| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 1,243,621 | C→T | 7.3% | G317D (GGT→GAT) | dhaM ← | putative dihydroxyacetone‑specific PTS enzymes: HPr component/EI component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 1,243,621 | 0 | C | T | 7.3% | 1846.7 / 96.2 | 566 | G317D (GGT→GAT) | dhaM | putative dihydroxyacetone‑specific PTS enzymes: HPr component/EI component |
| Reads supporting (aligned to +/- strand): ref base C (385/138); new base T (33/8); total (420/146) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.59e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01 | |||||||||||
Only 100 of 613 total aligned reads displayed.
TGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCTTTCGCTGTTAACGTCATCAGATC > CP009273/1243555‑1243688 | tGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGAcc < 1:16741891/68‑1 (MQ=255) gTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGATCa > 1:29741999/1‑68 (MQ=255) gTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCa > 1:8105185/1‑68 (MQ=255) gTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCa > 1:2877579/1‑68 (MQ=255) gTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCa > 1:26445471/1‑68 (MQ=255) gTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCa > 1:31861282/1‑68 (MQ=255) gTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCa > 1:28257321/1‑68 (MQ=255) gTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCa > 1:18835084/1‑68 (MQ=255) ttCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAg > 1:6323682/1‑68 (MQ=255) tCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAg < 1:3218847/67‑1 (MQ=255) cTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGa > 1:7918600/1‑68 (MQ=255) tGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGa < 1:8690048/67‑1 (MQ=255) ggAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCg > 1:5803187/1‑68 (MQ=255) aGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGATCAGAAAAGATTGCg < 1:4627753/66‑1 (MQ=255) aGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGc > 1:2460573/1‑68 (MQ=255) aGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGc > 1:15344883/1‑68 (MQ=255) tCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCa < 1:26777957/66‑1 (MQ=255) tCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAat < 1:7066148/68‑1 (MQ=255) gCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGAGCAGAAAAGATTGCGGCAata > 1:12643847/1‑67 (MQ=255) cTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATc > 1:21016250/1‑68 (MQ=255) ttGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCg < 1:19645224/68‑1 (MQ=255) ttGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCg < 1:27801457/68‑1 (MQ=255) cgccgccAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTTCGGCAATATAGTCaa > 1:547033/1‑68 (MQ=255) cgccgccAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCaa > 1:13161306/1‑68 (MQ=255) gccAGCAGTTCCGGATCATCTAACAGTGTATGGTGATCAGAAAAGATTGCGGCAATATCGTCAAGccc < 1:16452433/68‑1 (MQ=255) gcagTTCCGGATCATCTAACAGTGTATGGTGATCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTg > 1:10014688/1‑68 (MQ=255) tCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCt < 1:19020553/66‑1 (MQ=255) tCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCt < 1:20547967/66‑1 (MQ=255) ggATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTga > 1:11351466/1‑67 (MQ=255) atcatcTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATGTCGTCGGGCCCGCTGGCTTCTGCtt > 1:23245342/1‑68 (MQ=255) atcatcTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCtt > 1:18642925/1‑68 (MQ=255) atcatcTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCtt > 1:2140047/1‑68 (MQ=255) atcatcTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCtt > 1:19332466/1‑68 (MQ=255) atcatcTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCtt > 1:4944796/1‑68 (MQ=255) atcatcTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCtt > 1:11865111/1‑68 (MQ=255) atcatcTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGAtt > 1:24182178/1‑68 (MQ=255) cTAACAGTGTATGGTGATCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCTTTCGCt < 1:29032531/68‑1 (MQ=255) cTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCTTTCGCt < 1:13332319/68‑1 (MQ=255) cTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCTTTCGCt < 1:21750563/68‑1 (MQ=255) aaCACTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCTTTCGCTGt < 1:22679771/68‑1 (MQ=255) aCAGTGTATGGTGATCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCTTTCGCTGtt > 1:27028442/1‑68 (MQ=255) aCAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGGTCGCTTCTGCTAACGCAGtt > 1:31293307/1‑68 (MQ=255) aCAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCTTTCGCTGtt > 1:19822367/1‑68 (MQ=255) aCAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCTTTCGCTGtt > 1:13927357/1‑68 (MQ=255) aCAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCTTTCGCTGtt > 1:21962333/1‑68 (MQ=255) aCAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCTTTCGCTGtt > 1:3339998/1‑68 (MQ=255) aCAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGATTTCGCTGtt > 1:14374346/1‑68 (MQ=255) aCAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAACCCCCCTCGCTTCTCCTTTAGCTggt > 1:9671283/1‑66 (MQ=255) cAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCTTTCGCTGTTa > 1:17258803/1‑68 (MQ=255) cAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCTTTCGCTGTTa > 1:1757276/1‑68 (MQ=255) aGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCTTTCGCTGttt > 1:4402883/1‑66 (MQ=255) aGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCTTTCGCTGTTa > 1:12832446/1‑67 (MQ=255) aGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCTTTCGCTGTTa > 1:28152850/1‑67 (MQ=255) aGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCTTTCGCTGTTa > 1:18161725/1‑67 (MQ=255) aGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCTTTCGCTGTTa > 1:2030909/1‑67 (MQ=255) aGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCTTTCGCTGTTa > 1:9076258/1‑67 (MQ=255) aGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCTTTCGCTGTTa > 1:9357275/1‑67 (MQ=255) aGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCTTTCGCTGTTa > 1:24900231/1‑67 (MQ=255) aGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCAGCTTTCGCTGTTa > 1:10906293/1‑67 (MQ=255) aGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTATGCTTTCGATGTTa > 1:5252321/1‑67 (MQ=255) aGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCACGCTGGCTTCTGCATTAGCTCTTaa > 1:17091053/1‑68 (MQ=255) aGTGTATGGTGACCAGAAAAGATTGCGGCAATATAGTCAAGCCCGCTGGGTTCTGCTTTCGCTGTTa > 1:20147146/1‑67 (MQ=255) gtATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCTTTCGCTGTTaa < 1:24316087/65‑1 (MQ=255) gtATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCAGCTGGCTTCTGCTTTCGCTGTTAACGt > 1:6026044/1‑68 (MQ=255) aTGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCTTTCGCTGTTAACGtca > 1:3705989/1‑68 (MQ=255) aTGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCTTTCGCTGTTAACGtca < 1:11733829/68‑1 (MQ=255) tGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCTTTCGCTGTTAACGtca > 1:23591572/1‑67 (MQ=255) tGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCTTTCGCTGTTAACGtca > 1:31582240/1‑67 (MQ=255) tGGTGACAAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCTTACGCTCTTAACGtca > 1:22166497/1‑67 (MQ=255) ggTGACCAGAAAAGATTGCGGCAATATTGTCAAGCCCGCTGGCTTCTGCTTTCGCTGTTAACGtcatc > 1:30922704/1‑68 (MQ=255) ggTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCTTTCGCTGTTAACGtgatc > 1:15758618/1‑68 (MQ=255) ggTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCTTTCGCTGTTAACGtcatc > 1:9244166/1‑68 (MQ=255) ggTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCTTTCGCTGTTAACGtcatc > 1:8379846/1‑68 (MQ=255) gTGATCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCTTTCGCTGTTAACGtcatca > 1:16106070/1‑68 (MQ=255) gTGATCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCTTTCGCTGTTAACGtcatca > 1:6722414/1‑68 (MQ=255) gTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCTTTCGCTGTTAACGtcatca > 1:13657867/1‑68 (MQ=255) gTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCTTTCGCTGTTAACGtcatca > 1:14247553/1‑68 (MQ=255) gTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCTTTCGCTGTTAACGtcatca > 1:23715043/1‑68 (MQ=255) gTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCTTTCGCTGTTAACGtcatca > 1:30400340/1‑68 (MQ=255) gTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCTTTCGCTGTTAACGtcatca > 1:12117853/1‑68 (MQ=255) gTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCTTTCGCTGTTAACGtcatca > 1:14728745/1‑68 (MQ=255) gTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCTTTCGCTGTTAACGtcatca > 1:30724103/1‑68 (MQ=255) gTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCTTTCGCTGTTAACGtcatca > 1:27953575/1‑68 (MQ=255) gTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCTTTCGCTGTTAACGtcatca > 1:27533746/1‑68 (MQ=255) gTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCAGATGGCTTATAATATCGATGTTAACGtcttca > 1:10154057/1‑68 (MQ=255) gACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCTTTCGCTGTTAACGTCATCAGa < 1:12014313/68‑1 (MQ=255) gACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCTTTCGCTGTTAACGTCATCAGa < 1:29422236/68‑1 (MQ=255) gACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCTTTCGCTGTTAACGTCATCAGa > 1:7664836/1‑68 (MQ=255) gACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCTTTCGCTGTTAACGTCATCAGa > 1:27337524/1‑68 (MQ=255) gACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCTTTCGCTGTTAACGTCATCAGa < 1:10398428/68‑1 (MQ=255) gACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCTTTCGCTGTTAACGTCATCAGa < 1:26024317/68‑1 (MQ=255) gACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCTTTCGCTGTTAACGTCATCAGa < 1:25263545/68‑1 (MQ=255) ccAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGGTTCTGCTTTCGCTGTTAACGTCATCAGa > 1:15012408/1‑66 (MQ=255) ccAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCTTTCGCTGTTAACGTCATCAGAt < 1:31093305/67‑1 (MQ=255) ccAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCTTTCGCTGTTAACGTCATCAGAt < 1:7447575/67‑1 (MQ=255) ccAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCTTTCGCTGTTAACGTCATCAGAt < 1:10773216/67‑1 (MQ=255) ccAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCTTTCGCTGTTAACGTCATCAGAt < 1:20811898/67‑1 (MQ=255) ccAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCTTTCGCTGTTAACGTCATCAGAt < 1:25665292/67‑1 (MQ=255) ccAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCTTTCGCTGTTAACGTCATCAGATc < 1:24006504/68‑1 (MQ=255) ccAGAAAAGATTGCGGCAATATCGGCAAGCCCGCTGGCTTCTGCTTTCGCTGTTAACGTCATCAGAt < 1:14630741/67‑1 (MQ=255) | TGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCTTTCGCTGTTAACGTCATCAGATC > CP009273/1243555‑1243688 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |