| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,462,439 | C→A | 7.9% | A258A (GCG→GCT) ‡ | chiA ← | periplasmic endochitinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,462,439 | 0 | C | A | 7.9% | 1892.1 / 47.4 | 618 | A258A (GCG→GCT) ‡ | chiA | periplasmic endochitinase |
| Reads supporting (aligned to +/- strand): ref base C (129/432); new base A (25/31); total (154/464) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.61e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 663 total aligned reads displayed.
GCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTT > CP009273/3462374‑3462506 | gccgCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCcgc < 1:26302370/68‑1 (MQ=255)gccgCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCcgc < 1:17714266/68‑1 (MQ=255) ccgCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCcgc > 1:2799056/1‑67 (MQ=255) gcgcgAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGcc < 1:1399468/68‑1 (MQ=255) gcgAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTa < 1:12851194/68‑1 (MQ=255) gAACTTTACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTAcc < 1:31995527/68‑1 (MQ=255) aCATCACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACcca < 1:7192780/68‑1 (MQ=255) cGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGAAGAGGCTACCCACCAGGc > 1:13271445/1‑68 (MQ=18) gCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCAGCGCCTACCCACCAGGCg > 1:17330302/1‑68 (MQ=255) ccATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGCCATTTAGCAGCGCCTACCCACCAGGCGt > 1:11665102/1‑68 (MQ=25) cATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGtt < 1:10001066/68‑1 (MQ=255) cATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGt < 1:24520544/67‑1 (MQ=255) tGGGTTATTGGATTCTTGTTCTTTGGCATCGCCTGGGCAATTTTCAGCGCCTACCCACCAGGCGTTCt < 1:3322080/68‑1 (MQ=21) tGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCt < 1:18136577/68‑1 (MQ=255) tGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCt < 1:2887900/68‑1 (MQ=255) tGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCt < 1:21830079/68‑1 (MQ=255) tGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCt < 1:15054183/68‑1 (MQ=255) ggTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGACGCGCCTACCCACCAGGCTTTTTta > 1:8518406/1‑68 (MQ=37) aCTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATaaa > 1:19899486/1‑68 (MQ=255) tGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTTTGATAAAtt > 1:5926361/1‑67 (MQ=39) tGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCATTTTtatataa > 1:15453224/1‑64 (MQ=37) tGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCATTATTATaaaa > 1:24082184/1‑66 (MQ=38) tGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGAAGAGCCTACCCACCAGGCGTTTTAATAAAtt > 1:11819109/1‑67 (MQ=21) ggCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATaaa > 1:27149646/1‑65 (MQ=255) ggCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGATCTTATAAATg > 1:21013209/1‑67 (MQ=255) gCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGAGCCTACCCACCACGCGTTCATATAAATgcc > 1:18783507/1‑68 (MQ=255) tCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACTAGGCGTTCTTATAAATgccgcc < 1:22207824/68‑1 (MQ=255) tCGATTCCTTTCGGATCGCCTGGGCAATGTTCAGAGCCTACCCACCAGGCGTTCTTATAAATgccgcc < 1:8202742/68‑1 (MQ=21) cGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGccgccg < 1:18452805/68‑1 (MQ=255) tttttCTTTCGCATCGCCTGGGCAATTTTCCGCGGCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:14237339/67‑1 (MQ=39) ttcttctatcGTATCGCCTGGGCAATTTGACGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:23092128/65‑1 (MQ=38) gTTTTCTTTGGGATCGCGTGGGCAATTTGCCGAGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:24780082/68‑1 (MQ=37) gTTTTCTTTGGCATCGCCTGGGCAATTTTCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:10852835/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:21485225/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:25222593/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:11164802/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:22961996/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:3088967/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:9425219/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:3533883/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:15740988/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:7572181/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:6572651/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:12635388/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:20153600/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:19573816/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:4688621/68‑1 (MQ=255) ttttCTTTGGCAGCGCCTGGGCAATTTTTAGAGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:24936969/67‑1 (MQ=21) ttttCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGtt > 1:29618352/1‑68 (MQ=255) ttttCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGtt > 1:29890189/1‑68 (MQ=255) tttctttgGGATCGCCTGGGCAATTTTACGCGCCAACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:933380/66‑1 (MQ=25) tttctttcGCATCGCCTGGTCAATTTTCCGCGCGTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:26091954/66‑1 (MQ=39) tttctttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGtt < 1:25483978/67‑1 (MQ=255) ttctttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTtat < 1:23483640/68‑1 (MQ=255) ctttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAg < 1:6112715/68‑1 (MQ=255) ttcGCATCGCCTGTGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:23910951/68‑1 (MQ=255) ttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:12192063/68‑1 (MQ=255) ttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:13098933/68‑1 (MQ=255) ttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCCTTCTTATAAATGCCGCCGTTAAAGAt > 1:575415/1‑68 (MQ=255) ctggCACCGCGTGGGCAATCTTTCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:9088349/65‑1 (MQ=18) tcGCATCGCCTGGGCAATATTACGAGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATa < 1:8824147/68‑1 (MQ=37) taTCGCGTGGGCAATCTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAAc < 1:28350699/66‑1 (MQ=38) ctCGCCTTGTCATTTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGt < 1:9613129/67‑1 (MQ=38) aTCGCCTGGGCAATTTGCCGGGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGt < 1:7390712/68‑1 (MQ=255) aTCGCCTGGGCAATTTGCCGCGCCTACCCAGCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGt < 1:26928433/68‑1 (MQ=255) aTCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGt < 1:27591952/68‑1 (MQ=255) aTCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGt < 1:3125241/68‑1 (MQ=255) aTCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGt < 1:15232692/68‑1 (MQ=255) aTCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGt < 1:6965303/68‑1 (MQ=255) aTCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGt < 1:16650429/68‑1 (MQ=255) aTCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGt < 1:4558896/68‑1 (MQ=255) aTCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTGATAAATGCCGCCGTTATAGATAACGt > 1:2044912/1‑68 (MQ=255) gcgtGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTAcc < 1:11992727/65‑1 (MQ=255) gCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTAcc < 1:20756166/68‑1 (MQ=255) ccTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCa > 1:5396787/1‑68 (MQ=255) ccTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTATTATAAATCCCGCCGTTATAGAAAACGTACCa > 1:5625465/1‑68 (MQ=255) cTGGGCAATTTTAAGAGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:31767942/68‑1 (MQ=37) cTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAATTGCCGCCGTTATAGATAACGTACCAt < 1:17559209/68‑1 (MQ=255) cTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:1150743/68‑1 (MQ=255) cTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:21142542/68‑1 (MQ=255) cTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:25924053/68‑1 (MQ=255) cTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:26705087/68‑1 (MQ=255) cTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:30794016/68‑1 (MQ=255) cTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:10220884/68‑1 (MQ=255) ggCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTa > 1:21305945/1‑68 (MQ=255) gCAATTTTCCGAGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTa < 1:14486213/67‑1 (MQ=255) gCAATTTTCAGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTa < 1:28514339/67‑1 (MQ=255) gCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTa < 1:4972912/67‑1 (MQ=255) gCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTa < 1:13481593/67‑1 (MQ=255) cAATTTGCCGCGCCTACCGACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTag < 1:19172051/67‑1 (MQ=255) cAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATATCGTACCATGTaca > 1:29339284/1‑66 (MQ=255) cAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTaga > 1:4078131/1‑68 (MQ=255) cAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTag < 1:16031364/67‑1 (MQ=255) ttGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTagaaga > 1:10560726/1‑67 (MQ=255) ttGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCAGCCGTTTTAGATAACGTGCGATGTataagc > 1:7861269/1‑66 (MQ=37) tGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAgaagaa > 1:31539120/1‑67 (MQ=255) cgcgCCTACCCACCGGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCtt < 1:29119453/68‑1 (MQ=255) cgcgCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCtt < 1:5271058/68‑1 (MQ=255) cgcgCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCt > 1:19050585/1‑67 (MQ=255) cgcgCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCt < 1:30167656/67‑1 (MQ=255) | GCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTT > CP009273/3462374‑3462506 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |