| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 4,264,257 | A→G | 11.4% | S69G (AGC→GGC) ‡ | ssb → | single‑stranded DNA‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 4,264,257 | 0 | A | G | 11.4% | 1894.5 / 168.6 | 612 | S69G (AGC→GGC) ‡ | ssb | single‑stranded DNA‑binding protein |
| Reads supporting (aligned to +/- strand): ref base A (204/338); new base G (23/47); total (227/385) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-01 | |||||||||||
Only 100 of 655 total aligned reads displayed.
CGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCG > CP009273/4264193‑4264323 | cGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGgcgagc < 1:5322742/67‑1 (MQ=255) gagaTGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGgcgagcga < 1:28136513/68‑1 (MQ=255) tGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAata < 1:10321942/67‑1 (MQ=255) tGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAata > 1:4917973/1‑67 (MQ=255) aGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGc < 1:4498300/68‑1 (MQ=255) aGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGc < 1:30882640/68‑1 (MQ=255) aGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGc < 1:2978611/68‑1 (MQ=255) aGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGc < 1:28023666/68‑1 (MQ=255) aGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGc < 1:21459004/68‑1 (MQ=255) aGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGc < 1:18811762/68‑1 (MQ=255) aGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGc < 1:14027951/68‑1 (MQ=255) aGAACAGACTGAATGCCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGc < 1:10829238/68‑1 (MQ=255) gagcagacagaATGGCATCGCGTGGTGCTGTTCGGCAAGCTCGCGGAAGTGGCAGGTGAATGTCTGc > 1:9804242/4‑67 (MQ=255) gagcagacagaATGGCATCGCGTGGTGCTGTTCGGCAAGCTCGCGGAAGTGGCAGGTGAATGTCTGc < 1:8405143/64‑1 (MQ=255) gagcagacagaATGGCATCGCGTGGTGCTGTTCGGCAAGCTCGCGGAAGTGGCAGGTGAATGTCTGc < 1:15731443/64‑1 (MQ=255) gAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCg > 1:6331382/1‑68 (MQ=255) agcagacagaATGGCATCGCGTGGTGCTGTTCGGCAAGCTCGCGGAAGTGGCAGGTGAATGTCTGCgc > 1:29442169/3‑67 (MQ=255) aaCAGACTGAATGGCCCCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGt > 1:15468293/1‑68 (MQ=255) aaCAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGt > 1:21817094/1‑68 (MQ=255) aaCAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGt > 1:5115449/1‑68 (MQ=255) gcagacagaATGGCATCGCGTGGTGCTGTTCGGCAAGCTCGCGGAAGTGGCAGGTGAATGTCTGCgca > 1:21535806/2‑66 (MQ=255) gcagacagaATGGCATCGCGTGGTGCTGTTCGGCAAGCTCGCGGAAGTGGCAGGTGAATGTCTGCgca > 1:8795905/2‑66 (MQ=255) aCAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTa > 1:2859353/1‑68 (MQ=255) cAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTaa > 1:24968965/1‑68 (MQ=255) aGACAGAATGGCATCGCGTGGTGCTGTTCGGCAAGCTCGCGGAAGTGGCAGGTGAATGTCTGCGCaag > 1:20117946/1‑67 (MQ=255) aCTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAgg > 1:17278315/1‑68 (MQ=255) aCTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAgg > 1:12109162/1‑68 (MQ=255) aCTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAgg > 1:19441091/1‑68 (MQ=255) cagAATGGCATCGCGTGGTGCTGTTCGGCAAGCTCGCGGAAGTGGCAGGTGAATGTCTGCGCaag < 1:22168340/63‑2 (MQ=255) cTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGt < 1:2755849/68‑1 (MQ=255) cTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGt < 1:13117476/68‑1 (MQ=255) cTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGt > 1:30297757/1‑68 (MQ=255) cTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGt < 1:8625059/68‑1 (MQ=255) tGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCa > 1:30223684/1‑68 (MQ=255) tGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCa < 1:12572386/68‑1 (MQ=255) tGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCa > 1:31467162/1‑68 (MQ=255) ggCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAg > 1:545609/1‑68 (MQ=255) gCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTATCAgg > 1:14221438/1‑68 (MQ=255) cACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAgg < 1:28353853/67‑1 (MQ=255) cACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAgg < 1:6814192/67‑1 (MQ=255) cACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAgg < 1:29228529/67‑1 (MQ=255) cACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAgg < 1:25275858/67‑1 (MQ=255) cACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAgg < 1:16753144/67‑1 (MQ=255) cACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAgg < 1:8227062/67‑1 (MQ=255) cACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAgg < 1:7892882/67‑1 (MQ=255) cACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAgg < 1:20661061/67‑1 (MQ=255) aCCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGtt > 1:12261922/1‑68 (MQ=255) aCCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGtt > 1:17050625/1‑68 (MQ=255) aCCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGtt > 1:23765523/1‑68 (MQ=255) aCCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGtt > 1:17989455/1‑68 (MQ=255) aCCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGtt > 1:22797061/1‑68 (MQ=255) aCCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGtt > 1:364087/1‑68 (MQ=255) aCCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGtt > 1:928316/1‑68 (MQ=255) aCCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGtt > 1:14449315/1‑68 (MQ=255) aCCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGtt > 1:9478890/1‑68 (MQ=255) aCCGCGTTGTGATGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGtt > 1:4172347/1‑68 (MQ=255) tcgcgTGGTGCTGTTCGGCAAGCTCGCGGAAGTGGCAGGTGAATGTCTGCGCAAGGGCGCGCAGGtct < 1:29954662/67‑3 (MQ=255) gcgTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTtat < 1:7674510/68‑1 (MQ=255) gcgTGGTGCTGTTCGGCAAGCTCGCGGAAGTGGCAGGTGAATGTCTGCGCAAGGGCGCGCAGGTCtac < 1:25792120/68‑2 (MQ=255) gcgTGGTGCTGTTCGGCAAGCTCGCGGAAGTGGCAGGTGAATGTCTGCGCAAGGGCGCGCAGGTCtac < 1:2107051/68‑2 (MQ=255) ggtgCTGTTCGGCAAGCTCGCGGAAGTGGCAGGTGAATGTCTGCGCAAGGGCGCGCAGGTCTACATc < 1:23030066/66‑1 (MQ=255) gCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAg > 1:22489614/1‑68 (MQ=255) tGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAgg < 1:26133107/67‑1 (MQ=255) tGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAgg < 1:14593325/67‑1 (MQ=255) tCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCa < 1:31024424/67‑1 (MQ=255) cGGCAAGCTCGCGGAAGTGGCAGGTGAATGTCTGCGCAAGGGCGCGCAGGTCTACATCGAAGGTCAGc < 1:5814165/68‑1 (MQ=255) cGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGc < 1:21369915/68‑1 (MQ=255) cGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGc < 1:13492340/68‑1 (MQ=255) ggCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCt < 1:32013124/68‑1 (MQ=255) ggCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCt < 1:2738448/68‑1 (MQ=255) ggCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCt < 1:26587505/68‑1 (MQ=255) aaaCTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCg > 1:28842477/1‑68 (MQ=255) aaaCTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCg > 1:10054196/1‑68 (MQ=255) aaaCTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCg > 1:1050890/1‑68 (MQ=255) aaaCTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCg > 1:7208726/1‑68 (MQ=255) aaaCTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCg > 1:11083130/1‑68 (MQ=255) aaaCTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCg > 1:12829715/1‑68 (MQ=255) tGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTAcc < 1:3950514/68‑1 (MQ=255) tGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTAcc < 1:15049621/68‑1 (MQ=255) tGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTAcc < 1:18415846/68‑1 (MQ=255) tGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTAcc < 1:18588170/68‑1 (MQ=255) tGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTAcc < 1:26795314/68‑1 (MQ=255) tGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTAcc < 1:19116661/68‑1 (MQ=255) tGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTAcc < 1:6552900/68‑1 (MQ=255) tGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTAcc < 1:2337004/68‑1 (MQ=255) tGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTAcc < 1:23522643/68‑1 (MQ=255) tGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTAcc < 1:24140275/68‑1 (MQ=255) tGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTAcc < 1:24544880/68‑1 (MQ=255) tGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGGTCTCAGGTTTATATCGAAGGTCAGCTGCGTAcc < 1:6053273/68‑1 (MQ=255) ggCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTAccc < 1:19644848/68‑1 (MQ=255) ggCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTAccc < 1:31756607/68‑1 (MQ=255) ggAAGTGGCAGGTGAATGTCTGCGCAAGGGCGCGCAGGTCTACATCGAAGGTCAGCTTCGCACCCGTa < 1:26426689/67‑1 (MQ=255) aGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTa > 1:15313079/1‑68 (MQ=255) gAAGTGGCAGGTGAATGTCTGCGCAAGGGCGCGCAGGTCTACATCGAAGGTCAGCTTCGCACCCGTa > 1:16228793/1‑67 (MQ=255) aGTGGCGAGCGTATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTa < 1:16426784/65‑1 (MQ=255) aGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTa < 1:7444501/65‑1 (MQ=255) aGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTa < 1:11503975/65‑1 (MQ=255) aGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTa < 1:30630983/65‑1 (MQ=255) ggCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATgg < 1:11854594/67‑1 (MQ=255) gagcgaATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGAcc < 1:17573618/67‑1 (MQ=255) | CGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCG > CP009273/4264193‑4264323 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |