| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 66,706 | T→C | 63.1% | intergenic (‑174/‑168) | araD ← / → araC | pseudogene, truncated/ara regulon transcriptional activator; autorepressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 66,706 | 0 | T | C | 63.1% | 1006.7 / inf | 1099 | intergenic (‑174/‑168) | araD/araC | pseudogene, truncated/ara regulon transcriptional activator; autorepressor |
| Reads supporting (aligned to +/- strand): ref base T (230/174); new base C (383/307); total (617/482) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.59e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.51e-01 | |||||||||||
Only 100 of 1100 total aligned reads displayed.
ATAGCAAAGTGTGACGCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGTTACGCGTTTTTGTCATGGCTTTGGTCCCGCTTTGTTACAGAATGCTTTTAATAAGCGGGGTTACCG > CP009273/66639‑66772 | cAAAGTGTGACGCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGTTAc > 1:645748/1‑67 (MQ=255) cAAAGTGTGACGCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGCTAc > 1:81481/1‑67 (MQ=255) cAAAGTGTGACGCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGCTAc > 1:18024369/1‑67 (MQ=255) cAAAGTGTGACGCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGCTAc > 1:25819201/1‑67 (MQ=255) aaaGTGTGACGCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGTTAcgc > 1:20325648/1‑68 (MQ=255) aaaGTGTGACGCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGTTAcgc > 1:12667815/1‑68 (MQ=255) aaaGTGTGACGCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGTTAcgc > 1:22839434/1‑68 (MQ=255) aaaGTGTGACGCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGCTAcgc > 1:16358199/1‑68 (MQ=255) aaaGTGTGACGCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGCTAcgc > 1:20145759/1‑68 (MQ=255) aaaGTGTGACGCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGCTAcgc > 1:10106855/1‑68 (MQ=255) aaaGTGTGACGCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGCTAcgc < 1:29229702/68‑1 (MQ=255) aaaGTGTGACGCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGCTAcgc < 1:21038202/68‑1 (MQ=255) aGTGTGACGCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGt > 1:8814617/1‑68 (MQ=255) gtgtGACGCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGtt > 1:31471155/1‑68 (MQ=255) tgtgACGCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGTTACGCGttt > 1:11750553/1‑68 (MQ=255) tgtgACGCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGttt > 1:5822072/1‑68 (MQ=255) gtgACGCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGTTACGCGtttt > 1:13936023/1‑68 (MQ=255) gACGCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTg < 1:9390321/68‑1 (MQ=255) gACGCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTg > 1:32106194/1‑68 (MQ=255) gCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGTTACGCGTTTTTGTCa > 1:17125750/1‑68 (MQ=255) gCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGTTACGCGTTTTTGTCa > 1:8505364/1‑68 (MQ=255) gCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGTTACGCGTTTTTGTCa > 1:30725039/1‑68 (MQ=255) gCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGTTACGCGTTTTTGTCa > 1:10448895/1‑68 (MQ=255) gCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGTTACGCGTTTTTATCa > 1:21458607/1‑68 (MQ=255) gCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGTTACGAGTTTTTGTCa > 1:1421918/1‑68 (MQ=255) gCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCa > 1:2739707/1‑68 (MQ=255) gCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCa > 1:18248064/1‑68 (MQ=255) gCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCa > 1:10676501/1‑68 (MQ=255) gCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCa > 1:25326472/1‑68 (MQ=255) gCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCa > 1:15668767/1‑68 (MQ=255) gCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCa > 1:9115724/1‑68 (MQ=255) gCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCa > 1:14780792/1‑68 (MQ=255) gCCGTGCAAATAATCAAGGTGGACTTTTCTGCCGTGAATATAGACACTTTTGCTACGCGTTTTTGTCa > 1:22183502/1‑68 (MQ=255) cGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGTTACGCGTTTTTGTCATg > 1:26534836/1‑68 (MQ=255) cGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCATg < 1:3967907/68‑1 (MQ=255) gCAAATAATCAATGTGGACTTTTCTGCCTTGATTATAGACACTTTTGTTACGCGTTTTTGTCATGGCt < 1:14554506/68‑1 (MQ=255) gCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCATGGcc < 1:27183025/68‑2 (MQ=255) taatCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGTTACGCGTTTTTGTCATGGCTTTgg > 1:28275700/1‑67 (MQ=255) taatCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGTTACGCGTTTTTGTCATGGCTTTgg > 1:6866618/1‑67 (MQ=255) taatCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGTTACGCGTTTTTGTCATGGCTTTGGt < 1:6324613/68‑1 (MQ=255) taatCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCATGGCCTTgg > 1:13644147/1‑67 (MQ=255) tCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCATGGCCTTGGTccc > 1:21873771/1‑68 (MQ=255) tCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCATGGCCTTGGTccc > 1:7918900/1‑68 (MQ=255) tCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCATGGCCTTGGTccc > 1:7554848/1‑68 (MQ=255) aTGTGGACTTTTCTGCCGTGATTATAGACACTTTTGTTACGCGTTTTTGTCATGGCTTTGGTCCCGCt < 1:4174453/68‑1 (MQ=255) aTGTGGACTTTTCTGCCGTGATTATAGACACTTTTGTTACGCGTTTTTGTCATGGCTTTGGTCCCGCt < 1:22430407/68‑1 (MQ=255) aTGTGGACTTTTCTGCCGTGATTATAGACACTTTTGCTACGGGTTTTTGTCATGGCCTTGGTCGCGCt < 1:26012119/68‑1 (MQ=255) aTGTGGACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCt < 1:20774037/68‑1 (MQ=255) aTGTGGACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCt < 1:16607411/68‑1 (MQ=255) aTGTGGACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCt < 1:20534306/68‑1 (MQ=255) tgtgGACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCtt > 1:31768981/1‑68 (MQ=255) tgtgGACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCtt > 1:2353593/1‑68 (MQ=255) tgtgGACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCtt > 1:5968441/1‑68 (MQ=255) gtgGACTTTTCTGCCGTGATTATAGACACTTTTGTTACGCGTTTTTGTCATGGCTTTGGTCCCGCttt < 1:26841474/68‑1 (MQ=255) gtgGACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCttt < 1:16814318/68‑1 (MQ=255) gtgGACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCttt < 1:11023512/68‑1 (MQ=255) gtgGACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCttt < 1:11355743/68‑1 (MQ=255) gtgGACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCttt < 1:27730994/68‑1 (MQ=255) tgACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCTTTGt < 1:30249770/67‑1 (MQ=255) ggACTTTTCTGCCGTGATTATAGACACTTTTGTTACGCGTTTTTGTCATGGCTTTGGTCCCGCTTTGt < 1:19727619/68‑1 (MQ=255) ggACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCTTTGt < 1:13336046/68‑1 (MQ=255) ggACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCTTTGt < 1:25036048/68‑1 (MQ=255) ggACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCTTTGt < 1:4480915/68‑1 (MQ=255) ggACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCTTTGt < 1:24131025/68‑1 (MQ=255) ggACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCTTTGt < 1:17795552/68‑1 (MQ=255) cTTTTCTGCCGTGATTATAGACACTTTTGTTACGCGTTTTTGTCATGGCTTTGGTCCCGCTTTGTTAc > 1:27921080/1‑68 (MQ=255) cTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCTTTGTTAc > 1:28703925/1‑68 (MQ=255) cTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCTTTGTTAc > 1:9503083/1‑68 (MQ=255) cTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCTTTGTTAc > 1:19991849/1‑68 (MQ=255) tttCTGCCGTGATTATAGACACTTTTGTTACGCGTTTTTGTCATGGCTTTGGTCCCGCTTTGTTACAg > 1:15022475/1‑68 (MQ=255) gCCGTGATTATAGACACTTTTGTTACGCGTTTTTGTCATGGCTTTGGTCCCGCTTTGTTACAGAATGc < 1:24477520/68‑1 (MQ=255) gCCGTGATTATAGACACTTTTGTTACGCGTTTTTGTCATGGCTTTGGTCCCGCTTTGTTACAGAATGc < 1:4982757/68‑1 (MQ=255) gCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCTTTGTTACAGAATGc < 1:548040/68‑1 (MQ=255) gCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCTTTGTTACAGAATGc < 1:4784194/68‑1 (MQ=255) gCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCTTTGTTACAGAATGc < 1:18835820/68‑1 (MQ=255) cGTGATTATAGACACTTTTGTTACGCGTTTTTGTCATGGCTTTGGTCCCGCTTTGTTACAGAATGCt < 1:15994103/67‑1 (MQ=255) cGTGATTATAGACACTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCTTTGTTACAGAATGCtt > 1:19084873/1‑68 (MQ=255) cGTGATTATAGACACTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCTTTGTTACAGAATGCt < 1:9631776/67‑1 (MQ=255) cGTGATTATAGACACTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCTTTGTTACAGAATGCt < 1:25566759/67‑1 (MQ=255) gTGATTATAGACACTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCTTTGTTACAGAATGCttt < 1:29608143/68‑1 (MQ=255) tGATTATAGACACTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCTTTGTTACAGAATGCtttt > 1:3480072/1‑68 (MQ=255) gATTATAGACACTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCTTTGTTACAGAATGCTTTta < 1:23894740/68‑1 (MQ=255) ttATAGACACTTTTGTTACGCGTTTTTGTCATGGCTTTGGTCCCGCTTTGTTACAGAATGCTTTtaat > 1:15454905/1‑68 (MQ=255) gACACTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCTTTGTTACAGAATGCTTTTAATAAGCg > 1:19492478/1‑68 (MQ=255) acacTTTTGTTACGCGTTTTTGTCATGGCTTTGGTCCCGCTTTGTTACAGAATGCTTTTAATAAGCgg > 1:5215801/1‑68 (MQ=255) acacTTTTGTTACGCGTTTTTGTCATGGCTTTGGTCCCGCTTTGTTACAGAATGCTTTTAATAAGCgg > 1:31216658/1‑68 (MQ=255) acacTTTTGTTACGCGTTTTTGTCATGGCTTTGGTCCCGCTTTGTTACAGAATGCTTTTAATAAGCgg > 1:24696568/1‑68 (MQ=255) acacTTTTGTTACGCGTTTTTGTCATGGCTTTGGTCCCGCTTTGTTACAGAATGCTTTTAATAAGCgg > 1:12216610/1‑68 (MQ=255) acacTTTTGTTACGCGTTTTTGTCATGGCTTTGGTCCCGCTTTGTTACAGAATGCTTTTAATAAGCgg > 1:13017066/1‑68 (MQ=255) acacTTTTGTTACGCGTTTTTGTCATGGCTTTGGTCCCGCTTTGTTACAGAATGCTTTTAATAAGCgg > 1:17524580/1‑68 (MQ=255) acacTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCTTTGTTACAGAATGCTTTTAATAAGCgg > 1:30523253/1‑68 (MQ=255) acacTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCTTTGTTACAGAATGCTTTTAATAAGCgg > 1:29016014/1‑68 (MQ=255) acacTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCTTTGTTACAGAATGCTTTTAATAAGCgg > 1:26377051/1‑68 (MQ=255) cacTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCTTTGTTACAGAATGCTTTTAATAAGCggg > 1:7204101/1‑68 (MQ=255) cacTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCTTTGTTACAGAATGCTTTTAATAAGCggg > 1:23254268/1‑68 (MQ=255) cacTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCTATGTTACAGAATGCTTTTAATAAGCggg < 1:18614368/68‑1 (MQ=255) acTTTTGTTACGCGTTTTTGTCATGGCTTTGGTCCCGCTTTGTTACAGAATGCTTTTAATAAGCgggg < 1:23809358/68‑1 (MQ=255) ttttGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCTTTGTTACAGAATGCTTTTAATAAGCGGGGtt > 1:29952392/1‑68 (MQ=255) gTTACGCGTTTTTGTCATGGCTTTGGTCCCGCTTTGTTACAGAATGCTTTTAATAAGCGGGGTTACCg > 1:366809/1‑68 (MQ=255) gTTACGCGTTTTTGTCATGGCTTTGGTCCCGCTTTGTTACAGAATGCTTTTAATAAGCGGGGTTACCg > 1:11948566/1‑68 (MQ=255) | ATAGCAAAGTGTGACGCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGTTACGCGTTTTTGTCATGGCTTTGGTCCCGCTTTGTTACAGAATGCTTTTAATAAGCGGGGTTACCG > CP009273/66639‑66772 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |