| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,187,952 | A→G | 6.2% | intergenic (+68/+130) | yqiK → / ← sibD | PHB family membrane protein, function unknown/sRNA antisense regulator of toxic IbsD protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,187,952 | 0 | A | G | 6.2% | 1233.6 / 41.7 | 393 | intergenic (+68/+130) | yqiK/sibD | PHB family membrane protein, function unknown/sRNA antisense regulator of toxic IbsD protein |
| Reads supporting (aligned to +/- strand): ref base A (217/146); new base G (5/19); total (228/165) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.15e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01 | |||||||||||
Only 100 of 451 total aligned reads displayed.
ACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGA > CP009273/3187885‑3188019 | cccTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAg > 1:15368315/1‑68 (MQ=255) ccTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGt < 1:27720902/68‑1 (MQ=255) ccTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGt < 1:517319/68‑1 (MQ=255) ctctTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTg < 1:11651247/68‑1 (MQ=255) ctctTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGt > 1:2963315/1‑67 (MQ=255) ctctTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGt > 1:21970728/1‑67 (MQ=255) ctctTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGt > 1:18552992/1‑67 (MQ=255) ctTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCa < 1:18002034/68‑1 (MQ=255) tCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCaaa > 1:25007184/1‑68 (MQ=255) ccATGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAAt < 1:26010385/68‑1 (MQ=255) ccAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAAt > 1:8524564/1‑68 (MQ=255) cAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATg < 1:3611090/68‑1 (MQ=255) cAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATg < 1:11485423/68‑1 (MQ=255) cAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATg < 1:21470393/68‑1 (MQ=255) cAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATg < 1:7513059/68‑1 (MQ=255) atgAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATgc < 1:9629304/66‑2 (MQ=255) acgAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATgc < 1:10973070/66‑2 (MQ=255) aGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATgc < 1:1065594/68‑2 (MQ=255) gAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATc > 1:11823488/1‑68 (MQ=255) gAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATc > 1:20328931/1‑68 (MQ=255) gAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATc > 1:19676723/1‑68 (MQ=255) aataatCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAATGTGCAAATGATCa > 1:14373922/1‑68 (MQ=255) aataatCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATc < 1:18382304/67‑1 (MQ=255) aataatCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCa > 1:8359729/1‑68 (MQ=255) ataatCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAg > 1:27987042/1‑68 (MQ=255) ataatCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAg > 1:2729443/1‑68 (MQ=255) ataatCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAg > 1:30159391/1‑68 (MQ=255) tCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGgttg > 1:9083665/1‑68 (MQ=255) tCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGgtag > 1:26697044/1‑66 (MQ=255) cccTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAGGTGCAAATGATCAggtggt > 1:29047491/1‑68 (MQ=255) cccTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAggtggt > 1:25682004/1‑68 (MQ=255) cccTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGgttgt > 1:30671536/1‑68 (MQ=255) cccTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGgttgt > 1:17470608/1‑68 (MQ=255) cccTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGgttgt > 1:24723756/1‑68 (MQ=255) cccTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGgttgt > 1:27043626/1‑68 (MQ=255) cccTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGgatgt > 1:426856/1‑68 (MQ=255) ccTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGgttgtt < 1:31616603/68‑1 (MQ=255) ccTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGgttgtt < 1:17006366/68‑1 (MQ=255) tGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAATGTGCAAATGATCAGGATGTTgc > 1:31482860/1‑68 (MQ=255) tGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTgc < 1:6199382/68‑1 (MQ=255) cccAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTgcagc > 1:29463875/1‑68 (MQ=255) ccAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTgcagca > 1:9220840/1‑68 (MQ=255) ccAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTgcagc < 1:21706625/67‑1 (MQ=255) cAAACAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCAt < 1:28273893/68‑1 (MQ=255) cAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCAt < 1:13212744/68‑1 (MQ=255) cAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCAt < 1:4717762/68‑1 (MQ=255) aaaGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCAtttt < 1:22419710/68‑1 (MQ=255) aaaGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCAtttt < 1:5441294/68‑1 (MQ=255) aaaGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCAtttt < 1:7821268/68‑1 (MQ=255) aaGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCAtttt > 1:5676168/1‑67 (MQ=255) aGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGc > 1:12547066/1‑68 (MQ=255) aGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGc > 1:31738941/1‑68 (MQ=255) gCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCg > 1:31959729/1‑68 (MQ=255) gCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCg > 1:1885854/1‑68 (MQ=255) ccGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGt > 1:22039107/1‑68 (MQ=255) aTTTTTTATTTCTGCCTTCCGCTATAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACgg < 1:17300800/67‑1 (MQ=255) aTTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCCGGTTGTTGCAGCATTTTGCGTGACgg < 1:31893902/67‑1 (MQ=255) ttttttATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGc < 1:4877047/67‑1 (MQ=255) ttttttATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGc < 1:128221/67‑1 (MQ=255) ttttttATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGc < 1:22309013/67‑1 (MQ=255) ttttttATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGc < 1:31245933/67‑1 (MQ=255) ttttttATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGc < 1:3401928/67‑1 (MQ=255) ttttttATATCTCCGTTCCGCTAAAAAGTGCAAATGATCACGTTGTTGCAGCATTTTGCGTGACGGc < 1:29773726/67‑1 (MQ=18) ttttttATATCTCCGTTCCGCTAAAAAGGGCAAATGATCACGTTGGTGCAGCATTTTGCGTGACGGc < 1:26460876/67‑1 (MQ=18) ttATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCggg < 1:6821867/68‑1 (MQ=255) ttATATCTGCGTTCCGCAAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCggg < 1:19899857/68‑1 (MQ=255) tatCTGCGTTCCGCTAAAAAGTGCAAATGCTCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGaa > 1:15140703/1‑68 (MQ=255) tatCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGaa > 1:18987872/1‑68 (MQ=255) tatCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGaa > 1:15848124/1‑68 (MQ=255) atCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAg < 1:6633081/68‑1 (MQ=255) atCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAg < 1:19113858/68‑1 (MQ=255) atCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAg > 1:23672329/1‑68 (MQ=255) tCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGTCGGCTCGGGGAAg > 1:29274406/1‑67 (MQ=255) tCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAg > 1:21019827/1‑67 (MQ=255) tCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAg > 1:13474763/1‑67 (MQ=255) tCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAg > 1:23339695/1‑67 (MQ=255) tCTGCGTCCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAg > 1:987400/1‑67 (MQ=255) cTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGc < 1:30336287/68‑1 (MQ=255) gTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCaaa > 1:29758457/1‑67 (MQ=255) gTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCaaa < 1:23042805/67‑1 (MQ=255) tCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCaaaagt > 1:19191534/1‑66 (MQ=255) tCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCaaa > 1:13808799/1‑65 (MQ=255) tCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCaaa > 1:4926070/1‑65 (MQ=255) tCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCaaa > 1:24361285/1‑65 (MQ=255) cGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGc < 1:6369080/68‑1 (MQ=255) cGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGc < 1:17838256/68‑1 (MQ=255) cGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGc < 1:28603495/68‑1 (MQ=255) gCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCa > 1:19304034/1‑68 (MQ=255) aaaaGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCag < 1:14717422/68‑1 (MQ=255) aaaaGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGGGACGGCTCGGGGAAGGCAAAACTGCATCag < 1:30500321/68‑1 (MQ=255) aaaGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGCCGGCTCGGGGAAGGCAAAACTGAATCaga > 1:2078972/1‑68 (MQ=255) aaaGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAAGTGCATCaga > 1:10056310/1‑68 (MQ=255) aaaGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCaga > 1:22769178/1‑68 (MQ=255) aaaGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCaga > 1:7300017/1‑68 (MQ=255) aaaGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCaga > 1:2403184/1‑68 (MQ=255) aaaGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCaga > 1:25828463/1‑68 (MQ=255) aaaGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCaga < 1:16283504/68‑1 (MQ=255) aaaGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCaga > 1:16056900/1‑68 (MQ=255) aaGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCagag < 1:5920697/68‑1 (MQ=255) aGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAgaga > 1:10345155/1‑68 (MQ=255) | ACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGA > CP009273/3187885‑3188019 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |