| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,462,676 | A→T | 6.5% | A179A (GCT→GCA) | chiA ← | periplasmic endochitinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,462,676 | 0 | A | T | 6.5% | 2284.3 / 34.8 | 674 | A179A (GCT→GCA) | chiA | periplasmic endochitinase |
| Reads supporting (aligned to +/- strand): ref base A (360/258); new base T (7/49); total (367/307) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.73e-11 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 678 total aligned reads displayed.
CCGGTGAAGGCTGTGGTGCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAGTTAGA > CP009273/3462609‑3462741 | ccGGTGAAGGCTGTGGTGCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTa > 1:6776308/1‑68 (MQ=255) tGAAGGCTGTGGTGCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCg > 1:16665600/1‑67 (MQ=255) gAAGGCTGTGGTGCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCTGTAGCgg < 1:11961240/67‑1 (MQ=255) gAAGGCTGGGGTGCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGc < 1:1752145/68‑1 (MQ=255) aaGGCTGTGGCGCTGAAGTACAGGACTGTCGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGc < 1:23307169/67‑1 (MQ=255) aGGCTGTGGTGCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCa < 1:20337220/67‑1 (MQ=255) aGGCTGTGGTGCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCAc > 1:171897/1‑68 (MQ=255) aGGCTGGGGGGCTGAAGTACAGGACTGAGTATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCa < 1:8051187/67‑1 (MQ=255) gCTGTGGTGCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGa > 1:24290195/1‑68 (MQ=255) gCTGTGGTGCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGa > 1:11269914/1‑68 (MQ=255) gCTGTGGTGCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGa > 1:7515399/1‑68 (MQ=255) cTGTGGTGCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGa < 1:10021278/67‑1 (MQ=255) cTGTGGTGCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGa < 1:28256253/67‑1 (MQ=255) cTGTGGTGCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGa < 1:16462360/67‑1 (MQ=255) cTGTGGTGCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGa > 1:29884080/1‑67 (MQ=255) tgtgGTGCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAAc < 1:19002044/68‑1 (MQ=255) tgtgGTGCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAAc < 1:4541218/68‑1 (MQ=255) tgCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAAc > 1:27366179/1‑68 (MQ=255) gCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACg < 1:19719696/68‑1 (MQ=255) tGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGcc > 1:19271456/1‑68 (MQ=255) tGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGc < 1:14530907/67‑1 (MQ=255) aGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATg > 1:25688354/1‑68 (MQ=255) aGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATg > 1:27862208/1‑68 (MQ=255) aGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATg > 1:5144885/1‑68 (MQ=255) aCAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATgg < 1:14009650/66‑1 (MQ=255) aGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGtt > 1:2972219/1‑67 (MQ=255) aGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGtt > 1:18677517/1‑67 (MQ=255) aGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGtt > 1:16257652/1‑67 (MQ=255) aGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGtt > 1:3245297/1‑67 (MQ=255) aGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGtt > 1:6553575/1‑67 (MQ=255) ggACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGtt > 1:11457545/1‑66 (MQ=255) gACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGtt > 1:21322351/1‑65 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATgg > 1:22133813/1‑61 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGtt < 1:29553553/64‑1 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGGTg > 1:24651403/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:267752/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:9441165/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:9827972/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:2813529/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:27585527/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:29072896/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:31590676/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:29259806/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:31375064/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:30392451/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:30621688/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:12783598/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:11016072/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:22286315/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:12968690/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:21525221/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:21000198/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:22838844/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCAAGGGTTGGTg > 1:15327715/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCAGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:18401842/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATATCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:25104138/1‑68 (MQ=255) aCTGTGGATTACTGGTTCCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:18085793/1‑68 (MQ=255) gtgGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGca < 1:28651895/68‑1 (MQ=255) gtgGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGca > 1:15231735/1‑68 (MQ=255) ggATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGcatc > 1:16904901/1‑68 (MQ=255) ggATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGcatc > 1:25239945/1‑68 (MQ=255) aTTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGcatcat > 1:14311517/1‑68 (MQ=255) aTTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGcatcat > 1:7022799/1‑68 (MQ=255) aTTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGcatcat > 1:22526895/1‑68 (MQ=255) ttACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCAtt < 1:12418171/68‑1 (MQ=255) aCTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCAtt > 1:30917706/1‑66 (MQ=255) aCTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCAtt > 1:31792741/1‑66 (MQ=255) aCTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCAtt > 1:7278669/1‑66 (MQ=255) aCTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCAtt > 1:1708701/1‑66 (MQ=255) aCTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCAtt > 1:5446539/1‑66 (MQ=255) tGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCtt > 1:27069080/1‑68 (MQ=255) tttCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTc > 1:8740515/1‑68 (MQ=255) tCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGc > 1:10637650/1‑68 (MQ=255) tgaTTTCTGTTGCCGTTGCGGCTCGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCa < 1:11839996/67‑1 (MQ=17) tgaTTTCTGTTGCCGTAGCCGCTCGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCa < 1:27190051/67‑1 (MQ=17) tgaTTTCAGTTGCCGTAGCCGCTCGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCa < 1:20003918/67‑1 (MQ=18) gaTTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCa < 1:3027951/67‑1 (MQ=255) gaTTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCa < 1:8406292/67‑1 (MQ=255) gaTTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCa > 1:25439127/1‑67 (MQ=255) gaTTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCa < 1:2171085/67‑1 (MQ=255) gaTTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCa < 1:3430708/67‑1 (MQ=255) tGCCGTTGCGGCTCGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAg < 1:6202068/67‑1 (MQ=255) tGCCGTTGCGGCTCGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAg < 1:13498674/67‑1 (MQ=255) tGCCGTTGCGGCGCGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAg < 1:15560546/67‑1 (MQ=255) tGCCGTTGCCGCTCGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAg < 1:17914672/67‑1 (MQ=18) tGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAg < 1:7817453/67‑1 (MQ=255) tGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAg < 1:10262607/67‑1 (MQ=255) tGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAg < 1:14853614/67‑1 (MQ=255) tGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAg < 1:13233389/67‑1 (MQ=255) tGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAg < 1:13762352/67‑1 (MQ=255) tGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAg < 1:16011482/67‑1 (MQ=255) tGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAg < 1:23833195/67‑1 (MQ=255) tGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAg < 1:23026015/67‑1 (MQ=255) tGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAGt < 1:3886969/68‑1 (MQ=255) tGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAGt < 1:26108588/68‑1 (MQ=255) tGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAGt < 1:9077389/68‑1 (MQ=255) gCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAGtt < 1:13619478/68‑1 (MQ=255) cGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCATTGATTTTCGCATCACCCGGCCAGTTag > 1:5979574/1‑68 (MQ=14) cGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAGTTag > 1:26526491/1‑68 (MQ=255) gTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAGTTaga < 1:4176474/68‑1 (MQ=255) | CCGGTGAAGGCTGTGGTGCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAGTTAGA > CP009273/3462609‑3462741 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |