| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,462,400 | A→G | 7.8% | S271S (AGT→AGC) | chiA ← | periplasmic endochitinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,462,400 | 0 | A | G | 7.8% | 1212.2 / 35.6 | 402 | S271S (AGT→AGC) | chiA | periplasmic endochitinase |
| Reads supporting (aligned to +/- strand): ref base A (187/172); new base G (24/7); total (223/179) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.86e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 408 total aligned reads displayed.
AGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAA > CP009273/3462336‑3462467 | aGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTg > 1:22788988/1‑68 (MQ=255)aGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTg > 1:21227875/1‑68 (MQ=255)aGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTg > 1:29361632/1‑68 (MQ=255)aGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTg > 1:30009657/1‑68 (MQ=255)aGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTg > 1:3174582/1‑68 (MQ=255)aGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTg > 1:5917441/1‑68 (MQ=255)aGCCAGGGTTACCATACCGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTg > 1:9641017/1‑68 (MQ=255) ccAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTGCTGGc > 1:1712292/1‑68 (MQ=255) ccAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGc > 1:31581787/1‑68 (MQ=255) cAGGTTTACCATACTGTCTGATTTCTGTTGCCGTAGCCGCTCGAACATAACGCCATGGGTTGCTGGCa < 1:5074635/68‑1 (MQ=17) cAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTgg < 1:15877037/66‑1 (MQ=255) cAGGGTTACCATACTGGCTGATTTCAGTTGCCGTTGCGGCTCGAACATAACGCCATGGGTTGCTGGCa < 1:11280967/68‑1 (MQ=18) ggTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTTCTGGCATca > 1:3327538/1‑67 (MQ=255) ggTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCg > 1:1184520/1‑68 (MQ=255) ggTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCg > 1:28975329/1‑68 (MQ=255) ttACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATTACGCCATGGGTTACTGGCATCGt < 1:13210846/67‑1 (MQ=255) ttACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGt < 1:15087363/67‑1 (MQ=255) ttACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGt < 1:17553505/67‑1 (MQ=255) gtACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACAAAACGCCATGGGTTACTGGCATCGt < 1:10524381/66‑1 (MQ=255) aCCATACTGGCTGATTTCTGTTGCCGTTGCCGCTCGAACATAACGCCATGGGTTGCTGGCATCAttgc < 1:21809715/68‑3 (MQ=17) cATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTGCTGGCATCCtttgtt > 1:19327660/1‑65 (MQ=21) cATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGttttgt > 1:13478746/1‑66 (MQ=255) cATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttct > 1:1464591/1‑68 (MQ=255) cATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttct < 1:22013378/68‑1 (MQ=255) cATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttct > 1:678305/1‑68 (MQ=255) cATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtctct < 1:23128599/68‑1 (MQ=255) cATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCAtttgtt > 1:11387743/1‑65 (MQ=255) cATACTGGCTGATTTCTGATGCCGTTGCCGCGCGAACCTAACGCCATGGGTTACTGGCATCGTtttct < 1:14222550/68‑1 (MQ=37) aTACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttctt > 1:31255346/1‑68 (MQ=255) aTACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttctt > 1:12476176/1‑68 (MQ=255) aTACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttctt > 1:4012290/1‑68 (MQ=255) tACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTTCTGGCATCGTtttctt > 1:21403137/1‑67 (MQ=255) tACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTTCTGGCATCCTttgctt > 1:4313396/1‑67 (MQ=21) tACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttctt > 1:31407006/1‑67 (MQ=255) tACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttctt > 1:3463077/1‑67 (MQ=255) aCTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttgtttc > 1:27168305/1‑68 (MQ=255) aCTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttctttc > 1:19451278/1‑68 (MQ=255) aCTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCATttaatttc > 1:27299831/1‑68 (MQ=21) cTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTttggtttc > 1:30572636/1‑67 (MQ=255) tGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTGCTGGCATCATTGGTTTTCGc > 1:20456958/1‑68 (MQ=14) tGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGc > 1:9263652/1‑68 (MQ=255) ggCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCa > 1:8878729/1‑68 (MQ=11) ttaTTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCg < 1:27658284/66‑1 (MQ=255) tGATTTCTGTTGCCGTTGCCGCTCGAACTTAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCg < 1:20019288/68‑1 (MQ=255) tGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTGCTGGCATCGTTTTGTTTCGCATc > 1:24407769/1‑67 (MQ=255) tGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCg < 1:20799781/68‑1 (MQ=255) tGATTTCTGCTGCGGTTGCGGCTCGAACATTACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCg < 1:27433241/68‑1 (MQ=255) ttCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTGTGTTTCGCATCGccag > 1:4776237/1‑66 (MQ=21) tCTGTTGCCGTTGCCGCGCGAGCATAACGCCATGGGTTGCTGGCATCATTGGTTTTCGCATCGCCAgg > 1:8382203/1‑68 (MQ=11) tGTTGCCGTTGCCTCGCGAACATAACGCCATGGGTTACTGTCATCGTTTTCTTTCGCATCGCCTGGGc < 1:8569034/68‑1 (MQ=37) tGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGc < 1:28015909/68‑1 (MQ=255) gttgccgttgccGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCa > 1:10223606/1‑68 (MQ=255) gttgccgttgccGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCa > 1:9431089/1‑68 (MQ=255) cgttgccgCGCGAGCATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAAttt > 1:23271171/1‑67 (MQ=255) cgttgccgCGCGAACATAACGCCATGGGTTGCTgg > 1:14000028/1‑35 (MQ=255) cgttgccgCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAAttt > 1:18915599/1‑67 (MQ=255) cgttgccgCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAAttt > 1:28198722/1‑67 (MQ=255) cgttgccgCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTg > 1:22304377/1‑68 (MQ=255) gccgCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCcgc < 1:26302370/68‑1 (MQ=255) gccgCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCcgc < 1:17714266/68‑1 (MQ=255) cgcgcgAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCcgcg < 1:14944314/67‑1 (MQ=255) gcgcgAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGcc < 1:16560393/68‑1 (MQ=255) gcgcgAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGcc < 1:29401250/68‑1 (MQ=255) gcgAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCt < 1:25376289/67‑1 (MQ=255) gcgAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTa < 1:12851194/68‑1 (MQ=255) gAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTAcc < 1:24827767/68‑1 (MQ=255) aCATCACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACcca < 1:26943726/68‑1 (MQ=255) aCATCACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACcca < 1:7192780/68‑1 (MQ=255) aCATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTAccc < 1:7909694/67‑1 (MQ=255) tAACGCCATGGGTTAGTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACccacca < 1:11526152/68‑1 (MQ=255) tAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACccacca < 1:30848617/68‑1 (MQ=255) cGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGc < 1:23489715/68‑1 (MQ=255) cGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGc > 1:5623959/1‑68 (MQ=255) cGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCAGGGCAATTTGCCGCGCCTACCCACCAGGc > 1:76517/1‑68 (MQ=255) gCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCg > 1:22553164/1‑68 (MQ=255) gCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGAAGCTCCTACCCACCAGGca > 1:7489521/1‑67 (MQ=25) ccATGGGTTACTGGCATCGTTTTGTTTCGCATCCCCAGGGCAGTTTGAAGAGCCTACCCACCAGGcat > 1:19146409/1‑66 (MQ=14) ccATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGt > 1:13652218/1‑68 (MQ=255) ccATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGt > 1:4635902/1‑68 (MQ=255) ccATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCAGGTCAATTAGCAGCGCCTACCCACCAGGcat > 1:25111040/1‑66 (MQ=21) ccATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCAGGGCAGTTTGCCGCGCCTACCCACCAGGcat > 1:15600542/1‑66 (MQ=37) cATGGGTTACTGGCTTCTTTTTCTTTCGCATCGCCTGGGCAATTTTCCGCGCCTACCCACCAGGCGt < 1:25984931/67‑1 (MQ=255) cATGGGTTACTGGCCTCGTTTGCTCTGGCATCGCCTGGGCAATGTGCCGCGCCTACCCACCAGGCGtt < 1:32049237/68‑1 (MQ=255) cATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGtt < 1:2883326/68‑1 (MQ=255) cATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGtt < 1:10001066/68‑1 (MQ=255) cATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGt < 1:24520544/67‑1 (MQ=255) cATGGGTTACTGGCATCGTGTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGtt < 1:20291009/68‑1 (MQ=255) tGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCt > 1:21877675/1‑68 (MQ=255) tGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCt < 1:18136577/68‑1 (MQ=255) tGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCt < 1:2391856/68‑1 (MQ=255) tGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCt < 1:18655026/68‑1 (MQ=255) tGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCt < 1:2986292/68‑1 (MQ=255) gggTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCtt > 1:29554973/1‑68 (MQ=255) ggTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTta > 1:18507235/1‑68 (MQ=255) ggTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGACGCGCCTACCCACCAGGCTTTTTta > 1:8312958/1‑68 (MQ=37) ggTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGACGCGCCTACCCACCAGGCTTTTTta > 1:8518406/1‑68 (MQ=37) ggTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTAGCCGCGCCTACCCACCAGGCGTTTTta > 1:11655798/1‑68 (MQ=255) ggTTACTGGCATCGTTTTCTTTCGCATCGCCGGGGCAGTTAGCCGCGGCTACCCACCAGGCGTTTTta > 1:12039295/1‑68 (MQ=21) ggTTACTGGCATCGTTTTCTTTCGCATCGCCAGGGCAGTTTGAAGAGCCTACCCACCAGGCATTTTta > 1:10952277/1‑68 (MQ=14) aCTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCATTTTtata > 1:15326780/1‑66 (MQ=255) | AGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAA > CP009273/3462336‑3462467 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |