| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 4,076,055 | T→G | 6.4% | Q22P (CAG→CCG) | fdoH ← | formate dehydrogenase‑O, Fe‑S subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 4,076,055 | 0 | T | G | 6.4% | 1264.1 / 12.1 | 517 | Q22P (CAG→CCG) | fdoH | formate dehydrogenase‑O, Fe‑S subunit |
| Reads supporting (aligned to +/- strand): ref base T (173/298); new base G (38/2); total (217/300) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.62e-14 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 520 total aligned reads displayed.
CTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATG > CP009273/4075990‑4076122 | tttACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTgcg > 1:14990967/1‑66 (MQ=255) tttACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAg < 1:28219858/68‑1 (MQ=255) aCAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGgc > 1:22200531/1‑67 (MQ=255) aCAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGgc > 1:29653967/1‑67 (MQ=255) aCAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGgc > 1:28647571/1‑67 (MQ=255) cAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGgcgc > 1:17821798/1‑68 (MQ=255) cAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGgcgc > 1:11810865/1‑68 (MQ=255) aGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGcgcg > 1:3500707/1‑68 (MQ=255) aGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGcgcg > 1:6856032/1‑68 (MQ=255) aGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGcgcg > 1:4775877/1‑68 (MQ=255) aGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCGGGGGcgcg > 1:2936335/1‑68 (MQ=255) gCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCgg > 1:4617316/1‑68 (MQ=255) gCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCGGAGGGGCgg > 1:12313673/1‑68 (MQ=255) gCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCCTCCTGGAAGTCCCGCGCCTGAGGCGCgg > 1:4941326/1‑68 (MQ=255) gCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTATTCCTGGAAGTCCCCCGCCAGAGGCGCgg > 1:9481562/1‑68 (MQ=37) ctaTACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGgcgc < 1:10565105/62‑1 (MQ=255) cGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCgggg < 1:18320826/68‑1 (MQ=255) cGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCgggg < 1:610643/68‑1 (MQ=255) cGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCgggg < 1:8984158/68‑1 (MQ=255) gATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGACGTCCCGCGCCTGAGGCGCggggg < 1:23384339/68‑1 (MQ=255) gATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCggggg < 1:18142607/68‑1 (MQ=255) gATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCggggg < 1:5991071/68‑1 (MQ=255) gATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCggggg < 1:2540665/68‑1 (MQ=255) tACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGt < 1:30972848/67‑1 (MQ=255) tACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGt < 1:25790738/67‑1 (MQ=255) tACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCCCGCCTGAGGCGCGGGGGTc > 1:30091051/1‑68 (MQ=255) aCAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCCCGCCTGAGGCGCGGGGGTCa > 1:15208917/1‑68 (MQ=255) cAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCCCGCCTGAGGCGCGGGGGTCAg > 1:26412290/1‑68 (MQ=255) ggtggtAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGa > 1:19916296/1‑67 (MQ=255) ggtggtAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCCCGCCTGTGCCGCGGGGGTCata > 1:5488244/1‑65 (MQ=255) ggtggtAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCCCGCCAGAGGCGCCGGGGTCCGa > 1:19273975/1‑67 (MQ=255) gtggtAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGAcc > 1:27005629/1‑68 (MQ=255) tggtAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGa < 1:25617279/65‑1 (MQ=255) tggtAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGa < 1:919484/65‑1 (MQ=255) tggtAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGa < 1:15844991/65‑1 (MQ=255) tggtAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGCCCg > 1:25959180/1‑68 (MQ=255) tggtAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGAc < 1:7905014/66‑1 (MQ=255) tggtAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCGGAGGCGCGGGGGTCAGACCg > 1:26220796/1‑68 (MQ=255) tggtAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCCCGCGGGAGGCGCGGGGGTCAGCCCg > 1:1669358/1‑68 (MQ=255) tcgtAACGTCGACTAGTTTCGACACTTCTACCTGGACGGCCCGCGCCTGAGGCGCGGGGGTCAGa < 1:21959197/63‑1 (MQ=255) gggtAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCGGAGGCGCGGGGGTCAGa < 1:11558516/64‑1 (MQ=255) ggtAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGt < 1:17367045/68‑1 (MQ=255) gtAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCGGAGGCGCGGGGGTCGGACCGtt > 1:10786007/1‑68 (MQ=255) tAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGGCAGCCCGttt > 1:2362847/1‑67 (MQ=255) tAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCGGAGGCGCGGGGGTCAGCCCGTTa > 1:24151786/1‑68 (MQ=255) aCGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAg > 1:10027476/1‑67 (MQ=255) aCGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAg > 1:17125987/1‑67 (MQ=255) aCGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAg > 1:1969521/1‑67 (MQ=255) aCGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAg > 1:3241706/1‑67 (MQ=255) aCGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCGGAGGCGCGGGGGTCAGCCCGTTAg > 1:20273056/1‑67 (MQ=255) cGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGGCAGACAGTTAGTc > 1:13893625/1‑68 (MQ=255) gTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGCGGCGCGGGGGTCAGCCCGTTAGTc > 1:1337242/1‑67 (MQ=255) gTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTcg > 1:18666794/1‑68 (MQ=255) gTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTc > 1:20681608/1‑67 (MQ=255) gTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTc > 1:11932518/1‑67 (MQ=255) tCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTcgc > 1:31734904/1‑68 (MQ=255) tCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTagc > 1:2149835/1‑68 (MQ=255) gAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAac > 1:24670581/1‑68 (MQ=255) ttCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAacgac < 1:4052615/67‑1 (MQ=255) ttCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAacga < 1:27220948/66‑1 (MQ=255) ttCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAacga < 1:15444484/66‑1 (MQ=255) tCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAAcgacga < 1:10348544/68‑1 (MQ=255) gcGCCACTTCTTGCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAAcgacga < 1:7334524/67‑1 (MQ=255) gCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAAt < 1:6941366/68‑1 (MQ=255) gCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAAt < 1:14733404/68‑1 (MQ=255) ccACTTCTTCCTGGAAGTCCCGCGCCAGAGGCGCGGGGGGCAGCCCGTTAGTCGCGGAACCACGAATg > 1:7559365/1‑68 (MQ=255) cACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGa < 1:5758200/68‑1 (MQ=255) cACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGa > 1:21055508/1‑68 (MQ=255) cACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGa < 1:16135900/68‑1 (MQ=255) cACTTCTGCCTGGAAGTCCCGCGCCGGCGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGa < 1:7477458/68‑1 (MQ=255) ttcttcCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGatat < 1:17645481/68‑1 (MQ=255) ttcttcCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGatat < 1:28357071/68‑1 (MQ=255) tcttcCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATc < 1:22754387/68‑1 (MQ=255) tcttcCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATc < 1:8571932/68‑1 (MQ=255) tcttcCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATc < 1:2438851/68‑1 (MQ=255) tcttcCTGGAAGTCCCGCGCCGGAGGCGCGGGGGTCAGACCGTTATTCGCGGAACGCCGAATGata > 1:18892002/1‑66 (MQ=255) cttcCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCt < 1:4267520/68‑1 (MQ=255) ccTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTga < 1:16386436/68‑1 (MQ=255) ggAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGAt > 1:29069486/1‑68 (MQ=255) ggAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGAt > 1:12620971/1‑68 (MQ=255) gAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGAtt > 1:14492324/1‑68 (MQ=255) aGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTg < 1:14633708/67‑1 (MQ=255) aGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGa < 1:14140750/68‑1 (MQ=255) aGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGa < 1:6345725/68‑1 (MQ=255) ccGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAg < 1:13182166/68‑1 (MQ=255) ccGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAg < 1:25085126/68‑1 (MQ=255) cgcgCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGc < 1:12763154/68‑1 (MQ=255) cgcgCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGc < 1:11078347/68‑1 (MQ=255) cgcgCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGc < 1:3735730/68‑1 (MQ=255) cgcgCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGc < 1:18946298/68‑1 (MQ=255) cgcgCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGc < 1:31484635/68‑1 (MQ=255) cgcgCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGc < 1:21339633/68‑1 (MQ=255) cgcgCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGc < 1:30232256/68‑1 (MQ=255) cgcgCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGc < 1:22602004/68‑1 (MQ=255) cgcgCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGc < 1:22976037/68‑1 (MQ=255) cgcgCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGc < 1:23920216/68‑1 (MQ=255) cgcgCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGc < 1:28581286/68‑1 (MQ=255) gcgcCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGcc < 1:27952788/68‑1 (MQ=255) cTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCata < 1:13634417/67‑1 (MQ=255) tGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATg < 1:27554448/68‑1 (MQ=255) | CTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATG > CP009273/4075990‑4076122 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |