| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 1,191,703 | A→G | 14.3% | L375L (TTA→TTG) ‡ | icd → | e14 prophage; isocitrate dehydrogenase, specific for NADP+ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 1,191,703 | 0 | A | G | 14.3% | 1251.0 / 148.0 | 405 | L375L (TTA→TTG) ‡ | icd | e14 prophage; isocitrate dehydrogenase, specific for NADP+ |
| Reads supporting (aligned to +/- strand): ref base A (176/171); new base G (17/41); total (193/212) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.74e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.75e-01 | |||||||||||
Only 100 of 408 total aligned reads displayed.
CTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGC‑TGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATG > CP009273/1191636‑1191769 | ctctATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGC‑TGACCTGATTg > 1:30403762/1‑68 (MQ=17) ctctATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGC‑TGACTTAATTg > 1:37726194/1‑68 (MQ=255) tctATTATTCTCTCCGCTTAGATGATGCTGCGCGACATGGGTTGGACCGAAGCGGC‑TGACTTAATTGt < 1:14399095/68‑1 (MQ=255) tctATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGC‑TGACCTGATTg < 1:5502603/67‑1 (MQ=17) tctATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGC‑TGACCTGATTg < 1:23005192/67‑1 (MQ=17) tctATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGC‑TGACCTGATTGt < 1:24712715/68‑1 (MQ=17) tctATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGC‑TGACTTAATTg < 1:31072420/67‑1 (MQ=255) tctATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGC‑TGACTTAATTGt < 1:30498566/68‑1 (MQ=255) tctATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGGTGGACCGAAGCGGC‑TGACTTAATTGt < 1:37990376/68‑1 (MQ=255) ctATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGC‑TGACCTGATTGtt < 1:37095369/68‑1 (MQ=17) ttattCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGC‑TGACTTAATTGTTaaa < 1:10952025/68‑1 (MQ=255) tattCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACAGAAGCGGC‑TGACTTAATTGTTAAAg > 1:2763074/1‑68 (MQ=255) attCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGC‑TGACCTGATTGTTAAAgg > 1:25975931/1‑68 (MQ=14) attCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGC‑TGACCTGATTGTTAAAgg < 1:5827515/68‑1 (MQ=14) attCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGC‑TGACCTGATTGTTAAAgg < 1:14811002/68‑1 (MQ=14) attCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGC‑TGACCTGATTGTTAAAgg < 1:13553184/68‑1 (MQ=14) attCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGC‑TGACCTGATTGTTAAAgg < 1:12187405/68‑1 (MQ=14) attCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGC‑TGACCTGATTGTTAAAgg < 1:21149081/68‑1 (MQ=14) attCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGC‑TGACTTAATTGTTAAAgg < 1:39308860/68‑1 (MQ=255) attCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGC‑TGACTTAATTGTTAAAgg < 1:4359899/68‑1 (MQ=255) attCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGC‑TGACTTAATTGTTAAAgg < 1:2133593/68‑1 (MQ=255) attCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGC‑TGACTTAATTGTTAAAgg < 1:39174069/68‑1 (MQ=255) attCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGC‑TGACTTAATTGTTAAAgg < 1:13497185/68‑1 (MQ=255) attCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGC‑TGACTTAATTGTTAAAgg < 1:11441118/68‑1 (MQ=255) attCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGC‑TGACTTAATTGTTAAAgg < 1:32340086/68‑1 (MQ=255) attCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGC‑TGACTTAATTGTTAAAgg < 1:33082999/68‑1 (MQ=255) ttCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGC‑TGACATGATTGTTAAAgg < 1:17728617/67‑1 (MQ=14) tctctcCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGC‑TGACCTGATTGTTAAAGGTa > 1:39546708/1‑68 (MQ=11) tctctcCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGC‑TGACTTAATTGTTAAAGGTa > 1:25379979/1‑68 (MQ=255) tctctcCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGC‑TGACTTAATTGTTAAAGGTa > 1:38580483/1‑68 (MQ=255) tctctcCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGC‑TGACTTAATTGTTAAAGGTa > 1:38348217/1‑68 (MQ=255) tctctcCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGC‑TGACTTAATTGTTAAAGGTa > 1:24167966/1‑68 (MQ=255) tctctcCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGC‑TGACTTAATTGTTAAAGGTa > 1:31751165/1‑68 (MQ=255) tctctcCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGC‑TGACTTAATTGTTAAAGGTa > 1:10021107/1‑68 (MQ=255) tctctcCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGC‑TGACTTAATTGTTAAAGGTa > 1:14624980/1‑68 (MQ=255) tctctcCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGC‑TGACTTAATTGTTAAAGGTa > 1:12607091/1‑68 (MQ=255) tctctcCGCTGAGATGATGCTGCGACACATGGGTTGGACCGAAGCGTC‑TGACTTAATTGTTAAAGGTa > 1:11123273/1‑68 (MQ=25) tctcCGCTGAGATGGTGCTGCGCCACATGGGTGGGACCGAAGCGGC‑TGGGTTAATTGTTAAAGGTATg > 1:28559491/1‑68 (MQ=21) tctcCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGC‑TGACTTAATTGTTAAAGGTATg > 1:5183751/1‑68 (MQ=255) tctcCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGC‑TGACTTAATTGTTAAAGGTATg > 1:19372987/1‑68 (MQ=255) tctcCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGC‑TGACTTAATTGTTAAAGGTATg > 1:9131772/1‑68 (MQ=255) tctcCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGC‑TGACTTAATTGTTAAAGGTATg > 1:15961524/1‑68 (MQ=255) tctcCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGC‑TGACTTAATTGTTAAAGGTATg > 1:6999374/1‑68 (MQ=255) tctcCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGC‑TGACTTAAGTGTTATAGGTATg > 1:15041939/1‑68 (MQ=255) gCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGC‑TGACTTAATTGTTAAAGGTATGGAAgg < 1:22835059/68‑1 (MQ=255) gCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGC‑TGACTTAATTGTTAAAGGTATGGAAgg < 1:8631323/68‑1 (MQ=255) gCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGC‑TGACTTAATTGTTAAAGGTATGGAAg > 1:1901114/1‑67 (MQ=255) gCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGC‑TGACTTAATTGTTAAAGGTATGGAAg > 1:31366794/1‑67 (MQ=255) tGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGC‑TGACTTAATTGTTAAAGGTATGGAAGgc > 1:6809873/1‑67 (MQ=255) gatgatGCTGCGCCACATGGTTTGGA‑CGAAACGGCGTGCCTTAA‑TGTTAAAGGTATTGAAGGCCCaa > 1:1631780/1‑67 (MQ=9) gatgatGCTGCGCCACATGGGTTGGACCGAAGCGGC‑TGACTTAATTGTTAAAGGTATGGAAGGCGCaa > 1:3781492/1‑68 (MQ=255) gatgatGCTGCGCCACATGGGTTGGACCGAAGCGGC‑TGACTTAATTGTTAAAGGTATGGAAGGCGCaa > 1:3355986/1‑68 (MQ=255) gatgatGCTGCGCCACATGGGTTGGACCGAAGCGGC‑TGACTTAATTGTTAAAGGTATGGAAGGCGCaa > 1:29913868/1‑68 (MQ=255) gatgatGCTGCGCCACATGGGTTGGACCGAAGCGGC‑TGACTTAATTGTTAAAGGTATGGAAGGCGCaa > 1:8226801/1‑68 (MQ=255) ctaatgCTGCGCCACATGGGTTGGACCGAAGCGGC‑TGACTTAATTGTTAAAGGTATGGAAGGCGCaa > 1:20203228/4‑67 (MQ=255) tgctgcGCCACATGGGTTGGCCCGCAGCGGC‑TGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAAc > 1:30858457/1‑68 (MQ=255) tgctgcGCCACATGGGTTGGACCGAAGCGGC‑TGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAAc > 1:17970107/1‑68 (MQ=255) gctgcGCCACATGGGTTGGACCGAAGCGGC‑TGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAAcg < 1:11873176/68‑1 (MQ=255) gctgcGCCACATGGGTTGGACCGAAGCGGC‑TGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAAc > 1:23900226/1‑67 (MQ=255) gctgcGCCACATGGGTTGGACCGAAGCGGC‑TGACTTAATTCTTAAAGGTATGGAACGCGCAATCAAc > 1:33199377/1‑67 (MQ=255) ctgcGCCACATGGGTTGGACCGAAGCGGC‑TGCCTTAATTGTTAAAGGTATGGAAGGCGCAATCAAcgc > 1:28238130/1‑68 (MQ=255) ctgcGCCACATGGGTTGGACCGAAGCGGC‑TGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAAcgc > 1:32594834/1‑68 (MQ=255) ctgcGCCACATGGGTTGGACCGAAGCGGC‑TGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAAcgc > 1:33816752/1‑68 (MQ=255) tgcGCCACATGGGTTGGACCGAAGCGGC‑TGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAAcgcg > 1:12392528/1‑68 (MQ=255) gcCACATGGGTTGGACCGAAGCGGC‑TGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGaaa > 1:14039259/1‑68 (MQ=255) gcCACATGGGTTGGACCGAAGCGGC‑TGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGaaa > 1:5372768/1‑68 (MQ=255) ccACATGGGTTGGACCGAAGCGGC‑TGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGaaa > 1:34602740/1‑67 (MQ=255) cacaTGGGTTGGACCGAAGCGGC‑TGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGaaaa < 1:10470622/67‑1 (MQ=255) cacaTGGGTTGGACCGAAGCGGC‑TGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGaaaa < 1:23464040/67‑1 (MQ=255) cacaTGGGTTGGACCGAAGCGGC‑TGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAAc < 1:10585105/68‑1 (MQ=255) cacaTGGGTTGGACCGAAGCGGC‑TGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAAc < 1:16910588/68‑1 (MQ=255) cacaTGGGTTGGACCGAAGCGGC‑TGACTTAATTGTTAAAGGGATGGAAGGCGCAATCAACGCGaaaa < 1:34755527/67‑1 (MQ=255) acaTGGGTTGGACCGAAGCGGC‑TGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAAcc < 1:29284940/68‑1 (MQ=255) gTTGGACCGAAGCGGC‑TGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAAc < 1:36739740/67‑1 (MQ=255) gTTGGACCGAAGCGGC‑TGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAAc < 1:12980751/67‑1 (MQ=255) ttGGACCGAAGCGGC‑TGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCt > 1:35758967/1‑68 (MQ=255) ttGGACCGAAGCGGC‑TGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCt > 1:7623739/1‑68 (MQ=255) tGGACCGAAGCGGC‑TGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCt < 1:19882362/67‑1 (MQ=255) tGGACCGAAGCGGC‑TGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCt < 1:14147757/67‑1 (MQ=255) tGGACCGAAGCGGC‑TGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCt < 1:22644340/67‑1 (MQ=255) tGGACCGAAGCGGC‑TGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCt < 1:27216660/67‑1 (MQ=255) gACCGAAGCGGC‑TGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATg > 1:15349689/1‑68 (MQ=255) aCCGAAGCGGC‑TGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATg > 1:38897849/1‑67 (MQ=255) aCCGAAGCGGC‑TGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATg > 1:17134628/1‑67 (MQ=255) ccGAAGCGGC‑TGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATg > 1:34036057/1‑66 (MQ=255) ccGAAGCGGC‑TGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATg > 1:7468210/1‑66 (MQ=255) aGCGGC‑TGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCg > 1:18261988/1‑68 (MQ=255) gCGGC‑TGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGa > 1:26335293/1‑68 (MQ=255) c‑TGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCg < 1:8936783/67‑1 (MQ=255) c‑TGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCg < 1:6403401/67‑1 (MQ=255) tGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGt < 1:9331259/67‑1 (MQ=255) tGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTc < 1:27910282/68‑1 (MQ=255) tGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTc < 1:30018496/68‑1 (MQ=255) tGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAAACTATGACTTCGAGCGTc < 1:2897652/68‑1 (MQ=255) gACTTAATTGTTAAAGGTATGGAAGGCGCAATCATCGCGAAAACCGTAACCTATGACTTCGAGCGTCt < 1:36177709/68‑1 (MQ=255) gACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCt < 1:16738092/68‑1 (MQ=255) gACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCt < 1:7197586/68‑1 (MQ=255) gACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCt < 1:6271227/68‑1 (MQ=255) gACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCt < 1:26929008/68‑1 (MQ=255) cTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTga < 1:22094818/68‑1 (MQ=255) | CTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGC‑TGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATG > CP009273/1191636‑1191769 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |