BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	692,040	G→A	7.3%	noncoding (33/75 nt)	glnV ←	tRNA‑Gln

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	692,040	0	G	A	7.3%	1299.8 / 48.2	456	noncoding (33/75 nt)	glnV	tRNA‑Gln
Reads supporting (aligned to +/- strand): ref base G (259/150); new base A (6/26); total (270/186)
Fisher's exact test for biased strand distribution p-value = 9.24e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 466 total aligned reads displayed.

TACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGA  >  CP009273/691973‑692106

tACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAg                                                                    >  1:22541063/1‑68 (MQ=255)

tACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAg                                                                    >  1:15047026/1‑68 (MQ=255)

tACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAg                                                                    >  1:15919173/1‑68 (MQ=255)

   aaGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGaa                                                                  >  1:16393003/1‑67 (MQ=255)

     ggTAAGCGTCTTGCATAAATTGGCTGGGGTACGAGGATTCGAACCACGGAATGCCGGAATCAAAATcc                                                               >  1:5721277/1‑68 (MQ=18)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                               >  1:20053862/1‑68 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                               >  1:8106189/1‑68 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                               >  1:5871991/1‑68 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                               >  1:3755016/1‑68 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                               >  1:3468865/1‑68 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCACAATcc                                                               >  1:24973911/1‑68 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGCAATCAGAATcc                                                               >  1:4374978/1‑68 (MQ=255)

       tAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCgg                                                             >  1:2327246/1‑68 (MQ=255)

        aaGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGt                                                            <  1:25736194/68‑1 (MQ=255)

        aaGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGt                                                            <  1:12517579/68‑1 (MQ=14)

        aaGCGGCTTTATTAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGt                                                            <  1:2953541/68‑1 (MQ=14)

           gagcttttttaatgTGGCTGGGGTACGAGGATTCGAACCTGGGAATGCCGGTATCAAAAACCGGt                                                            <  1:17672232‑M2/51‑1 (MQ=255)

           gagcttttttaatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGc                                                          <  1:2873515‑M2/53‑1 (MQ=255)

           gagcttttttaatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGc                                                          <  1:5411198‑M2/53‑1 (MQ=255)

           gagcttttttaatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGc                                                          <  1:26820389‑M2/53‑1 (MQ=255)

           gagcttttttaatgTGGCTGGGGTACGAGGATTCGAACCACGGAATGCCGGTATCAAAAACCGGTGcc                                                         <  1:10235025‑M2/54‑1 (MQ=255)

           ccgcttttttaattTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGcc                                                         <  1:19769470‑M2/54‑1 (MQ=255)

           cGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTg                                                           >  1:15671186/1‑66 (MQ=255)

           cGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTg                                                           >  1:12821067/1‑66 (MQ=255)

           cGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTg                                                           >  1:1112218/1‑66 (MQ=255)

           cGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTg                                                           >  1:10827051/1‑66 (MQ=255)

           cGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTg                                                           >  1:20905599/1‑66 (MQ=255)

            ggcTTGATTAATTTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGcc                                                         <  1:31965845/65‑1 (MQ=11)

            gTCTTGAATAACTTGGCTGGGGTACGAGGATTCGAACCTGGGAATGCCGGTATCAAAAACCGGTGc                                                          <  1:25172247/66‑1 (MQ=14)

            gTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCt                                                        >  1:14205348/1‑68 (MQ=255)

            gTCTTGAATAAATTGGCTGGGGTAAGAGGATTCGAACCTCGGAATGCCGGTATCaaaaa                                                                 >  1:21713802/1‑58 (MQ=18)

             tCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCtt                                                       >  1:2599929/1‑68 (MQ=255)

             tCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCtt                                                       >  1:14587871/1‑68 (MQ=255)

                tttttaatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGTATCAAAAACCGGTGCCTTAcc                                                    <  1:19180422‑M2/59‑1 (MQ=255)

                tttttaatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTAcc                                                    <  1:31238474‑M2/59‑1 (MQ=255)

                tttttaatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTAcc                                                    <  1:24328662‑M2/59‑1 (MQ=255)

                tttttaatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTAcc                                                    <  1:16816852‑M2/59‑1 (MQ=255)

                ttaaTAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTAcc                                                    <  1:10156623/66‑1 (MQ=255)

                 ttttaatgTGGCTGGGGTACGTGGATTCGAACCTCGGAATGCCGGTATCAAAAACCGGTGc                                                          <  1:13060211‑M2/53‑1 (MQ=255)

                 gAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTAcc                                                    >  1:23674838/1‑67 (MQ=255)

                  tttaatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGc                                                  <  1:4070915‑M2/61‑1 (MQ=255)

                   ttgatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCt                                                 <  1:17374331‑M2/62‑1 (MQ=255)

                   ttaattTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCt                                                 <  1:28925582‑M2/62‑1 (MQ=255)

                   ttaatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCgcc                                                 <  1:6214987‑M2/62‑2 (MQ=255)

                   ttaatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCt                                                 >  1:8001349‑M2/7‑68 (MQ=255)

                   ttaatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCt                                                 >  1:27223339‑M2/7‑68 (MQ=255)

                   ttaatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCt                                                 <  1:6850304‑M2/62‑1 (MQ=255)

                   ttaatgTGGCTGGGGTAAGAGGATTCGAACCTCGGAATGCAGGAATCAGAATAAGGTGCATTACCGCt                                                 >  1:22151039‑M2/7‑68 (MQ=255)

                   aTAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCt                                                 >  1:31583100/1‑68 (MQ=40)

                    taatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCtt                                                >  1:1365617‑M2/6‑68 (MQ=255)

                      atgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTgg                                              <  1:25547885‑M2/65‑1 (MQ=255)

                      atgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTgg                                              <  1:18734974‑M2/65‑1 (MQ=255)

                      atgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCCGTGCCTTACCGCTTgg                                              <  1:16021277‑M2/65‑1 (MQ=255)

                       tgTGGCTGGGGTACGTGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                             >  1:843956‑M2/3‑68 (MQ=255)

                       tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGc                                             <  1:17245716‑M2/66‑1 (MQ=255)

                       tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCatgtc                                             >  1:9084865‑M2/3‑66 (MQ=255)

                       tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                             <  1:22444776‑M2/66‑1 (MQ=255)

                       tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                             >  1:7392690‑M2/3‑68 (MQ=255)

                       tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                             >  1:19382776‑M2/3‑68 (MQ=255)

                       tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                             >  1:30012335‑M2/3‑68 (MQ=255)

                       tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                             >  1:12116521‑M2/3‑68 (MQ=255)

                       tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                             >  1:22711209‑M2/3‑68 (MQ=255)

                       tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                             >  1:4775069‑M2/3‑68 (MQ=255)

                       tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                             >  1:27900217‑M2/3‑68 (MQ=255)

                       tgTGGCTGGGGTACGAGGATTCGAACCACGGAATGCCGGAATCACAAACCGGTGCCTTACCGCTTGGc                                             <  1:21538601‑M2/66‑1 (MQ=255)

                       agTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                             <  1:14078194‑M2/66‑1 (MQ=255)

                       aTTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGc                                             <  1:23074141/68‑1 (MQ=11)

                       aTTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                             >  1:1531929/1‑68 (MQ=33)

                        ttGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCg                                            >  1:114380/1‑68 (MQ=31)

                        ttGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCg                                            <  1:10548031/68‑1 (MQ=31)

                        ttGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCg                                            >  1:1235650/1‑68 (MQ=31)

                        ttGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCg                                            >  1:30587427/1‑68 (MQ=31)

                        ttGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCg                                            >  1:823900/1‑68 (MQ=31)

                        ttGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCg                                            >  1:13513897/1‑68 (MQ=31)

                                   aCGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCcaaca                                 >  1:15473119/1‑68 (MQ=31)

                                      aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCcaacatcg                              >  1:13859851/1‑65 (MQ=14)

                                      aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCcaacaggt                              >  1:1176110/1‑65 (MQ=14)

                                      aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAt                              >  1:26474755/1‑68 (MQ=37)

                                      aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAt                              >  1:29302597/1‑68 (MQ=37)

                                      aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAt                              >  1:28308002/1‑68 (MQ=37)

                                      aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAt                              >  1:27713598/1‑68 (MQ=37)

                                      aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGAGTGGCGATTCCCCAACAAAt                              >  1:30796076/1‑68 (MQ=11)

                                      aGGATTCGAACCTCGGAATCCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACaaaa                              >  1:1793687/1‑67 (MQ=255)

                                        gATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTg                            >  1:27548092/1‑68 (MQ=40)

                                         aTTCGAACCTCGGACTGCCGGAATCCGAATCCGGTGCCCTACCGCTTGGCGATACCCCAACAAATTgg                           <  1:21858029/68‑1 (MQ=14)

                                          ttCGAACCTCGGAATGCCGGTATAAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGt                          <  1:8760012/68‑1 (MQ=255)

                                          ttCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTgg                           <  1:971596/67‑1 (MQ=255)

                                          ttCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTgg                           <  1:3341993/67‑1 (MQ=255)

                                          ttCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTgg                           <  1:2078761/67‑1 (MQ=255)

                                          ttCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGt                          <  1:30928609/68‑1 (MQ=255)

                                                      aaTGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGc               <  1:24530255/67‑1 (MQ=255)

                                                        tGCCGGAATCAGAATCCGGGGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGa            <  1:30178111/68‑1 (MQ=255)

                                                         gCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGaa           <  1:26762259/68‑1 (MQ=255)

                                                         gCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGa            >  1:10705674/1‑67 (MQ=255)

                                                           cGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACa         <  1:7317033/68‑1 (MQ=255)

                                                            ggAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACat        <  1:6418315/68‑1 (MQ=255)

                                                            ggAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACat        <  1:21059638/68‑1 (MQ=255)

                                                              aaTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACata       <  1:3177452/67‑1 (MQ=255)

                                                              aaTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACata       <  1:20432080/67‑1 (MQ=255)

                                                               aTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACatat      <  1:27045362/67‑1 (MQ=255)

TACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGA  >  CP009273/691973‑692106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`