| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 4,264,278 | T→G | 6.7% | S76A (TCT→GCT) ‡ | ssb → | single‑stranded DNA‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 4,264,278 | 0 | T | G | 6.7% | 2231.7 / 111.0 | 671 | S76A (TCT→GCT) ‡ | ssb | single‑stranded DNA‑binding protein |
| Reads supporting (aligned to +/- strand): ref base T (203/422); new base G (10/35); total (214/457) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.85e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.14e-01 | |||||||||||
Only 100 of 714 total aligned reads displayed.
GAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATCGCT > CP009273/4264212‑4264344 | aTGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTtctc > 1:11591911/1‑68 (MQ=255) tGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCa < 1:12572386/68‑1 (MQ=255) gCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAgg > 1:20072621/1‑68 (MQ=255) gCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAgg > 1:29495552/1‑68 (MQ=255) cACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAgg < 1:24652625/67‑1 (MQ=255) aCCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGtt > 1:10687452/1‑68 (MQ=255) aCCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGtt > 1:28285190/1‑68 (MQ=255) aCCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGtt > 1:3147708/1‑68 (MQ=255) aCCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGtt > 1:1214220/1‑68 (MQ=255) aCCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGtt > 1:14449315/1‑68 (MQ=255) aCCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGtt > 1:23223951/1‑68 (MQ=255) aCCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGtt > 1:5692669/1‑68 (MQ=255) tcgcgTGGTGCTGTTCGGCAAGCTCGCGGAAGTGGCAGGT‑GAATGTCTGCGCAAGGGCGCGCAGGtct < 1:29954662/67‑3 (MQ=255) ccGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGttt < 1:844027/68‑1 (MQ=255) gcgTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTtat < 1:23437762/68‑1 (MQ=255) gcgTGGTGCTGTTCGGCAAGCTCGCGGAAGTGGCAGGT‑GAATGTCTGCGCAAGGGCGCGCAGGTCtac < 1:8700415/68‑2 (MQ=255) gttgtgttgtTCGGCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTtata > 1:594416/1‑67 (MQ=255) gTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTtata > 1:21552291/1‑67 (MQ=255) gTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTtata > 1:2147523/1‑67 (MQ=255) tgtgCTGTTCGGCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATc < 1:29344925/67‑1 (MQ=255) gCTGTTCGGCAAGCTCGCGGAAGTGGC‑AGCTGAATGTCTGCGCAAGGGCGCGCAGGTCTACATCGCAg > 1:27432937/1‑68 (MQ=255) gCTGTTCGGCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAg > 1:22489614/1‑68 (MQ=255) gCTGTTCGGCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAg > 1:17212995/1‑68 (MQ=255) cTGTTCGGCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAgg > 1:22130736/1‑68 (MQ=255) tGTTCGGCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAgg < 1:26133107/67‑1 (MQ=255) tGTTCGGCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGt < 1:18863352/68‑1 (MQ=255) tGTTCGGCAAACTGACAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAgg > 1:4244066/1‑67 (MQ=255) tCGGCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCa > 1:6568347/1‑67 (MQ=255) tCGGCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCa < 1:31024424/67‑1 (MQ=255) tCGGCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCa > 1:25984386/1‑67 (MQ=255) tCGGCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCa > 1:9417694/1‑67 (MQ=255) cGGCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGc < 1:15304597/68‑1 (MQ=255) cGGCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGc < 1:13492340/68‑1 (MQ=255) cGGCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGc < 1:10405971/68‑1 (MQ=255) ggCAAGCTCGCGGAAGTGGCAGGT‑GAATGTCTGCGCAAGGGCGCGCAGGTCTACATCGAAGGTCAGCt < 1:8245500/68‑1 (MQ=255) ggCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCt < 1:11954449/68‑1 (MQ=255) aaaCTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCg > 1:10054196/1‑68 (MQ=255) aaaCTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCg > 1:28842477/1‑68 (MQ=255) aaaCTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCg > 1:4042809/1‑68 (MQ=255) aaCTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGt < 1:7228983/68‑1 (MQ=255) aaCTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGt < 1:1807712/68‑1 (MQ=255) tGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTAcc < 1:4474829/68‑1 (MQ=255) tGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTAcc < 1:26922702/68‑1 (MQ=255) tGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTAcc < 1:28093146/68‑1 (MQ=255) tGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTAcc < 1:15758032/68‑1 (MQ=255) tGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTAcc < 1:5190392/68‑1 (MQ=255) tGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTAcc < 1:1397613/68‑1 (MQ=255) tGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTAcc < 1:17474983/68‑1 (MQ=255) tGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTAcc < 1:18606576/68‑1 (MQ=255) tGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTAcc < 1:6191884/68‑1 (MQ=255) ggAAGTGGCAGGT‑GAATGTCTGCGCAAGGGCGCGCAGGTCTACATCGAAGGTCAGCTTCGCACCCGt < 1:9016853/66‑1 (MQ=255) gAAGTGGCAGGT‑GAATGTCTGCGCAAGGGCGCGCAGGTCTACATCGAAGGTCAGCTTCGCACCCGTa > 1:16228793/1‑67 (MQ=255) aGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCTTACCCGTa < 1:14204088/65‑1 (MQ=255) aGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTa < 1:26494501/65‑1 (MQ=255) aGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTa < 1:1905790/65‑1 (MQ=255) aGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTa < 1:27926032/65‑1 (MQ=255) aGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTa < 1:7444501/65‑1 (MQ=255) aGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTa < 1:30265592/65‑1 (MQ=255) aGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTa < 1:31889724/65‑1 (MQ=255) aGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTa < 1:16985651/65‑1 (MQ=255) aGTGGCAGGT‑GAATGTCTGCGCAAGGGCGCGCAGGTCTACATCGAAGGTCAGCTTCGCACCCGTagc < 1:25577310/67‑3 (MQ=255) aGTGGCAGGG‑GAATGTCTGCGCAAGGGCGCGCAGGTCTACATCGAAGGTCAGCTTCGCACCCGTagc < 1:10915552/67‑3 (MQ=255) ggCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATgg < 1:30755387/67‑1 (MQ=255) ggCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATgg > 1:24058820/1‑67 (MQ=255) gagc‑gaATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCg < 1:6398804/68‑1 (MQ=255) gagc‑gaATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCg < 1:21383568/68‑1 (MQ=255) gc‑gaATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGa > 1:14846491/1‑67 (MQ=255) atatCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCaa < 1:24967350/67‑1 (MQ=255) atatCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCaa < 1:23564286/67‑1 (MQ=255) atatCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAAt < 1:22918190/68‑1 (MQ=255) tCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCg > 1:28518400/1‑68 (MQ=255) tCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCg > 1:2188796/1‑68 (MQ=255) tCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCg > 1:18405186/1‑68 (MQ=255) cTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCgg < 1:20554688/68‑1 (MQ=255) cTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCgg < 1:5374088/68‑1 (MQ=255) cTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCgg < 1:19363302/68‑1 (MQ=255) cTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCgg < 1:16363798/68‑1 (MQ=255) cTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCgg < 1:258503/68‑1 (MQ=255) cTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCgg < 1:30440269/68‑1 (MQ=255) tGCATAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCgg > 1:17832411/1‑67 (MQ=255) aaaGGTTCTGAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGa < 1:19649203/68‑1 (MQ=255) aaaGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGa < 1:324144/68‑1 (MQ=255) aaaGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGa < 1:16799993/68‑1 (MQ=255) aGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATc < 1:7771163/68‑1 (MQ=255) aGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATc < 1:796625/68‑1 (MQ=255) aGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATc < 1:15471145/68‑1 (MQ=255) aGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATc < 1:21144321/68‑1 (MQ=255) aGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATc < 1:5051988/68‑1 (MQ=255) aGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATc < 1:22720738/68‑1 (MQ=255) aGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATc < 1:3802263/68‑1 (MQ=255) aGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATc < 1:3448847/68‑1 (MQ=255) aGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATc < 1:24390160/68‑1 (MQ=255) aGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATc < 1:25163583/68‑1 (MQ=255) aGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATc < 1:26654893/68‑1 (MQ=255) ggTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATc > 1:20766026/1‑67 (MQ=255) ggTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATc > 1:6843001/1‑67 (MQ=255) ggTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATc > 1:4812544/1‑67 (MQ=255) ggTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATc > 1:13069005/1‑67 (MQ=255) tctcAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATCGCt < 1:11136602/67‑1 (MQ=255) tctcAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATCGCt < 1:9725962/67‑1 (MQ=255) | GAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGC‑GAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATCGCT > CP009273/4264212‑4264344 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |