BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	692,366	A→T	5.5%	noncoding (30/75 nt)	glnU ←	tRNA‑Gln

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	692,366	0	A	T	5.5%	731.0 / 6.7	254	noncoding (30/75 nt)	glnU	tRNA‑Gln
Reads supporting (aligned to +/- strand): ref base A (136/99); new base T (2/17); total (138/116)
Fisher's exact test for biased strand distribution p-value = 5.67e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 297 total aligned reads displayed.

AACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGA  >  CP009273/692301‑692433

aaCCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAAcc                                                                   >  1:29712280/1‑68 (MQ=255)

 aCCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCg                                                                  >  1:16616744/1‑68 (MQ=255)

 aCCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCg                                                                  >  1:13225609/1‑68 (MQ=255)

 aCCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCg                                                                  >  1:26645758/1‑68 (MQ=255)

   cGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGt                                                                >  1:14223897/1‑68 (MQ=255)

      ttAGTCTAATAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGcc                                                             <  1:1700823/68‑1 (MQ=11)

      ttACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTg                                                               >  1:17123508/1‑66 (MQ=255)

         cTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCtt                                                           >  1:8009142/1‑67 (MQ=255)

         cTCGAAGAAGATGGCTGGGGTACCTGGATGCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCtt                                                           >  1:10739323/1‑67 (MQ=255)

          tCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTa                                                          >  1:21839086/1‑67 (MQ=255)

           cGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTAcc                                                        >  1:17767226/1‑68 (MQ=255)

           cGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTAcc                                                        <  1:14863945/68‑1 (MQ=255)

               gaagaTGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCtt                                                    >  1:21302924/1‑68 (MQ=35)

               gaagaTGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCtt                                                    >  1:10856476/1‑68 (MQ=35)

                aagaTGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTg                                                   >  1:88307/1‑68 (MQ=33)

                              acgtGGATTCGAACCTCGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATc                                      <  1:10103532/64‑1 (MQ=11)

                              aCCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATca                                     >  1:17963569/1‑67 (MQ=18)

                              aCCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATc                                      <  1:16329506/67‑1 (MQ=33)

                                gtGGATTCGAACCTGGGAATGCCGGTATCATAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGt                                   <  1:20144511/67‑1 (MQ=14)

                                gtGGATTCGAACCTGGGAATGCCGGTATCACAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGt                                   <  1:29991568/67‑1 (MQ=14)

                                gtGGATTCGAACCTGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGt                                   <  1:24882638/67‑1 (MQ=255)

                                gaggATTCGAACCTCGGAATGCCGGTATCATAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGt                                   <  1:6027588/66‑1 (MQ=11)

                                cTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAttca                                    >  1:19101537/1‑64 (MQ=14)

                                cTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGt                                   <  1:21191399/68‑1 (MQ=39)

                                cTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGt                                   <  1:13992920/68‑1 (MQ=39)

                                cTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGt                                   <  1:5187718/68‑1 (MQ=39)

                                cTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGt                                   <  1:21533839/68‑1 (MQ=39)

                                cTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGt                                   >  1:11688754/1‑68 (MQ=39)

                                 tGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATGCgg                                   >  1:1936291/1‑66 (MQ=9)

                                 tGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGt                                   >  1:11409448/1‑67 (MQ=39)

                                 tGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGt                                   >  1:22050209/1‑67 (MQ=39)

                                 tGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGt                                   >  1:12306956/1‑67 (MQ=39)

                                 tGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGt                                   >  1:4469295/1‑67 (MQ=39)

                                  ggATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCGGTAc                                 >  1:22830077/1‑68 (MQ=255)

                                  ggATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTAc                                 >  1:16222828/1‑68 (MQ=255)

                                   gATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACa                                >  1:31775909/1‑68 (MQ=255)

                                   gATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACa                                >  1:4217996/1‑68 (MQ=255)

                                     ttCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAAc                              >  1:9870265/1‑68 (MQ=255)

                                      tCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAAc                              >  1:27389749/1‑67 (MQ=255)

                                      tCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAAc                              >  1:15387185/1‑67 (MQ=255)

                                       cGAACCTCGGAATGCCGGAATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGc                            <  1:5292169/68‑1 (MQ=14)

                                       cGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGc                            <  1:31557228/68‑1 (MQ=255)

                                         aaCCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAAGGCtt                          >  1:2946192/1‑68 (MQ=255)

                                         aaCCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCtt                          >  1:27637025/1‑68 (MQ=255)

                                         aaCCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCAATACTTACAACGCtt                          >  1:8194213/1‑68 (MQ=14)

                                          aCCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCttt                         <  1:18391988/68‑1 (MQ=255)

                                           ccAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCtt                          >  1:1807033/1‑66 (MQ=255)

                                           ccAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCtt                          >  1:28122260/1‑66 (MQ=255)

                                            ctgggAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCt                       <  1:9720626/66‑1 (MQ=255)

                                            ctgggAATGCCGGAATCATAATCCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCt                       <  1:10823019/66‑1 (MQ=17)

                                            ctcggAATGCCGGTATCACAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCt                       <  1:10005062/65‑1 (MQ=255)

                                            cAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCt                       <  1:14136655/68‑1 (MQ=255)

                                            cAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCt                       <  1:8521969/68‑1 (MQ=255)

                                            cAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCt                       <  1:20414704/68‑1 (MQ=255)

                                            cAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCt                       <  1:25797216/68‑1 (MQ=255)

                                            cAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCt                       <  1:19740734/68‑1 (MQ=255)

                                              gggAATGCCGGTATCAAAATCCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTgg                     <  1:18599089/68‑1 (MQ=255)

                                               ggAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGt                    >  1:25094390/1‑68 (MQ=255)

                                               ggAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGt                    >  1:27122759/1‑68 (MQ=255)

                                               ggAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGt                    >  1:8711833/1‑68 (MQ=255)

                                               ggAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGt                    >  1:5970125/1‑68 (MQ=255)

                                               ggAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGt                    >  1:29152822/1‑68 (MQ=255)

                                               ggAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGt                    >  1:23767546/1‑68 (MQ=255)

                                                gAATGCCGGTCTCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTgg                     <  1:23072122/66‑1 (MQ=255)

                                                gAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTgg                     <  1:2105114/66‑1 (MQ=255)

                                                gAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTg                   >  1:26175882/1‑68 (MQ=255)

                                                gAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTg                   >  1:10449177/1‑68 (MQ=255)

                                                gAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTg                   >  1:31195143/1‑68 (MQ=255)

                                                gAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTg                   >  1:28358678/1‑68 (MQ=255)

                                                    gCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACACCATCCGTACAACGCTTTCTGGTGAATg               >  1:7636160/1‑68 (MQ=255)

                                                     ccGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATgg              >  1:28848386/1‑68 (MQ=255)

                                                      cGGTATCATAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATgg              <  1:23934992/67‑1 (MQ=255)

                                                      cGGTATCACAAACCGGTGCCTTACCGCTTGTCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGt             <  1:7442988/68‑1 (MQ=255)

                                                      cGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGt             >  1:28982053/1‑68 (MQ=255)

                                                      cGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGt             <  1:10361934/68‑1 (MQ=255)

                                                      cGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGt             <  1:22264856/68‑1 (MQ=255)

                                                      cGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGt             <  1:30879353/68‑1 (MQ=18)

                                                      cGGAATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGt             <  1:31104960/68‑1 (MQ=255)

                                                       ggaaTCATAATCCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTg            <  1:6653720/65‑1 (MQ=21)

                                                       ggaaTCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTg            <  1:30522645/65‑1 (MQ=255)

                                                       ggTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTg            <  1:15663182/68‑1 (MQ=255)

                                                       ggTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTg            <  1:24520715/68‑1 (MQ=255)

                                                       ggTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTg            <  1:32094537/68‑1 (MQ=255)

                                                           tCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCggg        >  1:6228839/1‑68 (MQ=255)

                                                              aaaaCCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAgg     <  1:12548644/68‑1 (MQ=255)

                                                              aaaaCCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAgg     <  1:23500677/68‑1 (MQ=255)

                                                                 aCCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCga  >  1:1929177/1‑68 (MQ=255)

                                                                 aCCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCga  >  1:7935408/1‑68 (MQ=255)

                                                                 aCCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCga  >  1:9310964/1‑68 (MQ=255)

                                                                 aCCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCga  >  1:28620442/1‑68 (MQ=255)

                                                                 aCCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCga  >  1:7032678/1‑68 (MQ=255)

                                                                 aCCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCga  >  1:5340157/1‑68 (MQ=255)

                                                                 aCCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCga  >  1:22575256/1‑68 (MQ=255)

                                                                 aCCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCga  >  1:4559738/1‑68 (MQ=255)

                                                                 aCCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCga  >  1:13382607/1‑68 (MQ=255)

                                                                 aCCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCga  >  1:22983410/1‑68 (MQ=255)

                                                                 aCCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCga  >  1:14511344/1‑68 (MQ=255)

                                                                 aCCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCga  >  1:15224762/1‑68 (MQ=255)

                                                                 aCCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCAGGTGAATGGTGCGGGAGGCga  >  1:5623564/1‑68 (MQ=255)

                                                                 aCCGGTGCCTTACCGCTTGGCGATACACCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCga  >  1:16052483/1‑68 (MQ=255)

AACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGA  >  CP009273/692301‑692433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`