14690195/1‑68 (MQ=255)

                                                     aGTGATGGGAATTGCCGCGTTCTCACCACCGCTGGACGAAGATGGCAACAGTGTTCGCGGTCaaaaaa               >  1:9737083/1‑68 (MQ=255)

                                                     aGTGATGGGAATTGCCGCGTTCTCACCACCGCTGGACGAAGATGGCAACAGTGTTCGCGGTCaaaaaa               >  1:341973/1‑68 (MQ=255)

                                                     aGTGATGGGAATTGCCGCGTTCTCACCACCGCTGGACGAAGATGGCAACAGTGTTCGCGGTCaaaaaa               >  1:15992789/1‑68 (MQ=255)

                                                     aGTGATGGGAATTGCCGCGTTCTCACCACCGCTGGACGAAGATGGCAACAGTGTTCGCGGTCaaaaaa               >  1:23047024/1‑68 (MQ=255)

                                                     aGTGATGGGAATTGCCGCGTTCTCACCACCGCTGGACGAAGATGGCAACAGTGTTCGCGGTCaaaaaa               >  1:23647102/1‑68 (MQ=255)

                                                     aGTGATGGGAATTGCCGCGTTCTCACCACCGCTGGACGAAGATGGCAACAGTGTTCGCGGTCaaaaaa               >  1:19105095/1‑68 (MQ=255)

                                                     aGTGATGGGAATTGCCGCGTTCTCACCACCGCTGGACGAAGATGGCAACAGTGTTCGCGGTCaaaaaa               >  1:20154122/1‑68 (MQ=255)

                                                     aGTGATGGGAATTGCCGCGTTCTCACCACCGCTGGACGAAGATGGCAACAGTGTTCGCGGTCaaaaaa               >  1:26872709/1‑68 (MQ=255)

                                                     aGTGATGGGAATTGCCGCGTTCTCACCACCGCTGGACGAAGATGGCAACAGTGTTCGCGGTCaaaaaa               >  1:20427099/1‑68 (MQ=255)

                                                     aGTGATGGGAATTGCCGCGTTCTCACCACCGCTGGACGAAGATGGCAACAGTGATCGCGGTCaaaaaa               >  1:27535462/1‑68 (MQ=255)

                                                     aGTGATGGGAATTACCGCGTTCTCACCACCGCTGGACGAAGATGGCAACAGTGTTCGCGGTCaaaaaa               >  1:16247495/1‑68 (MQ=255)

                                                     aGTGATGGGAATTACCGCGTTCTCACCACCGCTGGACGAAGATGGCAACAGTGTTCGCGGTCaaaaaa               >  1:5304587/1‑68 (MQ=255)

                                                     aGTGATGGGAATTACCGCGTTCTCACCACCGCTGGACGAAGATGGCAACAGTGTTCGCGGTCaaaaaa               >  1:29905899/1‑68 (MQ=255)

                                                     aGTGATGGGAATTACCGCGTTCTCACCACCGCTGGACGAAGATGGCAACAGTGTTCGCGGTCaaaaa                <  1:8791247/67‑1 (MQ=255)

                                                        gATGGGAATTGCCGCGTTCTCACCACCGCTGGACGAAGATGGCAACAGTGTTCGCGGTCAAAAAAtg             <  1:15873681/67‑1 (MQ=255)

                                                          tGGGAATTGCCGCGTTCTCACCACCGCTGGACGAAGATGGCAACAGTGTTCGCGGTCAAAAAAtggtg          >  1:32702880/1‑68 (MQ=255)

                                                           gggAATTGCCGCGTTCTCACCACCGCTGGACGAAGATGGCAACAGTGTTCGCGGTCAAAAAAtggtgg         <  1:24523284/68‑1 (MQ=255)

                                                           gggAATTGCCGCGTTCTCACCACCGCTGGACGAAGATGGCAACAGTGTTCGCGGTCAAAAAAtggtgg         <  1:33479998/68‑1 (MQ=255)

                                                           gggAATTGCCGCGTTCTCACCACCGCTGGACGAAGATGGCAACAGTGTTCGCGGTCAAAAAAtggtg          >  1:2252366/1‑67 (MQ=255)

                                                           gggAATTACCGCGTTCTCACCACCGCTGGACGAAGATGGCAACAGTGTTCGCGGTCAAAAAAtggtgg         <  1:7306276/68‑1 (MQ=255)

                                                               aTTGCCGCGTTCTCACCACCGCTGGACGAAGATGGCAACAGTGTTCGCGGTCAAAAAATGGTGGCATc     >  1:23697957/1‑68 (MQ=255)

GGTTTACCGGGCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTCCCTGGAGTGATGGGAATTGCCGCGTTCTCACCACCGCTGGACGAAGATGGCAACAGTGTTCGCGGTCAAAAAATGGTGGCATCGGT  >  CP009273/507859‑507992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`