BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	692,043	T→A	9.2%	noncoding (30/75 nt)	glnV ←	tRNA‑Gln

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	692,043	0	T	A	9.2%	1320.7 / 54.8	453	noncoding (30/75 nt)	glnV	tRNA‑Gln
Reads supporting (aligned to +/- strand): ref base T (253/155); new base A (10/35); total (263/190)
Fisher's exact test for biased strand distribution p-value = 3.36e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 462 total aligned reads displayed.

AAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACA  >  CP009273/691976‑692110

  ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGTATCAAAATcc                                                                   >  1:26554265/1‑68 (MQ=255)

  ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:17409902/1‑68 (MQ=255)

  ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:14042531/1‑68 (MQ=255)

  ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:3468865/1‑68 (MQ=255)

  ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:12778912/1‑68 (MQ=255)

  ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:1207269/1‑68 (MQ=255)

  ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:5871991/1‑68 (MQ=255)

  ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:8106189/1‑68 (MQ=255)

    tAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCgg                                                                 >  1:2327246/1‑68 (MQ=255)

    tAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCgg                                                                 >  1:28032205/1‑68 (MQ=255)

     atcgagcttttttaattTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGt                                                                <  1:22190711‑M2/51‑1 (MQ=255)

     aaGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGt                                                                <  1:12517579/68‑1 (MQ=14)

     aaGCGGCTTTATTAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGt                                                                <  1:2953541/68‑1 (MQ=14)

      aGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGt                                                                >  1:5740110/1‑67 (MQ=255)

        gagcttttttaatgTGGCTGGGGTACGAGGATTCGAACCTGGGAATGCCGGTATCAAAAACCGGt                                                                <  1:17672232‑M2/51‑1 (MQ=255)

        gagcttttttaatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGcc                                                             <  1:21721671‑M2/54‑1 (MQ=255)

        gagcttttttaatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGcc                                                             <  1:15877656‑M2/54‑1 (MQ=255)

        gagcttttttaatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGcc                                                             <  1:4909013‑M2/54‑1 (MQ=255)

        gagcttttttaatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGcc                                                             <  1:18055627‑M2/54‑1 (MQ=255)

        gagcttttttaatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGc                                                              <  1:2873515‑M2/53‑1 (MQ=255)

        gagcttttttaatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGc                                                              <  1:11390063‑M2/53‑1 (MQ=255)

        gagcttttttaatgTGGCTGGGGTACGAGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGcc                                                             <  1:3341828‑M2/54‑1 (MQ=255)

        gagcttttttaatgTGGCTGGGGTACGAGGATTCGAACCACGGAATGCCGGTATCAAAAACCGGTGcc                                                             <  1:10235025‑M2/54‑1 (MQ=255)

        ctgcttttataattTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGcc                                                             <  1:6466424‑M2/54‑1 (MQ=255)

        ccgcttttttaattTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGcc                                                             <  1:19769470‑M2/54‑1 (MQ=255)

        cGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTg                                                               >  1:20784367/1‑66 (MQ=255)

        cGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTg                                                               >  1:22636670/1‑66 (MQ=255)

        cGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTg                                                               >  1:30574775/1‑66 (MQ=255)

        cGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTg                                                               >  1:10827051/1‑66 (MQ=255)

        cGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTg                                                               >  1:14200753/1‑66 (MQ=255)

        cGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAAACCGGTg                                                               >  1:2195304/1‑66 (MQ=255)

         ggcTTGATTAATTTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGcc                                                             <  1:31965845/65‑1 (MQ=11)

         gTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTGGGAATGCCGGTATCAAAAACCGGTGcc                                                             <  1:16366051/67‑1 (MQ=17)

         gTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTGGGAATGCCGGTATCAAAAACCGGTGcc                                                             <  1:25039432/67‑1 (MQ=17)

          tCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCtt                                                           >  1:14587871/1‑68 (MQ=255)

          tCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCtt                                                           >  1:27058707/1‑68 (MQ=255)

          gcttttttaaatTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCtt                                                           <  1:22516585‑M2/56‑1 (MQ=255)

            ttGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTg                                                               <  1:25620873/62‑1 (MQ=255)

             tttttaatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTAcc                                                        <  1:31238474‑M2/59‑1 (MQ=255)

             tttttaatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTAcc                                                        <  1:16816852‑M2/59‑1 (MQ=255)

             tttttaaagTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTAcc                                                        <  1:5412595‑M2/59‑1 (MQ=255)

             ttaaTAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTAcc                                                        <  1:10156623/66‑1 (MQ=255)

              ttttaatgTGGCTGGGGTACGTGGATTCGAACCTCGGAATGCCGGTATCAAAAACCGGTGc                                                              <  1:13060211‑M2/53‑1 (MQ=255)

               tttaatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGc                                                      <  1:4070915‑M2/61‑1 (MQ=255)

               tttaatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGc                                                      <  1:24529366‑M2/61‑1 (MQ=255)

               tttaatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGc                                                      <  1:21027802‑M2/61‑1 (MQ=255)

                ttaattTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCt                                                     <  1:28925582‑M2/62‑1 (MQ=255)

                ttaatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCgcc                                                     <  1:6214987‑M2/62‑2 (MQ=255)

                ttaatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCt                                                     >  1:8001349‑M2/7‑68 (MQ=255)

                ttaatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCt                                                     <  1:6850304‑M2/62‑1 (MQ=255)

                ttaatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCt                                                     >  1:27223339‑M2/7‑68 (MQ=255)

                aTAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCt                                                     >  1:23627769/1‑68 (MQ=40)

                aTAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCt                                                     >  1:31583100/1‑68 (MQ=40)

                aTAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGAAGGAATCAGAATCCGGTGCCTTACCGCt                                                     >  1:2683736/1‑68 (MQ=255)

                  aatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTg                                                   <  1:19953910‑M2/64‑1 (MQ=255)

                   atgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTgg                                                  <  1:18734974‑M2/65‑1 (MQ=255)

                   atgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTgg                                                  <  1:15314239‑M2/65‑1 (MQ=255)

                    tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGc                                                 <  1:17245716‑M2/66‑1 (MQ=255)

                    tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCatgtc                                                 >  1:9084865‑M2/3‑66 (MQ=255)

                    tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                                 >  1:19382776‑M2/3‑68 (MQ=255)

                    tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                                 >  1:30012335‑M2/3‑68 (MQ=255)

                    tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                                 >  1:26781585‑M2/3‑68 (MQ=255)

                    tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                                 <  1:23040970‑M2/66‑1 (MQ=255)

                    tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATAAGAATCAGGTGCCTTACCGCTTGGc                                                 >  1:27829925‑M2/3‑68 (MQ=255)

                    tgTGGCTGGGGTACGAGGATTCGAACCACGGAATGCCGGAATCACAAACCGGTGCCTTACCGCTTGGc                                                 <  1:21538601‑M2/66‑1 (MQ=255)

                    aTTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                                 >  1:7486968/1‑68 (MQ=33)

                    aTTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                                 <  1:20901362/68‑1 (MQ=33)

                     ttGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCg                                                >  1:13513897/1‑68 (MQ=31)

                     ttGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCg                                                >  1:15010018/1‑68 (MQ=31)

                     ttGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCg                                                >  1:1235650/1‑68 (MQ=31)

                     ttGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCg                                                <  1:10548031/68‑1 (MQ=31)

                     ttGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCg                                                >  1:823900/1‑68 (MQ=31)

                     ttGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCg                                                >  1:25738676/1‑68 (MQ=31)

                     gTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGTATCACAAACCGGTGCCTTACCGCTTGGCg                                                <  1:27557912‑M2/67‑1 (MQ=255)

                                aCGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCcaaca                                     >  1:15473119/1‑68 (MQ=31)

                                   aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCcatcaggg                                  >  1:3769126/1‑65 (MQ=0)

                                   aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCcaacatcg                                  >  1:13859851/1‑65 (MQ=14)

                                   aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCcaacaggt                                  >  1:1176110/1‑65 (MQ=14)

                                   aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAt                                  >  1:15985448/1‑68 (MQ=37)

                                   aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAt                                  >  1:8442158/1‑68 (MQ=37)

                                   aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAt                                  >  1:25175885/1‑68 (MQ=37)

                                   aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAt                                  >  1:24804748/1‑68 (MQ=37)

                                   aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGAGTGGCGATTCCCCAACAAAt                                  >  1:30796076/1‑68 (MQ=11)

                                       ttCGAACCTCGGAATGCCGGTATAAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGt                              <  1:8760012/68‑1 (MQ=255)

                                       ttCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTgg                               <  1:971596/67‑1 (MQ=255)

                                       ttCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGt                              <  1:4476116/68‑1 (MQ=255)

                                       ttCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGt                              <  1:11097796/68‑1 (MQ=255)

                                       ttCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGt                              <  1:30928609/68‑1 (MQ=255)

                                            aCCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGGTTTGa                         >  1:26043939/1‑68 (MQ=255)

                                             ccTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGaa                        <  1:24203437/68‑1 (MQ=255)

                                                     tGCCGGAATCAGAATCCGGGGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGa                <  1:30178111/68‑1 (MQ=255)

                                                      gCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGa                >  1:10705674/1‑67 (MQ=255)

                                                        cGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACa             <  1:7317033/68‑1 (MQ=255)

                                                         ggAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACat            <  1:29344285/68‑1 (MQ=255)

                                                         ggAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACat            <  1:1904160/68‑1 (MQ=255)

                                                           aaTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACata           <  1:3177452/67‑1 (MQ=255)

                                                           aaTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACata           <  1:20432080/67‑1 (MQ=255)

                                                            aTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACatat          <  1:13635948/67‑1 (MQ=255)

                                                                 aaTCCGGTGCCTTACCGCTTTGGGATAAACCAACAAATTGGTTGTGAATTTGCCTAGCATATTCGATa    >  1:15600080/1‑68 (MQ=25)

                                                                   tCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACa  >  1:28341258/1‑68 (MQ=255)

AAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACA  >  CP009273/691976‑692110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`