Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	CP009273	1,503,120	20 bp→AGCATTTTTACTGGCAAAAA	NA	coding (183‑202/231 nt)	yncJ ←	uncharacterized protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP009273	= 1503119	NA (NA)	4 (0.010) +AGCATTTTTACTGGCAAAAA	4/70	NT	NA	coding (203/231 nt)	yncJ	uncharacterized protein
	?	CP009273	1503140 =	NA (NA)					coding (182/231 nt)	yncJ	uncharacterized protein

GTTGTCCCGCTTCTGGTTTGCGCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  CP009273/1503096‑1503119 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TCGCGCAAACCTTCCGCCGACTCTTCCGCCACAGCACGCAATTCATCGCTGTCC  >  CP009273/1503140‑1503193                         ||||||||||||||||||||                                                       GTTGTCCCGCTTCTGGTTTGCGCGAGCATTTTTACTGGCAAAAATCGCGCAAACCTTCCGCCGACTCT                                >  1:17345707/1‑68 GTTGTCCCGCTTCTGGTTTGCGCGAGCATTTTTACTGGCAAAAATCGCGCAAACCTTCCGCCGACTCT                                <  1:20446323/68‑1      CCCGCTTCTGGTTTGCGCGAGCATTTTTACTGGCAAAAATCGCGCAAACCCTCCGCCGACTCTTCCG                            >  1:27385144/1‑67                  TTGCGCGAGCATTTTTACTGGCAAAAATCGCGCAAACCTTCCGCCGACTCTTCCGCCACAGCACGCA                <  1:16183058/67‑1                                TTACTGGCAAAAATCGCGCAAACCTTCCGCCGACTCTTCCGCCACAGAACGCAATTCATCGCTGTCC  >  1:14172011/1‑67                         ||||||||||||||||||||                                                       GTTGTCCCGCTTCTGGTTTGCGCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  CP009273/1503096‑1503119 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TCGCGCAAACCTTCCGCCGACTCTTCCGCCACAGCACGCAATTCATCGCTGTCC  >  CP009273/1503140‑1503193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.