| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 4,264,274 | A→G | 7.3% | K74K (AAA→AAG) | ssb → | single‑stranded DNA‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 4,264,274 | 0 | A | G | 7.3% | 2042.2 / 95.9 | 598 | K74K (AAA→AAG) | ssb | single‑stranded DNA‑binding protein |
| Reads supporting (aligned to +/- strand): ref base A (189/364); new base G (12/32); total (201/397) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.09e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01 | |||||||||||
Only 100 of 696 total aligned reads displayed.
AGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATC > CP009273/4264207‑4264341 | cTGAATGGCACCGGGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGC‑GATAATCTGCGTAAAgga > 1:12788758/1‑67 (MQ=255) cTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGt < 1:26189163/68‑1 (MQ=255) cTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGt > 1:295107/1‑68 (MQ=255) gAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTtc < 1:31305864/68‑1 (MQ=255) aTGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTtctc > 1:26659666/1‑68 (MQ=255) tGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCa > 1:30223684/1‑68 (MQ=255) tGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCa > 1:13174818/1‑68 (MQ=255) tGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCa > 1:30726652/1‑68 (MQ=255) cACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAgg < 1:21018917/67‑1 (MQ=255) cACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAgg < 1:20065133/67‑1 (MQ=255) cACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAgg < 1:28353853/67‑1 (MQ=255) cACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAgg < 1:16753144/67‑1 (MQ=255) cACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAgg < 1:30367413/67‑1 (MQ=255) cACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAgg < 1:3795009/67‑1 (MQ=255) cACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAgg < 1:11963393/67‑1 (MQ=255) atcgcgTGGTGCTGTTCGGCAAGCTCGCGGAAGTGGCAGGT‑GAATGTCTGCGCAAGGGCGCGCAGGt < 1:24788885/65‑1 (MQ=255) atcgcgTGGTGCTGTTCGGCAAGCTCGCGGAAGTGGCAGGT‑GAATGTCTGCGCAAGGGCCCGCAGGtc > 1:14266986/3‑67 (MQ=255) aCCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGtt > 1:22766905/1‑68 (MQ=255) aCCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGtt > 1:23765523/1‑68 (MQ=255) aCCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGtt > 1:28932829/1‑68 (MQ=255) aCCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGtt > 1:10687452/1‑68 (MQ=255) aCCGCGTTGTGCTGTTCGGCAAACAGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGtt > 1:6395839/1‑68 (MQ=255) tcgcgTGGTGCTGTTCGGCAAGCTCGCGGAAGTGGCAGGT‑GAATGTCTGCGCAAGGGCGCGCAGGtct < 1:29954662/67‑3 (MQ=255) gttgtgttgtTCGGCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTtata > 1:594416/1‑67 (MQ=255) gCTGTTCGGCAAGCTCGCGGAAGTGGC‑AGCTGAATGTCTGCGCAAGGGCGCGCAGGTCTACATCGCAg > 1:27432937/1‑68 (MQ=255) gCTGTTCGGCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAg > 1:14089703/1‑68 (MQ=255) gCTGTTCGGCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAg > 1:2418963/1‑68 (MQ=255) gCTGTTCGGCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAg > 1:25611183/1‑68 (MQ=255) gCTGTTCGGCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAg > 1:12210184/1‑68 (MQ=255) gCTGTTCGGCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAg > 1:15412552/1‑68 (MQ=255) gTTCGGCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTc > 1:11150156/1‑68 (MQ=255) tCGGCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCa > 1:22905496/1‑67 (MQ=255) tCGGCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCa > 1:9417694/1‑67 (MQ=255) cGGCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGc < 1:10405971/68‑1 (MQ=255) gggaaaCAGGCAGAAGTGGCGAGC‑TAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCt < 1:14965199/65‑1 (MQ=255) ggCAAGCTCGCGGAAGTGGCAGGT‑GAATGTCTGCGCAAGGGCGCGCAGGTCTACATCGAAGGTCAGCt < 1:16348491/68‑1 (MQ=255) ggCAAGCTCGCGGAAGTGGCAGGT‑GAATGTCTGCGCAAGGGCGCGCAGGTCTACATCGAAGGTCAGCt < 1:8245500/68‑1 (MQ=255) ggCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCt < 1:26498781/68‑1 (MQ=255) ggCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCt < 1:5168427/68‑1 (MQ=255) ggCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCt < 1:5361969/68‑1 (MQ=255) ggCAAACTGGCAGAAGGGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCt < 1:16985285/68‑1 (MQ=255) aaaCTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCg > 1:10054196/1‑68 (MQ=255) aaaCTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCg > 1:15684436/1‑68 (MQ=255) aaCTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGt < 1:7009450/68‑1 (MQ=255) aaCTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGt < 1:7228983/68‑1 (MQ=255) ttgCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTAcc < 1:11713835/66‑1 (MQ=255) tGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTAcc < 1:26922702/68‑1 (MQ=255) tGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTAcc < 1:17696113/68‑1 (MQ=255) tGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTAcc < 1:8725175/68‑1 (MQ=255) tGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTAcc < 1:7947521/68‑1 (MQ=255) tGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTAcc < 1:25937652/68‑1 (MQ=255) tGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTAcc < 1:17414590/68‑1 (MQ=255) tGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTAcc < 1:1853785/68‑1 (MQ=255) tGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTAcc < 1:32006993/68‑1 (MQ=255) tGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTAcc < 1:6191884/68‑1 (MQ=255) tGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTAcc < 1:4474829/68‑1 (MQ=255) tGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTAcc < 1:665200/68‑1 (MQ=255) ggCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTAccc < 1:31756607/68‑1 (MQ=255) aaGTGGCAGGT‑GAATGTCTGCGCAAGGGCGCGCAGGTCTACATCGAAGGTCAGCTTCGCACCCGTa > 1:21396678/1‑66 (MQ=255) aGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTa < 1:21255799/65‑1 (MQ=255) aGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTa < 1:31012438/65‑1 (MQ=255) aGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTa < 1:14583098/65‑1 (MQ=255) aGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTa < 1:3980759/65‑1 (MQ=255) aGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTa < 1:19643859/65‑1 (MQ=255) aGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTa < 1:27926032/65‑1 (MQ=255) aGTGGCAGGT‑GAATGTCTGCGCAAGGGCGCGCAGGTCTACATCGAAGGTCAGCTTCGCACCCGTagct < 1:28551459/68‑4 (MQ=255) aGTGGCAGGG‑GAATGTCTGCGCAAGGGCGCGCAGGTCTACATCGAAGGTCAGCTTCGCACCCGTagc < 1:10915552/67‑3 (MQ=255) gc‑gaATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGa < 1:905116/67‑1 (MQ=255) gc‑gaATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGa < 1:23383485/67‑1 (MQ=255) atatCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCaa < 1:9702192/67‑1 (MQ=255) tCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCg > 1:22296348/1‑68 (MQ=255) tCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCg > 1:22500898/1‑68 (MQ=255) tCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCg > 1:15984853/1‑68 (MQ=255) cTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCgg < 1:18255106/68‑1 (MQ=255) cTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCgg < 1:20554688/68‑1 (MQ=255) cTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCgg < 1:28099698/68‑1 (MQ=255) cTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCgg < 1:3370379/68‑1 (MQ=255) cTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCgg < 1:7587506/68‑1 (MQ=255) cTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCgg < 1:19049570/68‑1 (MQ=255) cTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCgg < 1:29365701/68‑1 (MQ=255) cTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCgg < 1:5644897/68‑1 (MQ=255) cTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCgg < 1:19363302/68‑1 (MQ=255) tGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGt < 1:25238082/68‑1 (MQ=255) gTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAg < 1:8459697/68‑1 (MQ=255) aaaGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGa < 1:26040498/68‑1 (MQ=255) aaaGGTTCTCAGGTTTATATCGAAGGGCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGa < 1:1712782/68‑1 (MQ=255) aaaGGTTCTCAGGTCTATATCGAAGATCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGa < 1:23207003/68‑1 (MQ=255) aGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATc < 1:18016880/68‑1 (MQ=255) aGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATc < 1:23531503/68‑1 (MQ=255) aGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATc < 1:4951467/68‑1 (MQ=255) aGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATc < 1:4215599/68‑1 (MQ=255) aGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATc < 1:18739100/68‑1 (MQ=255) aGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATc < 1:7771163/68‑1 (MQ=255) aGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATc < 1:21892536/68‑1 (MQ=255) aGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATc < 1:2158027/68‑1 (MQ=255) aGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATc < 1:13684920/68‑1 (MQ=255) aGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATc < 1:21481643/68‑1 (MQ=255) aGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATc < 1:24470838/68‑1 (MQ=255) aGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATc < 1:25030552/68‑1 (MQ=255) aGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATc < 1:15284657/68‑1 (MQ=255) | AGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATC > CP009273/4264207‑4264341 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |